Summary of Study ST003389

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002100. The data can be accessed directly via it's Project DOI: 10.21228/M85J80 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003389
Study TitleDisruption of Glucose Homeostasis by Bacterial Infection Orchestrates Host Innate Immunity Through NAD+/NADH Balance
Study SummaryMetabolic reprogramming is crucial for activating innate immunity in macrophages, and the accumulation of immunometabolites is thought essential for effective defense against infection. The NAD+/NADH redox couple serves as a critical node that integrates metabolic pathways and signaling events, but how this metabolite couple engages macrophage activation remains unclear. Here, we showed that the NAD+/NADH ratio serves as a molecular signal that regulates proinflammatory responses and type I interferon (IFN) responses divergently. Salmonella Typhimurium infection led to a decreased NAD+/NADH ratio by inducing the accumulation of NADH. Further investigation showed that an increased NAD+/NADH ratio correlates to attenuated proinflammatory responses and enhanced type I IFN responses. Conversely, a decreased NAD+/NADH ratio is linked to intensified proinflammatory responses and restrained type I IFN responses. These results showed the NAD+/NADH ratio is an essential cell-intrinsic factor that orchestrates innate immunity, which enhanced our understanding of how metabolites fine-tune innate immunity.
Institute
Northwest A&F University
Last NameXu
First NameLei
AddressYangling, Xinong Road, Xiyang, 712100, China
Emailxulei@nwafu.edu.cn
Phone18149321513
Submit Date2024-07-27
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-08-09
Release Version1
Lei Xu Lei Xu
https://dx.doi.org/10.21228/M85J80
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002100
Project DOI:doi: 10.21228/M85J80
Project Title:The metabolome analysis of energy metabolites after Salmonella Typhimurium infection in mouse macrophages
Project Type:The metabolome analysis of energy metabolites
Project Summary:The metabolome analysis of energy metabolites after Salmonella Typhimurium infection in mouse macrophages. Glucose metabolites concentrations in S. Typhimurium-infected (MOI = 10, 6 h) and Mock-infected mouse Peritoneal macrophages (PM). PMs were also pretreated with 2-DG (10 mM, 18 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected. PMs were also pretreated with Rotenone (2.5 μM, 4 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected.
Institute:Northwest A&F University
Last Name:Xu
First Name:Lei
Address:Yangling, Xinong Road, Xianyang, 712100, China
Email:xulei@nwafu.edu.cn
Phone:18149321513

Subject:

Subject ID:SU003511
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals
  
Subject ID:SU003512
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA367989STPM72DG+Salmonella Typhimurium infection1
SA368001STPM72DG+Salmonella Typhimurium infection1
SA367990STPM82DG+Salmonella Typhimurium infection2
SA368002STPM82DG+Salmonella Typhimurium infection2
SA367991STPM92DG+Salmonella Typhimurium infection3
SA368003STPM92DG+Salmonella Typhimurium infection3
SA367992STPM1Control1
SA368004STPM1Control1
SA367993STPM2Control2
SA368005STPM2Control2
SA367994STPM3Control3
SA368006STPM3Control3
SA367995STPM10Rotenone+Salmonella Typhimurium infection1
SA368007STPM10Rotenone+Salmonella Typhimurium infection1
SA367996STPM11Rotenone+Salmonella Typhimurium infection2
SA368008STPM11Rotenone+Salmonella Typhimurium infection2
SA367997STPM12Rotenone+Salmonella Typhimurium infection3
SA368009STPM12Rotenone+Salmonella Typhimurium infection3
SA367998STPM4Salmonella Typhimurium infection1
SA368010STPM4Salmonella Typhimurium infection1
SA367999STPM5Salmonella Typhimurium infection2
SA368011STPM5Salmonella Typhimurium infection2
SA368000STPM6Salmonella Typhimurium infection3
SA368012STPM6Salmonella Typhimurium infection3
Showing results 1 to 24 of 24

