Summary of Study ST002981

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001857. The data can be accessed directly via it's Project DOI: 10.21228/M8P99M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST002981
Study Title	Developmental Neurotoxicity of Deltamethrin Exposure on Hypothalamic Neurogenesis in Embryonic Zebrafish
Study Summary	In this study, to investigate DM effects at different stages of early life, zebrafish embryos were exposed to DM just before (10-16 hpf), at the onset of (16-24 hpf), at the peak of (24-36 hpf) hypothalamic neurogenesis and across 10-120 hpf with different dosage levels (0, 1, 100, and 250 nM).
Institute	College of Marine Food and Biological Engineering, Jimei University
Last Name	Gao
First Name	Longhua
Address	No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, China
Email	1932076629@qq.com
Phone	18718180398
Submit Date	2023-11-12
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2023-11-22
Release Version	1

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Project:

Project ID:	PR001857
Project DOI:	doi: 10.21228/M8P99M
Project Title:	Developmental Neurotoxicity of Deltamethrin Exposure on Hypothalamic Neurogenesis in Embryonic Zebrafish
Project Summary:	In this study, to investigate DM effects at different stages of early life, zebrafish embryos were exposed to DM just before (10-16 hpf), at the onset of (16-24 hpf), at the peak of (24-36 hpf) hypothalamic neurogenesis and across 10-120 hpf with different dosage levels (0, 1, 100, and 250 nM).
Institute:	College of Marine Food and Biological Engineering, Jimei University
Last Name:	Gao
First Name:	Longhua
Address:	No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, China
Email:	1932076629@qq.com
Phone:	18718180398

Subject:

Subject ID:	SU003094
Subject Type:	Fish
Subject Species:	Danio rerio
Taxonomy ID:	7955

Factors:

Subject type: Fish; Subject species: Danio rerio (Factor headings shown in green)

mb_sample_id	local_sample_id	Treatment
SA323431	100n-16-24_2	100nM
SA323432	100n-16-24_3	100nM
SA323433	100n-16-24_6	100nM
SA323434	100n-16-24_1	100nM
SA323435	100n-16-24_5	100nM
SA323436	100n-10-16_5	100nM
SA323437	100n-10-16_2	100nM
SA323438	100n-10-16_3	100nM
SA323439	100n-10-16_4	100nM
SA323440	100n-24-36_1	100nM
SA323441	100n-10-16_6	100nM
SA323442	100n-24-36_3	100nM
SA323443	100n-10-5d_3	100nM
SA323444	100n-10-5d_4	100nM
SA323445	100n-10-5d_5	100nM
SA323446	100n-10-5d_6	100nM
SA323447	100n-10-5d_2	100nM
SA323448	100n-10-5d_1	100nM
SA323449	100n-10-16_1	100nM
SA323450	100n-24-36_4	100nM
SA323451	100n-24-36_5	100nM
SA323452	100n-24-36_6	100nM
SA323453	100n-24-36_2	100nM
SA323454	100n-16-24_4	100nM
SA323455	1n-16-24_2	1nM
SA323456	1n-16-24_1	1nM
SA323457	1n-16-24_3	1nM
SA323458	1n-16-24_4	1nM
SA323459	1n-16-24_5	1nM
SA323460	1n-10-16_6	1nM
SA323461	1n-10-16_5	1nM
SA323462	1n-10-5d_2	1nM
SA323463	1n-10-16_2	1nM
SA323464	1n-10-16_3	1nM
SA323465	1n-10-16_4	1nM
SA323466	1n-16-24_6	1nM
SA323467	1n-10-16_1	1nM
SA323468	1n-10-5d_6	1nM
SA323469	1n-24-36_1	1nM
SA323470	1n-10-5d_5	1nM
SA323471	1n-10-5d_4	1nM
SA323472	1n-10-5d_3	1nM
SA323473	1n-24-36_6	1nM
SA323474	1n-10-5d_1	1nM
SA323475	1n-24-36_5	1nM
SA323476	1n-24-36_2	1nM
SA323477	1n-24-36_3	1nM
SA323478	1n-24-36_4	1nM
SA323479	250n-24-36_2	250nM
SA323480	250n-24-36_1	250nM
SA323481	250n-16-24_5	250nM
SA323482	250n-16-24_6	250nM
SA323483	250n-16-24_4	250nM
SA323484	250n-10-5d_1	250nM
SA323485	250n-16-24_3	250nM
SA323486	250n-10-5d_2	250nM
SA323487	250n-24-36_6	250nM
SA323488	250n-24-36_5	250nM
SA323489	250n-24-36_4	250nM
SA323490	250n-24-36_3	250nM
SA323491	250n-10-16_2	250nM
SA323492	250n-10-5d_4	250nM
SA323493	250n-10-5d_5	250nM
SA323494	250n-10-5d_6	250nM
SA323495	250n-10-5d_3	250nM
SA323496	250n-10-16_1	250nM
SA323497	250n-10-16_3	250nM
SA323498	250n-16-24_1	250nM
SA323499	250n-10-16_6	250nM
SA323500	250n-10-16_5	250nM
SA323501	250n-16-24_2	250nM
SA323502	250n-10-16_4	250nM
SA323503	0_2	Control
SA323504	0_5	Control
SA323505	0_1	Control
SA323506	0_6	Control
SA323507	0_4	Control
SA323508	0_3	Control

