Summary of Study ST002514

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001622. The data can be accessed directly via it's Project DOI: 10.21228/M81T5C This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002514
Study TitleThe investigation of the role of dietary inulin in NASH progression with mouse fecal metabolites
Study Typenon-targeted LC-Mass analysis
Study SummaryTo investigate the role of dietary fiber in non-alcoholic steatohepatitis (NASH) progression, male C57 mice was randomly assigned into four groups that received normal chow diet (NCD), choline deficient high fat diet (CDHFD), CDHFD + 10% inulin (CDHFD-I), CDHFD + 10% Cellulose (CDHFD-C). Additionally, some mice received inulin or cellulose was treated with 13C labelled fiber for 36 hours. Fecal metabolites were analyzed by non-targeted metabolomics.Results shown that the fecal metabolites from Inulin treatment group signficantly distinguished from that from CDHFD only group, while only negligible alterations was induced by cellulose treatment, indicating that inulin not cellulose could be well fermented by gut microbiota. The 13C tracing results shown that nineteen 13C-inulin labelled metabolites were also captured including pantothenate, phosphoethanolamine and adenosine. These metabolites are reported to play a protective role in reducing fat accumulation and ameliorating cellular oxidative stress and inflammation, indicating that the mechanism inulin suppresses NASH may through mediating modulating gut metabolites.
Institute
The Chinese University of Hong Kong
Last NameWei
First NameWenchao
AddressPrince of Wales Hospital
Email1155118538@link.cuhk.edu.hk
Phone56001913
Submit Date2023-03-17
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-05-19
Release Version1
Wenchao Wei Wenchao Wei
https://dx.doi.org/10.21228/M81T5C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001622
Project DOI:doi: 10.21228/M81T5C
Project Title:The investigation of the role of dietary inulin in Non-alcoholic Steatohepatits NASH progression
Project Type:non-targeted LC-MS analysis
Project Summary:To investigate the role of dietary fiber in non-alcoholic steatohepatitis (NASH) progression, male C57 mice was randomly assigned into four groups that received normal chow diet (NCD), choline deficient high fat diet (CDHFD), CDHFD + 10% inulin (CDHFD-I), CDHFD + 10% Cellulose (CDHFD-C). Mice stool and portal vein serum was collected. Additionally, some mice received inulin or cellulose was treated with 13C labelled fiber for 36 hours. Metabolites were analyzed by non-targeted metabolomics.
Institute:The Chinese University of Hong Kong
Department:Medicine
Last Name:Wei
First Name:Wenchao
Address:Prince of Wales Hospital
Email:1155118538@link.cuhk.edu.hk
Phone:56001913
Publications:https://www.nature.com/articles/s41564-023-01418-7

Subject:

Subject ID:SU002614
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57 BL/6
Age Or Age Range:23-24 weeks
Weight Or Weight Range:30-60g
Height Or Height Range:N/A
Gender:Male
Animal Animal Supplier:The Laboratory Animal Services Centre in the Chinese univeristy of Hong Kong
Animal Housing:The Laboratory Animal Services Centre in the Chinese univeristy of Hong Kong
Animal Light Cycle:12/12
Animal Feed:libitum
Animal Water:libitum
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Samplet_Type Treatment
SA252993F1236_posMice Stool CDHFD
SA252994F1241_posMice Stool CDHFD
SA252995F1246_posMice Stool CDHFD
SA252996F1125_negMice Stool CDHFD
SA252997F1242_posMice Stool CDHFD
SA252998F1125_posMice Stool CDHFD
SA252999F1241_negMice Stool CDHFD
SA253000F1236_negMice Stool CDHFD
SA253001F1246_negMice Stool CDHFD
SA253002F1242_negMice Stool CDHFD
SA253003F1251_negMice Stool CDHFD-13C-C
SA253004F1250_negMice Stool CDHFD-13C-C
SA253005F1249_negMice Stool CDHFD-13C-C
SA253006F1203_negMice Stool CDHFD-13C-C
SA253007F1257_negMice Stool CDHFD-13C-C
SA253008F1251_posMice Stool CDHFD-13C-C
SA253009F1259_posMice Stool CDHFD-13C-C
SA253010F1203_posMice Stool CDHFD-13C-C
SA253011F1249_posMice Stool CDHFD-13C-C
SA253012F1250_posMice Stool CDHFD-13C-C
SA253013F1259_negMice Stool CDHFD-13C-C
SA253014F1257_posMice Stool CDHFD-13C-C
SA253015F1280_posMice Stool CDHFD-13C-I
SA253016F1225_posMice Stool CDHFD-13C-I
SA253017F1225_negMice Stool CDHFD-13C-I
SA253018F1218_negMice Stool CDHFD-13C-I
SA253019F1228_negMice Stool CDHFD-13C-I
SA253020F1227_negMice Stool CDHFD-13C-I
SA253021F1218_posMice Stool CDHFD-13C-I
SA253022F1228_posMice Stool CDHFD-13C-I
SA253023F1227_posMice Stool CDHFD-13C-I
SA253024F1280_negMice Stool CDHFD-13C-I
SA253025F1254_posMice Stool CDHFD-C
SA253026F1253_posMice Stool CDHFD-C
SA253027F1247_posMice Stool CDHFD-C
SA253028F1255_negMice Stool CDHFD-C
SA253029F1255_posMice Stool CDHFD-C
SA253030F1254_negMice Stool CDHFD-C
SA253031F1253_negMice Stool CDHFD-C
SA253032F1247_negMice Stool CDHFD-C
SA253033F1222_posMice Stool CDHFD-I
SA253034F1223_posMice Stool CDHFD-I
SA253035F1230_posMice Stool CDHFD-I
SA253036F1222_negMice Stool CDHFD-I
SA253037F1217_posMice Stool CDHFD-I
SA253038F1216_posMice Stool CDHFD-I
SA253039F1230_negMice Stool CDHFD-I
SA253040F1217_negMice Stool CDHFD-I
SA253041F1216_negMice Stool CDHFD-I
SA253042F1223_negMice Stool CDHFD-I
SA253043F1264_negMice Stool NCD
SA253044F1261_posMice Stool NCD
SA253045F1263_negMice Stool NCD
SA253046F1262_negMice Stool NCD
SA253047F1267_posMice Stool NCD
SA253048F1267_negMice Stool NCD
SA253049F1261_negMice Stool NCD
SA253050F1262_posMice Stool NCD
SA253051F1263_posMice Stool NCD
SA253052F1264_posMice Stool NCD
SA253053QC04_posMice Stool QC
SA253054QC03_posMice Stool QC
SA253055QC05_posMice Stool QC
SA253056QC06_posMice Stool QC
SA253057QC02_posMice Stool QC
SA253058QC01_negMice Stool QC
SA253059QC05_negMice Stool QC
SA253060QC06_negMice Stool QC
SA253061QC04_negMice Stool QC
SA253062QC03_negMice Stool QC
SA253063QC02_negMice Stool QC
SA253064QC01_posMice Stool QC
Showing results 1 to 72 of 72

