Summary of Study ST003310
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002057. The data can be accessed directly via it's Project DOI: 10.21228/M8QN7M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003310 |
| Study Title | Determination the concentration of endo-borneol from needle leachates of Pinus koraiensis flushed by precipitation via indoor simulated leaching) |
| Study Summary | The precipitation from April to October (2016-2021) was provided by local weather burea in Benxi. The monthly precipitation and duration in Benxi is classified into high, medium, and low level. Under each precipitation, the concentration of endo-borneol is quantified by indoor simulated leaching. The simulated leachates were extracted by ethyl acetate and then analyzed by GC/MSD. As a result, the peak area of endo-borneol ranges from 2006.257 to 27106.75 under tested precipitation. |
| Institute | Yunnan University |
| Department | School of Agriculture |
| Laboratory | Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China |
| Last Name | Xing-Yu |
| First Name | Ji |
| Address | Road Fengyuan, District Panlong, Kunming, Yunnan, China |
| xingyu.ji@hotmail.com | |
| Phone | ＋86-13354989110 |
| Submit Date | 2024-06-05 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS |
| Release Date | 2024-07-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002057 |
| Project DOI: | doi: 10.21228/M8QN7M |
| Project Title: | Use of untargeted metabolomics for assessing soil from Panax ginseng that were leaf-sprayed with endo-borneol |
| Project Summary: | To study the rhizosphere soil matabolism of 4-years Panax ginseng that wereleaf-sprayed with endo-borneol at different concentrations (0, 0.1 mg/L, and 100 mg/L) under field condition, liquid chromarography tandem mass spectrometry was used for examination. Each treatment is set with 6 replicates for assessing nontargeted metabolomics. The LC-MS/MS analysis was performed using a Thermo UHPLC-Q Exactive HF-X system equipped with an ACQUITY HSS T3 column. As a result, a total of 296 and 569 metabolites were identified in negative ion mode and positive mode, respectively. |
| Institute: | Yunnan University |
| Department: | School of Agriculture |
| Laboratory: | State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan |
| Last Name: | Ji |
| First Name: | Xing-Yu |
| Address: | Yuhua Road, Chenggong, Kunming, Yunnan Province, 650100, China |
| Email: | xingyu.ji@hotmail.com |
| Phone: | ＋86-13354989110 |
| Funding Source: | Natural Science Foundation of China |
| Project Comments: | U23A20202 |
| Publications: | still under review |
| Contributors: | Xing-Yu Ji, Shusheng Zhu |
Subject:
| Subject ID: | SU003431 |
| Subject Type: | Plant |
| Subject Species: | Pinus koraiensis |
| Taxonomy ID: | 88728 |
Factors:
Subject type: Plant; Subject species: Pinus koraiensis (Factor headings shown in green)
| mb_sample_id | local_sample_id | Precipitation | Sample source |
|---|---|---|---|
| SA358851 | standard_1 | 0.05 ng/μL | standard |
| SA358852 | standard_2 | 0.1 ng/μL | standard |
| SA358853 | standard_3 | 0.2 ng/μL | standard |
| SA358854 | standard_4 | 1 ng/μL | standard |
| SA358855 | standard_5 | 2 ng/μL | standard |
| SA358856 | H1 | high level | Pinus koraiensis leaf leachate |
| SA358857 | H2 | high level | Pinus koraiensis leaf leachate |
| SA358858 | H3 | high level | Pinus koraiensis leaf leachate |
| SA358859 | H4 | high level | Pinus koraiensis leaf leachate |
| SA358860 | H5 | high level | Pinus koraiensis leaf leachate |
| SA358861 | H6 | high level | Pinus koraiensis leaf leachate |
| SA358862 | L5 | low level | Pinus koraiensis leaf leachate |
| SA358863 | L6 | low level | Pinus koraiensis leaf leachate |
| SA358864 | L3 | low level | Pinus koraiensis leaf leachate |
| SA358865 | L4 | low level | Pinus koraiensis leaf leachate |
| SA358866 | L1 | low level | Pinus koraiensis leaf leachate |
| SA358867 | L2 | low level | Pinus koraiensis leaf leachate |
