Summary of Study ST003184
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001983. The data can be accessed directly via it's Project DOI: 10.21228/M8D72S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003184 |
| Study Title | A multimodal drug-diet-immunotherapy combination restrains melanoma progression and metastasis - tumor lipidomics |
| Study Summary | Lipidomics profiling of immunocompetent B16F10 model of melanoma to examine lipid levels in tumors following reduced oleic acid content in diet combined with the stearyl CoA desaturase inhibitor CAY10566. |
| Institute | University of Colorado Anschutz Medical Campus |
| Last Name | Haines |
| First Name | Julie |
| Address | 12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA |
| julie.haines@cuanschutz.edu | |
| Phone | 3037243339 |
| Submit Date | 2024-05-01 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-05-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001983 |
| Project DOI: | doi: 10.21228/M8D72S |
| Project Title: | A multimodal drug-diet-immunotherapy combination restrains melanoma progression and metastasis |
| Project Summary: | The genetic landscape of cancer cells can lead to specific metabolic dependencies for tumor growth. Dietary interventions represent an attractive strategy to restrict the availability of key nutrients to tumors. In this study, we identified that growth of a subset of melanoma was severely restricted by a rationally designed combination therapy of a stearoyl-CoA desaturase (SCD) inhibitor with an isocaloric low-oleic acid diet. Despite its importance in oncogenesis, SCD underwent monoallelic co-deletion along with PTEN on chromosome 10q in about 47.5% of melanoma, and the other SCD allele was methylated, resulting in very low SCD expression. While this SCD deficient subset was refractory to SCD inhibitors, the subset of PTEN wildtype melanoma that retained SCD was sensitive. As dietary oleic acid could potentially blunt the effect of SCD inhibitors, a low-oleic acid custom diet was combined with SCD inhibitor. The combination reduced monounsaturated fatty acids and increased saturated fatty acids, inducing robust apoptosis and growth suppression and inhibiting lung metastasis with minimal toxicity in preclinical mouse models of PTEN wildtype melanoma. When combined with anti-PD1 immunotherapy, the SCD inhibitor improved T cell functionality and further constrained melanoma growth in mice. Collectively, these results suggest that optimizing SCD inhibitors with diets low in oleic acid may offer a viable and efficacious therapeutic approach for improving melanoma treatment. |
| Institute: | University of Colorado Anschutz Medical Campus |
| Laboratory: | Lab of Angelo D'Alessandro in collaboration with lab of Biplab Dasgupta |
| Last Name: | Haines |
| First Name: | Julie |
| Address: | 12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA |
| Email: | julie.haines@cuanschutz.edu |
| Phone: | 3037243339 |
Subject:
| Subject ID: | SU003303 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Gender: | Not applicable |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | factor |
|---|---|---|
| SA346767 | DS2-039-016 | SCD inhibitor + high oleic acid diet |
| SA346768 | DS2-039-017 | SCD inhibitor + high oleic acid diet |
| SA346769 | DS2-039-018 | SCD inhibitor + high oleic acid diet |
| SA346770 | DS2-039-019 | SCD inhibitor + high oleic acid diet |
| SA346771 | DS2-039-020 | SCD inhibitor + high oleic acid diet |
| SA346772 | DS2-039-010 | SCD inhibitor + low oleic acid diet |
| SA346773 | DS2-039-009 | SCD inhibitor + low oleic acid diet |
| SA346774 | DS2-039-007 | SCD inhibitor + low oleic acid diet |
| SA346775 | DS2-039-006 | SCD inhibitor + low oleic acid diet |
| SA346776 | DS2-039-008 | SCD inhibitor + low oleic acid diet |
| SA346777 | DS2-039-015 | Vehicle + high oleic acid diet |
| SA346778 | DS2-039-014 | Vehicle + high oleic acid diet |
| SA346779 | DS2-039-011 | Vehicle + high oleic acid diet |
| SA346780 | DS2-039-013 | Vehicle + high oleic acid diet |
| SA346781 | DS2-039-012 | Vehicle + high oleic acid diet |
| SA346782 | DS2-039-003 | Vehicle+ low oleic acid diet |
| SA346783 | DS2-039-002 | Vehicle+ low oleic acid diet |
| SA346784 | DS2-039-005 | Vehicle+ low oleic acid diet |
| SA346785 | DS2-039-001 | Vehicle+ low oleic acid diet |
| SA346786 | DS2-039-004 | Vehicle+ low oleic acid diet |
