Summary of Study ST003294

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002046. The data can be accessed directly via it's Project DOI: 10.21228/M84V61 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples  |  Perform analysis on untargeted data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST003294
Study TitleExploring the Effects of Toxic Road Runoff Contaminants (6PPD-quinone, 9,10-anthraquinone) on Juvenile Salmonids with a Multi-OMICs Approach (Brain)
Study TypeMetabolomics
Study SummaryPacific salmonid populations have been declining predominantly due to anthropogenic factors such as climate change, habitat loss, and water contaminants derived from urban storm water runoff. Growing urbanism has resulted in areas of impervious surfaces leading to increased storm water runoff entering urban rivers and streams. Storm water runoff has been correlated to escalating rates of pre-spawn mortality (PSM) events in coho salmon in Pacific Northwest streams. PSM events in salmonids are characterized by rapid onset symptoms including surface swimming, loss of equilibrium, and fin splaying that precede mass adult population mortality prior to egg fertilization. In 2020, a chemical derived from tires, N-(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine-quinone (6Q), was isolated from storm water runoff and subsequent toxicity tests resulted in acute coho salmon mortality exhibiting a 24-hour dose at fifty percent population mortality (LC50) of 95 ng/L. 6Q is an oxidation product derived from N-(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD), which is used by tire manufacturers as an antioxidant and antiozonant. The toxic mechanism of 6Q is not yet known, but it is suspected to induce an inhibitory effect on cytochrome P450 (CYP450) genes encoding for CYP450 enzymes, which are responsible for xenobiotic metabolism. We hypothesize 6Q exposure affects salmonids’ ability to synthesize essential structural and cell signaling molecules and inhibits their ability to metabolize other water contaminants such as polycyclic hydrocarbons (PAHs). PAHs are a chemical class of concern due to their potential carcinogenic, teratogenic, and mutagenic effects in aquatic organisms. Thus, this project will focus on the commonly detected water PAH contaminant anthraquinone and its impact on salmonids when co-exposed to 6Q. Further, climate change may be a contributing factor to salmonid susceptibility to water contaminants. Increasing global carbon dioxide emissions have affected aquatic species significantly due to the ability of rivers and streams to transport or store atmospheric carbon dioxide, resulting in rapidly rising water temperatures having potential to exacerbate salmonid sensitivity to water contaminant
Institute
Oregon State University
DepartmentEnvironmental and Molecular Toxicology
LaboratoryWater Quality Toxicology
Last NameGarcia-Jaramillo
First NameManuel
Address1007 SW Campus Way, Corvallis, OR, 97331
Emailmanuel.g.jaramillo@oregonstate.edu
Phone541-737-5714
Submit Date2024-06-05
Num Groups6
Total Subjects270
Num MalesNA
Num FemalesNA
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2025-10-01
Release Version1
Manuel Garcia-Jaramillo Manuel Garcia-Jaramillo
https://dx.doi.org/10.21228/M84V61
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002046
Project DOI:doi: 10.21228/M84V61
Project Title:Exploring the Effects of Toxic Road Runoff Contaminants (6PPD-quinone, 9,10-anthraquinone) on Juvenile Salmonids with a Multi-OMICs Approach
Project Type:Untargeted Metabolomics
Project Summary:Pacific salmonid populations have been declining predominantly due to anthropogenic factors such as climate change, habitat loss, and water contaminants derived from urban storm water runoff. Growing urbanism has resulted in areas of impervious surfaces leading to increased storm water runoff entering urban rivers and streams. Storm water runoff has been correlated to escalating rates of pre-spawn mortality (PSM) events in coho salmon in Pacific Northwest streams. PSM events in salmonids are characterized by rapid onset symptoms including surface swimming, loss of equilibrium, and fin splaying that precede mass adult population mortality prior to egg fertilization. In 2020, a chemical derived from tires, N-(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine-quinone (6Q), was isolated from stormwater runoff and subsequent toxicity tests resulted in acute coho salmon mortality exhibiting a 24-hour dose at fifty percent population mortality (LC50) of 95 ng/L. 6Q is an oxidation product derived from N- (1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD), which is used by tire manufacturers as an antioxidant and antiozonant. The toxic mechanism of 6Q is not yet known, but it is suspected to induce an inhibitory effect on cytochrome P450 (CYP450) genes encoding for CYP450 enzymes, which are responsible for xenobiotic metabolism. We hypothesize 6Q exposure affects salmonids’ ability to synthesize essential structural and cell signaling molecules and inhibits their ability to metabolize other water contaminants such as polycyclic hydrocarbons (PAHs). PAHs are a chemical class of concern due to their potential carcinogenic, teratogenic, and mutagenic effects in aquatic organisms. Thus, this project will focus on the commonly detected water PAH contaminant anthraquinone and its impact on salmonids when co-exposed to 6Q. Further, climate change may be a contributing factor to salmonid susceptibility to water contaminants. Increasing global carbon dioxide emissions have affected aquatic species significantly due to the ability of rivers and streams to transport or store atmospheric carbon dioxide, resulting in rapidly rising water temperatures having potential to exacerbate salmonid sensitivity to water contaminant
Institute:Oregon State University
Department:Environmental and Molecular Toxicology
Laboratory:Water Quality Toxicology
Last Name:Garcia-Jaramillo
First Name:Manuel
Address:1007 SW Campus Way, Corvallis, OR, 97331
Email:manuel.g.jaramillo@oregonstate.edu
Phone:541-737-5714

