Summary of Study ST003363

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002089. The data can be accessed directly via it's Project DOI: 10.21228/M8KR7D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST003363
Study TitleHuman milk microbiome across lactation stages and free glutamate concentrations in healthy Ecuadorian women
Study SummaryProject Summary: To examine the differences in microbiome and glutamate concentrations in human breast milk among different lactation stages (colostrum-, transition-, and mature-milk), a longitudinal cohort study for healthy Ecuadorian women was conducted. This deposition is for the glutamate analysis.
Institute
Universidad Internacional del Ecuador
LaboratoryCollaboration with the Edison Lab at UGA
Last NameUchimiya
First NameMario
Address315 Riverbend Rd, Athens, GA, 30602, USA
Emailmario.uchimiya@uga.edu
Phone‭(706) 542-8387‬
Submit Date2024-07-29
Raw Data AvailableYes
Raw Data File Type(s)ser
Analysis Type DetailNMR
Release Date2024-08-29
Release Version1
Mario Uchimiya Mario Uchimiya
https://dx.doi.org/10.21228/M8KR7D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002089
Project DOI:doi: 10.21228/M8KR7D
Project Title:Human milk microbiome across lactation stages and free glutamate concentrations in healthy Ecuadorian women
Project Summary:Project Summary: To examine the differences in microbiome and glutamate concentrations in human breast milk among different lactation stages (colostrum-, transition-, and mature-milk), a longitudinal cohort study for healthy Ecuadorian women was conducted. This deposition is for the glutamate analysis.
Institute:University of Georgia
Last Name:Uchimiya
First Name:Mario
Address:315 Riverbend Rd, Athens, GA, 30602, USA
Email:mario.uchimiya@uga.edu
Phone:‭(706) 542-8387‬
Funding Source:International Glutamate Technical Committee (MEB); Universidad Internacional del Ecuador (MEB)
Project Comments:This is a deposition for a study led by Manuel E. Baldeon and Lizbeth Peña at the Universidad Internacional del Ecuador (MEB)
Contributors:Manuel E. Baldeon; Lizbeth Peña

Subject:

Subject ID:SU003484
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Lactation_stage gender
SA36594161-Pool1Human breast milk 0 0
SA36594268-External_Control1Human breast milk 0 0
SA36594364-Pool4Human breast milk 0 0
SA36594470-External_Control3Human breast milk 0 0
SA36594563-Pool3Human breast milk 0 0
SA36594665-Pool5Human breast milk 0 0
SA36594762-Pool2Human breast milk 0 0
SA36594869-External_Control2Human breast milk 0 0
SA36594919-BBA_69e_CHuman breast milk 1 1
SA3659503-BBA_17e_CHuman breast milk 1 1
SA3659511-BBA_10e_CHuman breast milk 1 1
SA36595214-BBA_48e_CHuman breast milk 1 1
SA36595320-BBA_70e_CHuman breast milk 1 1
SA3659545-BBA_19e_CHuman breast milk 1 1
SA36595518-BBA_67e_CHuman breast milk 1 1
SA36595613-BBA_47e_CHuman breast milk 1 1
SA3659577-BBA_24e_CHuman breast milk 1 1
SA36595816-BBA_60e_CHuman breast milk 1 1
SA36595910-BBA_43e_CHuman breast milk 1 2
SA3659608-BBA_39e_CHuman breast milk 1 2
SA36596112-BBA_45e_CHuman breast milk 1 2
SA3659629-BBA_40e_CHuman breast milk 1 2
SA36596311-BBA_44e_CHuman breast milk 1 2
SA3659646-BBA_22e_CHuman breast milk 1 2
SA36596515-BBA_55e_CHuman breast milk 1 2
SA3659664-BBA_18e_CHuman breast milk 1 2
SA3659672-BBA_16e_CHuman breast milk 1 2
SA36596817-BBA_61e_CHuman breast milk 1 2
SA36596939-BBA_69e_THuman breast milk 2 1
SA36597034-BBA_48e_THuman breast milk 2 1
SA36597125-BBA_19e_THuman breast milk 2 1
SA36597238-BBA_67e_THuman breast milk 2 1
SA36597333-BBA_47e_THuman breast milk 2 1
SA36597427-BBA_24e_THuman breast milk 2 1
SA36597523-BBA_17e_THuman breast milk 2 1
SA36597636-BBA_60e_THuman breast milk 2 1
SA36597740-BBA_70e_THuman breast milk 2 1
SA36597821-BBA_10e_THuman breast milk 2 1
SA36597932-BBA_45e_THuman breast milk 2 2
SA36598037-BBA_61e_THuman breast milk 2 2
SA36598126-BBA_22e_THuman breast milk 2 2
SA36598231-BBA_44e_THuman breast milk 2 2
SA36598322-BBA_16e_THuman breast milk 2 2
SA36598435-BBA_55e_THuman breast milk 2 2
SA36598530-BBA_43e_THuman breast milk 2 2
SA36598628-BBA_39e_THuman breast milk 2 2
SA36598729-BBA_40e_THuman breast milk 2 2
SA36598824-BBA_18e_THuman breast milk 2 2
SA36598960-BBA_70e_MHuman breast milk 3 1
SA36599058-BBA_67e_MHuman breast milk 3 1
SA36599147-BBA_24e_MHuman breast milk 3 1
SA36599241-BBA_10e_MHuman breast milk 3 1
SA36599359-BBA_69e_MHuman breast milk 3 1
SA36599453-BBA_47e_MHuman breast milk 3 1
SA36599543-BBA_17e_MHuman breast milk 3 1
SA36599656-BBA_60e_MHuman breast milk 3 1
SA36599745-BBA_19e_MHuman breast milk 3 1
SA36599854-BBA_48e_MHuman breast milk 3 1
SA36599957-BBA_61e_MHuman breast milk 3 2
SA36600048-BBA_39e_MHuman breast milk 3 2
SA36600150-BBA_43e_MHuman breast milk 3 2
SA36600244-BBA_18e_MHuman breast milk 3 2
SA36600351-BBA_44e_MHuman breast milk 3 2
SA36600449-BBA_40e_MHuman breast milk 3 2
SA36600546-BBA_22e_MHuman breast milk 3 2
SA36600642-BBA_16e_MHuman breast milk 3 2
SA36600755-BBA_55e_MHuman breast milk 3 2
SA36600852-BBA_45e_MHuman breast milk 3 2
SA36600966-Extr_Blank1n.a. 0 0
SA36601067-Extr_Blank2n.a. 0 0
SA366011Buffer phosphate blankn.a. 0 0
Showing results 1 to 71 of 71