Collection:

Collection ID:CO003504
Collection Summary:Cells in plates were counted, washed with cold PBS and then flash-frozen in liquid nitrogen
Sample Type:Macrophages
  
Collection ID:CO003505
Collection Summary:Cells in plates were counted, washed with cold PBS and then flash-frozen in liquid nitrogen
Sample Type:Macrophages

Treatment:

Treatment ID:TR003520
Treatment Summary:The metabolome analysis of energy metabolites after Salmonella Typhimurium infection in mouse macrophages. Glucose metabolites concentrations in S. Typhimurium-infected (MOI = 10, 6 h) and Mock-infected mouse Peritoneal macrophages. PMs were also pretreated with 2-DG (10 mM, 18 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected. PMs were also pretreated with Rotenone (2.5 μM, 4 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected.
  
Treatment ID:TR003521
Treatment Summary:The metabolome analysis of energy metabolites after Salmonella Typhimurium infection in mouse macrophages. Glucose metabolites concentrations in S. Typhimurium-infected (MOI = 10, 6 h) and Mock-infected mouse Peritoneal macrophages. PMs were also pretreated with 2-DG (10 mM, 18 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected. PMs were also pretreated with Rotenone (2.5 μM, 4 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected.

Sample Preparation:

Sampleprep ID:SP003518
Sampleprep Summary:2 × 107 cells were harvested and thawed at 4°C mixed with 200 μL water, and 800 μL of cold methanol/acetonitrile (1:1, v/v) to remove the protein. The mixture was centrifuged for 15 min (14000 × g, 4°C). The supernatant was dried in a vacuum centrifuge.
  
Sampleprep ID:SP003519
Sampleprep Summary:2 × 107 cells were harvested and thawed at 4°C mixed with 200 μL water, and 800 μL of cold methanol/acetonitrile (1:1, v/v) to remove the protein. The mixture was centrifuged for 15 min (14000 × g, 4°C). The supernatant was dried in a vacuum centrifuge.

Combined analysis:

Analysis ID AN005557 AN005558
Chromatography ID CH004221 CH004222
MS ID MS005282 MS005283
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity
Column Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap
Ion Mode NEGATIVE NEGATIVE
Units μmol/g umol/g

Chromatography:

Chromatography ID:CH004221
Instrument Name:Agilent 1290 Infinity
Column Name:Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:35°C
Flow Gradient:0-18 min: 90% B to 40% B, 18-18.1 min: 40% B to 90% B, 18.1-23 min: 90% B.
Flow Rate:300 μL/min
Solvent A:100% water; 15 mM ammonium acetate
Solvent B:100% acetonitrile
Chromatography Type:HILIC
  
Chromatography ID:CH004222
Instrument Name:Agilent 1290 Infinity
Column Name:Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:35°C
Flow Gradient:0-18 min: 90% B to 40% B, 18-18.1 min: 40% B to 90% B, 18.1-23 min: 90% B.
Flow Rate:300 μL/min
Solvent A:100% water; 15 mM ammonium acetate
Solvent B:100% acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS005282
Analysis ID:AN005557
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:sheath gas temperature: 350°C; ion Source Gas1 (Gas1): 45 psi, Ion Source Gas2 (Gas2): 45 psi, Curtain gas (CUR): 30 psi; IonSpray Voltage Floating (ISVF): 4500 V; nebulizer pressure: 40psi. The mass spectrometer operated with a dwell time of 200 ms.
Ion Mode:NEGATIVE
  
MS ID:MS005283
Analysis ID:AN005558
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:sheath gas temperature: 350°C; ion Source Gas1 (Gas1): 45 psi, Ion Source Gas2 (Gas2): 45 psi, Curtain gas (CUR): 30 psi; IonSpray Voltage Floating (ISVF): 4500 V; nebulizer pressure: 40psi. The mass spectrometer operated with a dwell time of 200 ms.
Ion Mode:NEGATIVE
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