Subject:

Subject ID:SU003416
Subject Type:Fish
Subject Species:Oncorhynchus tshawytscha; Oncorhynchus mykiss; Oncorhynchus kisutch
Taxonomy ID:74940; 8022; 8019
Weight Or Weight Range:3-5 g
Height Or Height Range:NA
Species Group:Fish

Factors:

Subject type: Fish; Subject species: Oncorhynchus tshawytscha; Oncorhynchus mykiss; Oncorhynchus kisutch (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Treatment Species
SA357448119_6Q_B1_3_CH6PPD-quinone Chinook
SA357449230_6Q_B3_3_CH6PPD-quinone Chinook
SA35745011_6Q_B3_2_CH6PPD-quinone Chinook
SA357451161_6Q_B3_1_CH6PPD-quinone Chinook
SA357452225_6Q_B4_1_CH6PPD-quinone Chinook
SA357453194_6Q_B4_2_CH6PPD-quinone Chinook
SA357454265_6Q_B4_3_CH6PPD-quinone Chinook
SA357455133_6Q_B2_1_CH6PPD-quinone Chinook
SA357456124_6Q_B5_1_CH6PPD-quinone Chinook
SA357457223_6Q_B5_2_CH6PPD-quinone Chinook
SA35745876_6Q_B5_3_CH6PPD-quinone Chinook
SA35745938_6Q_B2_3_CH6PPD-quinone Chinook
SA357460126_6Q_B1_1_CH6PPD-quinone Chinook
SA357461142_6Q_B1_2_CH6PPD-quinone Chinook
SA357462261_6Q_B1_2_CO6PPD-quinone Coho
SA3574638_6Q_B2_1_CO6PPD-quinone Coho
SA357464171_6Q_B4_3_CO6PPD-quinone Coho
SA357465234_6Q_B4_2_CO6PPD-quinone Coho
SA357466162_6Q_B5_3_CO6PPD-quinone Coho
SA357467109_6Q_B1_1_CO6PPD-quinone Coho
SA357468111_6Q_B4_1_CO6PPD-quinone Coho
SA357469250_6Q_B3_1_CO6PPD-quinone Coho
SA357470193_6Q_B5_2_CO6PPD-quinone Coho
SA35747188_6Q_B3_2_CO6PPD-quinone Coho
SA357472175_6Q_B3_3_CO6PPD-quinone Coho
SA357473129_6Q_B5_1_CO6PPD-quinone Coho
SA357474158_6Q_B2_3_CO6PPD-quinone Coho
SA357475169_6Q_B1_3_CO6PPD-quinone Coho
SA357476240_6Q_B2_2_CO6PPD-quinone Coho
SA357477257_6Q_B5_2_RT6PPD-quinone Rainbow Trout
SA357478270_6Q_B5_1_RT6PPD-quinone Rainbow Trout
SA357479121_6Q_B5_3_RT6PPD-quinone Rainbow Trout
SA357480127_6Q_B2_2_RT6PPD-quinone Rainbow Trout
SA35748119_6Q_B2_3_RT6PPD-quinone Rainbow Trout
SA357482236_6Q_B4_3_RT6PPD-quinone Rainbow Trout
SA357483253_6Q_B2_1_RT6PPD-quinone Rainbow Trout
SA357484151_6Q_B3_3_RT6PPD-quinone Rainbow Trout
SA357485188_6Q_B1_2_RT6PPD-quinone Rainbow Trout
SA357486167_6Q_B1_1_RT6PPD-quinone Rainbow Trout
SA357487146_6Q_B1_3_RT6PPD-quinone Rainbow Trout
SA357488242_6Q_B4_2_RT6PPD-quinone Rainbow Trout
SA357489217_6Q_B3_2_RT6PPD-quinone Rainbow Trout
SA357490138_6Q_B4_1_RT6PPD-quinone Rainbow Trout
SA357491197_6Q_B3_1_RT6PPD-quinone Rainbow Trout
SA35749257_AQ_B3_3_CHAnthraquinone Chinook
SA357493174_AQ_B4_3_CHAnthraquinone Chinook
