Summary of Study ST003097
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003097 |
| Study Title | Lactiplantibacillus plantarum intervention on cecum bile acids of gestational fecal microbiome transplant germ-free mice |
| Study Summary | The study aims to investigate whether administration of probiotic L. plantarum could influence profiling of cecum bile acids of germ-free mice. L. plantarum during gestation of humanized GF mice showed that allolithocholic acid was significantly increased. Other bile acids showed no significant difference. |
| Institute | Beijing Sanyuan Foods Co. Ltd |
| Last Name | Lijun |
| First Name | Chen |
| Address | 8th yingchang street, yinghai district |
| chenlijun@sanyuan.com.cn | |
| Phone | 01056306594 |
| Submit Date | 2024-02-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-03-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001923 |
| Project DOI: | doi: 10.21228/M85149 |
| Project Title: | Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice |
| Project Summary: | The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism. |
| Institute: | Beijing Sanyuan Foods Co. Ltd |
| Last Name: | Lijun |
| First Name: | Chen |
| Address: | 8th yingchang street, yinghai distric |
| Email: | chenlijun@sanyuan.com.cn |
| Phone: | 01056306594 |
Subject:
| Subject ID: | SU003212 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Factor |
|---|---|---|---|
| SA333331 | 908MN | cecum content | control |
| SA333332 | 185MN | cecum content | control |
| SA333333 | 188MN | cecum content | control |
| SA333334 | 909MN | cecum content | control |
| SA333335 | 122MN | cecum content | intervention |
| SA333336 | 120MN | cecum content | intervention |
| SA333337 | 907MN | cecum content | intervention |
| SA333338 | 180MN | cecum content | intervention |
| SA333339 | 81MN | cecum content | intervention |
| SA333340 | 107MN | cecum content | intervention |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO003205 |
| Collection Summary: | Cecum content sample was collected and stored at -80°C environment for bile acids analysis. |
| Sample Type: | Cecum |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003221 |
| Treatment Summary: | Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003218 |
| Sampleprep Summary: | Sample was extracted in 400 μL of methanol, and vortexing and shaking for 60 s, add 100 mg of glass beads, and put into a high-throughput tissue grinder, grinding at 55 Hz for 60 s. Repeat the above operation at least twice; ultrasonic at room temperature for 30 min, centrifuged at 12,000 rpm at 4 ℃ for 10 min, and 200 μL of supernatant mixed with 400 μL of water. Take 200 μL of supernatant and add 400 μL of water, vortex for 30 s; take 300 μL of supernatant and filter it through 0.22 μm membrane, vortex for 30 s. After centrifugation, take 20 μL of supernatant and dilute it 10 times with 180 μL of 30% methanol solution, and add it to the assay vial. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN005068 |
|---|---|
| Analysis type | MS |
| Chromatography type | LC |
| Chromatography system | Shimmadzu LC-30 |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 6500+ QTrap |
| Ion Mode | NEGATIVE |
| Units | μg/g |
Chromatography:
| Chromatography ID: | CH003827 |
| Instrument Name: | Shimmadzu LC-30 |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0-9 min, 30% B; 9-14 min, 30-36% B;14-18 min, 36-38% B;18-24 min, 38-50% B;24-32 min, 50-75% B;32-33 min, 75-90% B;33-35.5 min, 90-30% B |
| Flow Rate: | 5 μL/min |
| Solvent A: | 0.01% formic acid in water |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | LC |
MS:
| MS ID: | MS004806 |
| Analysis ID: | AN005068 |
| Instrument Name: | ABI Sciex 6500+ QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Ion source temperature 500°C, ion source voltage -4500 V, collision gas 6 psi, air curtain gas 30 psi, nebulizer gas and auxiliary gas both 50 psi. Scanning was performed using multiple reaction monitoring (MRM). The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment. |
| Ion Mode: | NEGATIVE |
