Summary of Study ST003389

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002100. The data can be accessed directly via it's Project DOI: 10.21228/M85J80 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003389
Study TitleDisruption of Glucose Homeostasis by Bacterial Infection Orchestrates Host Innate Immunity Through NAD+/NADH Balance
Study SummaryMetabolic reprogramming is crucial for activating innate immunity in macrophages, and the accumulation of immunometabolites is thought essential for effective defense against infection. The NAD+/NADH redox couple serves as a critical node that integrates metabolic pathways and signaling events, but how this metabolite couple engages macrophage activation remains unclear. Here, we showed that the NAD+/NADH ratio serves as a molecular signal that regulates proinflammatory responses and type I interferon (IFN) responses divergently. Salmonella Typhimurium infection led to a decreased NAD+/NADH ratio by inducing the accumulation of NADH. Further investigation showed that an increased NAD+/NADH ratio correlates to attenuated proinflammatory responses and enhanced type I IFN responses. Conversely, a decreased NAD+/NADH ratio is linked to intensified proinflammatory responses and restrained type I IFN responses. These results showed the NAD+/NADH ratio is an essential cell-intrinsic factor that orchestrates innate immunity, which enhanced our understanding of how metabolites fine-tune innate immunity.
Institute
Northwest A&F University
Last NameXu
First NameLei
AddressYangling, Xinong Road, Xiyang, 712100, China
Emailxulei@nwafu.edu.cn
Phone18149321513
Submit Date2024-07-27
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-08-09
Release Version1
Lei Xu Lei Xu
https://dx.doi.org/10.21228/M85J80
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002100
Project DOI:doi: 10.21228/M85J80
Project Title:The metabolome analysis of energy metabolites after Salmonella Typhimurium infection in mouse macrophages
Project Type:The metabolome analysis of energy metabolites
Project Summary:The metabolome analysis of energy metabolites after Salmonella Typhimurium infection in mouse macrophages. Glucose metabolites concentrations in S. Typhimurium-infected (MOI = 10, 6 h) and Mock-infected mouse Peritoneal macrophages (PM). PMs were also pretreated with 2-DG (10 mM, 18 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected. PMs were also pretreated with Rotenone (2.5 μM, 4 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected.
Institute:Northwest A&F University
Last Name:Xu
First Name:Lei
Address:Yangling, Xinong Road, Xianyang, 712100, China
Email:xulei@nwafu.edu.cn
Phone:18149321513

Subject:

Subject ID:SU003511
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals
  
Subject ID:SU003512
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA367989STPM72DG+Salmonella Typhimurium infection1
SA368001STPM72DG+Salmonella Typhimurium infection1
SA367990STPM82DG+Salmonella Typhimurium infection2
SA368002STPM82DG+Salmonella Typhimurium infection2
SA367991STPM92DG+Salmonella Typhimurium infection3
SA368003STPM92DG+Salmonella Typhimurium infection3
SA367992STPM1Control1
SA368004STPM1Control1
SA367993STPM2Control2
SA368005STPM2Control2
SA367994STPM3Control3
SA368006STPM3Control3
SA367995STPM10Rotenone+Salmonella Typhimurium infection1
SA368007STPM10Rotenone+Salmonella Typhimurium infection1
SA367996STPM11Rotenone+Salmonella Typhimurium infection2
SA368008STPM11Rotenone+Salmonella Typhimurium infection2
SA367997STPM12Rotenone+Salmonella Typhimurium infection3
SA368009STPM12Rotenone+Salmonella Typhimurium infection3
SA367998STPM4Salmonella Typhimurium infection1
SA368010STPM4Salmonella Typhimurium infection1
SA367999STPM5Salmonella Typhimurium infection2
SA368011STPM5Salmonella Typhimurium infection2
SA368000STPM6Salmonella Typhimurium infection3
SA368012STPM6Salmonella Typhimurium infection3
Showing results 1 to 24 of 24

Collection:

Collection ID:CO003504
Collection Summary:Cells in plates were counted, washed with cold PBS and then flash-frozen in liquid nitrogen
Sample Type:Macrophages
  
Collection ID:CO003505
Collection Summary:Cells in plates were counted, washed with cold PBS and then flash-frozen in liquid nitrogen
Sample Type:Macrophages

Treatment:

Treatment ID:TR003520
Treatment Summary:The metabolome analysis of energy metabolites after Salmonella Typhimurium infection in mouse macrophages. Glucose metabolites concentrations in S. Typhimurium-infected (MOI = 10, 6 h) and Mock-infected mouse Peritoneal macrophages. PMs were also pretreated with 2-DG (10 mM, 18 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected. PMs were also pretreated with Rotenone (2.5 μM, 4 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected.
  
Treatment ID:TR003521
Treatment Summary:The metabolome analysis of energy metabolites after Salmonella Typhimurium infection in mouse macrophages. Glucose metabolites concentrations in S. Typhimurium-infected (MOI = 10, 6 h) and Mock-infected mouse Peritoneal macrophages. PMs were also pretreated with 2-DG (10 mM, 18 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected. PMs were also pretreated with Rotenone (2.5 μM, 4 h) and infected with S. Typhimurium (MOI =10, 6 h) or Mock-infected.

Sample Preparation:

Sampleprep ID:SP003518
Sampleprep Summary:2 × 107 cells were harvested and thawed at 4°C mixed with 200 μL water, and 800 μL of cold methanol/acetonitrile (1:1, v/v) to remove the protein. The mixture was centrifuged for 15 min (14000 × g, 4°C). The supernatant was dried in a vacuum centrifuge.
  
Sampleprep ID:SP003519
Sampleprep Summary:2 × 107 cells were harvested and thawed at 4°C mixed with 200 μL water, and 800 μL of cold methanol/acetonitrile (1:1, v/v) to remove the protein. The mixture was centrifuged for 15 min (14000 × g, 4°C). The supernatant was dried in a vacuum centrifuge.

Chromatography:

Chromatography ID:CH004221
Instrument Name:Agilent 1290 Infinity
Column Name:Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:35°C
Flow Gradient:0-18 min: 90% B to 40% B, 18-18.1 min: 40% B to 90% B, 18.1-23 min: 90% B.
Flow Rate:300 μL/min
Solvent A:100% water; 15 mM ammonium acetate
Solvent B:100% acetonitrile
Chromatography Type:HILIC
  
Chromatography ID:CH004222
Instrument Name:Agilent 1290 Infinity
Column Name:Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:35°C
Flow Gradient:0-18 min: 90% B to 40% B, 18-18.1 min: 40% B to 90% B, 18.1-23 min: 90% B.
Flow Rate:300 μL/min
Solvent A:100% water; 15 mM ammonium acetate
Solvent B:100% acetonitrile
Chromatography Type:HILIC

Analysis:

Analysis ID:AN005557
Analysis Type:MS
Chromatography ID:CH004221
Num Factors:12
Num Metabolites:21
Rt Units:Minutes
Units:μmol/g
  
Analysis ID:AN005558
Analysis Type:MS
Chromatography ID:CH004222
Num Factors:12
Num Metabolites:21
Rt Units:Minutes
Units:umol/g
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