Summary of Study ST003299

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST003299
Study TitleLactiplantibacillus plantarum intervention on fecal tryptophans of gestational fecal microbiome transplant germ-free mice
Study SummaryThe study aims to investigate whether administration of probiotic L. plantarum could influence profiling of fecal tryptophans of germ-free mice. L. plantarum intervention during gestation of humanized GF mice showed that serotonin was significantly increased, other tryptophans no significant difference.
Institute
Beijing Sanyuan Foods Co. Ltd
Last NameLijun
First NameChen
Address8th yingchang street, yinghai district
Emailchenlijun@sanyuan.com.cn
Phone01056306594
Submit Date2024-02-18
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-12-30
Release Version1
Chen Lijun Chen Lijun
https://dx.doi.org/10.21228/M85149
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001923
Project DOI:doi: 10.21228/M85149
Project Title:Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice
Project Summary:The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism.
Institute:Beijing Sanyuan Foods Co. Ltd
Last Name:Lijun
First Name:Chen
Address:8th yingchang street, yinghai distric
Email:chenlijun@sanyuan.com.cn
Phone:01056306594

Subject:

Subject ID:SU003420
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Female
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Factor
SA358070908S0feces control
SA358071185S18feces control
SA358072185S7feces control
SA358073188S0feces control
SA358074188S1feces control
SA358075188S14feces control
SA358076188S18feces control
SA358077188S7feces control
SA358078908S1feces control
SA358079185S1feces control
SA358080908S14feces control
SA358081908S18feces control
SA358082908S7feces control
SA358083909S0feces control
SA358084909S1feces control
SA358085909S14feces control
SA358086909S18feces control
SA358087909S7feces control
SA358088185S14feces control
SA358089185S0feces control
SA358090180S1feces intervention
SA358091907S14feces intervention
SA358092081S14feces intervention
SA358093081S18feces intervention
SA358094081S7feces intervention
SA358095107S0feces intervention
SA358096107S1feces intervention
SA358097107S14feces intervention
SA358098107S18feces intervention
SA358099107S7feces intervention
SA358100120S0feces intervention
SA358101120S1feces intervention
SA358102907S7feces intervention
SA358103907S18feces intervention
SA358104907S1feces intervention
SA358105180S14feces intervention
SA358106907S0feces intervention
SA358107120S14feces intervention
SA358108120S18feces intervention
SA358109120S7feces intervention
SA358110122S0feces intervention
SA358111122S1feces intervention
SA358112122S14feces intervention
SA358113122S18feces intervention
SA358114122S7feces intervention
SA358115180S0feces intervention
SA358116081S1feces intervention
SA358117180S7feces intervention
SA358118180S18feces intervention
SA358119081S0feces intervention
Showing results 1 to 50 of 50

Collection:

Collection ID:CO003413
Collection Summary:Feces were collected at and stored at -80°C for tryptophan analysis.
Sample Type:Feces
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003429
Treatment Summary:Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks).

Sample Preparation:

Sampleprep ID:SP003427
Sampleprep Summary:Sample was extracted in 100 μL of 80% methanol aqueous solution, high throughput tissue grinder grinding for 60 s, then add 900 μL of 10% methanol aqueous solution, high throughput tissue grinder grinding for 120 s. Centrifugation was carried out at 12,000 rpm at 4℃ for 10 min, and the supernatant was filtered through the membrane to add an equal amount of the internal standard solution, and then transferred into the vial
Processing Storage Conditions:4℃
Extract Storage:-80℃

Combined analysis:

Analysis ID AN005406
Analysis type MS
Chromatography type LC
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex API 5000 QQQ
Ion Mode POSITIVE
Units μg/g

Chromatography:

Chromatography ID:CH004099
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
Column Temperature:40℃
Flow Gradient:0-2 min, 1% B; 2-3 min, 1-30% B; 3-3.5 min, 30% B; 4.5-8 min, 30-50% B; 8-10 min, 50-95% B; 10-11 min, 95% B; 11-17 min, 95-1% B.
Flow Rate:0.3 mL/min
Solvent A:100% Water; 0.1% Formic acid
Solvent B:100% Methanol; 0.1% Formic acid
Chromatography Type:LC

MS:

MS ID:MS005133
Analysis ID:AN005406
Instrument Name:ABI Sciex API 5000 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:45 arbitrary units (AU), 13 AU, 1 AU, 350 °C, and 350 °C for sheath gas, aux gas, sweep gas, ion transfer tube, and vaporizer temperature, respectively. The ion source was operated using heated ESI with an ion spray voltage set at 5500 V in positive ion mode. Polarity switching and scheduled selected reaction monitoring (SRM) were employed. The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment.
Ion Mode:POSITIVE
  logo