| SA358868 | M1 | medium level | Pinus koraiensis leaf leachate |
| SA358869 | M2 | medium level | Pinus koraiensis leaf leachate |
| SA358870 | M3 | medium level | Pinus koraiensis leaf leachate |
| SA358871 | M4 | medium level | Pinus koraiensis leaf leachate |
| SA358872 | M5 | medium level | Pinus koraiensis leaf leachate |
| SA358873 | M6 | medium level | Pinus koraiensis leaf leachate |
| SA358874 | solvent_blank | solvent | solvent |
| Showing results 1 to 24 of 24 |
Collection:
| Collection ID: | CO003424 |
| Collection Summary: | Based on historical meteorological data, monthly precipitations were graded as low (34.6 mm, 771.63 min), medium (104.2 mm, 2223.07 min) and high (285.8 mm, 5233.03 min) level. To determine the representative weight of fresh needles per unit area in the forest of P. koraiensis, the tree canopy aboveground within one square meter was trimmed, and the fresh needles therein were weighed at five random points. The average weight (3980.58 g/m2) was then established as the representative weight of fresh needles per square meter. A weight of 84.03 g fresh needles were evenly laid on 2-layers absorbent gauze and fixed at the top of a 5-liter vessel (16.4 cm inside diameter), which was placed on the ground and filled with certain volume of double distilled water for simulated graded rainfall based on graded monthly precipitation. A peristaltic pump with a constant flow rate of 3770 ml/min was used to introduce the water to percolate through the packed fresh needle surface. The simulated leachates were filtered twice with qualitative filter paper via suction filtration and stored at 4 °C for quantification. |
| Sample Type: | Leaf leachate |
Treatment:
| Treatment ID: | TR003440 |
| Treatment Summary: | A volume of 100 ml of leachates was extracted by 300 ml ethyl acetate then evaporated by rotary evaporator at 45°C with 4°C-condensed water recycled in low-temperature circulator. The remaining sediment was rinsed with 2.5 ml of hexane. The liquid hexane was filtered using a 0.22 um Millipore filter for further analysis. |
Sample Preparation:
| Sampleprep ID: | SP003438 |
| Sampleprep Summary: | After rotary evaporation, the remaining sediment was washed out with 5 ml of ethyl acetate. The ethyl acetate was then filtered with a 0.22 um Millipore filter for analysis. A series concentration of endo-borneol standard were formulated in ethyl acetate to create a standard curve. |
Combined analysis:
| Analysis ID | AN005422 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 8860 GC |
| Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5977B |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH004111 |
| Chromatography Summary: | The temperature program started from an initial temperature of 50 ℃, followed by an increase of 12℃/min to 160 ℃. |
| Instrument Name: | Agilent 8860 GC |
| Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| Column Temperature: | 50 - 160 |
| Flow Gradient: | Not applicable |
| Flow Rate: | Not applicable |
| Injection Temperature: | 200 ℃ |
| Solvent A: | Not applicable |
| Solvent B: | Not applicable |
| Chromatography Type: | GC |
MS:
| MS ID: | MS005148 |
| Analysis ID: | AN005422 |
| Instrument Name: | Agilent 5977B |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | An electron ionization system with ionization energy of 7 eV was used. Helium gas (99.999%) was used as the carrier gas at constant flow rate 1 ml/min and an injection volume of 1 ul was employed. The ion-source temperature was 230 °C. The scan interval of mass spectra was 3.1 units/s and fragments from 35 to 270 da. Afterwards, Agilent MassHunter Workstation Quantitative Analysis B.07.01 were used as analytical software. The mass spectrum of the individual peak was inspected manually to confirm the peak had the correct m/z diagnostic fragments, matched with the search results from NIST MS Search 2.2 library. For endo-borneol, the target ion is m/z 95, and qualifier ions are m/z 110 and m/z 67. |
| Ion Mode: | POSITIVE |
