Summary of Study ST003389
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002100. The data can be accessed directly via it's Project DOI: 10.21228/M85J80 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003389 |
| Study Title | Disruption of Glucose Homeostasis by Bacterial Infection Orchestrates Host Innate Immunity Through NAD+/NADH Balance |
| Study Summary | Metabolic reprogramming is crucial for activating innate immunity in macrophages, and the accumulation of immunometabolites is thought essential for effective defense against infection. The NAD+/NADH redox couple serves as a critical node that integrates metabolic pathways and signaling events, but how this metabolite couple engages macrophage activation remains unclear. Here, we showed that the NAD+/NADH ratio serves as a molecular signal that regulates proinflammatory responses and type I interferon (IFN) responses divergently. Salmonella Typhimurium infection led to a decreased NAD+/NADH ratio by inducing the accumulation of NADH. Further investigation showed that an increased NAD+/NADH ratio correlates to attenuated proinflammatory responses and enhanced type I IFN responses. Conversely, a decreased NAD+/NADH ratio is linked to intensified proinflammatory responses and restrained type I IFN responses. These results showed the NAD+/NADH ratio is an essential cell-intrinsic factor that orchestrates innate immunity, which enhanced our understanding of how metabolites fine-tune innate immunity. |
| Institute | Northwest A&F University |
| Last Name | Xu |
| First Name | Lei |
| Address | Yangling, Xinong Road, Xiyang, 712100, China |
| xulei@nwafu.edu.cn | |
| Phone | 18149321513 |
| Submit Date | 2024-07-27 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-08-09 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002100 |
| Project DOI: | doi: 10.21228/M85J80 |
| Project Title: | The metabolome analysis of energy metabolites after Salmonella Typhimurium infection in mouse macrophages |
| Project Type: | The metabolome analysis of energy metabolites |
| Project Summary: | The metabolome analysis of energy metabolites after Salmonella Typhimurium infection in mouse macrophages. Glucose metabolites concentrations in S. Typhimurium-infected (MOI = 10, 6 h) and Mock-infected mouse Peritoneal macrophages (PM). PMs were also pretreated with 2-DG (10 mM, 18 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected. PMs were also pretreated with Rotenone (2.5 μM, 4 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected. |
| Institute: | Northwest A&F University |
| Last Name: | Xu |
| First Name: | Lei |
| Address: | Yangling, Xinong Road, Xianyang, 712100, China |
| Email: | xulei@nwafu.edu.cn |
| Phone: | 18149321513 |
Subject:
| Subject ID: | SU003511 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Subject ID: | SU003512 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Factor |
|---|---|---|---|
| SA367989 | STPM7 | Peritoneal macrophages | 2DG+Salmonella Typhimurium infection1 |
| SA368001 | STPM7 | Peritoneal macrophages | 2DG+Salmonella Typhimurium infection1 |
| SA367990 | STPM8 | Peritoneal macrophages | 2DG+Salmonella Typhimurium infection2 |
| SA368002 | STPM8 | Peritoneal macrophages | 2DG+Salmonella Typhimurium infection2 |
| SA367991 | STPM9 | Peritoneal macrophages | 2DG+Salmonella Typhimurium infection3 |
| SA368003 | STPM9 | Peritoneal macrophages | 2DG+Salmonella Typhimurium infection3 |
| SA367992 | STPM1 | Peritoneal macrophages | Control1 |
| SA368004 | STPM1 | Peritoneal macrophages | Control1 |
| SA367993 | STPM2 | Peritoneal macrophages | Control2 |
| SA368005 | STPM2 | Peritoneal macrophages | Control2 |
| SA367994 | STPM3 | Peritoneal macrophages | Control3 |
| SA368006 | STPM3 | Peritoneal macrophages | Control3 |
| SA367995 | STPM10 | Peritoneal macrophages | Rotenone+Salmonella Typhimurium infection1 |
| SA368007 | STPM10 | Peritoneal macrophages | Rotenone+Salmonella Typhimurium infection1 |
| SA367996 | STPM11 | Peritoneal macrophages | Rotenone+Salmonella Typhimurium infection2 |
| SA368008 | STPM11 | Peritoneal macrophages | Rotenone+Salmonella Typhimurium infection2 |
| SA367997 | STPM12 | Peritoneal macrophages | Rotenone+Salmonella Typhimurium infection3 |
