Summary of Study ST003098

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003098
Study TitleLactiplantibacillus plantarum intervention on cecum tryptophans of gestational fecal microbiome transplant germ-free mice
Study SummaryThe study aims to investigate whether administration of probiotic L. plantarum could influence profiling of cecum tryptophans of germ-free mice. L. plantarum intervention during gestation of humanized GF mice showed no significant difference on tryptophans’s content.
Institute
Beijing Sanyuan Foods Co. Ltd
Last NameLijun
First NameChen
Address8th yingchang street, yinghai district
Emailchenlijun@sanyuan.com.cn
Phone01056306594
Submit Date2024-02-18
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-03-08
Release Version1
Chen Lijun Chen Lijun
https://dx.doi.org/10.21228/M85149
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001923
Project DOI:doi: 10.21228/M85149
Project Title:Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice
Project Summary:The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism.
Institute:Beijing Sanyuan Foods Co. Ltd
Last Name:Lijun
First Name:Chen
Address:8th yingchang street, yinghai distric
Email:chenlijun@sanyuan.com.cn
Phone:01056306594

Subject:

Subject ID:SU003213
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Female

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Factor
SA333341908MNcecum content control
SA333342185MNcecum content control
SA333343188MNcecum content control
SA333344909MNcecum content control
SA333345122MNcecum content intervention
SA333346120MNcecum content intervention
SA333347907MNcecum content intervention
SA333348180MNcecum content intervention
SA33334981MNcecum content intervention
SA333350107MNcecum content intervention
Showing results 1 to 10 of 10

Collection:

Collection ID:CO003206
Collection Summary:Cecum content was collected and stored at -80°C environment for tryptophan analysis.
Sample Type:Cecum
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003222
Treatment Summary:Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks).

Sample Preparation:

Sampleprep ID:SP003219
Sampleprep Summary:Sample was extracted in 100 μL of 80% methanol aqueous solution, high throughput tissue grinder grinding for 60 s, then add 900 μL of 10% methanol aqueous solution, high throughput tissue grinder grinding for 120 s. Centrifugation was carried out at 12,000 rpm at 4 ℃ for 10 min, and the supernatant was filtered through the membrane to add an equal amount of the internal standard solution, and then transferred into the vial.
Processing Storage Conditions:4℃
Extract Storage:-80℃

Combined analysis:

Analysis ID AN005069
Analysis type MS
Chromatography type LC
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5000
Ion Mode POSITIVE
Units μg/g

Chromatography:

Chromatography ID:CH003828
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
Column Temperature:40
Flow Gradient:0-2 min, 1% B; 2-3 min, 1-30% B; 3-3.5 min, 30% B; 4.5-8 min, 30-50% B; 8-10 min, 50-95% B; 10-11 min, 95% B; 11-17 min, 95-1% B.
Flow Rate:0.3 mL/min
Solvent A:0.1% formic acid in water
Solvent B:0.1% formic acid in methanol
Chromatography Type:LC

MS:

MS ID:MS004807
Analysis ID:AN005069
Instrument Name:ABI Sciex 5000
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Ion Source Temperature 500°C, Ion Source Voltage 5500 V, Collision Gas 6 psi, Curtain Gas 30 psi, Atomizing Gas and Auxiliary Gas both 50 psi. 45 arbitrary units (AU), 13 AU, 1 AU, 350 °C, and 350 °C for sheath gas, aux gas, sweep gas, ion transfer tube, and vaporizer temperature, respectively. The ion source was operated using heated ESI with an ion spray voltage set at 5500 V in positive ion mode. Polarity switching and scheduled selected reaction monitoring (SRM) were employed.The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment.
Ion Mode:POSITIVE
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