Summary of Study ST003101
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001924. The data can be accessed directly via it's Project DOI: 10.21228/M81B11 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003101 |
| Study Title | Parallel pheromonal, metabolite, and lipid analyses reveal patterns associated with early life transitions and ovary activation in honey bee (Apis mellifera) queens |
| Study Summary | We used a novel pheromone detection method to quantify retinue pheromone (QRP) concurrently with shotgun metabolomics and lipidomics analysis to determine what changes in pheromones and small molecules may underpin differences in age, laying status, and acceptance by workers in honey bee queens. |
| Institute | University of British Columbia |
| Department | Life Sciences Institute |
| Last Name | Alcazar Magana |
| First Name | Armando |
| Address | 2350 Health Sciences Mall, Vancouver, BC, V6T1Z3, Canada |
| armando.alcazarmagana@ubc.ca | |
| Phone | 5416097172 |
| Submit Date | 2024-02-20 |
| Num Groups | 4 |
| Total Subjects | 40 |
| Num Females | 40 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-05-24 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001924 |
| Project DOI: | doi: 10.21228/M81B11 |
| Project Title: | Parallel pheromonal, metabolite, and lipid analyses reveal patterns associated with early life transitions and ovary activation in honey bee (Apis mellifera) queens |
| Project Summary: | We used a novel pheromone detection method to quantify retinue pheromone (QRP) concurrently with shotgun metabolomics and lipidomics analysis to determine what changes in pheromones and small molecules may underpin differences in age, laying status, and acceptance by workers in honey bee queens. |
| Institute: | University of British Columbia |
| Department: | Life Sciences Institute |
| Last Name: | Alcazar Magana |
| First Name: | Armando |
| Address: | 2350 Health Sciences Mall, Vancouver, BC, V6T1Z3, Canada |
| Email: | armando.alcazarmagana@ubc.ca |
| Phone: | 5416097172 |
Subject:
| Subject ID: | SU003216 |
| Subject Type: | Insect |
| Subject Species: | Apis mellifera |
| Gender: | Female |
| Species Group: | Insects |
Factors:
Subject type: Insect; Subject species: Apis mellifera (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA333451 | Q25 | Banked |
| SA333452 | Q24 | Banked |
| SA333453 | Q23 | Banked |
| SA333454 | Q26 | Banked |
| SA333455 | Q28 | Banked |
| SA333456 | Q30 | Banked |
| SA333457 | Q29 | Banked |
| SA333458 | Q22 | Banked |
| SA333459 | Q27 | Banked |
| SA333460 | Q21 | Banked |
| SA333461 | Q18 | Early mated |
| SA333462 | Q19 | Early mated |
| SA333463 | Q20 | Early mated |
| SA333464 | Q12 | Early mated |
| SA333465 | Q17 | Early mated |
| SA333466 | Q11 | Early mated |
| SA333467 | Q16 | Early mated |
| SA333468 | Q13 | Early mated |
| SA333469 | Q14 | Early mated |
| SA333470 | Q15 | Early mated |
| SA333471 | Q35 | Late mated |
| SA333472 | Q31 | Late mated |
| SA333473 | Q32 | Late mated |
| SA333474 | Q36 | Late mated |
| SA333475 | Q34 | Late mated |
| SA333476 | Q38 | Late mated |
| SA333477 | Q33 | Late mated |
| SA333478 | Q37 | Late mated |
| SA333479 | Q40 | Late mated |
| SA333480 | Q39 | Late mated |
| SA333481 | V2 | Virgin |
| SA333482 | V7 | Virgin |
| SA333483 | V9 | Virgin |
| SA333484 | V10 | Virgin |
| SA333485 | V1 | Virgin |
| SA333486 | V8 | Virgin |
| SA333487 | V6 | Virgin |
| SA333488 | V4 | Virgin |
| SA333489 | V5 | Virgin |
| SA333490 | V3 | Virgin |
| Showing results 1 to 40 of 40 |
Collection:
| Collection ID: | CO003209 |
| Collection Summary: | Queens were produced by Scandia Honey in May 2023 in Scandia, AB, Canada, according to standard queen rearing methods. |
| Sample Type: | queen bee head |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003225 |
| Treatment Summary: | very young (≤ 1 day old) larvae were grafted from a single mother colony into queen cell cups (JZBZ) and reared into queen cells in strong cell builder colonies. Ten days after grafting (June 1), queen cells were placed in mating nucleus colonies (nucs). |
Sample Preparation:
| Sampleprep ID: | SP003222 |
| Sampleprep Summary: | two-phase extraction was conducted according to methods previously described at J. Lipid Res. 49, 1137–1146 (2008) |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Chromatography:
| Chromatography ID: | CH003831 |
| Chromatography Summary: | Separation of compounds was achieved using a multigradient method on an Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) (GL Sciences) equipped with a Ph-3 guard column (2 µm, 2.1 x 10 mm |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) |
| Column Temperature: | 55 |
| Flow Gradient: | 0 min (5% B), 0–1 min (5% B), 1–8 min (35% B), 8–10.5 min (99% B), 10.5–14 min (99% B), 14–14.5 min (5% B), and 14.5–18 min (5% B) |
| Flow Rate: | 0.3 ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003832 |
| Chromatography Summary: | Lipids were separated on an ACQUITY UPLC CSH C18 analytical column (130Å, 1.7 µm, 2.1 mm X 100 mm, Waters) with a multi-step elution gradient optimized to resolve polar lipids such as hydroxylated fatty acids analyzed. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 65 |
| Flow Gradient: | 0 min (20% B), 0–2 min (20% B), 2–11 min (80% B), 11–11.5 min (99% B), 11.5–13.2 min (99% B), 13.2–14 min (5% B), and 14–18 min (5% B). |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | 100% water; 10 mM ammonium formate; 0.1% formic acid |
| Solvent B: | 10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN005072 |
| Analysis Type: | MS |
| Chromatography ID: | CH003831 |
| Num Factors: | 4 |
| Num Metabolites: | 100 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST003101_AN005072_Results.txt |
| Units: | Rel Abundance |
| Analysis ID: | AN005073 |
| Analysis Type: | MS |
| Chromatography ID: | CH003831 |
| Num Factors: | 4 |
| Num Metabolites: | 138 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST003101_AN005073_Results.txt |
| Units: | Rel Abundance |
| Analysis ID: | AN005074 |
| Analysis Type: | MS |
| Chromatography ID: | CH003832 |
| Num Factors: | 4 |
| Num Metabolites: | 112 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST003101_AN005074_Results.txt |
| Units: | Rel Abundance |
| Analysis ID: | AN005075 |
| Analysis Type: | MS |
| Chromatography ID: | CH003832 |
| Num Factors: | 4 |
| Num Metabolites: | 146 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST003101_AN005075_Results.txt |
| Units: | Rel Abundance |
