Summary of Study ST003150

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001958. The data can be accessed directly via it's Project DOI: 10.21228/M8MX5P This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003150
Study TitleImpact of early-life exposure to a potent aryl hydrocarbon receptor ligand on gut microbiota and host glucose homeostasis in C57BL/6J male mice (Part II)
Study SummaryThis study aimed to explore the association between early-life exposure to a potent aryl hydrocarbon receptor (AHR) agonist and persistent disruptions in the microbiota, leading to impaired metabolic homeostasis later in life. This study utilized metagenomics, NMR- and mass spectrometry-based metabolomics, and biochemical assays to analyze the gut microbiome composition and function, as well as the physiological and metabolic effects of early-life exposure to 2,3,7,8-tetrachlorodibenzofuran (TCDF) in conventional, germ-free (GF), and Ahr-null mice. The impact of TCDF on Akkermansia muciniphila (A. muciniphila) in vitro was assessed using optical density (OD 600), flow cytometry, transcriptomics, and mass spectrometry-based metabolomics. TCDF-exposed mice exhibited disruption in the gut microbiome community structure and function, characterized by lower abundances of A. muciniphila, lower levels of cecal short chain fatty acids (SCFAs) and indole-3-lactic acid (ILA), and a reduction in gut hormones GLP-1 and PYY. Importantly, microbial and metabolic phenotypes associated with early-life POP exposure were transferable to GF recipients in the absence of POP carry-over. In addition, AHR-independent interactions between POPs and the microbiota were observed, significantly affected the growth, physiology, gene expression, and metabolic activity of A. muciniphila, resulting in suppressed activity along the ILA pathway.
Institute
Pennsylvania State University
DepartmentDepartment of Veterinary and Biomedical Sciences
Last NameKoo
First NameImhoi
Address307B Life Science Building
Emailiuk41@psu.edu
Phone+1 814-865-7803
Submit Date2024-03-28
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2024-04-30
Release Version1
Imhoi Koo Imhoi Koo
https://dx.doi.org/10.21228/M8MX5P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001958
Project DOI:doi: 10.21228/M8MX5P
Project Title:Impact of early-life exposure to a potent aryl hydrocarbon receptor ligand on gut microbiota and host glucose homeostasis in C57BL/6J male mice
Project Summary:This study aimed to explore the association between early-life exposure to a potent aryl hydrocarbon receptor (AHR) agonist and persistent disruptions in the microbiota, leading to impaired metabolic homeostasis later in life. This study utilized metagenomics, NMR- and mass spectrometry-based metabolomics, and biochemical assays to analyze the gut microbiome composition and function, as well as the physiological and metabolic effects of early-life exposure to 2,3,7,8-tetrachlorodibenzofuran (TCDF) in conventional, germ-free (GF), and Ahr-null mice. The impact of TCDF on Akkermansia muciniphila (A. muciniphila) in vitro was assessed using optical density (OD 600), flow cytometry, transcriptomics, and mass spectrometry-based metabolomics. TCDF-exposed mice exhibited disruption in the gut microbiome community structure and function, characterized by lower abundances of A. muciniphila, lower levels of cecal short chain fatty acids (SCFAs) and indole-3-lactic acid (ILA), and a reduction in gut hormones GLP-1 and PYY. Importantly, microbial and metabolic phenotypes associated with early-life POP exposure were transferable to GF recipients in the absence of POP carry-over. In addition, AHR-independent interactions between POPs and the microbiota were observed, significantly affected the growth, physiology, gene expression, and metabolic activity of A. muciniphila, resulting in suppressed activity along the ILA pathway.
Institute:Pennsylvania State University
Department:Department of Veterinary and Biomedical Sciences
Last Name:Koo
First Name:Imhoi
Address:307B Life Science Building
Email:iuk41@psu.edu
Phone:+1 814-865-7803

Subject:

Subject ID:SU003267
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:4 weeks to 5 months
Gender:Male

