Summary of Study ST002918

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001799. The data can be accessed directly via it's Project DOI: 10.21228/M85M79 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST002918
Study Title	Metabolic Profiling in mouse Infected with Vibrio parahaemolyticus
Study Summary	Mice were subject to intraperitoneal (i.p.) injection with the V. parahaemolyticus, and control mice were injected with saline. Data were collected and analyzed using unsupervised hierarchical clustering. In general, the abundance of metabolites increased more often than it decreased in animals with higher resistance to infection. Load weight analysis of biological pathways suggested that alanine, aspartate, glutamate metabolism could play roles in the capacity of mice to survive infection with V. parahaemolyticus.
Institute	Sun Yat-sen University
Last Name	jiang
First Name	ming
Address	No. 135, Xingang Xi Road, Guangzhou, 510275, P. R. China
Email	jiangm28@mail.sysu.edu.cn
Phone	13434283781
Submit Date	2023-09-16
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	GC-MS
Release Date	2023-10-05
Release Version	1

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Project:

Project ID:	PR001799
Project DOI:	doi: 10.21228/M85M79
Project Title:	Exogenous L-Alanine promotes phagocytosis via dual regulations of TLR4 to eliminate multidrug-resistant bacterial pathogens
Project Type:	MS quantitative analysis
Project Summary:	Multidrug-resistant bacteria present a major threat to public health. Therefore, new drugs or approaches are urgently needed to manage and mitigate this threat. Here, we screen the molecular candidates that allow the survival of mice upon multidrug-resistant Vibrio parahaemolyticus infection by integrated proteomic and metabolomics analysis, where L-Alanine metabolism and phagocytosis are highly correlated. The role of L-Alanine on boosting mouse survival is further confirmed with in vivo bacterial challenge studies on multidrug-resistant bacteria including V. parahaemolyticus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae. Functional studies demonstrate that exogenous L-Alanine promotes phagocytosis to these different species of multidrug-resistant pathogens. The underlying mechanism involves two events that are L-Alanine-dependently increased TLR4 expression, and L-Alanine-enhanced TLR4 signaling via increasing the biosynthesis and secretion of fatty acids such as palmitate. Palmitate enhances the binding of LPS to TLR4 and thereby promotes TLR4 dimmer formation and endocytosis for the subsequent activation of PI3K/Akt and NF-κB pathways and phagocytosis of bacteria. These results suggest that modulation of metabolic environment is a plausible approach for combating infection with multidrug-resistant bacteria.
Institute:	sun yat-sen university
Last Name:	jiang
First Name:	ming
Address:	No. 135, Xingang Xi Road, Guangzhou, 510275, P. R. China, guangzhou, guangdong, 510006, China
Email:	jiangm28@mail.sysu.edu.cn
Phone:	13434283781

Subject:

Subject ID:	SU003031
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	Treatment
SA316666	20120522-zhxl-test-S1-1	control
SA316667	20120522-zhxl-test-S3-1	control
SA316668	20120522-zhxl-test-S3-2	control
SA316669	20120522-zhxl-test-S2-2	control
SA316670	20120522-zhxl-test-S1-2	control
SA316671	20120522-zhxl-test-S2-1	control
SA316672	20120522-zhxl-C9-2	infection
SA316673	20120522-zhxl-C10-1	infection
SA316674	20120522-zhxl-C8-2	infection
SA316675	20120522-zhxl-C10-2	infection
SA316676	20120522-zhxl-C9-1	infection
SA316677	20120522-zhxl-A2-L-1	infection
SA316678	20120522-zhxl-A2-L-2	infection
SA316679	20120522-zhxl-C8-1	infection
SA316680	20120522-zhxl-A1-L-2	infection
SA316681	20120522-zhxl-A1-L-1	infection
SA316682	20120522-zhxl-C6-1	infection
SA316683	20120522-zhxl-C4-2	infection
SA316684	20120522-zhxl-C4-1	infection
SA316685	20120522-zhxl-C3-2	infection
SA316686	20120522-zhxl-C5-1	infection
SA316687	20120522-zhxl-C5-2	infection
SA316688	20120522-zhxl-C7-1	infection
SA316689	20120522-zhxl-C6-2	infection
SA316690	20120522-zhxl-A3-L-1	infection
SA316691	20120522-zhxl-C7-2	infection
SA316692	20120522-zhxl-A5-L-1	infection
SA316693	20120522-zhxl-C2-L-1	infection
SA316694	20120522-zhxl-C1-L-2	infection
SA316695	20120522-zhxl-C1-L-1	infection
SA316696	20120522-zhxl-B3-L-2	infection
SA316697	20120522-zhxl-C2-L-2	infection
SA316698	20120522-zhxl-C3-L-1	infection
SA316699	20120522-zhxl-C4-L-2	infection
SA316700	20120522-zhxl-C4-L-1	infection
SA316701	20120522-zhxl-C3-L-2	infection
SA316702	20120522-zhxl-B3-L-1	infection
SA316703	20120522-zhxl-B2-L-2	infection
SA316704	20120522-zhxl-A5-L-2	infection
SA316705	20120522-zhxl-C3-1	infection
SA316706	20120522-zhxl-A4-L-2	infection
SA316707	20120522-zhxl-A4-L-1	infection
SA316708	20120522-zhxl-A6-L-1	infection
SA316709	20120522-zhxl-A6-L-2	infection
SA316710	20120522-zhxl-B2-L-1	infection
SA316711	20120522-zhxl-B1-L-2	infection
SA316712	20120522-zhxl-B1-L-1	infection
SA316713	20120522-zhxl-A3-L-2	infection
SA316714	20120522-zhxl-C1-1	infection
SA316715	20120522-zhxl-A8-1	infection
SA316716	20120522-zhxl-A7-2	infection
SA316717	20120522-zhxl-A7-1	infection
SA316718	20120522-zhxl-A6-2	infection
SA316719	20120522-zhxl-A8-2	infection
SA316720	20120522-zhxl-A9-1	infection
SA316721	20120522-zhxl-A10-2	infection
SA316722	20120522-zhxl-A10-1	infection
SA316723	20120522-zhxl-A9-2	infection
SA316724	20120522-zhxl-A6-1	infection
SA316725	20120522-zhxl-A5-2	infection
SA316726	20120522-zhxl-A2-2	infection
SA316727	20120522-zhxl-A2-1	infection
SA316728	20120522-zhxl-A1-2	infection
SA316729	20120522-zhxl-A1-1	infection
SA316730	20120522-zhxl-A3-1	infection
SA316731	20120522-zhxl-A3-2	infection
SA316732	20120522-zhxl-A5-1	infection
SA316733	20120522-zhxl-A4-2	infection
SA316734	20120522-zhxl-A4-1	infection
SA316735	20120522-zhxl-B1-1	infection
SA316736	20120522-zhxl-B1-2	infection
SA316737	20120522-zhxl-B9-1	infection
SA316738	20120522-zhxl-B8-2	infection
SA316739	20120522-zhxl-B8-1	infection
SA316740	20120522-zhxl-B7-2	infection
SA316741	20120522-zhxl-B9-2	infection
SA316742	20120522-zhxl-B10-1	infection
SA316743	20120522-zhxl-C2-1	infection
SA316744	20120522-zhxl-C1-2	infection
SA316745	20120522-zhxl-B10-2	infection
SA316746	20120522-zhxl-B7-1	infection
SA316747	20120522-zhxl-B6-2	infection
SA316748	20120522-zhxl-B3-2	infection
SA316749	20120522-zhxl-B3-1	infection
SA316750	20120522-zhxl-B2-2	infection
SA316751	20120522-zhxl-B2-1	infection
SA316752	20120522-zhxl-B4-1	infection
SA316753	20120522-zhxl-B4-2	infection
SA316754	20120522-zhxl-B6-1	infection
SA316755	20120522-zhxl-B5-2	infection
SA316756	20120522-zhxl-B5-1	infection
SA316757	20120522-zhxl-C2-2	infection

Showing results 1 to 92 of 92

Showing results 1 to 92 of 92

Collection:

Collection ID:	CO003024
Collection Summary:	Samples were counted, washed with cold PBS and then flash-frozen in liquid N2
Sample Type:	Spleen

Treatment:

Treatment ID:	TR003040
Treatment Summary:	Spleen tissues were weighed and homogenized with the first solvent (the mixture of chloroform, methanol and water (1:2:1, v/v/v)) for 30 s at 4 0C and then centrifuged at 12,000 rpm for 10 min at 4 0C. The supernatant was collected and deposit was re-homogenized with the second solvent (methanol alone) before a second centrifugation. The two supernatants were mixed, and aliquot of sample was transferred to a GC sampling vial containing 5 μL 0.1 mg/mL ribitol (Sigma) as an analytical internal standard and then dried in a vacuum centrifuge concentrator before the subsequent derivatization. Two technical replicates were prepared for each sample.