SA35749430_AQ_B3_2_CHAnthraquinone Chinook
SA357495148_AQ_B2_3_CHAnthraquinone Chinook
SA35749670_AQ_B4_1_CHAnthraquinone Chinook
SA357497117_AQ_B2_1_CHAnthraquinone Chinook
SA35749826_AQ_B4_2_CHAnthraquinone Chinook
SA357499219_AQ_B3_1_CHAnthraquinone Chinook
SA357500235_AQ_B2_2_CHAnthraquinone Chinook
SA357501149_AQ_B5_1_CHAnthraquinone Chinook
SA357502185_AQ_B1_3_CHAnthraquinone Chinook
SA357503101_AQ_B1_2_CHAnthraquinone Chinook
SA35750472_AQ_B5_3_CHAnthraquinone Chinook
SA35750551_AQ_B1_1_CHAnthraquinone Chinook
SA3575061_AQ_B5_2_CHAnthraquinone Chinook
SA35750765_AQ_B2_1_COAnthraquinone Coho
SA357508218_AQ_B3_2_COAnthraquinone Coho
SA357509131_AQ_B2_3_COAnthraquinone Coho
SA35751056_AQ_B2_2_COAnthraquinone Coho
SA357511110_AQ_B4_3_COAnthraquinone Coho
SA357512226_AQ_B5_2_COAnthraquinone Coho
SA357513164_AQ_B4_1_COAnthraquinone Coho
SA357514195_AQ_B5_1_COAnthraquinone Coho
SA357515221_AQ_B5_3_COAnthraquinone Coho
SA35751623_AQ_B4_2_COAnthraquinone Coho
SA35751742_AQ_B3_1_COAnthraquinone Coho
SA357518155_AQ_B1_1_COAnthraquinone Coho
SA35751928_AQ_B3_3_COAnthraquinone Coho
SA357520227_AQ_B5_3_COAnthraquinone Coho
SA357521134_AQ_B1_3_COAnthraquinone Coho
SA35752297_AQ_B1_2_COAnthraquinone Coho
SA357523256_AQ_B4_3_RTAnthraquinone Rainbow Trout
SA35752486_AQ_B5_2_RTAnthraquinone Rainbow Trout
SA35752522_AQ_B4_1_RTAnthraquinone Rainbow Trout
SA357526192_AQ_B2_3_RTAnthraquinone Rainbow Trout
SA357527249_AQ_B1_2_RTAnthraquinone Rainbow Trout
SA357528210_AQ_B5_3_RTAnthraquinone Rainbow Trout
SA35752950_AQ_B1_3_RTAnthraquinone Rainbow Trout
SA35753079_AQ_B2_2_RTAnthraquinone Rainbow Trout
SA357531243_AQ_B5_1_RTAnthraquinone Rainbow Trout
SA357532143_AQ_B2_1_RTAnthraquinone Rainbow Trout
SA35753381_AQ_B1_1_RTAnthraquinone Rainbow Trout
SA357534245_AQ_B3_2_RTAnthraquinone Rainbow Trout
SA357535215_AQ_B4_2_RTAnthraquinone Rainbow Trout
SA357536147_AQ_B3_3_RTAnthraquinone Rainbow Trout
SA357537216_AQ_B3_1_RTAnthraquinone Rainbow Trout
SA35753837_CE_B2_3_CHCoexposure Chinook
SA35753921_CE_B2_2_CHCoexposure Chinook
SA35754049_CE_B5_3_CHCoexposure Chinook
SA357541199_CE_B3_1_CHCoexposure Chinook
SA357542103_CE_B3_2_CHCoexposure Chinook
SA357543262_CE_B3_3_CHCoexposure Chinook
SA35754489_CE_B2_1_CHCoexposure Chinook
SA35754518_CE_B5_1_CHCoexposure Chinook
SA35754653_CE_B4_1_CHCoexposure Chinook
SA357547239_CE_B1_3_CHCoexposure Chinook
Showing page 1 of 3     Results:    1  2  3  Next     Showing results 1 to 100 of 269

