Summary of Study ST002658

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002658
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Kidney - Untargeted Reversed-Phase Positive
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameBurant
First NameCharles
Address6120 Brehm Tower,1000 Wall St., Ann Arbor, MI, 48105-5714
Emailburantc@med.umich.edu
Phone(734) 232-0842
Submit Date2023-05-01
Num Groups65
Total Subjects50
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Charles Burant Charles Burant
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002760
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26394490266015906Control 8 weeks of training or control time Female
SA26394590245015906Control 8 weeks of training or control time Female
SA26394690248015906Control 8 weeks of training or control time Female
SA26394790265015906Control 8 weeks of training or control time Female
SA26394890252015906Control 8 weeks of training or control time Female
SA26394990229015906Control 8 weeks of training or control time Male
SA26395090217015906Control 8 weeks of training or control time Male
SA26395190237015906Control 8 weeks of training or control time Male
SA26395290232015906Control 8 weeks of training or control time Male
SA26395390239015906Control 8 weeks of training or control time Male
SA263954CS00000SB-04QC-Blank 0 hour -
SA263955CS00000SB-06QC-Blank 0 hour -
SA263956CS00000SB-05QC-Blank 0 hour -
SA263957CS00000SB-03QC-Blank 0 hour -
SA263958CS00000PB-02QC-Blank 0 hour -
SA263959CS00000SB-02QC-Blank 0 hour -
SA263960CS00000PB-03QC-Blank 0 hour -
SA263961CS00000PB-01QC-Blank 0 hour -
SA263962CS00000SB-01QC-Blank 0 hour -
SA263963CS00000PB-04QC-Blank 0 hour -
SA263964CS00000MP-04QC-DriftCorrection 0 hour -
SA263965CS00000MP-05QC-DriftCorrection 0 hour -
SA263966CS00000MP-03QC-DriftCorrection 0 hour -
SA263967CS00000MP-06QC-DriftCorrection 0 hour -
SA263968CS00000MP-08QC-DriftCorrection 0 hour -
SA263969CS00000MP-01QC-DriftCorrection 0 hour -
SA263970CS00000MP-09QC-DriftCorrection 0 hour -
SA263971CS00000MP-02QC-DriftCorrection 0 hour -
SA263972CS00000MP-07QC-DriftCorrection 0 hour -
SA263973CS0000OP0-02QC-Pooled 0 hour -
SA263974CS0000OP0-05QC-Pooled 0 hour -
SA263975CS0000OP0-04QC-Pooled 0 hour -
SA263976CS0000OP0-03QC-Pooled 0 hour -
SA263977CS0000OP0-01QC-Pooled 0 hour -
SA263978CS0000OP0-06QC-Pooled 0 hour -
SA263979CS0000OP0-07QC-Pooled 0 hour -
SA263980CS000QCMP-03QC-Pooled 0 hour -
SA263981CS000QCMP-02QC-Pooled 0 hour -
SA263982CS000QCMP-01QC-Pooled 0 hour -
SA263983CS000QCMP-04QC-Pooled 0 hour -
SA263984CS0UMRG01-03QC-ReCAS 0 hour -
SA263985CS0UMRG01-02QC-ReCAS 0 hour -
SA263986CS0UMRG01-04QC-ReCAS 0 hour -
SA263987CS0UMRG01-01QC-ReCAS 0 hour -
SA263988CSMR80018-03QC-Reference 0 hour -
SA263989CSMR80018-02QC-Reference 0 hour -
SA263990CSMR80019-04QC-Reference 0 hour -
SA263991CSMR80018-04QC-Reference 0 hour -
SA263992CSMR80018-01QC-Reference 0 hour -
SA263993CSMR80019-02QC-Reference 0 hour -
SA263994CSMR80019-03QC-Reference 0 hour -
SA263995CSMR80019-01QC-Reference 0 hour -
SA26399690559015906Training 1 week of training or control time Female
SA26399790560015906Training 1 week of training or control time Female
SA26399890571015906Training 1 week of training or control time Female
SA26399990567015906Training 1 week of training or control time Female
SA26400090564015906Training 1 week of training or control time Female
SA26400190426015906Training 1 week of training or control time Male
SA26400290423015906Training 1 week of training or control time Male
SA26400390421015906Training 1 week of training or control time Male
SA26400490430015906Training 1 week of training or control time Male
SA26400590422015906Training 1 week of training or control time Male
SA26400690576015906Training 2 weeks of training Female
SA26400790587015906Training 2 weeks of training Female
SA26400890578015906Training 2 weeks of training Female
SA26400990585015906Training 2 weeks of training Female
SA26401090581015906Training 2 weeks of training Female
SA26401190441015906Training 2 weeks of training Male
SA26401290449015906Training 2 weeks of training Male
SA26401390444015906Training 2 weeks of training Male
SA26401490439015906Training 2 weeks of training Male
SA26401590450015906Training 2 weeks of training Male
SA26401690420015906Training 4 weeks of training Female
SA26401790406015906Training 4 weeks of training Female
SA26401890410015906Training 4 weeks of training Female
SA26401990412015906Training 4 weeks of training Female
SA26402090416015906Training 4 weeks of training Female
SA26402190280015906Training 4 weeks of training Male
SA26402290292015906Training 4 weeks of training Male
SA26402390283015906Training 4 weeks of training Male
SA26402490281015906Training 4 weeks of training Male
SA26402590289015906Training 4 weeks of training Male
SA26402690251015906Training 8 weeks of training or control time Female
SA26402790259015906Training 8 weeks of training or control time Female
SA26402890267015906Training 8 weeks of training or control time Female
SA26402990258015906Training 8 weeks of training or control time Female
SA26403090254015906Training 8 weeks of training or control time Female
SA26403190225015906Training 8 weeks of training or control time Male
SA26403290218015906Training 8 weeks of training or control time Male
SA26403390222015906Training 8 weeks of training or control time Male
SA26403490223015906Training 8 weeks of training or control time Male
SA26403590227015906Training 8 weeks of training or control time Male
Showing results 1 to 92 of 92

Collection:

Collection ID:CO002753
Collection Summary:-
Sample Type:Kidney

Treatment:

Treatment ID:TR002769
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002766
Sampleprep Summary:See SOP
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004330
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity II
Column Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6545 QTOF
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH003236
Methods Filename:pass1b_rpneg_rppos_ionpneg_methods.pdf
Instrument Name:Agilent 1290 Infinity II
Column Name:Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
Column Temperature:55C
Flow Gradient:Linear ramp from 0% to 100% B from 0-10 minutes, hold 100% B until 17 minutes, linear return to 0% B from 17 to 17.1 minutes, hold 0% B until 20 minutes
Flow Rate:0.45 ml/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.025% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004077
Analysis ID:AN004330
Instrument Name:Agilent 6545 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:See SOP protocol MiCAS_SOP004_Data post-processing and QC_v2_20190904.pdf
Ion Mode:POSITIVE
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