Summary of Study ST003333

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002072. The data can be accessed directly via it's Project DOI: 10.21228/M8SF9X This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003333
Study TitleUrine Metabolite Profiling in Indian Male Population at High Altitude using Liquid Chromatography – Mass Spectrometry: A Longitudinal Pilot Study
Study SummaryPeople who visit high altitude (HA) for work, pilgrimage, recreation purposes or deployment are subjected to environmental stresses such as low temperature, atmospheric pressure leading to hypoxia, high radiations, dry air, non-availability of fresh food and vegetables, etc. These environmental stressors pose significant physiological effects on the human body. Among these challenges, hypobaric hypoxia at high altitude affects the aerobic metabolism and thereby reducing the supply of metabolic energy. These alterations could further lead to extreme environment-related maladaptation as evidenced by changes in metabolites and metabolic pathways. In order to investigate the variation in metabolite prfile, urine samples were collected from 16 individual at base line (BL) and at High altitude (HA, 4420m). Untargeted urine metabolic profiling was performed using liquid chromatography–mass spectrometry (LC-MS) in conjunction with statistical analysis. Univariate and Multivariate statistical analysis revealed 33 metabolites based on fold change, (FC >2 and < 0.5), VIP score (>1) and p value (<0.05). These 33 metabolites were primarily associated with pathways related to Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Biotin metabolism, Cysteine and methionine metabolism, Tyrosine metabolism, Alanine, aspartate and glutamate metabolism, Pentose and glucuronate interconversions, Vitamin B6 metabolism, Citrate cycle (TCA cycle) and Porphyrin metabolism. Further, ROC analysis detected 5 metabolites i.e., 2-Tetrahydrothiopheneacetic acid (AUC: 0.97266), 1-Benzyl-7,8-dimethoxy-3-phenyl-3H-pyrazolo[3,4-c]isoquinoline (AUC: 0.92969), Abietin (AUC:0.917), 4,4'-Thiobis-2-butanone (AUC: 0.917), and Hydroxyisovaleroyl carnitine (AUC: 0.910) with a high range of sensitivity and specificity. In summary, this present longitudinal study demonstrated metabolic changes in human exposed to high altitude, via utilising the potential application of LC-MS metabolomics. These findings will shed light on the impact of hypoxic exposure on metabolic adaptation and provide a better understanding about the pathophysiological mechanism of HA related illnesses correlated to tissue hypoxia.
Institute
Defence Institute of Physiology and Allied Sciences
Last NameMeena
First NameRamesh Chand
AddressLucknow Road, Timarpur
Emailrcmeena.dipas@gov.in
Phone9868784269
Submit Date2024-07-12
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-08-08
Release Version1
Ramesh Chand Meena Ramesh Chand Meena
https://dx.doi.org/10.21228/M8SF9X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002072
Project DOI:doi: 10.21228/M8SF9X
Project Title:Urine Metabolite Profiling in Indian Male Population using Liquid Chromatography –Mass Spectrometry: A Longitudinal Pilot Study
Project Summary:People who visit high altitude (HA) for work, pilgrimage, recreation purposes or deployment are subjected to environmental stresses such as low temperature, atmospheric pressure leading to hypoxia, high radiations, dry air, non-availability of fresh food and vegetables, etc. These environmental stressors pose significant physiological effects on the human body. Among these challenges, hypobaric hypoxia at high altitude affects the aerobic metabolism and thereby reducing the supply of metabolic energy. These alterations could further lead to extreme environment-related maladaptation as evidenced by changes in metabolites and metabolic pathways. In order to investigate the variation in metabolite prfile, urine samples were collected from 16 individual at base line (BL) and at High altitude (HA, 4420m). Untargeted urine metabolic profiling was performed using liquid chromatography–mass spectrometry (LC-MS) in conjunction with statistical analysis. Univariate and Multivariate statistical analysis revealed 33 metabolites based on fold change, (FC >2 and < 0.5), VIP score (>1) and p value (<0.05). These 33 metabolites were primarily associated with pathways related to Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Biotin metabolism, Cysteine and methionine metabolism, Tyrosine metabolism, Alanine, aspartate and glutamate metabolism, Pentose and glucuronate interconversions, Vitamin B6 metabolism, Citrate cycle (TCA cycle) and Porphyrin metabolism. Further, ROC analysis detected 5 metabolites i.e., 2-Tetrahydrothiopheneacetic acid (AUC: 0.97266), 1-Benzyl-7,8-dimethoxy-3-phenyl-3H-pyrazolo[3,4-c]isoquinoline (AUC: 0.92969), Abietin (AUC:0.917), 4,4'-Thiobis-2-butanone (AUC: 0.917), and Hydroxyisovaleroyl carnitine (AUC: 0.910) with a high range of sensitivity and specificity. In summary, this present longitudinal study demonstrated metabolic changes in human exposed to high altitude, via utilising the potential application of LC-MS metabolomics. These findings will shed light on the impact of hypoxic exposure on metabolic adaptation and provide a better understanding about the pathophysiological mechanism of HA related illnesses correlated to tissue hypoxia.
Institute:Defence Institute of Physiology and Allied Sciences
Last Name:Meena
First Name:Ramesh Chand
Address:Lucknow Road, Timarpur
Email:rcmeena.dipas@gov.in
Phone:9868784269

