Summary of Study ST003400

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002106. The data can be accessed directly via it's Project DOI: 10.21228/M8D256 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples | Perform analysis on untargeted data
Download mwTab file (text) | Download mwTab file(JSON) | Download data files (Contains raw data)

Study ID	ST003400
Study Title	A Study on the Mechanism of Perinatal BPS Exposure Promoting Obesity Based on Metabolomics and Microbiomics
Study Summary	Due to increasingly stringent regulations on bisphenol A (BPA) usage, its structurally similar counterpart, BPS, has become widely employed as the primary substitute in various industries such as food packaging. However, recent years have unveiled potential risks of obesity promotion and insulin resistance associated with BPS, particularly during early life stages, although its precise impact remains inadequately understood. Addressing these concerns, this study established a mouse model to investigate the effects of maternal BPS exposure during pregnancy and lactation, combined with offspring consumption of a high-fat diet. The research examined physiological indicators related to obesity and insulin resistance in offspring, evaluated pathological changes in vital organs including the heart, liver, pancreas, white adipose tissue (WAT), and brown adipose tissue (BAT), conducted metabolomics perturbation analysis across multiple organs, and performed microbiome analysis based on fecal samples. Finally, correlations between phenotype, metabolome, and microbiome were explored to unravel intergenerational effects and mechanisms under BPS exposure, aiming to identify potential biomarkers of its effects.
Institute	College of Marine Food and Biological Engineering, Jimei University
Last Name	Li
First Name	Shuyin
Address	No.185 Yinjiang Road,Jimei District,Xiamen City,Fujian Province,China
Email	164000107@qq.com
Phone	18750682266
Submit Date	2024-08-02
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2024-09-03
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR002106
Project DOI:	doi: 10.21228/M8D256
Project Title:	A Study on the Mechanism of Perinatal BPS Exposure Promoting Obesity Based on Metabolomics and Microbiomics
Project Summary:	Due to increasingly stringent regulations on bisphenol A (BPA) usage, its structurally similar counterpart, BPS, has become widely employed as the primary substitute in various industries such as food packaging. However, recent years have unveiled potential risks of obesity promotion and insulin resistance associated with BPS, particularly during early life stages, although its precise impact remains inadequately understood. Addressing these concerns, this study established a mouse model to investigate the effects of maternal BPS exposure during pregnancy and lactation, combined with offspring consumption of a high-fat diet. The research examined physiological indicators related to obesity and insulin resistance in offspring, evaluated pathological changes in vital organs including the heart, liver, pancreas, white adipose tissue, and brown adipose tissue, conducted metabolomics perturbation analysis across multiple organs, and performed microbiome analysis based on fecal samples. Finally, correlations between phenotype, metabolome, and microbiome were explored to unravel intergenerational effects and mechanisms under BPS exposure, aiming to identify potential biomarkers of its effects.
Institute:	College of Marine Food and Biological Engineering, Jimei University
Last Name:	Li
First Name:	Shuyin
Address:	No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, China
Email:	164000107@qq.com
Phone:	18750682266

Subject:

