Summary of Study ST002611

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002611
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Lung - Targeted Tricarboxylic Acid Cycle
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Mayo Clinic
DepartmentInternal Medicine
LaboratoryMayo Clinic Metabolomics Resource Core (MCMRC)
Last NameNair
First NameK. Sreekumaran
Address200 1st St SW, Rochester, MN 55902
Emailnair@mayo.edu
Phone(507) 284-2511
Submit Date2023-04-28
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailGC-MS
Release Date2023-10-06
Release Version1
K. Sreekumaran Nair K. Sreekumaran Nair
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002713
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26073290248016611Control 8 weeks of training or control time Female
SA26073390252016611Control 8 weeks of training or control time Female
SA26073490265016611Control 8 weeks of training or control time Female
SA26073590245016611Control 8 weeks of training or control time Female
SA26073690266016611Control 8 weeks of training or control time Female
SA26073790232016611Control 8 weeks of training or control time Male
SA26073890229016611Control 8 weeks of training or control time Male
SA26073990217016611Control 8 weeks of training or control time Male
SA26074090239016611Control 8 weeks of training or control time Male
SA26074190237016611Control 8 weeks of training or control time Male
SA26074290567016611Training 1 week of training or control time Female
SA26074390560016611Training 1 week of training or control time Female
SA26074490559016611Training 1 week of training or control time Female
SA26074590564016611Training 1 week of training or control time Female
SA26074690571016611Training 1 week of training or control time Female
SA26074790423016611Training 1 week of training or control time Male
SA26074890422016611Training 1 week of training or control time Male
SA26074990421016611Training 1 week of training or control time Male
SA26075090426016611Training 1 week of training or control time Male
SA26075190430016611Training 1 week of training or control time Male
SA26075290576016611Training 2 weeks of training Female
SA26075390578016611Training 2 weeks of training Female
SA26075490581016611Training 2 weeks of training Female
SA26075590585016611Training 2 weeks of training Female
SA26075690587016611Training 2 weeks of training Female
SA26075790441016611Training 2 weeks of training Male
SA26075890449016611Training 2 weeks of training Male
SA26075990450016611Training 2 weeks of training Male
SA26076090444016611Training 2 weeks of training Male
SA26076190439016611Training 2 weeks of training Male
SA26076290416016611Training 4 weeks of training Female
SA26076390412016611Training 4 weeks of training Female
SA26076490406016611Training 4 weeks of training Female
SA26076590420016611Training 4 weeks of training Female
SA26076690410016611Training 4 weeks of training Female
SA26076790283016611Training 4 weeks of training Male
SA26076890280016611Training 4 weeks of training Male
SA26076990289016611Training 4 weeks of training Male
SA26077090281016611Training 4 weeks of training Male
SA26077190292016611Training 4 weeks of training Male
SA26077290251016611Training 8 weeks of training or control time Female
SA26077390259016611Training 8 weeks of training or control time Female
SA26077490254016611Training 8 weeks of training or control time Female
SA26077590267016611Training 8 weeks of training or control time Female
SA26077690258016611Training 8 weeks of training or control time Female
SA26077790222016611Training 8 weeks of training or control time Male
SA26077890218016611Training 8 weeks of training or control time Male
SA26077990225016611Training 8 weeks of training or control time Male
SA26078090227016611Training 8 weeks of training or control time Male
SA26078190223016611Training 8 weeks of training or control time Male
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Collection:

Collection ID:CO002706
Collection Summary:-
Sample Type:Lung

Treatment:

Treatment ID:TR002723
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002719
Sampleprep Summary:homogenized in PBS, isotope IS added, deproteinized, 2 steps derivatization with ethoxamine, MTBSTFA
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004283
Analysis type MS
Chromatography type GC
Chromatography system Agilent Intuvo 9000 GC
Column Agilent DB5-MS (30m x 0.25mm, 0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5977B HES MSD
Ion Mode POSITIVE
Units nano moles per vial

Chromatography:

Chromatography ID:CH003189
Chromatography Summary:Analytes were chromatographically separated with 17 minutes GC gradient
Methods Filename:pass1b_tca_methods.pdf
Instrument Name:Agilent Intuvo 9000 GC
Column Name:Agilent DB5-MS (30m x 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS004030
Analysis ID:AN004283
Instrument Name:Agilent 5977B HES MSD
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:SIM; peak integration, concentrations calculated
Ion Mode:POSITIVE
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