Summary of Study ST002802
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001749. The data can be accessed directly via it's Project DOI: 10.21228/M8N14N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002802 |
| Study Title | Nontargeted Serum Metabolomic Profiling of FXR-null and Wild-type Mice |
| Study Summary | This study analyzed the serum metabolome of 10 FXR-knockout and 10 wild-type mice by RPLC-HRMS. |
| Institute | University of North Carolina at Chapel Hill |
| Department | Department of Environmental Sciences and Engineering |
| Laboratory | Kun Lu |
| Last Name | Hsiao |
| First Name | Yun-Chung |
| Address | 135 Dauer Dr. MHRC1104 |
| ychsiao@live.unc.edu | |
| Phone | 9842159592 |
| Submit Date | 2023-07-31 |
| Num Groups | 2 |
| Total Subjects | 20 |
| Num Males | 20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-08-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001749 |
| Project DOI: | doi: 10.21228/M8N14N |
| Project Title: | Nontargeted Serum Metabolomic Profiling of FXR-null and Wild-type Mice |
| Project Summary: | This study analyzed the serum metabolome of 10 FXR-knockout and 10 wild-type mice by RPLC-HRMS. |
| Institute: | University of North Carolina at Chapel Hill |
| Department: | Department of Environmental Sciences and Engineering |
| Laboratory: | Kun Lu |
| Last Name: | Hsiao |
| First Name: | Yun-Chung |
| Address: | 135 Dauer Dr. MHRC1104 |
| Email: | ychsiao@live.unc.edu |
| Phone: | 9842159592 |
| Funding Source: | The research was supported by the UNC Superfund Research program (P42ES031007), University of North Carolina Center for Environmental Health and Susceptibility grant (P30ES010126). |
Subject:
| Subject ID: | SU002909 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 6 months |
| Weight Or Weight Range: | 27-43 |
| Gender: | Male |
| Animal Animal Supplier: | The Jackson Laboratory |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA300792 | G32-Con-32A5A | FXR-knockout |
| SA300793 | G32-Con-32A2B | FXR-knockout |
| SA300794 | G32-Con-32A2A | FXR-knockout |
| SA300795 | G32-Con-32A5B | FXR-knockout |
| SA300796 | G32-Con-32A6B | FXR-knockout |
| SA300797 | G32-Con-32A8B | FXR-knockout |
| SA300798 | G32-Con-32A8A | FXR-knockout |
| SA300799 | G32-Con-32A1B | FXR-knockout |
| SA300800 | G32-Con-32A6A | FXR-knockout |
| SA300801 | G32-Con-32A1A | FXR-knockout |
| SA300802 | G31-Con-31A3A | Wild-type |
| SA300803 | G31-Con-31A2B | Wild-type |
| SA300804 | G31-Con-31A2A | Wild-type |
| SA300805 | G31-Con-31A1B | Wild-type |
| SA300806 | G31-Con-31A3B | Wild-type |
| SA300807 | G31-Con-31A4A | Wild-type |
| SA300808 | G31-Con-31A5B | Wild-type |
| SA300809 | G31-Con-31A5A | Wild-type |
| SA300810 | G31-Con-31A4B | Wild-type |
| SA300811 | G31-Con-31A1A | Wild-type |
| Showing results 1 to 20 of 20 |
Collection:
| Collection ID: | CO002902 |
| Collection Summary: | Serum samples from each mouse were collected from heart blood immediately after carbon dioxide euthanasia. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR002918 |
| Treatment Summary: | No treatment. |
Sample Preparation:
| Sampleprep ID: | SP002915 |
| Sampleprep Summary: | Serum samples from each mouse were thawed and 20 μL was consumed to extract metabolites for each mice. Metabolite extraction was facilitated by adding 180 μL methanol containing stable isotope-labeled chemicals ([D5]-glutamine, [D2]-𝛾-aminobutyric acid, [D3]-tryptophan, [D2]-indole-3-propionic acid, [D2]-indole-3-acetic acid, [D13]-acetylcholine in 500 nM; [D4]-serotonin in 250 nM; and [D4]-kynurenic acid in 50 nM), and incubated under -20°C for 1 hr, followed by centrifugation under 15,000 ×g, 4°C for 10 minutes to collect the supernatant (150 μL). The supernatant was dried by SpeedVac® and reconstituted with 100 μL 2% acetonitrile in water. |
Chromatography:
| Chromatography ID: | CH003425 |
| Chromatography Summary: | The serum analytes were injected (10 μL) into a Waters Acquity UPLC HSS T3 (reverse phase C18, 100 Å, 1.8 μm, 2.1 mm × 100 mm) analytical column controlled at 40 °C, with the mobile phase composed of water (A) and acetonitrile (B) both added with 0.1% formic acid at a flow rate of 0.4 mL/min. The 15-min-gradient for chromatographic separation was set as the following: 2% B from 0-1 min; 2%-15% B from 1-3 min; 15%-50% B from 3-6 min; 50%-98% B from 6-7.5 min; 98% B held from 7.5-11.5 min; 98%-2% B from 11.5-11.6 min; and 2% B held from 11.6-15 min for a final re-equilibration. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Gradient: | 2% B from 0-1 min; 2%-15% B from 1-3 min; 15%-50% B from 3-6 min; 50%-98% B from 6-7.5 min; 98% B held from 7.5-11.5 min; 98%-2% B from 11.5-11.6 min; and 2% B held from 11.6-15 min for a final re-equilibration. |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN004558 |
| Analysis Type: | MS |
| Chromatography ID: | CH003425 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST002802_AN004558_Results.txt |
| Units: | m/z |