Treatment ID:

TR003040

Treatment Summary:

Spleen tissues were weighed and homogenized with the first solvent (the mixture of chloroform, methanol and water (1:2:1, v/v/v)) for 30 s at 4 0C and then centrifuged at 12,000 rpm for 10 min at 4 0C. The supernatant was collected and deposit was re-homogenized with the second solvent (methanol alone) before a second centrifugation. The two supernatants were mixed, and aliquot of sample was transferred to a GC sampling vial containing 5 μL 0.1 mg/mL ribitol (Sigma) as an analytical internal standard and then dried in a vacuum centrifuge concentrator before the subsequent derivatization. Two technical replicates were prepared for each sample.

Sample Preparation:

Sampleprep ID:	SP003037
Sampleprep Summary:	Spleen tissues were weighed and homogenized with the first solvent (the mixture of chloroform, methanol and water (1:2:1, v/v/v)) for 30 s at 4 0C and then centrifuged at 12,000 rpm for 10 min at 4 0C. The supernatant was collected and deposit was re-homogenized with the second solvent (methanol alone) before a second centrifugation. The two supernatants were mixed, and aliquot of sample was transferred to a GC sampling vial containing 5 μL 0.1 mg/mL ribitol (Sigma) as an analytical internal standard and then dried in a vacuum centrifuge concentrator before the subsequent derivatization. Two technical replicates were prepared for each sample.

Sampleprep ID:

SP003037

Sampleprep Summary:

Combined analysis:

Analysis ID	AN004788
Analysis type	MS
Chromatography type	GC
Chromatography system	Thermo Scientific Trace GC Ultra with DSQ II GC/MS
Column	Agilent DB5-MS (30m x 0.25mm, 0.25um)
MS Type	EI
MS instrument type	Triple quadrupole
MS instrument name	Thermo Scientific Trace GC Ultra with DSQ II GC/MS
Ion Mode	POSITIVE
Units	Peak area

Chromatography:

Chromatography ID:	CH003619
Chromatography Summary:	Low pH polar (LC/MS Pos early)
Instrument Name:	Thermo Scientific Trace GC Ultra with DSQ II GC/MS
Column Name:	Agilent DB5-MS (30m x 0.25mm, 0.25um)
Column Temperature:	270 °C
Flow Gradient:	none
Flow Rate:	1.0 mL/min
Solvent A:	none
Solvent B:	none
Chromatography Type:	GC

MS:

MS ID:	MS004534
Analysis ID:	AN004788
Instrument Name:	Thermo Scientific Trace GC Ultra with DSQ II GC/MS
Instrument Type:	Triple quadrupole
MS Type:	EI
MS Comments:	samples was derivatized and then used to firstly protect carbonyl moieties through methoximation, through a 90 min 37 ℃ reaction with 40 μL of 20 mg/mL methoxyamine hydrochloride (Sigma-Aldrich) in pyridine, followed by derivatization of acidic protons through a 30 min 37 0C reaction with the addition of 80 μL N-methyl-N-trimethylsilyltrifluoroace-tamide (MSTFA, Sigma-Aldrich). The derivatized sample of 1 μL was injected into a 30m × 250 μm i.d. × 0.25 μm DBS-MS column using splitless injection and analysis was carried out by Trace DSQ II (Thermo Scientific). The initial temperature of the GC oven was held at 85 0C for 5 min followed by an increase to 330 0C at a rate of 15 0C min-1 then held for 5 min. Helium was used as carrier gas and flow was kept constant at 1 mL min-1. The MS was operated in a range of 50-600 m/z.
Ion Mode:	POSITIVE