Summary of Study ST002781

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001715. The data can be accessed directly via it's Project DOI: 10.21228/M81D9R This work is supported by NIH grant, U2C- DK119886.

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Study IDST002781
Study TitleLipidomics analysis of maternal obesity model - wild type
Study Summary10μl liver tissue of 11-12 weeks old male mice from various diet groups with various genetic background were homogenized and lipids were extracted for shotgun lipidomics experiments. Raw files were converted to .mzml files and imported into and analyzed by LipidXplorer software using custom mfql files to identify sample lipids and internal standards. For further data processing, absolute amounts were calculated using the internal standard intensities followed by the calculated mol% of the identified lipids. For wild-type C57BL/6JRcc mice, diet groups are CDm CDl CD, CDm CDl HFD, CDm HFDl HFD, HFDm CDl CD, HFDm CDl HFD, HFDm HFDl CD and HFDm CDl CD. m: maternal diet, l: lactation phase diet.
Institute
University of Bonn
DepartmentLIMES
LaboratoryMass Lab
Last NameMass
First NameElvira
AddressCarl-Troll-Str. 31, 53115 Bonn, Germany
Emailelvira.mass@uni-bonn.de
Phone+49 0228 / 73 6 28 48
Submit Date2023-07-02
Num Groups6
Total Subjects28
Analysis Type DetailMS(Dir. Inf.)
Release Date2023-08-01
Release Version1
Elvira Mass Elvira Mass
https://dx.doi.org/10.21228/M81D9R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001715
Project DOI:doi: 10.21228/M81D9R
Project Title:Developmental programming of Kupffer cells by maternal obesity causes fatty liver disease in the offspring
Project Summary:Kupffer cells (KCs) are tissue-resident macrophages which colonize the developing liver early during embryogenesis. Throughout development and adulthood, KCs have distinct core functions that are essential for liver and organismal homeostasis, such as supporting fetal erythropoiesis as well as postnatal erythrocyte recycling and liver metabolism. KCs acquire their tissue-specific transcriptional signature immediately after colonizing the liver, mature together with the tissue, and adapt to the tissue’s function. However, whether perturbation of macrophage core functions during development may contribute to or cause disease at postnatal stages is poorly understood. Here, we utilize a maternal obesity model to disturb KC functions during gestation. We show that offspring born to obese mothers develop fatty liver disease that is accompanied by a local pro-inflammatory response, a phenotype that is augmented if the offspring is kept on control diet after birth. Further, transcriptional analyses reveal that KCs undergo developmental programming through the maternal high-fat diet, which lasts until adulthood. The offspring’s KC developmental programming is irreversible despite the switch to control diet and leads to increased lipid uptake in hepatocytes mediated via paracrine factors stemming from KCs. The transcriptional programming of KCs and the fatty liver disease phenotype are rescued by genetic depletion of hypoxia-inducible factor alpha (Hif-1alpha) in macrophages during gestation. These results demonstrate that macrophages rely on an undisturbed development to fulfil their core functions and support organ homeostasis during adulthood, and establish developmental programming of KCs as a therapeutic strategy for metabolic disorders, such as fatty liver disease.
Institute:University of Bonn
Department:LIMES
Laboratory:Mass Lab
Last Name:Mass
First Name:Elvira
Address:Carl-Troll-Str. 31, 53115 Bonn, Germany
Email:elvira.mass@uni-bonn.de
Phone:+49 0228 / 73 6 28 48
Funding Source:DFG
Publications:in preparation
Contributors:Nora Balzer, Iva Splichalova, Hao Huang, Stephan Grein, Lea Seep

Subject:

Subject ID:SU002888
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6JRcc
Age Or Age Range:10-12 weeks
Animal Housing:specific pathogen-free conditions
Animal Light Cycle:12-h light/dark cycle