Collection:

Collection ID:CO003477
Collection Summary:7.5 to 20 mL of milk samples were collected from mothers and kept in sterile 50-mL tubes (Sigma-Aldrich). The samples were kept cold and transported to the laboratory within the first two hours of the sampling. 5 mL of the breast milk samples were aliquoted in cryovials and stored at -80oC for glutamate analysis. Lactation stage 1 = colostrum, 2 = transition, 3 = mature.
Sample Type:Milk

Treatment:

Treatment ID:TR003493
Treatment Summary:Mothers were recruited at Hospital Gineco Obstétrico Isidro Ayora in Quito, Ecuador. The participating mothers were permanent residents and indicated their willingness to exclusively breastfeed for at least two months. Mothers were healthy, ≥18 years of age, and primiparous, and they had not consumed antibiotics or probiotics during pregnancy. Each mother had given birth to a full-term baby via vaginal delivery. A total of 20 mothers donated their colostrum- (human milk from 1-7 days postpartum), transition- (15 days postpartum), and mature-milk (30 days postpartum). For Factor_2 (gender) in Study Design, 1: participant with a female baby; 2: one with a male baby; 0: not applicable.

Sample Preparation:

Sampleprep ID:SP003491
Sampleprep Summary:Human breast milk samples were extracted using a two-phase extraction method. 2 mL of chloroform-methanol (2:1) was added to each breast milk sample and vortexed for 10 seconds. 600 µL of water was then added to each sample and the mixture was centrifuged. 800 µL of the polar phase from each sample was transferred into a 1.5mL glass tube. The samples were then dried using a speed vacuum centrifuge. The polar phase was reconstituted in 700 µL of a phosphate buffer (pH 7.4; 1M TSP). The samples were transferred to 5-mm NMR tubes for NMR analysis.
Sampleprep Protocol Filename:4_Sample_preparation_protocol_Glu_2024.pdf
Processing Storage Conditions:4℃

Analysis:

Analysis ID:AN005509
Analysis Type:NMR
Analysis Protocol File:5_Analysis_protocol_Glu_2024.pdf
Num Factors:8
Num Metabolites:1
Units:NMR peak intensity

NMR:

NMR ID:NM000288
Analysis ID:AN005509
Instrument Name:Bruker AVANCE lll
Instrument Type:FT-NMR
NMR Experiment Type:2D-1H-1H
NMR Comments:The raw NMR data files are in the 'Spectra' folder.
Spectrometer Frequency:600
  logo