Summary of Study ST002694

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002694
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:White Adipose - Untargeted Reversed-Phase Positive
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameBurant
First NameCharles
Address6120 Brehm Tower,1000 Wall St., Ann Arbor, MI, 48105-5714
Emailburantc@med.umich.edu
Phone(734) 232-0842
Submit Date2023-05-02
Num Groups65
Total Subjects50
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Charles Burant Charles Burant
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002796
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26685490266017008Control 8 weeks of training or control time Female
SA26685590245017008Control 8 weeks of training or control time Female
SA26685690248017008Control 8 weeks of training or control time Female
SA26685790265017008Control 8 weeks of training or control time Female
SA26685890252017008Control 8 weeks of training or control time Female
SA26685990217017008Control 8 weeks of training or control time Male
SA26686090229017008Control 8 weeks of training or control time Male
SA26686190237017008Control 8 weeks of training or control time Male
SA26686290232017008Control 8 weeks of training or control time Male
SA26686390239017008Control 8 weeks of training or control time Male
SA266864CS00000SB-04QC-Blank 0 hour -
SA266865CS00000SB-05QC-Blank 0 hour -
SA266866CS00000SB-03QC-Blank 0 hour -
SA266867CS00000SB-02QC-Blank 0 hour -
SA266868CS00000PB-01QC-Blank 0 hour -
SA266869CS00000PB-04QC-Blank 0 hour -
SA266870CS00000PB-02QC-Blank 0 hour -
SA266871CS00000PB-03QC-Blank 0 hour -
SA266872CS00000MP-04QC-DriftCorrection 0 hour -
SA266873CS00000MP-02QC-DriftCorrection 0 hour -
SA266874CS00000MP-05QC-DriftCorrection 0 hour -
SA266875CS00000MP-03QC-DriftCorrection 0 hour -
SA266876CS00000MP-08QC-DriftCorrection 0 hour -
SA266877CS00000MP-01QC-DriftCorrection 0 hour -
SA266878CS00000MP-09QC-DriftCorrection 0 hour -
SA266879CS00000MP-07QC-DriftCorrection 0 hour -
SA266880CS00000MP-06QC-DriftCorrection 0 hour -
SA266881CS0000OP0-03QC-Pooled 0 hour -
SA266882CS0000OP0-05QC-Pooled 0 hour -
SA266883CS0000OP0-04QC-Pooled 0 hour -
SA266884CS0000OP0-02QC-Pooled 0 hour -
SA266885CS0000OP0-01QC-Pooled 0 hour -
SA266886CS0000OP0-06QC-Pooled 0 hour -
SA266887CS0000OP0-07QC-Pooled 0 hour -
SA266888CS000QCMP-03QC-Pooled 0 hour -
SA266889CS000QCMP-02QC-Pooled 0 hour -
SA266890CS000QCMP-01QC-Pooled 0 hour -
SA266891CS000QCMP-04QC-Pooled 0 hour -
SA266892CS0UMRA01-03QC-ReCAS 0 hour -
SA266893CS0UMRA01-02QC-ReCAS 0 hour -
SA266894CS0UMRA01-04QC-ReCAS 0 hour -
SA266895CS0UMRA01-01QC-ReCAS 0 hour -
SA266896CSMR80012-03QC-Reference 0 hour -
SA266897CSMR80012-02QC-Reference 0 hour -
SA266898CSMR80013-04QC-Reference 0 hour -
SA266899CSMR80012-04QC-Reference 0 hour -
SA266900CSMR80012-01QC-Reference 0 hour -
SA266901CSMR80013-03QC-Reference 0 hour -
SA266902CSMR80013-02QC-Reference 0 hour -
SA266903CSMR80013-01QC-Reference 0 hour -
SA26690490559017008Training 1 week of training or control time Female
SA26690590560017008Training 1 week of training or control time Female
SA26690690567017008Training 1 week of training or control time Female
SA26690790571017008Training 1 week of training or control time Female
SA26690890564017008Training 1 week of training or control time Female
SA26690990426017008Training 1 week of training or control time Male
SA26691090423017008Training 1 week of training or control time Male
SA26691190430017008Training 1 week of training or control time Male
SA26691290422017008Training 1 week of training or control time Male
SA26691390421017008Training 1 week of training or control time Male
SA26691490576017008Training 2 weeks of training Female
SA26691590587017008Training 2 weeks of training Female
SA26691690578017008Training 2 weeks of training Female
SA26691790585017008Training 2 weeks of training Female
SA26691890581017008Training 2 weeks of training Female
SA26691990441017008Training 2 weeks of training Male
SA26692090449017008Training 2 weeks of training Male
SA26692190444017008Training 2 weeks of training Male
SA26692290439017008Training 2 weeks of training Male
SA26692390450017008Training 2 weeks of training Male
SA26692490420017008Training 4 weeks of training Female
SA26692590406017008Training 4 weeks of training Female
SA26692690410017008Training 4 weeks of training Female
SA26692790412017008Training 4 weeks of training Female
SA26692890416017008Training 4 weeks of training Female
SA26692990280017008Training 4 weeks of training Male
SA26693090292017008Training 4 weeks of training Male
SA26693190283017008Training 4 weeks of training Male
SA26693290281017008Training 4 weeks of training Male
SA26693390289017008Training 4 weeks of training Male
SA26693490251017008Training 8 weeks of training or control time Female
SA26693590259017008Training 8 weeks of training or control time Female
SA26693690267017008Training 8 weeks of training or control time Female
SA26693790258017008Training 8 weeks of training or control time Female
SA26693890254017008Training 8 weeks of training or control time Female
SA26693990227017008Training 8 weeks of training or control time Male
SA26694090218017008Training 8 weeks of training or control time Male
SA26694190222017008Training 8 weeks of training or control time Male
SA26694290223017008Training 8 weeks of training or control time Male
SA26694390225017008Training 8 weeks of training or control time Male
Showing results 1 to 90 of 90

Collection:

Collection ID:CO002789
Collection Summary:-
Sample Type:White adipose

Treatment:

Treatment ID:TR002805
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002802
Sampleprep Summary:See SOP
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004367
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity II
Column Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6545 QTOF
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH003273
Methods Filename:pass1b_rpneg_rppos_ionpneg_methods.pdf
Instrument Name:Agilent 1290 Infinity II
Column Name:Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
Column Temperature:55C
Flow Gradient:Linear ramp from 0% to 100% B from 0-10 minutes, hold 100% B until 17 minutes, linear return to 0% B from 17 to 17.1 minutes, hold 0% B until 20 minutes
Flow Rate:0.45 ml/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.025% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004114
Analysis ID:AN004367
Instrument Name:Agilent 6545 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:See SOP protocol MiCAS_SOP004_Data post-processing and QC_v2_20190904.pdf
Ion Mode:POSITIVE
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