Summary of Study ST003042
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001893. The data can be accessed directly via it's Project DOI: 10.21228/M81F0P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003042 |
| Study Title | The metabolomic resetting effect of DY131 in cisplatin-induced AKI |
| Study Type | MS |
| Study Summary | LC-MS/MS analyses were performed using renal tissues from cisplatin-induced AKI mice with or without DY131 treatment. The data revealed that DY131 alleviated cisplatin-induced mitochondrial dysfunction and energy metabolism disorder, as well as multiple metabolic disorders. |
| Institute | Children's Hospital of Nanjing Medical University |
| Department | Department of Nephrology, State Key Laboratory of Reproductive Medicine |
| Laboratory | Nanjing Key Lab of Pediatrics, Jiangsu Key Laboratory of Pediatrics |
| Last Name | Lu |
| First Name | Lingling |
| Address | Guangzhou Road 72, Nanjing, Jiangsu, 210000, China |
| lulingling89tara@163.com | |
| Phone | 0086-25-8311-7435 |
| Submit Date | 2023-12-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-02-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001893 |
| Project DOI: | doi: 10.21228/M81F0P |
| Project Title: | The metabolomic resetting effect of DY131 in cisplatin-induced AKI |
| Project Type: | MS quantitative analysis |
| Project Summary: | Acute kidney injury (AKI) remains a challenge in clinical practice, and mitochondrial injury is a hallmark of AKI independent of the exact aetiology. ERRγ is a member of orphan nuclear receptors which plays a regulatory role in mitochondrial biosynthesis, energy metabolism, oxidative stress, cell apoptosis, inflammation, and especially metabolic pathways. Here we investigate the role of pharmacological agonist of ERRγ, DY131, in AKI mice induced by cisplatin, IR and LPS. DY131 ameliorated renal function, tubular injury, cell apoptosis and inflammation in AKI mice with multiple causes. Furthermore, we performed LC-MS/MS analyses using renal tissues from cisplatin-induced AKI mice with or without DY131 treatment. Strikingly, the data revealed that DY131 alleviated cisplatin-induced mitochondrial dysfunction and energy metabolism disorder, as well as multiple metabolic disorders. Taken together, the findings highlighted the protective effect of DY131 on AKI probably via improving mitochondrial function and energy metabolism. |
| Institute: | Children's Hospital of Nanjing Medical University |
| Department: | Department of Nephrology, State Key Laboratory of Reproductive Medicine |
| Laboratory: | Nanjing Key Lab of Pediatrics, Jiangsu Key Laboratory of Pediatrics |
| Last Name: | Lu |
| First Name: | Lingling |
| Address: | Guangzhou Road 72, Nanjing, Jiangsu, 210000, China |
| Email: | lulingling89tara@163.com |
| Phone: | 0086-25-8311-7435 |
Subject:
| Subject ID: | SU003157 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 7-8 weeks |
| Weight Or Weight Range: | 20-25g |
| Gender: | Male |
| Animal Animal Supplier: | Nanjing Medical University |
| Animal Housing: | Nanjing Medical University |
| Animal Feed: | Free to food |
| Animal Water: | Free to clean water |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA330375 | C_4 | control |
| SA330376 | C_3 | control |
| SA330377 | C_5 | control |
| SA330378 | C_6 | control |
| SA330379 | C_7 | control |
| SA330380 | C_1 | control |
| SA330381 | C_2 | control |
| SA330368 | DY_P_5 | DY131+cisplatin |
| SA330369 | DY_P_6 | DY131+cisplatin |
| SA330370 | DY_P_4 | DY131+cisplatin |
| SA330371 | DY_P_3 | DY131+cisplatin |
| SA330372 | DY_P_1 | DY131+cisplatin |
| SA330373 | DY_P_7 | DY131+cisplatin |
| SA330374 | DY_P_2 | DY131+cisplatin |
| SA330382 | P_4 | vehicle+cisplatin |
| SA330383 | P_2 | vehicle+cisplatin |