| Showing results 1 to 20 of 20 |
Collection:
| Collection ID: | CO003296 |
| Collection Summary: | Mice were randomized into groups with combinations of sunflower or coconut oil diet, and vehicle or CAY10566. B16F10 cells were implanted in the flank of C57BL/6 mice. Mice were treated with vehicle or CAY10566 at 30 mg/kg twice daily by oral gavage three days after tumor cell implantation (bilateral tumors in n=4 per group). Mice were euthanized and lungs were harvested 17 days after tumor cell injection/14 days after the start of treatment. |
| Sample Type: | Tumor cells |
Treatment:
| Treatment ID: | TR003312 |
| Treatment Summary: | All animal procedures were carried out in accordance with the IACUC-approved protocol of Cincinnati Children’s Hospital Medical Center. Animals were monitored daily by Veterinary Services. For subcutaneous tumor implantation, 1x10^6 B16F10 cells were mixed with matrigel 1:1 and implanted subcutaneously into NOD-SCID IL2Rgnull or C57BL/6 mice, respectively. CAY10566 was suspended in 0.5% Methyl Cellulose in water with 0.2% Tween 80 at 9 parts per 1 part DMSO stock. CAY10566 was administered at 30mg/kg via oral gavage, twice daily, with a drug holiday during the weekends. |
Sample Preparation:
| Sampleprep ID: | SP003310 |
| Sampleprep Summary: | For tumor analysis, tissue was weighed and placed into 1.5mL Eppendorf Safe-Lock tubes that were pre-filled with 1.0mm glass grinder beads (Biospec Products Inc). Cold methanol was then added to each tube so that all final tissue concentrations were 20 mg/mL. Samples were then homogenized via a “Next Advance” Bullet Blender for 5 minutes and subsequently vortexed for 30 minutes. Following vortex, insoluble material was pelleted by centrifugation (18000g, 10 min) and supernatants were isolated for analysis by UHPLC-MS. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Chromatography:
| Chromatography ID: | CH003955 |
| Chromatography Summary: | Negative C18 global lipidomics |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Kinetex C18 (30 x 2.1mm,1.7um) |
| Column Temperature: | 45 |
| Flow Gradient: | 0-3 min 10-95% B at 0.3 mL/min, 3-4.2 min hold at 95% B at 0.3 mL/min, 4.2-4.3 min drop to 10% B at 0.45 mL/min, 4.3-4.9 min hold at 10%B while lowering flow rate to 0.4 mL/min, 4.9-5 min hold at 10%B while lowering flow rate to 0.3 mL/min. |
| Flow Rate: | 0.3-0.4 ml/min |
| Sample Injection: | 10 uL |
| Solvent A: | 75% water/25% acetonitrile; 5 mM ammonium acetate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 5 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003956 |
| Chromatography Summary: | Positive C18 global lipidomics |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Kinetex C18 (30 x 2.1mm,1.7um) |
| Column Temperature: | 45 |
| Flow Gradient: | 0-3 min 30-100% B at 0.3 mL/min, 3-4.2 min hold at 100% B at 0.3 mL/min, 4.2-4.3 min 100-30% B at 0.4 mL/min, 4.3-4.9 min hold at 30%B and 0.4 mL/min, 4.9-5 min hold at 30%B while lowering flow rate to 0.3 mL/min |
| Flow Rate: | 0.3-0.4 ml/min |
| Sample Injection: | 10 uL |
| Solvent A: | 75% water/25% acetonitrile; 5 mM ammonium acetate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 5 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003957 |
| Chromatography Summary: | Negative C18 fatty acids |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 45 |
| Flow Gradient: | 0-1 min 25% B and 0.3 ml/min; 1-2 min 25-50% B and 0.3 mL/min; 2–8 min 50–90% B and 0.3 mL/min; 8–10 min 90–99% B and 0.3 mL/min; 10–14 min hold at 99% B and 0.3 mL/min; 14–14.1 min 99–25% B and 0.3 mL/min; 14.1–16.9 min hold at 25% B and 0.4 mL/min; 16.9–17 min hold at 25% B and resume flow of 0.3 mL/min |
| Flow Rate: | 0.3-0.4 ml/min |
| Solvent A: | 75% water/25% acetonitrile; 5 mM ammonium acetate |
| Solvent B: | 50% isopropanol/45% acetonitrile/5% water; 5 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN005228 |
| Analysis Type: | MS |
| Chromatography ID: | CH003955 |
| Num Factors: | 4 |
| Num Metabolites: | 279 |
| Rt Units: | Minutes |
| Units: | peak area |
| Analysis ID: | AN005229 |
| Analysis Type: | MS |
| Chromatography ID: | CH003956 |
| Num Factors: | 4 |
| Num Metabolites: | 618 |
| Rt Units: | Minutes |
| Units: | peak area |
| Analysis ID: | AN005230 |
| Analysis Type: | MS |
| Chromatography ID: | CH003957 |
| Num Factors: | 4 |
| Num Metabolites: | 9 |
| Rt Units: | Minutes |
| Units: | peak area |