Subject ID:	SU003525
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	BPS Treatment	Sample source
SA371592	BLF-267	0.05mg/kg/d	Brown Adipose Tissue
SA371593	BLF-258	0.05mg/kg/d	Brown Adipose Tissue
SA371594	BLF-261	0.05mg/kg/d	Brown Adipose Tissue
SA371595	BLF-262	0.05mg/kg/d	Brown Adipose Tissue
SA371596	BLF-263	0.05mg/kg/d	Brown Adipose Tissue
SA371597	BLF-266	0.05mg/kg/d	Brown Adipose Tissue
SA371598	BLF-264	0.05mg/kg/d	Brown Adipose Tissue
SA371599	BLM-247	0.05mg/kg/d	Brown Adipose Tissue
SA371600	BLM-252	0.05mg/kg/d	Brown Adipose Tissue
SA371601	BLM-253	0.05mg/kg/d	Brown Adipose Tissue
SA371602	BLM-255	0.05mg/kg/d	Brown Adipose Tissue
SA371603	BLM-275	0.05mg/kg/d	Brown Adipose Tissue
SA371604	BLM-278	0.05mg/kg/d	Brown Adipose Tissue
SA371605	BLM-279	0.05mg/kg/d	Brown Adipose Tissue
SA371606	HLM-247	0.05mg/kg/d	Heart Tissue
SA371607	HLF-258	0.05mg/kg/d	Heart Tissue
SA371608	HLF-261	0.05mg/kg/d	Heart Tissue
SA371609	HLF-262	0.05mg/kg/d	Heart Tissue
SA371610	HLF-264	0.05mg/kg/d	Heart Tissue
SA371611	HLF-266	0.05mg/kg/d	Heart Tissue
SA371612	HLF-267	0.05mg/kg/d	Heart Tissue
SA371613	HLF-263	0.05mg/kg/d	Heart Tissue
SA371614	HLM-252	0.05mg/kg/d	Heart Tissue
SA371615	HLM-255	0.05mg/kg/d	Heart Tissue
SA371616	HLM-275	0.05mg/kg/d	Heart Tissue
SA371617	HLM-278	0.05mg/kg/d	Heart Tissue
SA371618	HLM-279	0.05mg/kg/d	Heart Tissue
SA371619	HLM-253	0.05mg/kg/d	Heart Tissue
SA371620	LLM-247	0.05mg/kg/d	Liver Tissue
SA371621	LLM-279	0.05mg/kg/d	Liver Tissue
SA371622	LLM-255	0.05mg/kg/d	Liver Tissue
SA371623	LLM-253	0.05mg/kg/d	Liver Tissue
SA371624	LLM-252	0.05mg/kg/d	Liver Tissue
SA371625	LLF-264	0.05mg/kg/d	Liver Tissue
SA371626	LLF-267	0.05mg/kg/d	Liver Tissue
SA371627	LLF-266	0.05mg/kg/d	Liver Tissue
SA371628	LLM-275	0.05mg/kg/d	Liver Tissue
SA371629	LLF-263	0.05mg/kg/d	Liver Tissue
SA371630	LLF-262	0.05mg/kg/d	Liver Tissue
SA371631	LLF-261	0.05mg/kg/d	Liver Tissue
SA371632	LLF-258	0.05mg/kg/d	Liver Tissue
SA371633	LLM-278	0.05mg/kg/d	Liver Tissue
SA371634	PLM-255	0.05mg/kg/d	Pancreas Tissue
SA371635	PLM-275	0.05mg/kg/d	Pancreas Tissue
SA371636	PLM-253	0.05mg/kg/d	Pancreas Tissue
SA371637	PLM-252	0.05mg/kg/d	Pancreas Tissue
SA371638	PLM-247	0.05mg/kg/d	Pancreas Tissue
SA371639	PLF-267	0.05mg/kg/d	Pancreas Tissue
SA371640	PLF-266	0.05mg/kg/d	Pancreas Tissue
SA371641	PLF-264	0.05mg/kg/d	Pancreas Tissue
SA371642	PLF-263	0.05mg/kg/d	Pancreas Tissue
SA371643	PLF-258	0.05mg/kg/d	Pancreas Tissue
SA371644	PLF-261	0.05mg/kg/d	Pancreas Tissue
SA371645	PLM-279	0.05mg/kg/d	Pancreas Tissue
SA371646	PLM-278	0.05mg/kg/d	Pancreas Tissue
SA371647	PLF-262	0.05mg/kg/d	Pancreas Tissue
SA371648	WLF-258	0.05mg/kg/d	White Adipose Tissue
SA371649	WLM-247	0.05mg/kg/d	White Adipose Tissue
SA371650	WLF-262	0.05mg/kg/d	White Adipose Tissue
SA371651	WLF-263	0.05mg/kg/d	White Adipose Tissue
SA371652	WLF-264	0.05mg/kg/d	White Adipose Tissue
SA371653	WLF-266	0.05mg/kg/d	White Adipose Tissue
SA371654	WLF-267	0.05mg/kg/d	White Adipose Tissue
SA371655	WLF-261	0.05mg/kg/d	White Adipose Tissue
SA371656	WLM-252	0.05mg/kg/d	White Adipose Tissue
SA371657	WLM-255	0.05mg/kg/d	White Adipose Tissue
SA371658	WLM-275	0.05mg/kg/d	White Adipose Tissue
SA371659	WLM-278	0.05mg/kg/d	White Adipose Tissue
SA371660	WLM-279	0.05mg/kg/d	White Adipose Tissue
SA371661	WLM-253	0.05mg/kg/d	White Adipose Tissue
SA371662	BHF-4417	5mg/kg/d	Brown Adipose Tissue
SA371663	BHM-294	5mg/kg/d	Brown Adipose Tissue
SA371664	BHM-290	5mg/kg/d	Brown Adipose Tissue
SA371665	BHM-289	5mg/kg/d	Brown Adipose Tissue
SA371666	BHF-4438	5mg/kg/d	Brown Adipose Tissue
SA371667	BHF-4419	5mg/kg/d	Brown Adipose Tissue
SA371668	BHF-4418	5mg/kg/d	Brown Adipose Tissue
SA371669	BHF-307	5mg/kg/d	Brown Adipose Tissue
SA371670	BHF-4416	5mg/kg/d	Brown Adipose Tissue
SA371671	BHF-4415	5mg/kg/d	Brown Adipose Tissue
SA371672	BHF-4413	5mg/kg/d	Brown Adipose Tissue
SA371673	BHF-306	5mg/kg/d	Brown Adipose Tissue
SA371674	BHF-299	5mg/kg/d	Brown Adipose Tissue
SA371675	BHF-298	5mg/kg/d	Brown Adipose Tissue
SA371676	BHM-301	5mg/kg/d	Brown Adipose Tissue
SA371677	BHF-295	5mg/kg/d	Brown Adipose Tissue
SA371678	BHF-297	5mg/kg/d	Brown Adipose Tissue
SA371679	BHM-300	5mg/kg/d	Brown Adipose Tissue
SA371680	BHM-305	5mg/kg/d	Brown Adipose Tissue
SA371681	BHM-4452	5mg/kg/d	Brown Adipose Tissue
SA371682	BHM-4434	5mg/kg/d	Brown Adipose Tissue
SA371683	BHM-4433	5mg/kg/d	Brown Adipose Tissue
SA371684	BHM-4432	5mg/kg/d	Brown Adipose Tissue
SA371685	BHM-4431	5mg/kg/d	Brown Adipose Tissue
SA371686	BHM-4427	5mg/kg/d	Brown Adipose Tissue
SA371687	BHM-4423	5mg/kg/d	Brown Adipose Tissue
SA371688	BHM-304	5mg/kg/d	Brown Adipose Tissue
SA371689	HHF-299	5mg/kg/d	Heart Tissue
SA371690	HHF-307	5mg/kg/d	Heart Tissue
SA371691	HHF-306	5mg/kg/d	Heart Tissue

