Summary of Study ST002997

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001867. The data can be accessed directly via it's Project DOI: 10.21228/M8CX3R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002997
Study TitleMetabolomic analysis reveal mechanisms of Shenzao dripping pill against myocardial ischemia
Study TypeMetabolomic analysis on serum samples of SZDP against MI
Study SummaryThis study employed the advanced Ultra-high performance liquid chromatography-quadruple-Exactive Orbitrap mass spectrometry technology to detect biomarkers related to myocardial ischemia in rats. Additionally, it aimed to investigate the effect of the Shenzao dripping pill on rectifying metabolic disorders and preserving the metabolites in dynamic balance during myocardial ischemia, analyze potential metabolic pathways, and elucidate the mechanism of action of SZDP through the lens of metabolomics.
Institute
Guangdong Pharmaceutical University
DepartmentEngineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province
LaboratoryKey Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine
Last NameKuang
First NameJiehui
AddressWaihuan East Road, 280, Guangzhou, Guangdong, 510006, China
Emailkuang_jieh@163.com
Phone(020)-222-3333
Submit Date2023-12-04
Raw Data AvailableYes
Raw Data File Type(s)mzML, raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-12-21
Release Version1
Jiehui Kuang Jiehui Kuang
https://dx.doi.org/10.21228/M8CX3R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001867
Project DOI:doi: 10.21228/M8CX3R
Project Title:SZDP Metabolomic studies
Project Type:LC_MS
Project Summary:A metabolomics study was conducted to elucidate the metabolic mechanism of Shenzao dripping pill against myocardial ischemia.
Institute:Guangdong Pharmaceutical University
Department:Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province
Laboratory:Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine
Last Name:Kuang
First Name:Jiehui
Address:Waihuan East Road, 280, Guangzhou, Guangdong, 510006, China
Email:kuang_jieh@163.com
Phone:(020)-222-3333
Funding Source:This research was funded by the Project of the Science and Technology Plan Project of Guangzhou (No. 201803010115), the National Natural Science Foundation of China (No. 82173972), and the National Major

Subject:

Subject ID:SU003110
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA326407M7Coronary ligation surgery
SA326408M8Coronary ligation surgery
SA326409M6Coronary ligation surgery
SA326410M1Coronary ligation surgery
SA326411M9Coronary ligation surgery
SA326412M2Coronary ligation surgery
SA326413M5Coronary ligation surgery
SA326414M3Coronary ligation surgery
SA326415M4Coronary ligation surgery
SA326424S4Sham
SA326425S2Sham
SA326426S1Sham
SA326427S3Sham
SA326428S5Sham
SA326429S7Sham
SA326430S6Sham
SA326431S8Sham
SA326416SZ4SZDP-H
SA326417SZ1SZDP-H
SA326418SZ3SZDP-H
SA326419SZ5SZDP-H
SA326420SZ7SZDP-H
SA326421SZ2SZDP-H
SA326422SZ8SZDP-H
SA326423SZ6SZDP-H
Showing results 1 to 25 of 25

Collection:

Collection ID:CO003103
Collection Summary:Blood was collected from abdominal aorta, and the serum samples were obtained by separating the supernatant from the blood centrifugation with 3,500 rpm for 10 min after 30 min of coagulation and stored in the refrigerator at −80°C immediately.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR003119
Treatment Summary:Rats in the model and sham groups were given a solution of 0.5% CMC-Na; in the high dose of the SZDP group was administered 500 mg/kg SZDP. All rats were subjected to intragastric administration once daily.

Sample Preparation:

Sampleprep ID:SP003116
Sampleprep Summary:In each 200 μL aliquot of serum sample, 600 μL of acetonitrile was added and the mixture was thoroughly mixed for a duration of 1 minute. Then, the mixture was subjected to centrifugation at 13000 rpm and 4 °C for a period of 15 min. The supernatant was collected and evaporated to dryness with nitrogen. Methanol (100 μL) was added, and the reconstituted mixture was vortexed and centrifuged at 13000 rpm and a temperature of 4 °C for 15 min.

Combined analysis:

Analysis ID AN004922 AN004923
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH003716
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
Column Temperature:40 °C
Flow Gradient:0–2 minutes, 5% B; 2–5 minutes, 5%–50% B; 5–6 minutes, 50% B; 6–18 minutes, 50%–100% B.
Flow Rate:0.4 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS004665
Analysis ID:AN004922
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Mass spectrometry was set to positive and negative ion switching mode using an electrospray ionization source operated at 350 °C. The specific MS conditions were established: the sheath gas flow rate was 55 arb. Units; the auxiliary gas flow rate was 15 arb. Units with a temperature of 200 °C; the electrospray voltage was 3.5 kV in positive ion mode and -3.8 kV in negative ion mode.
Ion Mode:POSITIVE
  
MS ID:MS004666
Analysis ID:AN004923
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Mass spectrometry was set to positive and negative ion switching mode using an electrospray ionization source operated at 350 °C. The specific MS conditions were established: the sheath gas flow rate was 55 arb. Units; the auxiliary gas flow rate was 15 arb. Units with a temperature of 200 °C; the electrospray voltage was 3.5 kV in positive ion mode and -3.8 kV in negative ion mode.
Ion Mode:NEGATIVE
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