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id diet
SA298596CD_CD_CD_103CD_CD_CD
SA298597CD_CD_CD_106CD_CD_CD
SA298598CD_CD_CD_105CD_CD_CD
SA298599CD_CD_HFD_207CD_CD_HFD
SA298600CD_CD_HFD_205CD_CD_HFD
SA298601CD_CD_HFD_206CD_CD_HFD
SA298602CD_CD_HFD_201CD_CD_HFD
SA298603CD_CD_HFD_200CD_CD_HFD
SA298604CD_CD_HFD_203CD_CD_HFD
SA298605HFD_CD_CD_803HFD_CD_CD
SA298606HFD_CD_CD_801HFD_CD_CD
SA298607HFD_CD_CD_802HFD_CD_CD
SA298608HFD_CD_CD_800HFD_CD_CD
SA298609HFD_CD_HFD_703HFD_CD_HFD
SA298610HFD_CD_HFD_701HFD_CD_HFD
SA298611HFD_CD_HFD_702HFD_CD_HFD
SA298612HFD_CD_HFD_700HFD_CD_HFD
SA298613HFD_HFD_CD_605HFD_HFD_CD
SA298614HFD_HFD_CD_604HFD_HFD_CD
SA298615HFD_HFD_CD_606HFD_HFD_CD
SA298616HFD_HFD_CD_603HFD_HFD_CD
SA298617HFD_HFD_CD_601HFD_HFD_CD
SA298618HFD_HFD_CD_602HFD_HFD_CD
SA298619HFD_HFD_HFD_504HFD_HFD_HFD
SA298620HFD_HFD_HFD_503HFD_HFD_HFD
SA298621HFD_HFD_HFD_501HFD_HFD_HFD
SA298622HFD_HFD_HFD_500HFD_HFD_HFD
SA298623HFD_HFD_HFD_502HFD_HFD_HFD
Showing results 1 to 28 of 28

Collection:

Collection ID:CO002881
Collection Summary:Livers were isolated from PBS-perfused mice, and 10 µg was homogenized in ddH2O using a Precellys homogenizer (Peqlab Biotechnology). The mice were subjected different diet schemes.
Collection Protocol Filename:Lipidomics_Notes.pdf
Sample Type:Liver

Treatment:

Treatment ID:TR002897
Treatment Summary:Samples were taken from the developed Maternal Obesity model. This model was generated as described in the Material and Methods section. No additional treatment was done.

Sample Preparation:

Sampleprep ID:SP002894
Sampleprep Summary:In this experiment, mouse livers were isolated and processed to extract lipids for analysis. The livers were homogenized and subjected to a series of centrifugation and phase separation steps to obtain the lipid samples. The extracted lipids were then prepared for further analysis by adding specific internal standards and a spray buffer, allowing for subsequent characterization and investigation.
Sampleprep Protocol Filename:Lipidomics_Notes.pdf

Combined analysis:

Analysis ID AN004528
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system Thermo Q Exactive Plus
Column none
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE
Units pmol/mg

Chromatography:

Chromatography ID:CH003401
Instrument Name:Thermo Q Exactive Plus
Column Name:none
Column Temperature:none
Flow Gradient:-
Flow Rate:10 µl/min
Injection Temperature:260
Solvent A:8/5/1 isopropanol/methanol/water; 10 mM ammonium acetate
Solvent B:-
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS004275
Analysis ID:AN004528
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Thermo Q Exactive Plus spectrometer equipped with the HESI II ion source. MS1 spectra (resolution 280 000) were recorded in 100 m/z windows from 250 to 1200 m/z (pos.) and 200 - 1700 m/z (neg.) followed by recording MS/MS spectra (res. 70 000) by data-independent acquisition in 1 m/z windows from 200 to 1200 (pos.) and 200 to 1700 (neg.) m/z. Raw files were converted to .mzml files and imported into and analyzed by LipidXplorer software using custom mfql files to identify sample lipids and internal standards. For further data processing, absolute amounts were calculated using the internal standard intensities followed by the calculated mol% of the identified lipids.
Ion Mode:POSITIVE
Analysis Protocol File:Lipidomics_Notes.pdf
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