Summary of Study ST003309
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002057. The data can be accessed directly via it's Project DOI: 10.21228/M8QN7M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003309 |
| Study Title | Needle leachates of Pinus koraiensis from May to September |
| Study Summary | To study the allelopathy in agroforestry system (Pinus koraiensis and Panax ginseng in this context), we identified the compounds of the needle leachates during the growing season of Panax ginseng (May~September). Three replicates were set for each month, leachates were extracted with ethyl acetate and concentrated for GC-MS analysis. As a result, a total of 795 metabolites were identified in all the samples. |
| Institute | Yunnan University |
| Department | School of Agriculture |
| Last Name | Xing-Yu |
| First Name | Ji |
| Address | Road Fengyuan, District Panlong, Kunming, Yunnan, China |
| xingyu.ji@hotmail.com | |
| Phone | ＋86-13354989110 |
| Submit Date | 2024-06-04 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | qgd |
| Analysis Type Detail | GC-MS |
| Release Date | 2024-07-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002057 |
| Project DOI: | doi: 10.21228/M8QN7M |
| Project Title: | Use of untargeted metabolomics for assessing soil from Panax ginseng that were leaf-sprayed with endo-borneol |
| Project Summary: | To study the rhizosphere soil matabolism of 4-years Panax ginseng that wereleaf-sprayed with endo-borneol at different concentrations (0, 0.1 mg/L, and 100 mg/L) under field condition, liquid chromarography tandem mass spectrometry was used for examination. Each treatment is set with 6 replicates for assessing nontargeted metabolomics. The LC-MS/MS analysis was performed using a Thermo UHPLC-Q Exactive HF-X system equipped with an ACQUITY HSS T3 column. As a result, a total of 296 and 569 metabolites were identified in negative ion mode and positive mode, respectively. |
| Institute: | Yunnan University |
| Department: | School of Agriculture |
| Laboratory: | State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan |
| Last Name: | Ji |
| First Name: | Xing-Yu |
| Address: | Yuhua Road, Chenggong, Kunming, Yunnan Province, 650100, China |
| Email: | xingyu.ji@hotmail.com |
| Phone: | ＋86-13354989110 |
| Funding Source: | Natural Science Foundation of China |
| Project Comments: | U23A20202 |
| Publications: | still under review |
| Contributors: | Xing-Yu Ji, Shusheng Zhu |
Subject:
| Subject ID: | SU003430 |
| Subject Type: | Plant |
| Subject Species: | Pinus koraiensis |
| Taxonomy ID: | 88728 |
Factors:
Subject type: Plant; Subject species: Pinus koraiensis (Factor headings shown in green)
| mb_sample_id | local_sample_id | Month | Sample source |
|---|---|---|---|
| SA358834 | Aug_2 | August | Pinus koraiensis leaf |
| SA358835 | Aug_3 | August | Pinus koraiensis leaf |
| SA358836 | Aug_1 | August | Pinus koraiensis leaf |
| SA358837 | July_1 | July | Pinus koraiensis leaf |
| SA358838 | July_2 | July | Pinus koraiensis leaf |
| SA358839 | July_3 | July | Pinus koraiensis leaf |
| SA358840 | June_2 | June | Pinus koraiensis leaf |
| SA358841 | June_3 | June | Pinus koraiensis leaf |
| SA358842 | June_1 | June | Pinus koraiensis leaf |
| SA358843 | May_2 | May | Pinus koraiensis leaf |
| SA358844 | May_3 | May | Pinus koraiensis leaf |
| SA358845 | May_1 | May | Pinus koraiensis leaf |
| SA358846 | Sep_1 | September | Pinus koraiensis leaf |
| SA358847 | Sep_2 | September | Pinus koraiensis leaf |
| SA358848 | Sep_3 | September | Pinus koraiensis leaf |
| SA358849 | solvent_blank1 | solvent blank | extracting solution |
| SA358850 | solvent_blank2 | solvent blank | extracting solution |
| Showing results 1 to 17 of 17 |
Collection:
| Collection ID: | CO003423 |
| Collection Summary: | Fresh pine needles were collected from forests of Pinus koraiensis located in Benxi (41°4′17″ N, 124°29′58″ E, 578 m, 965.9 hPa) , Liaoning province, China. From May to September, 2020, fresh needles were collected on a monthly basis, and brought back to laboratory to prepare needle leachates. Every 150 g of fresh needles collected from Pinus koraiensis on a monthly basis during May to September were soaked in 1000 ml of sterilized water for 48 hours at room temperature, and subsequently filtered twice using qualitative filter paper through suction filtration. The aqueous leachates with a concentration of 150 g/L were stored at 4 °C for further analysis. |
| Sample Type: | Leaf |
Treatment:
| Treatment ID: | TR003439 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP003437 |
| Sampleprep Summary: | A volume of 100 ml of leachates was extracted by 300 ml ethyl acetate (Analytical grade, Tianjin Fengchuan Chemical Reagent Co., Ltd, 426 Tianjin, China) then evaporated by rotary evaporator at 45°C with 4°C-condensed water recycled in low-temperature circulator. The remaining sediment was 429 rinsed with 2.5 ml of hexane (HPLC, EMD Millipore, Billerica, MA, USA). The liquid hexane was filtered using a 0.22 um Millipore filter (Catridge filter, Jin Teng, China) for gas chromatography-mass spectrometry (GC-MS) analysis. |
Combined analysis:
| Analysis ID | AN005421 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Shimadzu GCMS-QP2010 ultra |
| Column | Shimadzu SH-Rxi-5Sil MS (30m x 0.25mm x 0.25um) |
| MS Type | EI |
| MS instrument type | GC-MS |
| MS instrument name | Shimadzu QP2010 Ultra |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH004110 |
| Chromatography Summary: | The temperature program of GC-MS started from an initial temperature of 40 ℃, followed by an increase of 3 ℃/min to 80 ℃ and then an increase of 5 ℃/min from 80 ℃ to 260 ℃, which was maintained for 30 min. |
| Instrument Name: | Shimadzu GCMS-QP2010 ultra |
| Column Name: | Shimadzu SH-Rxi-5Sil MS (30m x 0.25mm x 0.25um) |
| Column Temperature: | 40 - 260 |
| Flow Gradient: | Not applicable |
| Flow Rate: | Not applicable |
| Injection Temperature: | 250 ℃ |
| Solvent A: | Not applicable |
| Solvent B: | Not applicable |
| Analytical Time: | 79.33 min |
| Chromatography Type: | GC |
MS:
| MS ID: | MS005147 |
| Analysis ID: | AN005421 |
| Instrument Name: | Shimadzu QP2010 Ultra |
| Instrument Type: | GC-MS |
| MS Type: | EI |
| MS Comments: | The mass spectrum was recorded for the mass range 35–500 m/z and scanned at a rate of 0.3s. All the compounds identified by GCMS solutions software (Shimadzu, Kyoto, Japan) based on the comparison of its mass spectra (MS) with the NIST14 (National Institute of Standards and Technology) library. |
| Ion Mode: | POSITIVE |
