Summary of Study ST003122

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001940. The data can be accessed directly via it's Project DOI: 10.21228/M8ZD94 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003122
Study TitleCharacterizing Metabolic Profiles in Human Hypophysis Tissue According to Hormone Secretion Patterns through NMR Metabolomics
Study SummaryPituitary neoplasms, recently reclassified as Pituitary Neuroendocrine Tumors (PitNETs), present a multidisciplinary challenge, straddling the domains of endocrinology and neurology due to their intricate biological characteristics and symptomatology. These tumors are heterogenous, comprising a diverse array of hormone-producing cell types. Given this complexity, metabolomic methodologies offer significant promise for elucidating the pathophysiological mechanisms underlying these entities. Accordingly, to advance our understanding of the tumor biology, we conducted a comprehensive metabolomic investigation employing NMR spectroscopy. This analysis was performed on pituitary tissues obtained via surgical resection from a cohort comprising 10 patients with hormone-secreting tumors and 12 patients with non-hormone-secreting tumors. Through NMR spectroscopy, our study discovered alterations in 10 distinct metabolites within the tissue samples. These changes encompass O-Phosphoethanolamine, myo-Inositol, choline, alongside various amino acids. Specifically, in the group classified as non-functioning (NF), we noted heightened concentrations of Glutamate, Phenylalanine, Valine, Isoleucine, Tyrosine, and Methionine. Conversely, elevated quantities of O-Phosphoethanolamine (O-PEA), myo-Inositol, Glycine, and Choline were detected in this same cohort
Institute
Bezmialem Vakif University
DepartmentMedical Biochemistry
LaboratorySelek's Lab
Last NameKoktasoglu
First NameFatmanur
AddressPC 34093 Bezmialem Vakif Universitesi Fatih Istanbul
Emailyilmazfatmanur@gmail.com
Phone+905380363138
Submit Date2024-02-20
Num Groups2
Total Subjects22
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2025-08-20
Release Version1
Fatmanur Koktasoglu Fatmanur Koktasoglu
https://dx.doi.org/10.21228/M8ZD94
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001940
Project DOI:doi: 10.21228/M8ZD94
Project Title:Characterizing Metabolic Profiles in Human Hypophysis Tissue According to Hormone Secretion Patterns through NMR Metabolomics
Project Summary:To understand tumor biology in the pituitary gland, metabolomics was employed as a novel approach for determining key metabolic changes.
Institute:Bezmialem Vakif University
Laboratory:Selek's Lab
Last Name:Koktasoglu
First Name:Fatmanur
Address:PC 34093 Bezmialem Vakif Universitesi Fatih Istanbul
Email:yilmazfatmanur@gmail.com
Phone:+905380363138

Subject:

Subject ID:SU003239
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA338115DH_122Functioning
SA338116DH_108Functioning
SA338117DH_152Functioning
SA338118DH_160Functioning
SA338119DH_166Functioning
SA338120DH_76Functioning
SA338121DH_92Functioning
SA338122DH_49Functioning
SA338123DH_37Functioning
SA338124DH_60Functioning
SA338125DH_32Nonfunctioning
SA338126DH_175Nonfunctioning
SA338127DH_296Nonfunctioning
SA338128DH_24Nonfunctioning
SA338129DH_184Nonfunctioning
SA338130DH_48Nonfunctioning
SA338131DH_13Nonfunctioning
SA338132DH_75Nonfunctioning
SA338133DH_115Nonfunctioning
SA338134DH_118Nonfunctioning
SA338135DH_67Nonfunctioning
SA338136DH_43Nonfunctioning
Showing results 1 to 22 of 22

Collection:

Collection ID:CO003232
Collection Summary:The specimens were procured through surgical resection of the pituitary gland. This procedure involved the careful excision of pituitary tissue during surgical interventions aimed at removing pituitary tumors. Post-surgery, the resected tissues were immediately processed and preserved according to standard protocols to maintain their integrity for subsequent metabolomic analysis using NMR spectroscopy
Sample Type:Pituitary gland
Collection Method:Resection from surgical operation
Collection Location:Bezmialem Vakif University, Department of Neurosurgery, Istanbul, Turkey.
Storage Conditions:-80℃
Collection Vials:microcentrifuge tubes
Storage Vials:microcentrifuge tubes

Treatment:

Treatment ID:TR003248
Treatment Summary:No treatment

Sample Preparation:

Sampleprep ID:SP003246
Sampleprep Summary:Samples were stored at -80°C. Following gradual thawing, each sample, weighing 100 mg, was mixed with 1 mL of ice-cold 20 mM phosphate buffer (pH 7.2) per tissue sample. Homogenization was performed using a bead homogenizer. Subsequently, the samples were centrifuged at 10,000 g for 20 minutes at 4°C, and the supernatant was filtered through a cellulose acetate filter. To prepare the NMR samples, 600 µL of the homogenized tissue solution was combined with 100 µL of 1.25 mM Sodium 2-(trimethylsilyl)-1-propanesulfonate-d6 (DSS) prepared in deuterium oxide, and the mixture was thoroughly blended. The resulting solutions were then transferred to NMR tubes.

Analysis:

Analysis ID:AN005116
Laboratory Name:Selek
Analysis Type:NMR
Num Factors:2
Num Metabolites:37
Units:uM

NMR:

NMR ID:NM000277
Analysis ID:AN005116
Instrument Name:Bruker Avance NEO 500 MHz NMR
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:500
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