Summary of Study ST002597

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002597
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Gastrocnemius - Targeted Tricarboxylic Acid Cycle
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Mayo Clinic
DepartmentInternal Medicine
LaboratoryMayo Clinic Metabolomics Resource Core (MCMRC)
Last NameNair
First NameK. Sreekumaran
Address200 1st St SW, Rochester, MN 55902
Emailnair@mayo.edu
Phone(507) 284-2511
Submit Date2023-04-25
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailGC-MS
Release Date2023-10-06
Release Version1
K. Sreekumaran Nair K. Sreekumaran Nair
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002699
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA25982590266015516Control 8 weeks of training or control time Female
SA25982690245015516Control 8 weeks of training or control time Female
SA25982790248015516Control 8 weeks of training or control time Female
SA25982890265015516Control 8 weeks of training or control time Female
SA25982990252015516Control 8 weeks of training or control time Female
SA25983090239015516Control 8 weeks of training or control time Male
SA25983190237015516Control 8 weeks of training or control time Male
SA25983290229015516Control 8 weeks of training or control time Male
SA25983390232015516Control 8 weeks of training or control time Male
SA25983490217015516Control 8 weeks of training or control time Male
SA25983590559015516Training 1 week of training or control time Female
SA25983690560015516Training 1 week of training or control time Female
SA25983790564015516Training 1 week of training or control time Female
SA25983890567015516Training 1 week of training or control time Female
SA25983990571015516Training 1 week of training or control time Female
SA25984090430015516Training 1 week of training or control time Male
SA25984190421015516Training 1 week of training or control time Male
SA25984290423015516Training 1 week of training or control time Male
SA25984390426015516Training 1 week of training or control time Male
SA25984490422015516Training 1 week of training or control time Male
SA25984590587015516Training 2 weeks of training Female
SA25984690585015516Training 2 weeks of training Female
SA25984790576015516Training 2 weeks of training Female
SA25984890578015516Training 2 weeks of training Female
SA25984990581015516Training 2 weeks of training Female
SA25985090449015516Training 2 weeks of training Male
SA25985190450015516Training 2 weeks of training Male
SA25985290444015516Training 2 weeks of training Male
SA25985390441015516Training 2 weeks of training Male
SA25985490439015516Training 2 weeks of training Male
SA25985590406015516Training 4 weeks of training Female
SA25985690420015516Training 4 weeks of training Female
SA25985790410015516Training 4 weeks of training Female
SA25985890412015516Training 4 weeks of training Female
SA25985990416015516Training 4 weeks of training Female
SA25986090280015516Training 4 weeks of training Male
SA25986190281015516Training 4 weeks of training Male
SA25986290289015516Training 4 weeks of training Male
SA25986390292015516Training 4 weeks of training Male
SA25986490283015516Training 4 weeks of training Male
SA25986590251015516Training 8 weeks of training or control time Female
SA25986690254015516Training 8 weeks of training or control time Female
SA25986790267015516Training 8 weeks of training or control time Female
SA25986890258015516Training 8 weeks of training or control time Female
SA25986990259015516Training 8 weeks of training or control time Female
SA25987090222015516Training 8 weeks of training or control time Male
SA25987190218015516Training 8 weeks of training or control time Male
SA25987290225015516Training 8 weeks of training or control time Male
SA25987390227015516Training 8 weeks of training or control time Male
SA25987490223015516Training 8 weeks of training or control time Male
Showing results 1 to 50 of 50

Collection:

Collection ID:CO002692
Collection Summary:-
Sample Type:Gastrocnemius

Treatment:

Treatment ID:TR002711
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002705
Sampleprep Summary:homogenized in PBS, isotope IS added, deproteinized, 2 steps derivatization with ethoxamine, MTBSTFA
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004269
Analysis type MS
Chromatography type GC
Chromatography system Agilent Intuvo 9000 GC
Column Agilent DB5-MS (30m x 0.25mm, 0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5977B HES MSD
Ion Mode POSITIVE
Units nano moles per vial

Chromatography:

Chromatography ID:CH003175
Chromatography Summary:Analytes were chromatographically separated with 17 minutes GC gradient
Methods Filename:pass1b_tca_methods.pdf
Instrument Name:Agilent Intuvo 9000 GC
Column Name:Agilent DB5-MS (30m x 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS004016
Analysis ID:AN004269
Instrument Name:Agilent 5977B HES MSD
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:SIM; peak integration, concentrations calculated
Ion Mode:POSITIVE
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