Summary of Study ST003291

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002043. The data can be accessed directly via it's Project DOI: 10.21228/M8J52B This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003291
Study TitleAtopic dermatitis and STAT3 double null mutant skin biopsies during healing.
Study SummaryTo do this we developed a matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) workflow for high throughput lipid analysis of cutaneous biopsies as well as a cell culture model of wound healing. A total of 51 samples taken from normal and healing tissue in healthy and AD participants were analyzed using this workflow. The goal of our study was to (a) elucidating a broader understanding of the metabolic pathways initiated during wound healing, with a specific interest in lipid signatures, and (b) identify potential therapeutic targets for patients with barrier deficiencies or impaired healing.
Institute
National Institutes of Health
Last NameRatley
First NameGrace
Address9000 Rockville Pike, Bethesda, MD, 20814, USA
Emailgrace.ratley@nih.gov
Phone8507371782
Submit Date2024-06-18
Num Groups3
Total Subjects58
Raw Data AvailableYes
Raw Data File Type(s)imzML
Analysis Type DetailMALDI-MS
Release Date2024-07-25
Release Version1
Grace Ratley Grace Ratley
https://dx.doi.org/10.21228/M8J52B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002043
Project DOI:doi: 10.21228/M8J52B
Project Title:Imaging mass spectrometry evaluation of wound healing in patients with atopic dermatitis with and without STAT3 dominant negative mutations.
Project Summary:Frequent injury due to environmental challenges requires constant restoration of the skin barrier. Specific defects in ceramide lipid metabolism have been associated with inflammatory skin diseases such as atopic dermatitis (AD). The goal of this study was to characterize the lipid landscape and biosynthetic pathways activated during epidermal repair using MALDI imaging mass spectrometry (IMS). A keratinocyte culture model of wound healing confirmed that glycerophospholipids and glycosphingolipids are impacted during cell proliferation and that sialic acid supplementation can decrease wound closure time. In skin biopsies of healing tissue taken from healthy volunteers, specific phospholipids were upregulated, including phosphatidylcholine and sphingolipids, which play critical roles in membrane repair and cell signaling. Comparing controls to patients with AD, we again identified defects in ceramide-related lipid metabolism with specific defects in lysophosphatidylcholine (Lyso PC) 18:1. Topical application of LysoPC 18:1 provided modest clinical improvement in a mouse model of AD. We then applied this workflow to samples taken from patients with eczematous phenotypes due to STAT3 dominant negative hyper IgE syndrome and identified metabolic disruption of thiamine and phosphatidylinositol metabolism. Overall, the results indicate MALDI imaging can be used to characterize changes in lipid metabolism during wound healing and may indicate an underappreciated role of LysoPC 18:1 during tissue repair.
Institute:National Institutes of Health
Department:National Institute of Allergy and Infectious Disease
Laboratory:Epithelial Therapeutics Unit,
Last Name:Ratley
First Name:Grace
Address:9000 Rockville Pike, Bethesda, MD, 20814, USA
Email:grace.ratley@nih.gov
Phone:8507371782

Subject:

