Summary of Study ST002520

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001622. The data can be accessed directly via it's Project DOI: 10.21228/M81T5C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002520
Study TitleThe investigation of the role of dietary inulin in Non-alcoholic Steatohepatitis (NASH) progression with portal vein serum metabolites
Study Typenon-targeted LC-Mass analysis
Study SummaryTo investigate the role of dietary fiber in non-alcoholic steatohepatitis (NASH) progression, male C57 mice was randomly assigned into four groups that received normal chow diet (NCD), choline deficient high fat diet (CDHFD), CDHFD + 10% inulin (CDHFD-I), CDHFD + 10% Cellulose (CDHFD-C). Mice portal vein serum was collected.Results shown that the fecal metabolites from Inulin treatment group signficantly distinguished from that from CDHFD only group, while only negligible alterations was induced by cellulose treatment, indicating that inulin not cellulose could be well fermented by gut microbiota. By overlaping with fecal metabolites, the inulin derived pentadecaonic acid was consistently enriched in portal vein serum, which was reported as a beneficial metabolite.The results indicate that inulin suppresses NASH may through direct modulating gut metabolites.
Institute
The Chinese University of Hong Kong
Last NameWei
First NameWenchao
AddressPrince of Wales Hospital
Email1155118538@link.cuhk.edu.hk
Phone56001913
Submit Date2023-03-17
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-05-19
Release Version1
Wenchao Wei Wenchao Wei
https://dx.doi.org/10.21228/M81T5C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001622
Project DOI:doi: 10.21228/M81T5C
Project Title:The investigation of the role of dietary inulin in Non-alcoholic Steatohepatits NASH progression
Project Type:non-targeted LC-MS analysis
Project Summary:To investigate the role of dietary fiber in non-alcoholic steatohepatitis (NASH) progression, male C57 mice was randomly assigned into four groups that received normal chow diet (NCD), choline deficient high fat diet (CDHFD), CDHFD + 10% inulin (CDHFD-I), CDHFD + 10% Cellulose (CDHFD-C). Mice stool and portal vein serum was collected. Additionally, some mice received inulin or cellulose was treated with 13C labelled fiber for 36 hours. Metabolites were analyzed by non-targeted metabolomics.
Institute:The Chinese University of Hong Kong
Department:Medicine
Last Name:Wei
First Name:Wenchao
Address:Prince of Wales Hospital
Email:1155118538@link.cuhk.edu.hk
Phone:56001913
Publications:https://www.nature.com/articles/s41564-023-01418-7

Subject:

Subject ID:SU002620
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57 BL/6
Age Or Age Range:23-24 weeks
Weight Or Weight Range:30-60g
Height Or Height Range:N/A
Gender:Male
Animal Animal Supplier:The Laboratory Animal Services Centre in the Chinese univeristy of Hong Kong
Animal Housing:The Laboratory Animal Services Centre in the Chinese univeristy of Hong Kong
Animal Light Cycle:12/12
Animal Feed:libitum
Animal Water:libitum

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA253988X3773posCDHFD
SA253989X3772posCDHFD
SA253990X3774posCDHFD
SA253991X3777posCDHFD
SA253992X3780posCDHFD
SA253993X3771negCDHFD
SA253994X3775posCDHFD
SA253995X3771posCDHFD
SA253996X3777negCDHFD
SA253997X3774negCDHFD
SA253998X3773negCDHFD
SA253999X3772negCDHFD
SA254000X3780negCDHFD
SA254001X3775negCDHFD
SA254002X3792posCDHFD-C
SA254003X3794posCDHFD-C
SA254004X3791posCDHFD-C
SA254005X3791negCDHFD-C
SA254006X3792negCDHFD-C
SA254007X3796posCDHFD-C
SA254008X3793posCDHFD-C
SA254009X3794negCDHFD-C
SA254010X3800posCDHFD-C
SA254011X3796negCDHFD-C
SA254012X3800negCDHFD-C
SA254013X3793negCDHFD-C
SA254014X3785posCDHFD-I
SA254015X3784posCDHFD-I
SA254016X3786posCDHFD-I
SA254017X3788posCDHFD-I
SA254018X3786negCDHFD-I
SA254019X3787negCDHFD-I
SA254020X3790posCDHFD-I
SA254021X3787posCDHFD-I
SA254022X3781posCDHFD-I
SA254023X3788negCDHFD-I
SA254024X3784negCDHFD-I
SA254025X3781negCDHFD-I
SA254026X3790negCDHFD-I
SA254027X3785negCDHFD-I
SA254028X3717posNCD
SA254029X3718negNCD
SA254030X3718posNCD
SA254031X3717negNCD
SA254032X3729posNCD
SA254033X3727posNCD
SA254034X3725posNCD
SA254035X3725negNCD
SA254036X3727negNCD
SA254037X3729negNCD
SA254038QC04posQC
SA254039QC03posQC
SA254040QC05posQC
SA254041QC05negQC
SA254042QC02posQC
SA254043QC04negQC
SA254044QC01negQC
SA254045QC02negQC
SA254046QC03negQC
SA254047QC01posQC
Showing results 1 to 60 of 60

