Summary of Study ST003094
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003094 |
| Study Title | Lactiplantibacillus plantarum intervention on serum untargeted metabolome of gestational fecal microbiome transplant germ-free mice |
| Study Summary | The study aims to investigate whether administration of probiotic L. plantarum could influence profiling of serum untargeted metabolome of germ-free mice. Results showed that there were 23 metabolites significantly different in intervention group. |
| Institute | Beijing Sanyuan Foods Co. Ltd |
| Last Name | Lijun |
| First Name | Chen |
| Address | 8th yingchang street, yinghai distric |
| chenlijun@sanyuan.com.cn | |
| Phone | 01056306594 |
| Submit Date | 2024-02-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-03-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001923 |
| Project DOI: | doi: 10.21228/M85149 |
| Project Title: | Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice |
| Project Summary: | The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism. |
| Institute: | Beijing Sanyuan Foods Co. Ltd |
| Last Name: | Lijun |
| First Name: | Chen |
| Address: | 8th yingchang street, yinghai distric |
| Email: | chenlijun@sanyuan.com.cn |
| Phone: | 01056306594 |
Subject:
| Subject ID: | SU003209 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA333301 | 908 | control |
| SA333302 | 909 | control |
| SA333303 | 188 | control |
| SA333304 | 185 | control |
| SA333305 | 120 | intervention |
| SA333306 | 107 | intervention |
| SA333307 | 122 | intervention |
| SA333308 | 907 | intervention |
| SA333309 | 180 | intervention |
| SA333310 | 81 | intervention |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO003202 |
| Collection Summary: | Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C environment |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003218 |
| Treatment Summary: | mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003215 |
| Sampleprep Summary: | Samples were vortex for 1 min after adding 0.4 mL methanol; then they were centrifuged for 10 min at 12,000 rpm at 4^oC, and the supernatant was dried and resolved using 0.15 mL 2-chloro-l-phenylalanine (4 ppm) solution, and then filtered by 0.22 μm membrane. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Chromatography:
| Chromatography ID: | CH003824 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0-1 min, 2% B; 1-9 min, 2%-50% B; 9-12 min, 50%-98% B; 12-13.5 min, 98% B; 13.5-14 min, 98%-2% B; 14-20 min, 2% B |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 100% Water; 0.1% Formic acid |
| Solvent B: | 100% Acetonitrile; 0.1% Formic acid |
| Chromatography Type: | LC |
Analysis:
| Analysis ID: | AN005064 |
| Analysis Type: | MS |
| Chromatography ID: | CH003824 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST003094_AN005064_Results.txt |
| Units: | m/z values |
| Analysis ID: | AN005065 |
| Analysis Type: | MS |
| Chromatography ID: | CH003824 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST003094_AN005065_Results.txt |
| Units: | m/z values |