Showing results 1 to 78 of 78

Showing results 1 to 78 of 78

Collection:

Collection ID:	CO003087
Collection Summary:	Wild-type zebrafish embryos (AB line) were maintained in fish water (0.2 % Instant Ocean Salt in deionized water, pH 6.5-8.5, conductivity 450-550 μS.cm-1 and hardness 50-100 mg·L-1 CaCO3) on a 14-h light:10-h dark cycle at 28 °C. Thirty-three embryos were placed in each well of a 6-well plate (Nest Biotech., China) in 3 mL fresh fish water. Zebrafish embryos were exposed to DM from 10-16, 16-24, 24-36 and 10-120 hpf, respectively. DM was washed out after each restricted time point, and zebrafish were assayed at the larval stage, 120 hpf. At each exposure stage, zebrafish embryos were given DM dosages of 1, 100, and 250 nM. The control group received an equivalent volume of dimethyl sulfoxide (DMSO). In each group, 3 wells of zebrafish were utilized to evaluate developmental toxicity, locomotor behavior and apoptotic cells in the central nervous system, while 6 wells of zebrafish were created as separate biological replicates for subsequent lipidomics analysis and kept immediately at -80 °C.
Sample Type:	Zebrafish

Treatment:

Treatment ID:	TR003103
Treatment Summary:	Wild-type zebrafish embryos (AB line) were maintained in fish water (0.2 % Instant Ocean Salt in deionized water, pH 6.5-8.5, conductivity 450-550 μS.cm-1 and hardness 50-100 mg·L-1 CaCO3) on a 14-h light:10-h dark cycle at 28 °C. Thirty-three embryos were placed in each well of a 6-well plate (Nest Biotech., China) in 3 mL fresh fish water. Zebrafish embryos were exposed to DM from 10-16, 16-24, 24-36 and 10-120 hpf, respectively. DM was washed out after each restricted time point, and zebrafish were assayed at the larval stage, 120 hpf. At each exposure stage, zebrafish embryos were given DM dosages of 1, 100, and 250 nM. The control group received an equivalent volume of dimethyl sulfoxide (DMSO). In each group, 3 wells of zebrafish were utilized to evaluate developmental toxicity, locomotor behavior and apoptotic cells in the central nervous system, while 6 wells of zebrafish were created as separate biological replicates for subsequent lipidomics analysis and kept immediately at -80 °C.

Treatment ID:

TR003103

Treatment Summary:

Wild-type zebrafish embryos (AB line) were maintained in fish water (0.2 % Instant Ocean Salt in deionized water, pH 6.5-8.5, conductivity 450-550 μS.cm-1 and hardness 50-100 mg·L-1 CaCO3) on a 14-h light:10-h dark cycle at 28 °C. Thirty-three embryos were placed in each well of a 6-well plate (Nest Biotech., China) in 3 mL fresh fish water. Zebrafish embryos were exposed to DM from 10-16, 16-24, 24-36 and 10-120 hpf, respectively. DM was washed out after each restricted time point, and zebrafish were assayed at the larval stage, 120 hpf. At each exposure stage, zebrafish embryos were given DM dosages of 1, 100, and 250 nM. The control group received an equivalent volume of dimethyl sulfoxide (DMSO). In each group, 3 wells of zebrafish were utilized to evaluate developmental toxicity, locomotor behavior and apoptotic cells in the central nervous system, while 6 wells of zebrafish were created as separate biological replicates for subsequent lipidomics analysis and kept immediately at -80 °C.