Subject ID:SU003411
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group Skin Biopsy
SA356522ad-9-baselineAtopic dermatitis baseline
SA356523ad-20-baselineAtopic dermatitis baseline
SA356524ad-19-baselineAtopic dermatitis baseline
SA356525ad-18-baselineAtopic dermatitis baseline
SA356526ad-17-baselineAtopic dermatitis baseline
SA356527ad-16-baselineAtopic dermatitis baseline
SA356528ad-15-baselineAtopic dermatitis baseline
SA356529ad-14-baselineAtopic dermatitis baseline
SA356530ad-13-baselineAtopic dermatitis baseline
SA356531ad-12-baselineAtopic dermatitis baseline
SA356532ad-11-baselineAtopic dermatitis baseline
SA356533ad-10-baselineAtopic dermatitis baseline
SA356534ad-1-baselineAtopic dermatitis baseline
SA356535ad-8-baselineAtopic dermatitis baseline
SA356536ad-4-baselineAtopic dermatitis baseline
SA356537ad-7-baselineAtopic dermatitis baseline
SA356538ad-2-baselineAtopic dermatitis baseline
SA356539ad-6-baselineAtopic dermatitis baseline
SA356540ad-3-baselineAtopic dermatitis baseline
SA356541ad-5-baselineAtopic dermatitis baseline
SA356542ad-1-healingAtopic dermatitis healing
SA356543ad-20-healingAtopic dermatitis healing
SA356544ad-19-healingAtopic dermatitis healing
SA356545ad-18-healingAtopic dermatitis healing
SA356546ad-17-healingAtopic dermatitis healing
SA356547ad-3-healingAtopic dermatitis healing
SA356548ad-16-healingAtopic dermatitis healing
SA356549ad-2-healingAtopic dermatitis healing
SA356550ad-15-healingAtopic dermatitis healing
SA356551ad-6-healingAtopic dermatitis healing
SA356552ad-7-healingAtopic dermatitis healing
SA356553ad-4-healingAtopic dermatitis healing
SA356554ad-9-healingAtopic dermatitis healing
SA356555ad-10-healingAtopic dermatitis healing
SA356556ad-8-healingAtopic dermatitis healing
SA356557ad-12-healingAtopic dermatitis healing
SA356558ad-5-healingAtopic dermatitis healing
SA356559ad-14-healingAtopic dermatitis healing
SA356560ad-13-healingAtopic dermatitis healing
SA356561ad-11-healingAtopic dermatitis healing
SA356562hv-12-baselineControl baseline
SA356563hv-20-baselineControl baseline
SA356564hv-14-baselineControl baseline
SA356565hv-15-baselineControl baseline
SA356566hv-16-baselineControl baseline
SA356567hv-17-baselineControl baseline
SA356568hv-18-baselineControl baseline
SA356569hv-19-baselineControl baseline
SA356570hv-23-baselineControl baseline
SA356571hv-21-baselineControl baseline
SA356572hv-22-baselineControl baseline
SA356573hv-24-baselineControl baseline
SA356574hv-25-baselineControl baseline
SA356575hv-27-baselineControl baseline
SA356576hv-28-baselineControl baseline
SA356577hv-29-baselineControl baseline
SA356578hv-30-baselineControl baseline
SA356579hv-26-baselineControl baseline
SA356580hv-13-baselineControl baseline
SA356581hv-11-baselineControl baseline
SA356582hv-2-baselineControl baseline
SA356583hv-4-baselineControl baseline
SA356584hv-6-baselineControl baseline
SA356585hv-3-baselineControl baseline
SA356586hv-7-baselineControl baseline
SA356587hv-8-baselineControl baseline
SA356588hv-5-baselineControl baseline
SA356589hv-9-baselineControl baseline
SA356590hv-10-baselineControl baseline
SA356591hv-1-baselineControl baseline
SA356592hv-28-healingControl healing
SA356593hv-1-healingControl healing
SA356594hv-4-healingControl healing
SA356595hv-2-healingControl healing
SA356596hv-26-healingControl healing
SA356597hv-29-healingControl healing
SA356598hv-25-healingControl healing
SA356599hv-3-healingControl healing
SA356600hv-11-healingControl healing
SA356601hv-30-healingControl healing
SA356602hv-23-healingControl healing
SA356603hv-27-healingControl healing
SA356604hv-24-healingControl healing
SA356605hv-22-healingControl healing
SA356606hv-16-healingControl healing
SA356607hv-21-healingControl healing
SA356608hv-12-healingControl healing
SA356609hv-13-healingControl healing
SA356610hv-14-healingControl healing
SA356611hv-8-healingControl healing
SA356612hv-15-healingControl healing
SA356613hv-10-healingControl healing
SA356614hv-7-healingControl healing
SA356615hv-20-healingControl healing
SA356616hv-17-healingControl healing
SA356617hv-5-healingControl healing
SA356618hv-9-healingControl healing
SA356619hv-19-healingControl healing
SA356620hv-18-healingControl healing
SA356621hv-6-healingControl healing
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Collection:

Collection ID:CO003404
Collection Summary:2mm biopsies were taken from the forearm and 3mm biopsies were taken over the same spots 3 days later. Biopsies were embedded in OCT freezing media and flash frozen using liquid nitrogen. Samples were sent to histoserv for sectioning on intelislides (Bruker Daltonik) and stored in a -80 C freezer for later use.
Sample Type:Skin
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003420
Treatment Summary:No treatments were performed. The study was purely observational.

Sample Preparation:

Sampleprep ID:SP003418
Sampleprep Summary:Patient biopsies were embedded in OCT freezing media and flash frozen using liquid nitrogen. Samples were sent to histoserv for sectioning on intelislides (Bruker Daltonik) and stored in a -80 C freezer for later use.

Chromatography:

Chromatography ID:CH004086
Instrument Name:none
Column Name:none
Column Temperature:none
Flow Gradient:none
Flow Rate:none
Solvent A:none
Solvent B:none
Chromatography Type:None (Direct infusion)

Analysis:

Analysis ID:AN005390
Analysis Type:MS
Chromatography ID:CH004086
Num Factors:6
Num Metabolites:430
Units:Intensity
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