Summary of Study ST002699

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001671. The data can be accessed directly via it's Project DOI: 10.21228/M8Q716 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002699
Study TitleSpatially resolved metabolomics and isotope tracing reveal dynamic metabolic responses of dentate granule neurons with acute stimulation
Study SummaryKCl timecourse|Neuronal activity creates an intense energy demand that must be met by rapid metabolic responses. To define the metabolic adaptations of the neuron-enriched dentate granule cell (DGC) layer within the native tissue environment, we utilize acute hippocampal brain slices and perform fast metabolite preservation followed by mass spectrometry imaging (MALDI-MSI) to obtain spatially resolved metabolomics and isotope tracing data. We find that membrane depolarization promotes broad metabolic changes, including increased glycolytic activity in DGCs which is accompanied by mobilization of endogenous inosine into pentose phosphates.
Institute
Harvard University
Last NameYellen
First NameGary
Address220 Longwood Ave
Emailgary_yellen@hms.harvard.edu
Phone617-432-0137
Submit Date2023-04-04
Raw Data AvailableYes
Raw Data File Type(s)imzML
Analysis Type DetailMALDI-MS
Release Date2023-08-08
Release Version1
Gary Yellen Gary Yellen
https://dx.doi.org/10.21228/M8Q716
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001671
Project DOI:doi: 10.21228/M8Q716
Project Title:Spatially resolved metabolomics and isotope tracing reveal dynamic metabolic responses of dentate granule neurons with acute stimulation
Project Type:Mass spectometry imaging
Project Summary:Metabolomics of dentate gyrus in mouse hippocamal slices
Institute:Harvard University
Last Name:Yellen
First Name:Gary
Address:220 Longwood Ave
Email:gary_yellen@hms.harvard.edu
Phone:617-432-0137

Subject:

