Summary of Study ST002769

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001726. The data can be accessed directly via it's Project DOI: 10.21228/M8M70W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002769
Study TitleThe ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (INSPIRE Cohort)
Study TypeProspective Birth Cohort Study
Study SummaryThe INSPIRE (Infant Susceptibility to Pulmonary Infections and Asthma Following RSV Exposure Study) is a population-based birth cohort of 1952 term healthy infants who will be followed to at least 11 years of age. The INSPIRE cohort was established under NIAID U19 funding in 2012 and is one of the larger ECHO cohorts. The cohort was designed such that infants were born June through December and were, on average, 6 months of age or less during their first RSV season. Biweekly surveillance was done November through April of the first year of life, with in-person respiratory illness visits conducted for those who met criteria. Viral pathogens were identified by PCR, and RSV serology was done at one year. Children are followed annually, in some years by survey, and in some years by in-person visits which include skin prick testing to aeroallergens, spirometry with test of reversibility and FeNO. Intermediate outcomes include infant respiratory morbidity, recurrent wheezing, and atopy. Asthma is defined annually using standardized questionnaires. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) Program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. INSPIRE is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
Vanderbilt University Medical Center
DepartmentVanderbilt University Medical Center, Department of Medicine, Department of Pediatrics
Last NameHartert
First NameTina
AddressCenter for Asthma Research 2525 West End Ave, Suite 450 Nashville, TN 37203
Emailtina.hartert@vumc.org
Phone615-936-1010 / 615-343-3486 (direct office)
Submit Date2023-06-29
Total Subjects630
Study CommentsHHEAR Project EM20-0011, ECHO Project EC0376
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Chear StudyYes
Analysis Type DetailLC-MS
Release Date2024-07-01
Release Version1
Tina Hartert Tina Hartert
https://dx.doi.org/10.21228/M8M70W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001726
Project DOI:doi: 10.21228/M8M70W
Project Title:The ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (INSPIRE Cohort)
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:This project was funded by the NIH Environmental influences on Child Health Outcomes (ECHO) Program. Untargeted metabolomics profiling data was acquired from urine samples provided by 14 ECHO cohorts.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner Lab
Last Name:Rushing
First Name:Blake
Address:Nutrition Research Institute , University of North Carolina at Chapel Hill, 500 Laureate Way Kannapolis, NC 28081
Email:blake_rushing@unc.edu
Phone:(704) 282-9838
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) Program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The INSPIRE ECHO Cohort is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539

Subject:

Subject ID:SU002876
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type Batch
SA294241INSPIRE_B_7blank 1
SA294242INSPIRE_B_8blank 1
SA294243INSPIRE_B_5blank 1
SA294244INSPIRE_B_6blank 1
SA294245INSPIRE_B_1blank 1
SA294246INSPIRE_B_3blank 1
SA294247INSPIRE_B_2blank 1
SA294248INSPIRE_B_4blank 1
SA294249INSPIRE_B_14blank 2
SA294250INSPIRE_B_15blank 2
SA294251INSPIRE_B_13blank 2
SA294252INSPIRE_B_12blank 2
SA294253INSPIRE_B_16blank 2
SA294254INSPIRE_B_10blank 2
SA294255INSPIRE_B_9blank 2
SA294256INSPIRE_B_11blank 2
SA294257INSPIRE_B_22blank 3
SA294258INSPIRE_B_23blank 3
SA294259INSPIRE_B_24blank 3
SA294260INSPIRE_B_21blank 3
SA294261INSPIRE_B_18blank 3
SA294262INSPIRE_B_20blank 3
SA294263INSPIRE_B_19blank 3
SA294264INSPIRE_B_17blank 3
SA294265INSPIRE_B_29blank 4
SA294266INSPIRE_B_30blank 4
SA294267INSPIRE_B_31blank 4
SA294268INSPIRE_B_28blank 4
SA294269INSPIRE_B_26blank 4
SA294270INSPIRE_B_32blank 4
SA294271INSPIRE_B_25blank 4
SA294272INSPIRE_B_27blank 4
SA294273INSPIRE_B_39blank 5
SA294274INSPIRE_B_40blank 5
SA294275INSPIRE_B_33blank 5
SA294276INSPIRE_B_37blank 5
SA294277INSPIRE_B_38blank 5
SA294278INSPIRE_B_36blank 5
SA294279INSPIRE_B_35blank 5
SA294280INSPIRE_B_34blank 5
SA294281INSPIRE_B_46blank 6
SA294282INSPIRE_B_47blank 6
SA294283INSPIRE_B_48blank 6
SA294284INSPIRE_B_45blank 6
SA294285INSPIRE_B_41blank 6
SA294286INSPIRE_B_42blank 6
SA294287INSPIRE_B_43blank 6
SA294288INSPIRE_B_44blank 6
SA294289INSPIRE_B_51blank 7
SA294290INSPIRE_B_55blank 7
SA294291INSPIRE_B_49blank 7
SA294292INSPIRE_B_54blank 7
SA294293INSPIRE_B_56blank 7
SA294294INSPIRE_B_53blank 7
SA294295INSPIRE_B_50blank 7
SA294296INSPIRE_B_52blank 7
SA294297INSPIRE_B_62blank 8
SA294298INSPIRE_B_63blank 8
SA294299INSPIRE_B_61blank 8
SA294300INSPIRE_B_58blank 8
SA294301INSPIRE_B_57blank 8
SA294302INSPIRE_B_59blank 8
SA294303INSPIRE_B_60blank 8
SA293428INSPIRE_CHEAR_U_1CHEAR 1
SA293429INSPIRE_CHEAR_U_4CHEAR 1
SA293430INSPIRE_CHEAR_U_2CHEAR 1
SA293431INSPIRE_CHEAR_U_3CHEAR 1
SA293432INSPIRE_CHEAR_U_8CHEAR 2
SA293433INSPIRE_CHEAR_U_7CHEAR 2
SA293434INSPIRE_CHEAR_U_5CHEAR 2
SA293435INSPIRE_CHEAR_U_6CHEAR 2
SA293436INSPIRE_CHEAR_U_12CHEAR 3
SA293437INSPIRE_CHEAR_U_11CHEAR 3
SA293438INSPIRE_CHEAR_U_10CHEAR 3
SA293439INSPIRE_CHEAR_U_9CHEAR 3
SA293440INSPIRE_CHEAR_U_16CHEAR 4
SA293441INSPIRE_CHEAR_U_15CHEAR 4
SA293442INSPIRE_CHEAR_U_13CHEAR 4
SA293443INSPIRE_CHEAR_U_14CHEAR 4
SA293444INSPIRE_CHEAR_U_20CHEAR 5
SA293445INSPIRE_CHEAR_U_19CHEAR 5
SA293446INSPIRE_CHEAR_U_17CHEAR 5
SA293447INSPIRE_CHEAR_U_18CHEAR 5
SA293448INSPIRE_CHEAR_U_24CHEAR 6
SA293449INSPIRE_CHEAR_U_23CHEAR 6
SA293450INSPIRE_CHEAR_U_21CHEAR 6
SA293451INSPIRE_CHEAR_U_22CHEAR 6
SA293452INSPIRE_CHEAR_U_25CHEAR 7
SA293453INSPIRE_CHEAR_U_26CHEAR 7
SA293454INSPIRE_CHEAR_U_28CHEAR 7
SA293455INSPIRE_CHEAR_U_27CHEAR 7
SA293456INSPIRE_CHEAR_U_31CHEAR 8
SA293457INSPIRE_CHEAR_U_32CHEAR 8
SA293458INSPIRE_CHEAR_U_30CHEAR 8
SA293459INSPIRE_CHEAR_U_29CHEAR 8
SA293460INSPIRE_HHEAR_U1_3HHEAR U1 1
SA293461INSPIRE_HHEAR_U1_4HHEAR U1 1
SA293462INSPIRE_HHEAR_U1_2HHEAR U1 1
SA293463INSPIRE_HHEAR_U1_1HHEAR U1 1
SA293464INSPIRE_HHEAR_U1_8HHEAR U1 2
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Collection:

Collection ID:CO002869
Collection Summary:N/A
Sample Type:Urine
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002885
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002882
Sampleprep Summary:Study samples were shipped from the Vanderbilt University Medical Center (Department of Medicine, Department of Pediatrics) to the NC HHEAR Hub on dry ice. The NC HHEAR Hub thawed and transferred 50 µL of the study samples to a new set of tubes and used them for the analysis. An additional 10 µL was taken from the original study sample and transferred to another tube to make the total study pool for this project, and then distributed with 50 µL per aliquot, used as quality control study pools (QC study pools) throughout the whole analysis. All sample aliquots (50 µL each) and QC study pools (50 µL each) were stored at -80° C until the day of sample preparation. HHEAR Urine Pool 1 (50 µL each), HHEAR Urine Pool 2 (50 µL each), CHEAR reference urine (50 µL each) and NIST urine (SRM 3672) reference material (50 µL each) were provided by the NC HHEAR Hub. LC-MS grade water (50 µL) was used as blanks. All samples were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference urine, CHEAR reference urine, NIST reference urine, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:4℃
Extraction Method:Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard
Extract Storage:-80℃
Sample Resuspension:Water-Methanol (95:5, v/v)
Sample Spiking:Tryptophan-d5 stock solution at 500 ng/mL

Combined analysis:

Analysis ID AN004506
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Scientific Vanquish UPHPLC
Column Acquity UPLC HSS T3 C18 (100 X 2.1mm, 1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units Normalized Intensities

Chromatography:

Chromatography ID:CH003386
Chromatography Summary:Reversed phase
Instrument Name:Thermo Scientific Vanquish UPHPLC
Column Name:Acquity UPLC HSS T3 C18 (100 X 2.1mm, 1.8um)
Column Pressure:6000-10000 psi
Column Temperature:50 ℃
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 mL/min
Injection Temperature:8 ℃
Internal Standard:Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22 min
Capillary Voltage:3.5 KV
Weak Wash Solvent Name:10% methanol/90% water; 0.1% formic acid
Strong Wash Solvent Name:75% 2-Propanol/25% Water; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004253
Analysis ID:AN004506
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 KV
Collision Energy:20-45, ramp
Collision Gas:N2
Fragmentation Method:CID
Ion Spray Voltage:3.5kV
Ionization:ES+
Mass Accuracy:5 ppm
Dataformat:Profile
Desolvation Gas Flow:55
Desolvation Temperature:400 ℃
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