Collection:

Collection ID:CO003409
Collection Summary:Fish were sacrificed with MS222 and livers and brains were dissected, weighed, and flash frozen in aluminum foil packets using liquid nitrogen and stored at -80℃
Sample Type:Brain, Liver
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003425
Treatment Summary:Experimental exposures were conducted using a 2/3 static renewal system every 24-hours for a duration of 120-hours. Phase II experimental design included a 120-hour fasted control tank, a 120-hour fed control tank, and four chemical treatment groups: a 120-hour 1 µg/L 6PPD-q exposure tank, a 120-hour 10 µg/L AQ exposure tank, a 120-hour both 1 µg/L 6PPD-q and 10 µg/L AQ co-exposure tank, and a 120-hour 10 µg/L AQ exposure tank combined with the 24-hour exposure of 1 µg/L 6PPD-q. Each tank included five fish replicates and each condition was repeated to collect three biological replicates (n = 3)
Treatment Compound:6PPD-quinone, Anthraquinone

Sample Preparation:

Sampleprep ID:SP003423
Sampleprep Summary:Liver and brain tissues were allocated into 10 mg samples using a razor and dry ice. Samples were aliquoted in Eppendorf vials with 40 µL of 1.4 mm ceramic beads. Chilled methanol:water, 80:20, was added to the vials (300 µL). Samples were spiked with 3 µL of isotope labeled metabolite Mix 2 QReSS Kit (Cambridge Isotope Labs) to account for extraction variability among tissue samples. Samples were extracted in a Precellys homogenizer (3x15s; 5,500 rpm). Homogenized tissue (200 µL) was transferred to a clean Eppendorf vial with a corresponding randomly assigned ID number. Samples were centrifuged at 10,000g for 3 min at 4 °C. Samples were stored overnight at – 24 °C to precipitated proteins. Samples were centrifuged again at 13,000g for 15 min at 4 °C. Supernatant (150 µL) was recovered in a new Eppendorf tube and stored at 4 °C before LCMS analysis. Samples were spiked with 3 µL of isotope labeled metabolite Mix 1 QReSS Kit (Cambridge Isotope Labs) to account for instrumental variation between runs.

Chromatography:

Chromatography ID:CH004092
Instrument Name:Sciex ExionLC AD
Column Name:GL Sciences Intersil Ph-3 (150 x 2.1 mm, 2µm)
Column Temperature:40℃
Flow Gradient:In %B, at minute 0, 5% B, and held at 5% B until minute 3. From 3-12 minutes a gradient up to 98% B and held at 98% until 17 minutes. From 17 to 17.5 drop to 5% B and held at 5% until 23 minutes.
Flow Rate:0.3 mL/min
Injection Temperature:15
Internal Standard:QReSS Kit
Sample Injection:2 uL
Solvent A:100% Water; 0.1% Formic acid
Solvent B:100% Acetonitrile; 0.1% Formic acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN005397
Analysis Type:MS
Chromatography ID:CH004092
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST003294_AN005397_Results.txt
Units:abundance
  
Analysis ID:AN005398
Analysis Type:MS
Chromatography ID:CH004092
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST003294_AN005398_Results.txt
Units:abundance
  logo