Summary of Study ST002502

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001616. The data can be accessed directly via it's Project DOI: 10.21228/M8TB15 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002502
Study TitleThe ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (IKIDS Cohort)
Study TypeProspective Cohort Study
Study SummaryIKIDS is a prospective birth cohort in Central Illinois. Pregnant women were recruited at two obstetrical clinics in Urbana Champaign IL from 2014-2021. Women were enrolled between 8-14 weeks of pregnancy. Eligibility criteria included 18-40 years of age, English speaking, not in a high risk pregnancy, planning to stay in the area until their child's first birth day and living within 30 minutes of the University of Illinois campus. Health, demographic and life style data and biospecimens were collected at 5 time points across pregnancy and children were followed prospectively from birth including assessments at birth, 1-5 weeks 4.5 months, 7.5 months, 2 years, 3 years, 4 years and 7.5 years of age. Physical and neurobehavioral development were assessed, with a particular focus on the impacts of prenatal chemical exposures (phthalates, phenols, PFAS) or prenatal maternal stress on child health outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. IKIDS is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
University of Illinois Urbana-Champaign
DepartmentBeckman Institute for Advanced Science and Technology
Last NameSchantz
First NameSusan
Address2325/21 Beckman Institute 405 North Mathews Avenue Urbana, IL 61801
Emailschantz@illinois.edu
Phone(217) 333-6230
Submit Date2023-03-08
Total Subjects300
Study CommentsHHEAR Project EM20-0011, ECHO Project EC0376
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Chear StudyYes
Analysis Type DetailLC-MS
Release Date2024-03-08
Release Version1
Susan Schantz Susan Schantz
https://dx.doi.org/10.21228/M8TB15
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001616
Project DOI:doi: 10.21228/M8TB15
Project Title:The ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (IKIDS Cohort)
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:This project was funded by the NIH Environmental influences on Child Health Outcomes (ECHO) Program. Untargeted metabolomics profiling data was acquired from urine samples provided by 14 ECHO cohorts.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner Lab
Last Name:Rushing
First Name:Blake
Address:Nutrition Research Institute, UNC-CH, 500 Laureate Way, Kannapolis, NC 28081
Email:blake_rushing@unc.edu
Phone:(704) 282-9838
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The IKIDS ECHO Cohort is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539

Subject:

Subject ID:SU002599
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type Batch
SA250913IKIDS_B_8Blank 1
SA250914IKIDS_B_1Blank 1
SA250915IKIDS_B_7Blank 1
SA250916IKIDS_B_6Blank 1
SA250917IKIDS_B_2Blank 1
SA250918IKIDS_B_4Blank 1
SA250919IKIDS_B_3Blank 1
SA250920IKIDS_B_5Blank 1
SA250921IKIDS_B_15Blank 2
SA250922IKIDS_B_16Blank 2
SA250923IKIDS_B_14Blank 2
SA250924IKIDS_B_10Blank 2
SA250925IKIDS_B_9Blank 2
SA250926IKIDS_B_13Blank 2
SA250927IKIDS_B_11Blank 2
SA250928IKIDS_B_12Blank 2
SA250929IKIDS_B_23Blank 3
SA250930IKIDS_B_22Blank 3
SA250931IKIDS_B_24Blank 3
SA250932IKIDS_B_18Blank 3
SA250933IKIDS_B_21Blank 3
SA250934IKIDS_B_17Blank 3
SA250935IKIDS_B_19Blank 3
SA250936IKIDS_B_20Blank 3
SA250937IKIDS_B_30Blank 4
SA250938IKIDS_B_28Blank 4
SA250939IKIDS_B_29Blank 4
SA250940IKIDS_B_27Blank 4
SA250941IKIDS_B_25Blank 4
SA250942IKIDS_B_26Blank 4
SA250943IKIDS_CHEAR_U_4CHEAR Urine Pool 1
SA250944IKIDS_CHEAR_U_2CHEAR Urine Pool 1
SA250945IKIDS_CHEAR_U_3CHEAR Urine Pool 1
SA250946IKIDS_CHEAR_U_1CHEAR Urine Pool 1
SA250947IKIDS_CHEAR_U_8CHEAR Urine Pool 2
SA250948IKIDS_CHEAR_U_5CHEAR Urine Pool 2
SA250949IKIDS_CHEAR_U_6CHEAR Urine Pool 2
SA250950IKIDS_CHEAR_U_7CHEAR Urine Pool 2
SA250951IKIDS_CHEAR_U_11CHEAR Urine Pool 3
SA250952IKIDS_CHEAR_U_12CHEAR Urine Pool 3
SA250953IKIDS_CHEAR_U_10CHEAR Urine Pool 3
SA250954IKIDS_CHEAR_U_9CHEAR Urine Pool 3
SA250955IKIDS_CHEAR_U_15CHEAR Urine Pool 4
SA250956IKIDS_CHEAR_U_14CHEAR Urine Pool 4
SA250957IKIDS_CHEAR_U_13CHEAR Urine Pool 4
SA250958IKIDS_HHEAR_U1_4HHEAR Urine Pool 1 (HHEAR_U1_20) 1
SA250959IKIDS_HHEAR_U1_1HHEAR Urine Pool 1 (HHEAR_U1_20) 1
SA250960IKIDS_HHEAR_U1_3HHEAR Urine Pool 1 (HHEAR_U1_20) 1
SA250961IKIDS_HHEAR_U1_2HHEAR Urine Pool 1 (HHEAR_U1_20) 1
SA250962IKIDS_HHEAR_U1_8HHEAR Urine Pool 1 (HHEAR_U1_20) 2
SA250963IKIDS_HHEAR_U1_7HHEAR Urine Pool 1 (HHEAR_U1_20) 2
SA250964IKIDS_HHEAR_U1_6HHEAR Urine Pool 1 (HHEAR_U1_20) 2
SA250965IKIDS_HHEAR_U1_5HHEAR Urine Pool 1 (HHEAR_U1_20) 2
SA250966IKIDS_HHEAR_U1_12HHEAR Urine Pool 1 (HHEAR_U1_20) 3
SA250967IKIDS_HHEAR_U1_9HHEAR Urine Pool 1 (HHEAR_U1_20) 3
SA250968IKIDS_HHEAR_U1_11HHEAR Urine Pool 1 (HHEAR_U1_20) 3
SA250969IKIDS_HHEAR_U1_10HHEAR Urine Pool 1 (HHEAR_U1_20) 3
SA250970IKIDS_HHEAR_U1_15HHEAR Urine Pool 1 (HHEAR_U1_20) 4
SA250971IKIDS_HHEAR_U1_14HHEAR Urine Pool 1 (HHEAR_U1_20) 4
SA250972IKIDS_HHEAR_U1_13HHEAR Urine Pool 1 (HHEAR_U1_20) 4
SA250973IKIDS_HHEAR_U2_4HHEAR Urine Pool 2 (HHEAR_U2_50) 1
SA250974IKIDS_HHEAR_U2_3HHEAR Urine Pool 2 (HHEAR_U2_50) 1
SA250975IKIDS_HHEAR_U2_2HHEAR Urine Pool 2 (HHEAR_U2_50) 1
SA250976IKIDS_HHEAR_U2_1HHEAR Urine Pool 2 (HHEAR_U2_50) 1
SA250977IKIDS_HHEAR_U2_8HHEAR Urine Pool 2 (HHEAR_U2_50) 2
SA250978IKIDS_HHEAR_U2_7HHEAR Urine Pool 2 (HHEAR_U2_50) 2
SA250979IKIDS_HHEAR_U2_6HHEAR Urine Pool 2 (HHEAR_U2_50) 2
SA250980IKIDS_HHEAR_U2_5HHEAR Urine Pool 2 (HHEAR_U2_50) 2
SA250981IKIDS_HHEAR_U2_12HHEAR Urine Pool 2 (HHEAR_U2_50) 3
SA250982IKIDS_HHEAR_U2_11HHEAR Urine Pool 2 (HHEAR_U2_50) 3
SA250983IKIDS_HHEAR_U2_10HHEAR Urine Pool 2 (HHEAR_U2_50) 3
SA250984IKIDS_HHEAR_U2_9HHEAR Urine Pool 2 (HHEAR_U2_50) 3
SA250985IKIDS_HHEAR_U2_15HHEAR Urine Pool 2 (HHEAR_U2_50) 4
SA250986IKIDS_HHEAR_U2_14HHEAR Urine Pool 2 (HHEAR_U2_50) 4
SA250987IKIDS_HHEAR_U2_13HHEAR Urine Pool 2 (HHEAR_U2_50) 4
SA250988IKIDS_NIST_3672_3NIST Urine Pool 3672 1
SA250989IKIDS_NIST_3672_2NIST Urine Pool 3672 1
SA250990IKIDS_NIST_3672_1NIST Urine Pool 3672 1
SA250991IKIDS_NIST_3672_4NIST Urine Pool 3672 2
SA250992IKIDS_NIST_3672_6NIST Urine Pool 3672 2
SA250993IKIDS_NIST_3672_5NIST Urine Pool 3672 2
SA250994IKIDS_NIST_3672_9NIST Urine Pool 3672 3
SA250995IKIDS_NIST_3672_7NIST Urine Pool 3672 3
SA250996IKIDS_NIST_3672_8NIST Urine Pool 3672 3
SA250997IKIDS_NIST_3672_11NIST Urine Pool 3672 4
SA250998IKIDS_NIST_3672_10NIST Urine Pool 3672 4
SA250999IKIDS_NIST_3672_12NIST Urine Pool 3672 4
SA251000IKIDS_SP_6Study Pool 1
SA251001IKIDS_SP_7Study Pool 1
SA251002IKIDS_SP_5Study Pool 1
SA251003IKIDS_SP_8Study Pool 1
SA251004IKIDS_SP_2Study Pool 1
SA251005IKIDS_SP_4Study Pool 1
SA251006IKIDS_SP_1Study Pool 1
SA251007IKIDS_SP_3Study Pool 1
SA251008IKIDS_SP_14Study Pool 2
SA251009IKIDS_SP_16Study Pool 2
SA251010IKIDS_SP_15Study Pool 2
SA251011IKIDS_SP_13Study Pool 2
SA251012IKIDS_SP_12Study Pool 2
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Collection:

Collection ID:CO002592
Collection Summary:IKIDS is a prospective birth cohort in Central Illinois. Pregnant women were recruited at two obstetrical clinics in Urbana Champaign IL from 2014-2021. Women were enrolled between 8-14 weeks of pregnancy. Eligibility criteria included 18-40 years of age, English speaking, not in a high risk pregnancy, planning to stay in the area until their child's first birth day and living within 30 minutes of the University of Illinois campus. Health, demographic and life style data and biospecimens were collected at 5 time points across pregnancy and children were followed prospectively from birth including assessments at birth, 1-5 weeks 4.5 months, 7.5 months, 2 years, 3 years, 4 years and 7.5 years of age. Physical and neurobehavioral development were assessed, with a particular focus on the impacts of prenatal chemical exposures (phthalates, phenols, PFAS) or prenatal maternal stress on child health outcomes.
Sample Type:Urine
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002611
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP002605
Sampleprep Summary:Study samples and study pools (50 µL aliquots) were shipped from the University of Illinois at Urbana-Champaign to the NC HHEAR Hub on dry ice. HHEAR Urine Pool 1 (50 µL each), HHEAR Urine Pool 2 (50 µL each), CHEAR reference urine (50 µL each) and NIST urine (SRM 3672) reference material (50 µL each) were provided by the NC HHEAR Hub. LC-MS grade water (50 µL) was used as blanks. All samples were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference urine, CHEAR reference urine, NIST reference urine, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:4℃
Extraction Method:Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard
Extract Storage:-80℃
Sample Resuspension:Water-Methanol (95:5, v/v)
Sample Spiking:Tryptophan-d5 stock solution at 500 ng/mL

Combined analysis:

Analysis ID AN004111
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units m/z

Chromatography:

Chromatography ID:CH003046
Chromatography Summary:Reverse phase
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Pressure:6000-10000
Column Temperature:50℃
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 mL/min
Injection Temperature:8oC
Internal Standard:Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22 min
Weak Wash Solvent Name:10% methanol/90% water; 0.1% formic acid
Strong Wash Solvent Name:75% 2-Propanol/25% Water; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS003858
Analysis ID:AN004111
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 KV
Collision Energy:20-45, ramp
Collision Gas:N2
Dry Gas Flow:55
Dry Gas Temp:400°C
Fragmentation Method:CID
Ion Spray Voltage:3.5kV
Ionization:ES+
Mass Accuracy:5 ppm
Dataformat:Profile
Desolvation Gas Flow:55
Desolvation Temperature:400 oC
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