Subject:

Subject ID:SU003454
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Altitude
SA362484DR_MEENA_DIPAS_POOL_P6Solvent Mixture QC
SA362485DR_MEENA_DIPAS_POOL_P1Solvent Mixture QC
SA362486DR_MEENA_DIPAS_POOL_P2Solvent Mixture QC
SA362487DR_MEENA_DIPAS_POOL_P4Solvent Mixture QC
SA362488DR_MEENA_DIPAS_POOL_P5Solvent Mixture QC
SA362489DR_MEENA_DIPAS_POOL_P3Solvent Mixture QC
SA362490DR_MEENA_DIPAS_POOL_N1Solvent Mixture QC
SA362491DR_MEENA_DIPAS_POOL_N2Solvent Mixture QC
SA362492DR_MEENA_DIPAS_POOL_N3Solvent Mixture QC
SA362493DR_MEENA_DIPAS_POOL_N4Solvent Mixture QC
SA362494DR_MEENA_DIPAS_POOL_N5Solvent Mixture QC
SA362495DR_MEENA_DIPAS_POOL_N6Solvent Mixture QC
SA362496DR_MEENA_DIPAS_SAMPLE_B9_PUrine Base Line
SA362497DR_MEENA_DIPAS_SAMPLE_B2_NUrine Base Line
SA362498DR_MEENA_DIPAS_SAMPLE_B16_PUrine Base Line
SA362499DR_MEENA_DIPAS_SAMPLE_B15_PUrine Base Line
SA362500DR_MEENA_DIPAS_SAMPLE_B14_PUrine Base Line
SA362501DR_MEENA_DIPAS_SAMPLE_B13_PUrine Base Line
SA362502DR_MEENA_DIPAS_SAMPLE_B12_PUrine Base Line
SA362503DR_MEENA_DIPAS_SAMPLE_B11_PUrine Base Line
SA362504DR_MEENA_DIPAS_SAMPLE_B10_PUrine Base Line
SA362505DR_MEENA_DIPAS_SAMPLE_B1_NUrine Base Line
SA362506DR_MEENA_DIPAS_SAMPLE_B8_PUrine Base Line
SA362507DR_MEENA_DIPAS_SAMPLE_B6_NUrine Base Line
SA362508DR_MEENA_DIPAS_SAMPLE_B7_PUrine Base Line
SA362509DR_MEENA_DIPAS_SAMPLE_B14_NUrine Base Line
SA362510DR_MEENA_DIPAS_SAMPLE_B13_NUrine Base Line
SA362511DR_MEENA_DIPAS_SAMPLE_B12_NUrine Base Line
SA362512DR_MEENA_DIPAS_SAMPLE_B11_NUrine Base Line
SA362513DR_MEENA_DIPAS_SAMPLE_B10_NUrine Base Line
SA362514DR_MEENA_DIPAS_SAMPLE_B9_NUrine Base Line
SA362515DR_MEENA_DIPAS_SAMPLE_B8_NUrine Base Line
SA362516DR_MEENA_DIPAS_SAMPLE_B7_NUrine Base Line
SA362517DR_MEENA_DIPAS_SAMPLE_B15_NUrine Base Line
SA362518DR_MEENA_DIPAS_SAMPLE_B5_NUrine Base Line
SA362519DR_MEENA_DIPAS_SAMPLE_B3_PUrine Base Line
SA362520DR_MEENA_DIPAS_SAMPLE_B6_PUrine Base Line
SA362521DR_MEENA_DIPAS_SAMPLE_B4_NUrine Base Line
SA362522DR_MEENA_DIPAS_SAMPLE_B4_PUrine Base Line
SA362523DR_MEENA_DIPAS_SAMPLE_B5_PUrine Base Line
SA362524DR_MEENA_DIPAS_SAMPLE_B2_PUrine Base Line
SA362525DR_MEENA_DIPAS_SAMPLE_B1_PUrine Base Line
SA362526DR_MEENA_DIPAS_SAMPLE_B16_NUrine Base Line
SA362527DR_MEENA_DIPAS_SAMPLE_B3_NUrine Base Line
SA362528DR_MEENA_DIPAS_SAMPLE_HA8_PUrine High Altitude
SA362529DR_MEENA_DIPAS_SAMPLE_HA16_PUrine High Altitude
SA362530DR_MEENA_DIPAS_SAMPLE_HA15_PUrine High Altitude
SA362531DR_MEENA_DIPAS_SAMPLE_HA14_PUrine High Altitude
SA362532DR_MEENA_DIPAS_SAMPLE_HA13_PUrine High Altitude
SA362533DR_MEENA_DIPAS_SAMPLE_HA12_PUrine High Altitude
SA362534DR_MEENA_DIPAS_SAMPLE_HA11_PUrine High Altitude
SA362535DR_MEENA_DIPAS_SAMPLE_HA10_PUrine High Altitude
SA362536DR_MEENA_DIPAS_SAMPLE_HA9_PUrine High Altitude
SA362537DR_MEENA_DIPAS_SAMPLE_HA3_PUrine High Altitude
SA362538DR_MEENA_DIPAS_SAMPLE_HA7_PUrine High Altitude
SA362539DR_MEENA_DIPAS_SAMPLE_HA10_NUrine High Altitude
SA362540DR_MEENA_DIPAS_SAMPLE_HA1_NUrine High Altitude
SA362541DR_MEENA_DIPAS_SAMPLE_HA2_NUrine High Altitude
SA362542DR_MEENA_DIPAS_SAMPLE_HA3_NUrine High Altitude
SA362543DR_MEENA_DIPAS_SAMPLE_HA4_NUrine High Altitude
SA362544DR_MEENA_DIPAS_SAMPLE_HA5_NUrine High Altitude
SA362545DR_MEENA_DIPAS_SAMPLE_HA6_NUrine High Altitude
SA362546DR_MEENA_DIPAS_SAMPLE_HA8_NUrine High Altitude
SA362547DR_MEENA_DIPAS_SAMPLE_HA9_NUrine High Altitude
SA362548DR_MEENA_DIPAS_SAMPLE_HA11_NUrine High Altitude
SA362549DR_MEENA_DIPAS_SAMPLE_HA6_PUrine High Altitude
SA362550DR_MEENA_DIPAS_SAMPLE_HA12_NUrine High Altitude
SA362551DR_MEENA_DIPAS_SAMPLE_HA13_NUrine High Altitude
SA362552DR_MEENA_DIPAS_SAMPLE_HA14_NUrine High Altitude
SA362553DR_MEENA_DIPAS_SAMPLE_HA15_NUrine High Altitude
SA362554DR_MEENA_DIPAS_SAMPLE_HA16_NUrine High Altitude
SA362555DR_MEENA_DIPAS_SAMPLE_HA1_PUrine High Altitude
SA362556DR_MEENA_DIPAS_SAMPLE_HA2_PUrine High Altitude
SA362557DR_MEENA_DIPAS_SAMPLE_HA4_PUrine High Altitude
SA362558DR_MEENA_DIPAS_SAMPLE_HA5_PUrine High Altitude
SA362559DR_MEENA_DIPAS_SAMPLE_HA7_NUrine High Altitude
Showing results 1 to 76 of 76

