Summary of Study ST002583

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002583
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Plasma - Targeted Tricarboxylic Acid Cycle
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Mayo Clinic
DepartmentInternal Medicine
LaboratoryMayo Clinic Metabolomics Resource Core (MCMRC)
Last NameNair
First NameK. Sreekumaran
Address200 1st St SW, Rochester, MN 55902
Emailnair@mayo.edu
Phone(507) 284-2511
Submit Date2023-04-25
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailGC-MS
Release Date2023-10-06
Release Version1
K. Sreekumaran Nair K. Sreekumaran Nair
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002685
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA25911590245013110Control 8 weeks of training or control time Female
SA25911690248013110Control 8 weeks of training or control time Female
SA25911790265013110Control 8 weeks of training or control time Female
SA25911890266013110Control 8 weeks of training or control time Female
SA25911990252013110Control 8 weeks of training or control time Female
SA25912090232013110Control 8 weeks of training or control time Male
SA25912190237013110Control 8 weeks of training or control time Male
SA25912290239013110Control 8 weeks of training or control time Male
SA25912390229013110Control 8 weeks of training or control time Male
SA25912490217013110Control 8 weeks of training or control time Male
SA25912590564013110Training 1 week of training or control time Female
SA25912690567013110Training 1 week of training or control time Female
SA25912790571013110Training 1 week of training or control time Female
SA25912890559013110Training 1 week of training or control time Female
SA25912990560013110Training 1 week of training or control time Female
SA25913090426013110Training 1 week of training or control time Male
SA25913190421013110Training 1 week of training or control time Male
SA25913290430013110Training 1 week of training or control time Male
SA25913390422013110Training 1 week of training or control time Male
SA25913490423013110Training 1 week of training or control time Male
SA25913590587013110Training 2 weeks of training Female
SA25913690585013110Training 2 weeks of training Female
SA25913790578013110Training 2 weeks of training Female
SA25913890581013110Training 2 weeks of training Female
SA25913990576013110Training 2 weeks of training Female
SA25914090439013110Training 2 weeks of training Male
SA25914190444013110Training 2 weeks of training Male
SA25914290441013110Training 2 weeks of training Male
SA25914390450013110Training 2 weeks of training Male
SA25914490449013110Training 2 weeks of training Male
SA25914590416013110Training 4 weeks of training Female
SA25914690420013110Training 4 weeks of training Female
SA25914790412013110Training 4 weeks of training Female
SA25914890410013110Training 4 weeks of training Female
SA25914990406013110Training 4 weeks of training Female
SA25915090292013110Training 4 weeks of training Male
SA25915190280013110Training 4 weeks of training Male
SA25915290281013110Training 4 weeks of training Male
SA25915390289013110Training 4 weeks of training Male
SA25915490283013110Training 4 weeks of training Male
SA25915590267013110Training 8 weeks of training or control time Female
SA25915690259013110Training 8 weeks of training or control time Female
SA25915790258013110Training 8 weeks of training or control time Female
SA25915890251013110Training 8 weeks of training or control time Female
SA25915990254013110Training 8 weeks of training or control time Female
SA25916090227013110Training 8 weeks of training or control time Male
SA25916190218013110Training 8 weeks of training or control time Male
SA25916290222013110Training 8 weeks of training or control time Male
SA25916390223013110Training 8 weeks of training or control time Male
SA25916490225013110Training 8 weeks of training or control time Male
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Collection:

Collection ID:CO002678
Collection Summary:-
Sample Type:Plasma

Treatment:

Treatment ID:TR002697
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002691
Sampleprep Summary:homogenized in PBS, isotope IS added, deproteinized, 2 steps derivatization with ethoxamine, MTBSTFA
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004254
Analysis type MS
Chromatography type GC
Chromatography system Agilent Intuvo 9000 GC
Column Agilent HP-5MS (30m x 0.250mm, 0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5977B HES MSD
Ion Mode POSITIVE
Units micromolar

Chromatography:

Chromatography ID:CH003160
Chromatography Summary:Analytes were chromatographically separated with 17 minutes GC gradient
Methods Filename:pass1b_tca_methods.pdf
Instrument Name:Agilent Intuvo 9000 GC
Column Name:Agilent HP-5MS (30m x 0.250mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS004001
Analysis ID:AN004254
Instrument Name:Agilent 5977B HES MSD
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:SIM; peak integration, concentrations calculated
Ion Mode:POSITIVE
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