Summary of Study ST002607

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002607
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Kidney - Targeted Tricarboxylic Acid Cycle
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Mayo Clinic
DepartmentInternal Medicine
LaboratoryMayo Clinic Metabolomics Resource Core (MCMRC)
Last NameNair
First NameK. Sreekumaran
Address200 1st St SW, Rochester, MN 55902
Emailnair@mayo.edu
Phone(507) 284-2511
Submit Date2023-04-28
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailGC-MS
Release Date2023-10-06
Release Version1
K. Sreekumaran Nair K. Sreekumaran Nair
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002709
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26047590266015911Control 8 weeks of training or control time Female
SA26047690245015911Control 8 weeks of training or control time Female
SA26047790248015911Control 8 weeks of training or control time Female
SA26047890265015911Control 8 weeks of training or control time Female
SA26047990252015911Control 8 weeks of training or control time Female
SA26048090239015911Control 8 weeks of training or control time Male
SA26048190237015911Control 8 weeks of training or control time Male
SA26048290229015911Control 8 weeks of training or control time Male
SA26048390232015911Control 8 weeks of training or control time Male
SA26048490217015911Control 8 weeks of training or control time Male
SA26048590559015911Training 1 week of training or control time Female
SA26048690560015911Training 1 week of training or control time Female
SA26048790564015911Training 1 week of training or control time Female
SA26048890567015911Training 1 week of training or control time Female
SA26048990571015911Training 1 week of training or control time Female
SA26049090430015911Training 1 week of training or control time Male
SA26049190421015911Training 1 week of training or control time Male
SA26049290423015911Training 1 week of training or control time Male
SA26049390426015911Training 1 week of training or control time Male
SA26049490422015911Training 1 week of training or control time Male
SA26049590587015911Training 2 weeks of training Female
SA26049690585015911Training 2 weeks of training Female
SA26049790576015911Training 2 weeks of training Female
SA26049890578015911Training 2 weeks of training Female
SA26049990581015911Training 2 weeks of training Female
SA26050090449015911Training 2 weeks of training Male
SA26050190450015911Training 2 weeks of training Male
SA26050290444015911Training 2 weeks of training Male
SA26050390441015911Training 2 weeks of training Male
SA26050490439015911Training 2 weeks of training Male
SA26050590406015911Training 4 weeks of training Female
SA26050690420015911Training 4 weeks of training Female
SA26050790410015911Training 4 weeks of training Female
SA26050890412015911Training 4 weeks of training Female
SA26050990416015911Training 4 weeks of training Female
SA26051090280015911Training 4 weeks of training Male
SA26051190281015911Training 4 weeks of training Male
SA26051290289015911Training 4 weeks of training Male
SA26051390292015911Training 4 weeks of training Male
SA26051490283015911Training 4 weeks of training Male
SA26051590251015911Training 8 weeks of training or control time Female
SA26051690254015911Training 8 weeks of training or control time Female
SA26051790267015911Training 8 weeks of training or control time Female
SA26051890258015911Training 8 weeks of training or control time Female
SA26051990259015911Training 8 weeks of training or control time Female
SA26052090222015911Training 8 weeks of training or control time Male
SA26052190218015911Training 8 weeks of training or control time Male
SA26052290225015911Training 8 weeks of training or control time Male
SA26052390227015911Training 8 weeks of training or control time Male
SA26052490223015911Training 8 weeks of training or control time Male
Showing results 1 to 50 of 50

Collection:

Collection ID:CO002702
Collection Summary:-
Sample Type:Kidney

Treatment:

Treatment ID:TR002719
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002715
Sampleprep Summary:homogenized in PBS, isotope IS added, deproteinized, 2 steps derivatization with ethoxamine, MTBSTFA
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004279
Analysis type MS
Chromatography type GC
Chromatography system Agilent Intuvo 9000 GC
Column HP-5MS, 30 x 0.250 x 250um
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5977B HES MSD
Ion Mode POSITIVE
Units nano moles per vial

Chromatography:

Chromatography ID:CH003185
Chromatography Summary:Analytes were chromatographically separated with 17 minutes GC gradient
Methods Filename:pass1b_tca_methods.pdf
Instrument Name:Agilent Intuvo 9000 GC
Column Name:HP-5MS, 30 x 0.250 x 250um
Chromatography Type:GC

MS:

MS ID:MS004026
Analysis ID:AN004279
Instrument Name:Agilent 5977B HES MSD
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:SIM; peak integration, concentrations calculated
Ion Mode:POSITIVE
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