Summary of Study ST002765
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001723. The data can be accessed directly via it's Project DOI: 10.21228/M80F0C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002765 |
| Study Title | Disorder of Lipids Induced by Gestational Asthma and its Effect on the Development of Fetal Lung Function and Fetal Health |
| Study Summary | Maternal asthma during pregnancy is highly correlated with fetal growth and development, and can cause damage to both the mother and fetus, but the underlying mechanisms are not yet clear. Amniotic fluid, as the environment for fetal growth and development, may be affected by lipid metabolism disorders, which can impact fetal lung function development. A rat model of asthma during pregnancy induced by common allergen house dust mite (HDM) was used to investigate changes in lipid composition in amniotic fluid and bronchoalveolar lavage fluid (BALF) by ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS), revealing the impact of maternal asthma during pregnancy on fetal lipid metabolism. In this study, maternal asthma aggravated inflammatory indicators and pathological manifestations after fetal allergen exposure, creating a high oxidative stress growth environment for the fetus, and causing metabolic differences in various lipid groups, including phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and fatty acids (FA), indicating significant lipid metabolism disorders. Improving lipid metabolism may help asthmatic pregnant women maintain healthy fetal development. |
| Institute | Nanjing University of Chinese Medicine |
| Last Name | Fang |
| First Name | Huafeng |
| Address | No.138 xianlin road, nanjing city, Nanjing, China, 210046, China |
| Riorofhf@outlook.com | |
| Phone | +86 18852416998 |
| Submit Date | 2023-06-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-01-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001723 |
| Project DOI: | doi: 10.21228/M80F0C |
| Project Title: | Lipid metabolism affects fetal development |
| Project Summary: | Gestational asthma interferes with lipidomic metabolism of amniotic fluid and fetal alveolar lavage fluid, thus inhibiting fetal development |
| Institute: | Nanjing University of Chinese Medicine |
| Last Name: | Fang |
| First Name: | Huafeng |
| Address: | No.138 xianlin road, nanjing city, Nanjing, China, 210046, China |
| Email: | Riorofhf@outlook.com |
| Phone: | +86 18852416998 |
Subject:
| Subject ID: | SU002872 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA292140 | PBS1 | Control |
| SA292141 | PBS7 | Control |
| SA292142 | PBS6 | Control |
| SA292143 | PBS2 | Control |
| SA292144 | PBS3 | Control |
| SA292145 | PBS4 | Control |
| SA292146 | HDM8 | HDM |
| SA292147 | HDM7 | HDM |
| SA292148 | HDM3 | HDM |
| SA292149 | HDM4 | HDM |
| SA292150 | HDM5 | HDM |
| SA292151 | HDM6 | HDM |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO002865 |
| Collection Summary: | Amniotic fluid was extracted from the amniotic membrane of GD18 gestational rats and stored at -80℃ |
| Sample Type: | Amniotic fluid |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002881 |
| Treatment Summary: | In the sensitization group, the sensitization solution was injected intritoneally every 2 days before pregnancy, with 100ul of sensitization solution injected each time, equivalent to 20ug of HDM injection, for a total of 3 times. The control group was given the same amount of PBS injection. Use of HDM nasal drops during pregnancy |
Sample Preparation:
| Sampleprep ID: | SP002878 |
| Sampleprep Summary: | 500 μL of amniotic fluid was lyophilized and redissolved in 200 μL of deionized water. Then, 80 μL of redissolved amniotic fluid was pipetted off into a 1.5 mL centrifuge tube containing 225 μL of ice-cooled methanol (Merck, Germany) pre-mixed with lyso PE (17:1; LM171LPE-11), SM (17:0; 170SM-13), and PE (17:0/17:0; LM170PE-19) internal standards (5 μg·mL-1; Avanti Polar Lipids, USA). The solution was vortexed for 10 s and added with 750 μL of ice-cooled MTBE. The mixture was shaken for 10 min at 4 °C, added with 188 μL of deionized water, vortexed for 20 s, and centrifuged at 18000 rpm for 2 min at 4 °C. 350 μL of the upper layer (the organic phase, mainly including lipids) and 110 μL of the bottom layer (the aqueous phase, mainly including polar substances) were separately transferred to a new centrifuge tube (1.5 mL). The samples were dried using the Savant SPD1010 vacuum centrifugal concentrator (Thermo Fisher Scientific, USA) and stored at -20 °C before testing. Lipids in the upper layers were lysed with 110 μL of methanol-toluene (9:1) solution. |
Combined analysis:
| Analysis ID | AN004500 | AN004501 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo UltiMate 3000 RS | Thermo UltiMate 3000 RS |
| Column | Waters Acquity UPLC CSH C18 column (100 mm×2.1mm, 1.7 m) | Waters Acquity UPLC CSH C18 column (100 mm×2.1mm, 1.7 m) |
| MS Type | ESI | ESI |
| MS instrument type | Other | Other |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH003381 |
| Instrument Name: | Thermo UltiMate 3000 RS |
| Column Name: | Waters Acquity UPLC CSH C18 column (100 mm×2.1mm, 1.7 m) |
| Column Temperature: | 65℃ |
| Flow Gradient: | 15% B at 0 min, 30% B at 0–2 min, 48% B at 2–2.5 min, 82% B at 2.5–11 min, 99% B at 11–12 min and 15% B at 12–15 min. |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 60％acetonitrile；40％water |
| Solvent B: | 90％isopropanol；10％ acetonitrile；0.1% formic acid；10mM ammonium formate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004247 |
| Analysis ID: | AN004500 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer was used for both positive and negative ion modes. Parameters of mass spectrometry: spray voltage was 3.5 kV (positive) and 3.0 kV (negative); for both ionization modes, sheath gas, aux gas, capillary temperature, and heater temperature were maintained at 35 arb, 15 arb, 325 °C and 300 °C, respectively; scan range was m/z 215–1800. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004248 |
| Analysis ID: | AN004501 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer was used for both positive and negative ion modes. Parameters of mass spectrometry: spray voltage was 3.5 kV (positive) and 3.0 kV (negative); for both ionization modes, sheath gas, aux gas, capillary temperature, and heater temperature were maintained at 35 arb, 15 arb, 325 °C and 300 °C, respectively; scan range was m/z 215–1800. |
| Ion Mode: | NEGATIVE |
