Summary of Study ST003101
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001924. The data can be accessed directly via it's Project DOI: 10.21228/M81B11 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003101 |
| Study Title | Parallel pheromonal, metabolite, and lipid analyses reveal patterns associated with early life transitions and ovary activation in honey bee (Apis mellifera) queens |
| Study Summary | We used a novel pheromone detection method to quantify retinue pheromone (QRP) concurrently with shotgun metabolomics and lipidomics analysis to determine what changes in pheromones and small molecules may underpin differences in age, laying status, and acceptance by workers in honey bee queens. |
| Institute | Life Sciences Institute, The University of British Columbia |
| Last Name | Alcazar Magana |
| First Name | Armando |
| Address | 2350 Health Sciences Mall, Vancouver, BC, V6T1Z3, Canada |
| armando.alcazarmagana@ubc.ca | |
| Phone | 5416097172 |
| Submit Date | 2024-02-20 |
| Num Groups | 4 |
| Total Subjects | 40 |
| Num Females | 40 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-05-24 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001924 |
| Project DOI: | doi: 10.21228/M81B11 |
| Project Title: | Parallel pheromonal, metabolite, and lipid analyses reveal patterns associated with early life transitions and ovary activation in honey bee (Apis mellifera) queens |
| Project Summary: | We used a novel pheromone detection method to quantify retinue pheromone (QRP) concurrently with shotgun metabolomics and lipidomics analysis to determine what changes in pheromones and small molecules may underpin differences in age, laying status, and acceptance by workers in honey bee queens. |
| Institute: | Life Sciences Institute, The University of British Columbia |
| Last Name: | Alcazar Magana |
| First Name: | Armando |
| Address: | 2350 Health Sciences Mall, Vancouver, BC, V6T1Z3, Canada |
| Email: | armando.alcazarmagana@ubc.ca |
| Phone: | 5416097172 |
Subject:
| Subject ID: | SU003216 |
| Subject Type: | Insect |
| Subject Species: | Apis mellifera |
| Gender: | Female |
| Species Group: | Insects |
Factors:
Subject type: Insect; Subject species: Apis mellifera (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Treatment |
|---|---|---|---|
| SA333451 | Q25 | Bee head | Banked |
| SA333452 | Q24 | Bee head | Banked |
| SA333453 | Q23 | Bee head | Banked |
| SA333454 | Q26 | Bee head | Banked |
| SA333455 | Q28 | Bee head | Banked |
| SA333456 | Q30 | Bee head | Banked |
| SA333457 | Q29 | Bee head | Banked |
| SA333458 | Q22 | Bee head | Banked |
| SA333459 | Q27 | Bee head | Banked |
| SA333460 | Q21 | Bee head | Banked |
| SA333461 | Q18 | Bee head | Early mated |
| SA333462 | Q19 | Bee head | Early mated |
| SA333463 | Q20 | Bee head | Early mated |
| SA333464 | Q12 | Bee head | Early mated |
| SA333465 | Q17 | Bee head | Early mated |
| SA333466 | Q11 | Bee head | Early mated |
| SA333467 | Q16 | Bee head | Early mated |
| SA333468 | Q13 | Bee head | Early mated |
| SA333469 | Q14 | Bee head | Early mated |
| SA333470 | Q15 | Bee head | Early mated |
| SA333471 | Q35 | Bee head | Late mated |
| SA333472 | Q31 | Bee head | Late mated |
| SA333473 | Q32 | Bee head | Late mated |
| SA333474 | Q36 | Bee head | Late mated |
| SA333475 | Q34 | Bee head | Late mated |
| SA333476 | Q38 | Bee head | Late mated |
| SA333477 | Q33 | Bee head | Late mated |
| SA333478 | Q37 | Bee head | Late mated |
| SA333479 | Q40 | Bee head | Late mated |
| SA333480 | Q39 | Bee head | Late mated |
| SA333481 | V2 | Bee head | Virgin |
| SA333482 | V7 | Bee head | Virgin |
| SA333483 | V9 | Bee head | Virgin |
| SA333484 | V10 | Bee head | Virgin |
| SA333485 | V1 | Bee head | Virgin |
| SA333486 | V8 | Bee head | Virgin |
| SA333487 | V6 | Bee head | Virgin |
| SA333488 | V4 | Bee head | Virgin |
| SA333489 | V5 | Bee head | Virgin |
| SA333490 | V3 | Bee head | Virgin |
| Showing results 1 to 40 of 40 |
Collection:
| Collection ID: | CO003209 |
| Collection Summary: | Queens were produced by Scandia Honey in May 2023 in Scandia, AB, Canada, according to standard queen rearing methods. |