Collection:

Collection ID:CO002613
Collection Summary:Mice portal vein serum were collected under anaesthesia. Serum was processed by centrifugation with 3000 rpm for 15 min after coagulation. Processed serum was stored in liquid nitrogen immediately and stored in -80°C fridge.
Collection Protocol Filename:Protocol_of_the_role_of_dietary_inulin_in_NASH_progression_portal_vein_serum.docx
Sample Type:Blood (serum)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002632
Treatment Summary:Male C57 mice was randomly assigned into 4 groups that received Normal chow diet (NCD), Choline deficient high fat diet (CDHFD), CDHFD + 10% inulin (CDHFD-I), CDHFD + 10% Cellulose (CDHFD-C). Mice portal vein serum were collected.

Sample Preparation:

Sampleprep ID:SP002626
Sampleprep Summary:50 μL of sample was transferred to an EP tube, and 200 μL extract solution (acetonitrile: methanol = 1: 1) containing internal standard (L-2-Chlorophenylalanine, 2 μg/mL) was added. After 1 min vortex, the samples were sonicated for 10 min in ice-water bath. Then the samples were incubated at -40 ℃ for 1 h and centrifuged at 12000 rpm for 15 min at 4 ℃. 200 μL of supernatant was transferred to a fresh tube and dried in a vacuum concentrator at 37 ℃. Then, the dried samples were reconstituted in 200 μL of 50% acetonitrile by sonication on ice for 10 min. The constitution was then centrifuged at 13000 rpm for 15 min at 4 ℃, and 75 μL of supernatant was transferred to a fresh glass vial for LC/MS analysis. The quality control (QC) sample was prepared by mixing an equal aliquot of the supernatants from all of the samples.
Sampleprep Protocol Filename:Protocol_of_the_role_of_dietary_inulin_in_NASH_progression_portal_vein_serum.docx

Combined analysis:

Analysis ID AN004151 AN004152
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity
Column Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 6600 TripleTOF ABI Sciex 6600 TripleTOF
Ion Mode POSITIVE NEGATIVE
Units Peak ion intensity Peak ion intensity

Chromatography:

Chromatography ID:CH003072
Chromatography Summary:Non-targeted metabolomic analysis was performed on a 1290 Infinity series UHPLC System (Agilent Technologies) coupled to a Triple TOF 6600 mass spectrometer (AB Sciex ) equipped with an electrospray ionization (ESI) source by Biotree company (Shanghai, China). Briefly, chromatographic separation was achieved by using a Waters BEH Amide column (2.1 * 100 mm, 1.7 µm) under 25 ℃. The mobile phase consisted of water with 25mM ammonium acetate and 25 mM ammonium hydroxide (A) and acetonitrile (B). The flow rate was 0.5 ml/min.
Methods Filename:Protocol_of_the_role_of_dietary_inulin_in_NASH_progression_portal_vein_serum.docx
Instrument Name:Agilent 1290 Infinity
Column Name:Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
Column Temperature:25 ℃
Flow Gradient:0~0.5 min, 95% B; 0.5~7 min, 95%~65% B; 7~8 min, 65%~40% B; 8~9 min, 40% B; 9~9.1 min, 40%~95% B; 9.1~12 min, 95% B
Flow Rate:0.5 ml/min.
Solvent A:100% water; 25mM ammonium acetate; 25 mM ammonium hydroxide
Solvent B:100% acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS003898
Analysis ID:AN004151
Instrument Name:ABI Sciex 6600 TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
MS Comments:MS analysis was operated in information-dependent basis (IDA) mode under positive modes. The 12 most abundant ions were selected, fragmented and sent to TOF mass analyzer to acquire the MS2 information.
Ion Mode:POSITIVE
Analysis Protocol File:Protocol_of_the_role_of_dietary_inulin_in_NASH_progression_portal_vein_serum.docx
  
MS ID:MS003899
Analysis ID:AN004152
Instrument Name:ABI Sciex 6600 TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
MS Comments:MS analysis was operated in information-dependent basis (IDA) mode under negative modes. The 12 most abundant ions were selected, fragmented and sent to TOF mass analyzer to acquire the MS2 information.
Ion Mode:NEGATIVE
Analysis Protocol File:Protocol_of_the_role_of_dietary_inulin_in_NASH_progression_portal_vein_serum.docx
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