| SA368009 | STPM12 | Peritoneal macrophages | Rotenone+Salmonella Typhimurium infection3 |
| SA367998 | STPM4 | Peritoneal macrophages | Salmonella Typhimurium infection1 |
| SA368010 | STPM4 | Peritoneal macrophages | Salmonella Typhimurium infection1 |
| SA367999 | STPM5 | Peritoneal macrophages | Salmonella Typhimurium infection2 |
| SA368011 | STPM5 | Peritoneal macrophages | Salmonella Typhimurium infection2 |
| SA368000 | STPM6 | Peritoneal macrophages | Salmonella Typhimurium infection3 |
| SA368012 | STPM6 | Peritoneal macrophages | Salmonella Typhimurium infection3 |
| Showing results 1 to 24 of 24 |
Collection:
| Collection ID: | CO003504 |
| Collection Summary: | Cells in plates were counted, washed with cold PBS and then flash-frozen in liquid nitrogen |
| Sample Type: | Macrophages |
| Collection ID: | CO003505 |
| Collection Summary: | Cells in plates were counted, washed with cold PBS and then flash-frozen in liquid nitrogen |
| Sample Type: | Macrophages |
Treatment:
| Treatment ID: | TR003520 |
| Treatment Summary: | The metabolome analysis of energy metabolites after Salmonella Typhimurium infection in mouse macrophages. Glucose metabolites concentrations in S. Typhimurium-infected (MOI = 10, 6 h) and Mock-infected mouse Peritoneal macrophages. PMs were also pretreated with 2-DG (10 mM, 18 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected. PMs were also pretreated with Rotenone (2.5 μM, 4 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected. |
| Treatment ID: | TR003521 |
| Treatment Summary: | The metabolome analysis of energy metabolites after Salmonella Typhimurium infection in mouse macrophages. Glucose metabolites concentrations in S. Typhimurium-infected (MOI = 10, 6 h) and Mock-infected mouse Peritoneal macrophages. PMs were also pretreated with 2-DG (10 mM, 18 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected. PMs were also pretreated with Rotenone (2.5 μM, 4 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected. |
Sample Preparation:
| Sampleprep ID: | SP003518 |
| Sampleprep Summary: | 2 × 107 cells were harvested and thawed at 4°C mixed with 200 μL water, and 800 μL of cold methanol/acetonitrile (1:1, v/v) to remove the protein. The mixture was centrifuged for 15 min (14000 × g, 4°C). The supernatant was dried in a vacuum centrifuge. |
| Sampleprep ID: | SP003519 |
| Sampleprep Summary: | 2 × 107 cells were harvested and thawed at 4°C mixed with 200 μL water, and 800 μL of cold methanol/acetonitrile (1:1, v/v) to remove the protein. The mixture was centrifuged for 15 min (14000 × g, 4°C). The supernatant was dried in a vacuum centrifuge. |
Combined analysis:
| Analysis ID | AN005557 | AN005558 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | NEGATIVE | NEGATIVE |
| Units | μmol/g | umol/g |
Chromatography:
| Chromatography ID: | CH004221 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 35°C |
| Flow Gradient: | 0-18 min: 90% B to 40% B, 18-18.1 min: 40% B to 90% B, 18.1-23 min: 90% B. |
| Flow Rate: | 300 μL/min |
| Solvent A: | 100% water; 15 mM ammonium acetate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH004222 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 35°C |
| Flow Gradient: | 0-18 min: 90% B to 40% B, 18-18.1 min: 40% B to 90% B, 18.1-23 min: 90% B. |
| Flow Rate: | 300 μL/min |
| Solvent A: | 100% water; 15 mM ammonium acetate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS005282 |
| Analysis ID: | AN005557 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | sheath gas temperature: 350°C; ion Source Gas1 (Gas1): 45 psi, Ion Source Gas2 (Gas2): 45 psi, Curtain gas (CUR): 30 psi; IonSpray Voltage Floating (ISVF): 4500 V; nebulizer pressure: 40psi. The mass spectrometer operated with a dwell time of 200 ms. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS005283 |
| Analysis ID: | AN005558 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | sheath gas temperature: 350°C; ion Source Gas1 (Gas1): 45 psi, Ion Source Gas2 (Gas2): 45 psi, Curtain gas (CUR): 30 psi; IonSpray Voltage Floating (ISVF): 4500 V; nebulizer pressure: 40psi. The mass spectrometer operated with a dwell time of 200 ms. |
| Ion Mode: | NEGATIVE |