Subject ID:SU002803
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA268817Fig3 N3 10min 13C Gluc10min 13C Gluc
SA268818Fig3 N6 10min 13C Gluc10min 13C Gluc
SA268819Fig3 N2 20min 13C Gluc10min 13C Gluc
SA268820Fig3 N2 10min 13C Gluc10min 13C Gluc
SA268821Fig3 N1 10min 13C Gluc10min 13C Gluc
SA268822Fig3 N3 20min 13C Gluc10min 13C Gluc
SA268823Fig3 N1 20min 13C Gluc10min 13C Gluc
SA268824Fig3 N4 10min 13C Gluc10min 13C Gluc
SA268825Fig3 N5 20min 13C Gluc10min 13C Gluc
SA268826Fig3 N5 10min 13C Gluc10min 13C Gluc
SA268827Fig3 N6 20min 13C Gluc10min 13C Gluc
SA268828Fig3 N4 20min 13C Gluc10min 13C Gluc
SA268829Fig3 N1 10min 13C Gluc KCl10min 13C Gluc KCl
SA268830Fig3 N6 10min 13C Gluc KCl10min 13C Gluc KCl
SA268831Fig3 N5 10min 13C Gluc KCl10min 13C Gluc KCl
SA268832Fig3 N2 10min 13C Gluc KCl10min 13C Gluc KCl
SA268833Fig3 N3 10min 13C Gluc KCl10min 13C Gluc KCl
SA268834Fig3 N4 10min 13C Gluc KCl10min 13C Gluc KCl
SA268835Fig2 N7 1min KCl1min KCl
SA268836Fig2 N5 1min KCl1min KCl
SA268837Fig2 N3 1min KCl1min KCl
SA268838Fig2 N1 1min KCl1min KCl
SA268839Fig2 N4 1min KCl1min KCl
SA268840Fig2 N2 1min KCl1min KCl
SA268841Fig2 N6 1min KCl1min KCl
SA268842Fig2 N6 2min KCl2min KCl
SA268843Fig2 N7 2min KCl2min KCl
SA268844Fig2 N5 2min KCl2min KCl
SA268845Fig2 N1 2min KCl2min KCl
SA268846Fig2 N4 2min KCl2min KCl
SA268847Fig2 N2 2min KCl2min KCl
SA268848Fig2 N3 2min KCl2min KCl
SA268849Fig2 N2 30 sec KCl30 sec KCl
SA268850Fig2 N4 30 sec KCl30 sec KCl
SA268851Fig2 N5 30 sec KCl30 sec KCl
SA268852Fig2 N3 30 sec KCl30 sec KCl
SA268853Fig2 N7 30 sec KCl30 sec KCl
SA268854Fig2 N1 30 sec KCl30 sec KCl
SA268855Fig2 N6 30 sec KCl30 sec KCl
SA268856Fig3 N1 35min 13C Gluc35min 13C Gluc
SA268857Fig3 N3 35min 13C Gluc35min 13C Gluc
SA268858Fig3 N2 35min 13C Gluc35min 13C Gluc
SA268859Fig3 N4 35min 13C Gluc35min 13C Gluc
SA268860Fig3 N5 35min 13C Gluc35min 13C Gluc
SA268861Fig3 N6 35min 13C Gluc35min 13C Gluc
SA268862Fig3 N6 35min 13C Gluc KCl35min 13C Gluc KCl
SA268863Fig3 N5 35min 13C Gluc KCl35min 13C Gluc KCl
SA268864Fig3 N4 35min 13C Gluc KCl35min 13C Gluc KCl
SA268865Fig3 N2 35min 13C Gluc KCl35min 13C Gluc KCl
SA268866Fig3 N1 35min 13C Gluc KCl35min 13C Gluc KCl
SA268867Fig3 N3 35min 13C Gluc KCl35min 13C Gluc KCl
SA268868Fig2 N2 3min KCl3min KCl
SA268869Fig2 N1 3min KCl3min KCl
SA268870Fig2 N3 3min KCl3min KCl
SA268871Fig2 N7 3min KCl3min KCl
SA268872Fig2 N5 3min KCl3min KCl
SA268873Fig2 N4 3min KCl3min KCl
SA268874Fig2 N6 3min KCl3min KCl
SA268875Fig3 N1 5min 13C Gluc5min 13C Gluc
SA268876Fig3 N5 5min 13C Gluc5min 13C Gluc
SA268877Fig3 N4 5min 13C Gluc5min 13C Gluc
SA268878Fig3 N6 5min 13C Gluc5min 13C Gluc
SA268879Fig3 N3 5min 13C Gluc5min 13C Gluc
SA268880Fig3 N2 5min 13C Gluc5min 13C Gluc
SA268881Fig2 N3 5min KCl5min KCl
SA268882Fig2 N7 5min KCl5min KCl
SA268883Fig2 N5 5min KCl5min KCl
SA268884Fig2 N6 5min KCl5min KCl
SA268885Fig2 N2 5min KCl5min KCl
SA268886Fig2 N4 5min KCl5min KCl
SA268887Fig2 N6 ctrlctrl
SA268888Fig2 N1 ctrlctrl
SA268889Fig2 N4 ctrlctrl
SA268890Fig2 N3 ctrlctrl
SA268891Fig2 N5 ctrlctrl
SA268892Fig2 N2 ctrlctrl
SA268893Fig2 N7 ctrlctrl
SA268894Fig3 N1 unlabeledunlabeled
SA268895Fig3 N2 unlabeledunlabeled
SA268896Fig3 N3 unlabeledunlabeled
SA268897Fig3 N4 unlabeledunlabeled
SA268898Fig3 N5 unlabeledunlabeled
SA268899Fig3 N6 unlabeledunlabeled
Showing results 1 to 83 of 83

Collection:

Collection ID:CO002796
Collection Summary:Hippocampal slices were stimulated with 50mM potassium chloride over a time course of 5 minutes. The experiment was terminated with thermal preservation at the indicated time points.
Sample Type:Brain

Treatment:

Treatment ID:TR002812
Treatment Summary:50mM potassium chloride

Sample Preparation:

Sampleprep ID:SP002809
Sampleprep Summary:At the specific time chosen for thermal preservation, the slices were flash heated to 80°C for 2 seconds and then cooled to 4°C; the slices were then flash-frozen to -20 °C. The brain slices were stored at -80°C for subsequent thin sectioning in a cryomicrotome. The thin sections were mounted onto conductive glass slides (ITO slides) and sprayed with matrix (1,5-diaminonaphthalene).

Combined analysis:

Analysis ID AN004374
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system none
Column none
MS Type MALDI
MS instrument type QTOF
MS instrument name Bruker timsTOF fleX
Ion Mode NEGATIVE
Units a.u.

Chromatography:

Chromatography ID:CH003280
Instrument Name:none
Column Name:none
Column Temperature:none
Flow Gradient:none
Flow Rate:none
Solvent A:none
Solvent B:none
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS004123
Analysis ID:AN004374
Instrument Name:Bruker timsTOF fleX
Instrument Type:QTOF
MS Type:MALDI
MS Comments:Mass spectra were acquired on a timsTOF fleX in negative ion mode. Hippocampal sections were outlined as individual ROIs with a pixel size of 20 µm. Most metabolites were acquired in a single imaging run (m/z range 50 - 1000). Each MSI pixel consisted of 1000 laser shots. The laser Smartbeam parameter was set to single with a frequency of 10 kHz.
Ion Mode:NEGATIVE
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