Sample Preparation:

Sampleprep ID:	SP003100
Sampleprep Summary:	Each replicate of zebrafish (1 well, ~10 mg) was accurately weighted and transferred to an Eppendorf tube. Then, each sample was spiked with 400 μL of precooled methanol containing lipid standards (i.e., 1.5 μg/mL of phosphatidylcholine (PC) (19:0/19:0), 1.5 μg/mL of phosphatidylethanolamine (PE) (15:0/15:0), 1.5 μg/mL of lysophospatidylcholine (LPC) (19:0), 1.5 μg/mL of sphingomyelin (SM) (d18:1/12:0), 1.2 μg/mL of triacylglycerol (TG) (15:0/15:0/15:0), 1.2 μg/mL of ceramide (Cer) (d18:1/17:0), 1 μg/mL of palmitic acid (fatty acid (FA) (16:0))-d3, and 1 μg/mL of stearic acid (FA(18:0))-d3), followed by tissue homogenization using the bead-based homogenizer (Tissuelyser-24, Shanghai jingxin industrial development co., ltd, China) for 1 min at 65 Hz. Next, 1 mL of tert-butyl methyl ether (MTBE) was added to the tube, and the mixture was thoroughly vortexed (1,000 rpm, 10 ℃, 30 min). Phase separation was induced by adding 400 μL of Milli-Q water and centrifugation at 15,000 g for 15 min at 6 ℃. The upper hydrophobic phase was collected and vacuum-dried in in a refrigerated CentriVap concentrator (Labconco, USA), followed by storage at -80 °C until subsequent analysis.

Sampleprep ID:

SP003100

Sampleprep Summary:

Each replicate of zebrafish (1 well, ~10 mg) was accurately weighted and transferred to an Eppendorf tube. Then, each sample was spiked with 400 μL of precooled methanol containing lipid standards (i.e., 1.5 μg/mL of phosphatidylcholine (PC) (19:0/19:0), 1.5 μg/mL of phosphatidylethanolamine (PE) (15:0/15:0), 1.5 μg/mL of lysophospatidylcholine (LPC) (19:0), 1.5 μg/mL of sphingomyelin (SM) (d18:1/12:0), 1.2 μg/mL of triacylglycerol (TG) (15:0/15:0/15:0), 1.2 μg/mL of ceramide (Cer) (d18:1/17:0), 1 μg/mL of palmitic acid (fatty acid (FA) (16:0))-d3, and 1 μg/mL of stearic acid (FA(18:0))-d3), followed by tissue homogenization using the bead-based homogenizer (Tissuelyser-24, Shanghai jingxin industrial development co., ltd, China) for 1 min at 65 Hz. Next, 1 mL of tert-butyl methyl ether (MTBE) was added to the tube, and the mixture was thoroughly vortexed (1,000 rpm, 10 ℃, 30 min). Phase separation was induced by adding 400 μL of Milli-Q water and centrifugation at 15,000 g for 15 min at 6 ℃. The upper hydrophobic phase was collected and vacuum-dried in in a refrigerated CentriVap concentrator (Labconco, USA), followed by storage at -80 °C until subsequent analysis.

Combined analysis:

Analysis ID	AN004899	AN004900
Analysis type	MS	MS
Chromatography type	Reversed phase	Reversed phase
Chromatography system	Thermo Dionex Ultimate 3000	Thermo Dionex Ultimate 3000
Column	Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)	Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
MS Type	ESI	ESI
MS instrument type	Orbitrap	Orbitrap
MS instrument name	Thermo Orbitrap Exploris 120	Thermo Orbitrap Exploris120
Ion Mode	POSITIVE	NEGATIVE
Units	Peak area	Peak area

Chromatography:

Chromatography ID:	CH003696
Instrument Name:	Thermo Dionex Ultimate 3000
Column Name:	Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
Column Temperature:	55
Flow Gradient:	First maintain 32% B for 1.5 minutes, then linearly increase from 1.5 to 15.5 minutes to 85% B, then linearly increase to 97% B at 0.1 minutes, maintain for 18 minutes, and then rapidly decrease to 32% B at 0.1 minutes, equilibrate until the next injection
Flow Rate:	0.4 mL/min
Solvent A:	Acetonitrile/water (6:4, v/v)
Solvent B:	Isopropanol/acetonitrile (9:1, v/v)
Chromatography Type:	Reversed phase

MS:

MS ID:	MS004643
Analysis ID:	AN004899
Instrument Name:	Thermo Orbitrap Exploris 120
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Xcalibular
Ion Mode:	POSITIVE

MS ID:	MS004644
Analysis ID:	AN004900
Instrument Name:	Thermo Orbitrap Exploris120
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Xcalibular
Ion Mode:	NEGATIVE