Summary of Study ST003094

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003094
Study TitleLactiplantibacillus plantarum intervention on serum untargeted metabolome of gestational fecal microbiome transplant germ-free mice
Study SummaryThe study aims to investigate whether administration of probiotic L. plantarum could influence profiling of serum untargeted metabolome of germ-free mice. Results showed that there were 23 metabolites significantly different in intervention group.
Institute
Beijing Sanyuan Foods Co. Ltd
Last NameLijun
First NameChen
Address8th yingchang street, yinghai distric
Emailchenlijun@sanyuan.com.cn
Phone01056306594
Submit Date2024-02-17
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-03-08
Release Version1
Chen Lijun Chen Lijun
https://dx.doi.org/10.21228/M85149
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001923
Project DOI:doi: 10.21228/M85149
Project Title:Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice
Project Summary:The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism.
Institute:Beijing Sanyuan Foods Co. Ltd
Last Name:Lijun
First Name:Chen
Address:8th yingchang street, yinghai distric
Email:chenlijun@sanyuan.com.cn
Phone:01056306594

Subject:

Subject ID:SU003209
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Female

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Factor
SA333301908serum control
SA333302909serum control
SA333303188serum control
SA333304185serum control
SA333305120serum intervention
SA333306107serum intervention
SA333307122serum intervention
SA333308907serum intervention
SA333309180serum intervention
SA33331081serum intervention
Showing results 1 to 10 of 10

Collection:

Collection ID:CO003202
Collection Summary:Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C environment
Sample Type:Blood (serum)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003218
Treatment Summary:mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks).

Sample Preparation:

Sampleprep ID:SP003215
Sampleprep Summary:Samples were vortex for 1 min after adding 0.4 mL methanol; then they were centrifuged for 10 min at 12,000 rpm at 4^oC, and the supernatant was dried and resolved using 0.15 mL 2-chloro-l-phenylalanine (4 ppm) solution, and then filtered by 0.22 μm membrane.
Processing Storage Conditions:4℃
Extract Storage:-80℃

Combined analysis:

Analysis ID AN005064 AN005065
Analysis type MS MS
Chromatography type LC LC
Chromatography system Thermo Vanquish Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Orbitrap Exploris 120 Thermo Orbitrap Exploris 120
Ion Mode POSITIVE NEGATIVE
Units m/z values m/z values

Chromatography:

Chromatography ID:CH003824
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
Column Temperature:40
Flow Gradient:0-1 min, 2% B; 1-9 min, 2%-50% B; 9-12 min, 50%-98% B; 12-13.5 min, 98% B; 13.5-14 min, 98%-2% B; 14-20 min, 2% B
Flow Rate:0.25 mL/min
Solvent A:100% Water; 0.1% Formic acid
Solvent B:100% Acetonitrile; 0.1% Formic acid
Chromatography Type:LC

MS:

MS ID:MS004802
Analysis ID:AN005064
Instrument Name:Thermo Orbitrap Exploris 120
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:MS1 range was 100-1000 m/z, and the number of data-dependent scans per cycle was 4; MS/MS resolving power was 15000 FWHM, normalized collision energy was 30%, and dynamic exclusion time was automatic. The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment.
Ion Mode:POSITIVE
  
MS ID:MS004803
Analysis ID:AN005065
Instrument Name:Thermo Orbitrap Exploris 120
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:MS1 range was 100-1000 m/z, and the number of data-dependent scans per cycle was 4; MS/MS resolving power was 15000 FWHM, normalized collision energy was 30%, and dynamic exclusion time was automatic. The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment.
Ion Mode:NEGATIVE
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