Summary of Study ST002610

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002610
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Lung - Targeted Oxylipins
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Emory University
DepartmentBiochemistry
LaboratoryIntegrated Lipidomics and Metabolomics Core
Last NameOrtlund
First NameEric
Address1510 Clifton Rd. NE, Room G235, Atlanta, GA 30322
Emaileortlun@emory.edu
Phone(404) 727-5014
Submit Date2023-04-28
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Eric Ortlund Eric Ortlund
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002712
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26065390248016608Control 8 weeks of training or control time Female
SA26065490252016608Control 8 weeks of training or control time Female
SA26065590265016608Control 8 weeks of training or control time Female
SA26065690266016608Control 8 weeks of training or control time Female
SA26065790245016608Control 8 weeks of training or control time Female
SA26065890237016608Control 8 weeks of training or control time Male
SA26065990239016608Control 8 weeks of training or control time Male
SA26066090217016608Control 8 weeks of training or control time Male
SA26066190232016608Control 8 weeks of training or control time Male
SA26066290229016608Control 8 weeks of training or control time Male
SA260663QC_blank_69QC-Blank 0 hour -
SA260664QC_blank_68QC-Blank 0 hour -
SA260665QC_blank_79QC-Blank 0 hour -
SA260666QC_blank_78QC-Blank 0 hour -
SA260667QC_blank_65QC-Blank 0 hour -
SA260668QC_blank_4QC-Blank 0 hour -
SA260669QC_Standard_70QC-ExternalStandard 0 hour -
SA260670QC_Standard_75QC-ExternalStandard 0 hour -
SA260671QC_Standard_76QC-ExternalStandard 0 hour -
SA260672QC_Standard_77QC-ExternalStandard 0 hour -
SA260673QC_Standard_74QC-ExternalStandard 0 hour -
SA260674QC_Standard_73QC-ExternalStandard 0 hour -
SA260675QC_Standard_71QC-ExternalStandard 0 hour -
SA260676QC_Standard_72QC-ExternalStandard 0 hour -
SA260677QC_62QC-Pooled 0 hour -
SA260678QC_67QC-Pooled 0 hour -
SA260679QC_51QC-Pooled 0 hour -
SA260680QC_40QC-Pooled 0 hour -
SA260681QC_7QC-Pooled 0 hour -
SA260682QC_18QC-Pooled 0 hour -
SA260683QC_29QC-Pooled 0 hour -
SA260684QC_prerun_3QC-PreRun 0 hour -
SA260685QC_prerun_2QC-PreRun 0 hour -
SA260686QC_prerun_1QC-PreRun 0 hour -
SA260687G_QC_2_6QC-Reference 0 hour -
SA260688G_QC_2_64QC-Reference 0 hour -
SA260689QC_SRMQC-Reference 0 hour -
SA260690G_QC_1_63QC-Reference 0 hour -
SA260691G_QC_1_5QC-Reference 0 hour -
SA26069290571016608Training 1 week of training or control time Female
SA26069390559016608Training 1 week of training or control time Female
SA26069490567016608Training 1 week of training or control time Female
SA26069590560016608Training 1 week of training or control time Female
SA26069690564016608Training 1 week of training or control time Female
SA26069790426016608Training 1 week of training or control time Male
SA26069890423016608Training 1 week of training or control time Male
SA26069990421016608Training 1 week of training or control time Male
SA26070090430016608Training 1 week of training or control time Male
SA26070190422016608Training 1 week of training or control time Male
SA26070290585016608Training 2 weeks of training Female
SA26070390587016608Training 2 weeks of training Female
SA26070490581016608Training 2 weeks of training Female
SA26070590576016608Training 2 weeks of training Female
SA26070690578016608Training 2 weeks of training Female
SA26070790450016608Training 2 weeks of training Male
SA26070890439016608Training 2 weeks of training Male
SA26070990449016608Training 2 weeks of training Male
SA26071090441016608Training 2 weeks of training Male
SA26071190444016608Training 2 weeks of training Male
SA26071290412016608Training 4 weeks of training Female
SA26071390410016608Training 4 weeks of training Female
SA26071490406016608Training 4 weeks of training Female
SA26071590420016608Training 4 weeks of training Female
SA26071690416016608Training 4 weeks of training Female
SA26071790280016608Training 4 weeks of training Male
SA26071890283016608Training 4 weeks of training Male
SA26071990281016608Training 4 weeks of training Male
SA26072090292016608Training 4 weeks of training Male
SA26072190289016608Training 4 weeks of training Male
SA26072290267016608Training 8 weeks of training or control time Female
SA26072390259016608Training 8 weeks of training or control time Female
SA26072490251016608Training 8 weeks of training or control time Female
SA26072590258016608Training 8 weeks of training or control time Female
SA26072690254016608Training 8 weeks of training or control time Female
SA26072790227016608Training 8 weeks of training or control time Male
SA26072890218016608Training 8 weeks of training or control time Male
SA26072990223016608Training 8 weeks of training or control time Male
SA26073090225016608Training 8 weeks of training or control time Male
SA26073190222016608Training 8 weeks of training or control time Male
Showing results 1 to 79 of 79

Collection:

Collection ID:CO002705
Collection Summary:-
Sample Type:Lung

Treatment:

Treatment ID:TR002722
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002718
Sampleprep Summary:purification of oxylipids with SPE column
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004282
Analysis type MS
Chromatography type Reversed phase
Chromatography system ExionLC AC
Column ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap
Ion Mode NEGATIVE
Units ng/uL

Chromatography:

Chromatography ID:CH003188
Chromatography Summary:Lipids were separated by liquid chromatography with 20 minutes gradient
Methods Filename:pass1b_oxylipneg_etamidpos_methods.pdf
Instrument Name:ExionLC AC
Column Name:ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
Column Temperature:50C
Flow Gradient:t=0 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=0.5 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=1 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2.1 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=5 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=7 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12.1 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=18 min: 90%/10% (%A/%B) @ 0.5 ml/min
Flow Rate:0.5 ml/min
Solvent A:100% water; 10 mM ammonium acetate
Solvent B:100% acetonitrile; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS004029
Analysis ID:AN004282
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Multiple reaction monitoring mode (MRM)
Ion Mode:NEGATIVE
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