| Sample Type: | queen bee head |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003225 |
| Treatment Summary: | very young (≤ 1 day old) larvae were grafted from a single mother colony into queen cell cups (JZBZ) and reared into queen cells in strong cell builder colonies. Ten days after grafting (June 1), queen cells were placed in mating nucleus colonies (nucs). |
Sample Preparation:
| Sampleprep ID: | SP003222 |
| Sampleprep Summary: | two-phase extraction was conducted according to methods previously described at J. Lipid Res. 49, 1137–1146 (2008) |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN005072 | AN005073 | AN005074 | AN005075 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
| Column | Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) | Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | QTOF | QTOF |
| MS instrument name | Bruker Impact II | Bruker Impact II | Bruker Impact II | Bruker Impact II |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Rel Abundance | Rel Abundance | Rel Abundance | Rel Abundance |
Chromatography:
| Chromatography ID: | CH003831 |
| Chromatography Summary: | Separation of compounds was achieved using a multigradient method on an Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) (GL Sciences) equipped with a Ph-3 guard column (2 µm, 2.1 x 10 mm |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) |
| Column Temperature: | 55 |
| Flow Gradient: | 0 min (5% B), 0–1 min (5% B), 1–8 min (35% B), 8–10.5 min (99% B), 10.5–14 min (99% B), 14–14.5 min (5% B), and 14.5–18 min (5% B) |
| Flow Rate: | 0.3 ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003832 |
| Chromatography Summary: | Lipids were separated on an ACQUITY UPLC CSH C18 analytical column (130Å, 1.7 µm, 2.1 mm X 100 mm, Waters) with a multi-step elution gradient optimized to resolve polar lipids such as hydroxylated fatty acids analyzed. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 65 |
| Flow Gradient: | 0 min (20% B), 0–2 min (20% B), 2–11 min (80% B), 11–11.5 min (99% B), 11.5–13.2 min (99% B), 13.2–14 min (5% B), and 14–18 min (5% B). |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | 100% water; 10 mM ammonium formate; 0.1% formic acid |
| Solvent B: | 10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004810 |
| Analysis ID: | AN005072 |
| Instrument Name: | Bruker Impact II |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | For positive ion mode, the mass spectrometer settings were as follows: capillary voltage of 4500 V, nebulizer gas pressure of 2.0 bar, dry gas flow rate of 9 L min-1, dry gas temperature of 220°C, mass scan range of 60-1300 m/z, and a total cycle time of 0.6 s. To obtain comprehensive structural information, collision energy of 20 V was ramped through each MS/MS scan from 100 to 250%. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004811 |
| Analysis ID: | AN005073 |
| Instrument Name: | Bruker Impact II |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | For negative ionization mode, the capillary voltage was set at -3500 V. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS004812 |
| Analysis ID: | AN005074 |
| Instrument Name: | Bruker Impact II |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Data was collected using data-dependent high-resolution mass spectral acquisition (LC-HRMS/MS - Bruker Impact II) in ESI+ and ESI-. For ESI+, the mass spectrometer settings were as follows: capillary voltage of 4500 V, nebulizer gas pressure of 2.0 bar, dry gas flow rate of 9 L min-1, dry gas temperature of 220°C, mass scan range of 100-1700 m/z. To achieve a lower limits of detection, spectra acquisition rate was set at 3 Hz, and a cycle time of 0.6 s. Collision energy of 20 V was ramped through each MS/MS scan from 100 to 250% |
| Ion Mode: | POSITIVE |
| MS ID: | MS004813 |
| Analysis ID: | AN005075 |
| Instrument Name: | Bruker Impact II |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | For ESI-, the capillary voltage was set at -3800 V. |
| Ion Mode: | NEGATIVE |
