Summary of Study ST002656

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002656
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Kidney - Untargeted Lipidomics, Reversed-Phase Negative
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Georgia Institute of Technology
DepartmentDepartment of Chemistry and Biochemistry
LaboratorySystems Mass Spectrometry Core (SyMSC)
Last NameFacundo
First NameFernandez
Address901 Atlantic Dr NW, Atlanta, GA 30332-0400
Emailfacundo.fernandez@chemistry.gatech.edu
Phone(404) 385-4432
Submit Date2023-05-01
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Fernandez Facundo Fernandez Facundo
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002758
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26380690248015907Control 8 weeks of training or control time Female
SA26380790266015907Control 8 weeks of training or control time Female
SA26380890265015907Control 8 weeks of training or control time Female
SA26380990245015907Control 8 weeks of training or control time Female
SA26381090252015907Control 8 weeks of training or control time Female
SA26381190229015907Control 8 weeks of training or control time Male
SA26381290217015907Control 8 weeks of training or control time Male
SA26381390237015907Control 8 weeks of training or control time Male
SA26381490239015907Control 8 weeks of training or control time Male
SA26381590232015907Control 8 weeks of training or control time Male
SA263816SampleBlank03QC-Blank 0 hour -
SA263817SampleBlank05QC-Blank 0 hour -
SA263818SampleBlank01QC-Blank 0 hour -
SA263819SampleBlank04QC-Blank 0 hour -
SA263820SampleBlank06QC-Blank 0 hour -
SA263821SampleBlank02QC-Blank 0 hour -
SA263822QC05QC-Pooled 0 hour -
SA263823QC04QC-Pooled 0 hour -
SA263824QC01QC-Pooled 0 hour -
SA263825QC03QC-Pooled 0 hour -
SA263826QC02QC-Pooled 0 hour -
SA263827QC09QC-Pooled 0 hour -
SA263828QC07QC-Pooled 0 hour -
SA263829QC06QC-Pooled 0 hour -
SA263830QC08QC-Pooled 0 hour -
SA263831SED_Reference_KidneyQC-Reference 0 hour -
SA263832IPE_Reference_Kidney02QC-Reference 0 hour -
SA263833IPE_Reference_KidneyQC-Reference 0 hour -
SA263834SED_Reference_Kidney02QC-Reference 0 hour -
SA26383590571015907Training 1 week of training or control time Female
SA26383690564015907Training 1 week of training or control time Female
SA26383790559015907Training 1 week of training or control time Female
SA26383890567015907Training 1 week of training or control time Female
SA26383990560015907Training 1 week of training or control time Female
SA26384090430015907Training 1 week of training or control time Male
SA26384190426015907Training 1 week of training or control time Male
SA26384290422015907Training 1 week of training or control time Male
SA26384390421015907Training 1 week of training or control time Male
SA26384490423015907Training 1 week of training or control time Male
SA26384590585015907Training 2 weeks of training Female
SA26384690587015907Training 2 weeks of training Female
SA26384790576015907Training 2 weeks of training Female
SA26384890578015907Training 2 weeks of training Female
SA26384990581015907Training 2 weeks of training Female
SA26385090439015907Training 2 weeks of training Male
SA26385190441015907Training 2 weeks of training Male
SA26385290444015907Training 2 weeks of training Male
SA26385390449015907Training 2 weeks of training Male
SA26385490450015907Training 2 weeks of training Male
SA26385590416015907Training 4 weeks of training Female
SA26385690412015907Training 4 weeks of training Female
SA26385790410015907Training 4 weeks of training Female
SA26385890406015907Training 4 weeks of training Female
SA26385990420015907Training 4 weeks of training Female
SA26386090283015907Training 4 weeks of training Male
SA26386190281015907Training 4 weeks of training Male
SA26386290292015907Training 4 weeks of training Male
SA26386390289015907Training 4 weeks of training Male
SA26386490280015907Training 4 weeks of training Male
SA26386590254015907Training 8 weeks of training or control time Female
SA26386690259015907Training 8 weeks of training or control time Female
SA26386790251015907Training 8 weeks of training or control time Female
SA26386890267015907Training 8 weeks of training or control time Female
SA26386990258015907Training 8 weeks of training or control time Female
SA26387090227015907Training 8 weeks of training or control time Male
SA26387190223015907Training 8 weeks of training or control time Male
SA26387290218015907Training 8 weeks of training or control time Male
SA26387390225015907Training 8 weeks of training or control time Male
SA26387490222015907Training 8 weeks of training or control time Male
Showing results 1 to 69 of 69

Collection:

Collection ID:CO002751
Collection Summary:-
Sample Type:Kidney

Treatment:

Treatment ID:TR002767
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002764
Sampleprep Summary:IPA extraction with three cycles of freeze/thaw(with sonication)
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004328
Analysis type MS
Chromatography type Reversed phase
Chromatography system Vanquish horizon LC
Column Thermo Accucore C30 (100 x 2.1mm,2.6um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode NEGATIVE
Units peak area

Chromatography:

Chromatography ID:CH003234
Chromatography Summary:global lipid separation
Methods Filename:pass1b_lrpneg_lrppos_methods.pdf
Instrument Name:Vanquish horizon LC
Column Name:Thermo Accucore C30 (100 x 2.1mm,2.6um)
Column Temperature:50C
Flow Gradient:t=0 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=1 min: 40%/60% (%A/%B) @ 0.4 ml/min | t=5 min: 30%/70% (%A/%B) @ 0.4 ml/min | t=8 min: 10%/90% (%A/%B) @ 0.4 ml/min | t=8.2 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.5 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.7 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=12 min: 80%/20% (%A/%B) @ 0.4 ml/min
Flow Rate:0.4 ml/min
Solvent A:40% water/60% acetonitrile; 10 mM ammonium formate; 0.1% formic acid
Solvent B:10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004075
Analysis ID:AN004328
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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