Collection:

Collection ID:CO002607
Collection Summary:Collected mouse stool was stored in liquid nitrogen immediately and stored in -80℃ for long term storage.
Collection Protocol Filename:Protocol_for_The_role_of_fiber_in_NASH_progression_stool.docx
Sample Type:Feces
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002626
Treatment Summary:Male C57 mice was randomly assigned into 4 groups that received Normal chow diet (NCD), Choline deficient high fat diet (CDHFD), CDHFD + 10% inulin (CDHFD-I), CDHFD + 10% Cellulose (CDHFD-C). Among the mice received inulin or cellulose, some of them received 13C labelled inulin or cellulose for 36 hours. Mouse stool were collected.

Sample Preparation:

Sampleprep ID:SP002620
Sampleprep Summary:25 mg of sample was weighted to an EP tube, and 500 μL extract solution (acetonitrile: methanol: water = 2: 2: 1) containing internal standard (L-2-Chlorophenylalanine, 4 μg/mL) was added. After 30 s vortex, the samples were homogenized at 35 Hz for 4 min and sonicated for 5 min in ice-water bath. The homogenization and sonication cycle was repeated for 2 times. Then the samples were incubated at -40 ℃ for 1 h and centrifuged at 12000 rpm for 15 min at 4 ℃. 400 μL of supernatant was transferred to a fresh tube and dried in a vacuum concentrator at 37 ℃. Then, the dried samples were reconstituted in 200 μL of 50% acetonitrile by sonication on ice for 10 min. The constitution was then centrifuged at 13000 rpm for 15 min at 4 ℃, and 75 μL of supernatant was transferred to a fresh glass vial for LC/MS analysis. The quality control (QC) sample was prepared by mixing an equal aliquot of the supernatants from all of the samples.
Sampleprep Protocol Filename:Protocol_for_The_role_of_fiber_in_NASH_progression_stool.docx

Combined analysis:

Analysis ID AN004140 AN004141
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity
Column Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 6600 TripleTOF ABI Sciex 6600 TripleTOF
Ion Mode POSITIVE NEGATIVE
Units Peak ion intensity Peak ion intensity

Chromatography:

Chromatography ID:CH003066
Chromatography Summary:Non-targeted metabolomic analysis was performed on a 1290 Infinity series UHPLC System (Agilent Technologies) coupled to a Triple TOF 6600 mass spectrometer (AB Sciex ) equipped with an electrospray ionization (ESI) source by Biotree company (Shanghai, China). Briefly, chromatographic separation was achieved by using a Waters BEH Amide column (2.1 * 100 mm, 1.7 µm) under 25 ℃. The mobile phase consisted of water with 25mM ammonium acetate and 25 mM ammonium hydroxide (A) and acetonitrile (B). The flow rate was 0.5 ml/min.
Methods Filename:Protocol_for_The_role_of_fiber_in_NASH_progression_stool.docx
Instrument Name:Agilent 1290 Infinity
Column Name:Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
Column Temperature:25 ℃
Flow Gradient:0~0.5 min, 95% B; 0.5~7 min, 95%~65% B; 7~8 min, 65%~40% B; 8~9 min, 40% B; 9~9.1 min, 40%~95% B; 9.1~12 min, 95% B
Flow Rate:0.5 ml/min.
Solvent A:water with 25mM ammonium acetate and 25 mM ammonium hydroxide
Solvent B:acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS003887
Analysis ID:AN004140
Instrument Name:ABI Sciex 6600 TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
MS Comments:MS analysis was operated in information-dependent basis (IDA) mode under positive. The 12 most abundant ions were selected, fragmented and sent to TOF mass analyzer to acquire the MS2 information.
Ion Mode:POSITIVE
Analysis Protocol File:Protocol_for_The_role_of_fiber_in_NASH_progression_stool.docx
  
MS ID:MS003888
Analysis ID:AN004141
Instrument Name:ABI Sciex 6600 TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
MS Comments:MS analysis was operated in information-dependent basis (IDA) mode under negative modes. The 12 most abundant ions were selected, fragmented and sent to TOF mass analyzer to acquire the MS2 information.
Ion Mode:NEGATIVE
Analysis Protocol File:Protocol_for_The_role_of_fiber_in_NASH_progression_stool.docx
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