| SA330384 | P_1 | vehicle+cisplatin |
| SA330385 | P_3 | vehicle+cisplatin |
| SA330386 | P_5 | vehicle+cisplatin |
| SA330387 | P_6 | vehicle+cisplatin |
| SA330388 | P_7 | vehicle+cisplatin |
| Showing results 1 to 21 of 21 |
Collection:
| Collection ID: | CO003150 |
| Collection Summary: | The mice were euthanized 72 hours after cisplatin injection, and renal tissues were collected and frozen at -80℃. |
| Sample Type: | Kidney |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003166 |
| Treatment Summary: | Wild type C57BL/6J mice were maintained under standard environmental conditions. A single injection of cisplatin (25 mg/kg, i.p.) was used to induce AKI. DY131 was dissolved in DMSO and further diluted with 5% Tween-80. To assess the effect of DY131, the mice were injected with DY131 (5 mg/kg, i.p.) or vehicle one hour before cisplatin injection, repeated every 24 hours. The mice were euthanized 72 hours after cisplatin injection. |
| Treatment Compound: | cisplatin or saline, DY131 or vehicle |
| Treatment Route: | intraperitoneal injection |
| Treatment Dose: | cisplatin at 25 mg/kg, once. DY131 at 5 mg/kg, once a day for 3 days |
| Treatment Dosevolume: | Animal Vet Treatments: Animal Anesthesia: Animal Acclimation Duration: Animal Fasting: Animal Endpoint Euthanasia: Animal Endpoint Tissue Coll. List: Animal Endpoint Tissue Proc. Method: Animal Endpoint Clinical Signs: |
| Treatment Vehicle: | cisplatin dissolved in saline, DY131 dissolved in DMSO and further diluted with 5% Tween-80 |
Sample Preparation:
| Sampleprep ID: | SP003163 |
| Sampleprep Summary: | 25 mg of sample was weighted to an EP tube, and 500 μL extract solution (acetonitrile: methanol: water = 2: 2: 1, with isotopically-labelled internal standard mixture) was added. After 30 s vortex, the samples were homogenized at 35 Hz for 4 min and sonicated for 5 min in ice-water bath. The homogenization and sonication cycle was repeated for 3 times. Then the samples were incubated for 1 h at -40 ℃ and centrifuged at 12000 rpm for 15 min at 4 ℃. The resulting supernatant was transferred to a fresh glass vial for analysis. |
Combined analysis:
| Analysis ID | AN004990 | AN004991 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Fisher Scientific | Thermo Fisher Scientific |
| Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH003770 |
| Methods Filename: | DY131_Chromatography.txt |
| Instrument Name: | Thermo Fisher Scientific |
| Column Name: | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| Column Temperature: | 30 ℃ |
| Flow Gradient: | 0-0.5min, 95% B; 0.5-7min, 95%-65%B; 7-8min, 65%B-40%B; 8-9min, 40%B; 9-9.1min, 40%-95%B; 9.1-12min, 95%B |
| Flow Rate: | 0.5ml/min |
| Solvent A: | water（pH = 9.75) |
| Solvent B: | acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004730 |
| Analysis ID: | AN004990 |
| Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | sheath gas flow rate as 30 Arb, Aux gas flow rate as 25 Arb, capillary temperature 350 ℃, full MS resolution as 60000, MS/MS resolution as 7500, collision energy as 10/30/60 in NCE mode, spray Voltage as 3.6 kV (positive). Metabolites were quantified by relative quantification and expressed by peak area. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | DY131_MS.txt |
| MS ID: | MS004731 |
| Analysis ID: | AN004991 |
| Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | sheath gas flow rate as 30 Arb, Aux gas flow rate as 25 Arb, capillary temperature 350 ℃, full MS resolution as 60000, MS/MS resolution as 7500, collision energy as 10/30/60 in NCE mode, spray Voltage as -3.2 kV (negative). Metabolites were quantified by relative quantification and expressed by peak area. |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | DY131_MS.txt |