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Treatment
SA340917YT201026_14liver TCDF
SA340918YT201026_15liver TCDF
SA340919YT201026_16liver TCDF
SA340920YT200819_110liver TCDF
SA340921YT200819_109liver TCDF
SA340922YT200819_107liver TCDF
SA340923YT200819_108liver TCDF
SA340924YT201026_17liver TCDF
SA340925YT201026_19liver TCDF
SA340926YT210427_19liver TCDF
SA340927YT210427_20liver TCDF
SA340928YT210427_21liver TCDF
SA340929YT210427_18liver TCDF
SA340930YT210427_17liver TCDF
SA340931YT200819_106liver TCDF
SA340932YT210427_16liver TCDF
SA340933YT201026_18liver TCDF
SA340934YT200819_105liver TCDF
SA340935YT200819_11liver TCDF
SA340936YT200819_10liver TCDF
SA340937YT200819_09liver TCDF
SA340938YT200819_08liver TCDF
SA340939YT200819_13liver TCDF
SA340940YT200819_12liver TCDF
SA340941YT200819_06liver vehicle
SA340942YT200819_07liver vehicle
SA340943YT200819_05liver vehicle
SA340944YT200819_04liver vehicle
SA340945YT200819_03liver vehicle
SA340946YT200819_104liver vehicle
SA340947YT200819_103liver vehicle
SA340948YT200819_22liver vehicle
SA340949YT200819_02liver vehicle
SA340950YT200819_101liver vehicle
SA340951YT200819_102liver vehicle
SA340952YT200819_21liver vehicle
Showing results 1 to 36 of 36

Collection:

Collection ID:CO003260
Collection Summary:Liver tissue samples were collected immediately after sacrifice by carbon dioxide asphyxiation and kept at -80°C.
Sample Type:Liver

Treatment:

Treatment ID:TR003276
Treatment Summary:1. After feed training, mice were fed pills containing TCDF (dissolved in acetone) or acetone alone as vehicle, and one pill uniformly contained 0.46 µg TCDF (24 µg/kg as final dose). Mice were housed singly in an empty cage and monitored to ensure the pill was consumed in the morning for 5 days. 2. TCDF (24 µg/kg) or corn oil as vehicle were administered to age-matched male GF and Ahr-/- mice by oral gavage once daily for five days (n = 4 per group). 3. Four-week-old male GF C57BL/6J mice were orally gavaged with 100 µL of cecal suspension (100 mg in 1 mL sterile BHI CHV media) from vehicle or TCDF treated mice in long-duration model. 4. A. muciniphila was administered to GF mice by oral gavage at one dose 107 CFU/0.1 mL suspended in sterile BHI CHV media containing an end concentration of glycerol (15% vol/vol).

Sample Preparation:

Sampleprep ID:SP003274
Sampleprep Summary:For liver lipid quantification, 50 mg of liver tissue was homogenized in 1 mL pre-cooled chloroform/methanol mix [1:1 (v/v)], followed by adding 296 µL water. After centrifugation (10,000 g, 4°C) for 10 min, the lower phase was dried in a vacuum and reconstituted in 600 µL of deuterated chloroform containing 0.03% (v/v) tetramethylsilane (TMS).
Sampleprep Protocol Filename:PSU_Methyl_esterification_GCMS_032724.pdf

Combined analysis:

Analysis ID AN005168
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975C
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH003910
Instrument Name:Agilent 7890A
Column Name:Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)
Column Temperature:325
Flow Gradient:N/A
Flow Rate:0.5658 mL/min
Solvent A:N/A
Solvent B:N/A
Chromatography Type:GC

MS:

MS ID:MS004903
Analysis ID:AN005168
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:For ionization, the EI source was used with the following settings: filament source temperature at 230 °C and electron ionization energy at 70 eV. Mass spectra were collected in a mass range of m/z 50 - 600 at a scan rate of 2 spectra/s. Mass calibration was performed after every injection with internal reference masses m/z 68.9947, 263.9866 and 501.9706. Data acquisition was performed using the Agilent MassHunter Workstation GC-MS Data Acquisition v 10.0 (Agilent Technologies, Waldbronn, Germany).
Ion Mode:POSITIVE
Analysis Protocol File:PSU_tissue_POP_measurement_GCMS_032724.pdf
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