Collection:

Collection ID:CO003447
Collection Summary:First urine pass of the morning (120 ml), was collected in sterile urine vials. The initial sample collection took place at base line, denoted as BL (210 m). After the BL collection, participants travelled to different altitude (9000m-15000m) for acclimatization and reached at high altitude, 4420m (HA), and final collection was done after their stay for on month. Within 1 h of collection, all the samples were immediately treated with Sodium Azide (2.5mM), centrifuged for 10 min at 4000 rpm for removal of particles. The samples were immediately distributed in aliquots of 2 ml each and stored at −40 °C. The sample were then transported to BL, stored at −80 °C for further acquisition and analysis.
Sample Type:Urine
Collection Tube Temp:-80 degree

Treatment:

Treatment ID:TR003463
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP003461
Sampleprep Summary:200 μL urine sample was combined with 200 μL of acetonitrile, followed by vortexing the mixture for 30 seconds. Then the mixture underwent centrifugation at 14,000 x g for 10 minutes. After being vacuum-dried, the dried powder was dissolved with 200 uL of 2% acetonitrile.

Combined analysis:

Analysis ID AN005460 AN005461
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um) Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Orbitrap Exploris 480 Thermo Orbitrap Exploris 480
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH004147
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um)
Column Temperature:25
Flow Gradient:0 min (2% B), 0–2 min (2% B), 2–5 min (2-20% B), 5–9 min (20 – 100 % B) , 9–13 min (100% B),13-13.1 (2% B) and 13.1-16min (2% B)
Flow Rate:0.35 mL/min
Solvent A:100% Water; 0.1% formic acid
Solvent B:100% Acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS005186
Analysis ID:AN005460
Instrument Name:Thermo Orbitrap Exploris 480
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:The mass spectrometric data were collected using a Thermo Orbitrap Exploris mass spectrometer. RAW files generated were analyzed with Compound Discoverer (CD) 3.3 (Thermo Fisher Scientific, USA) software, mainly for peak extraction, peak alignment and compound identification.
Ion Mode:POSITIVE
  
MS ID:MS005187
Analysis ID:AN005461
Instrument Name:Thermo Orbitrap Exploris 480
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:The mass spectrometric data were collected using a Thermo Orbitrap Exploris mass spectrometer. RAW files generated were analyzed with Compound Discoverer (CD) 3.3 (Thermo Fisher Scientific, USA) software, mainly for peak extraction, peak alignment and compound identification.
Ion Mode:NEGATIVE
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