Showing page 1 of 3 Results: 1 2 3 Next Showing results 1 to 100 of 275

Collection:

Collection ID:	CO003518
Collection Summary:	After 3 weeks of breastfeeding, F1 mice were fed adaptive diet for 1 week, followed by high-fat diet for 8 weeks.After mouse feces were collected,the method of euthanizing mice by cervical dislocation. After sampling, Mouse heart, liver, pancreas, white adipose and brown adipose were collected. After sampling, the tissues were quickly washed in normal saline. After cleaning, the tissues were treated with liquid nitrogen and finally frozen in the refrigerator at -80 ℃.
Sample Type:	White adipose,Brown adipose,Heart,Liver,Pancreas

Treatment:

Treatment ID:	TR003534
Treatment Summary:	Briefly,C57BL/6 pregnant mice were fed ordinary diet under constant temperature [(23±1) ℃] and constant humidity [(55±5)%], and followed a strict 12 h/12 h light/dark cycle.Animals were kept in polystyrene rat cages for the experiment. They were divided into normal saline solvent as control, low-dose BPS group (0.05 mg/kg/d) and high-dose BPS group (5 mg/kg/d). BPS exposure was performed by subcutaneous injection. The general state of pregnant mice was observed daily, and their body mass was weighed once a week. After delivery, pregnant rats were exposed to BPS in different groups during lactation period, and the mothers were killed after lactation period. After lactation, F1 mice were observed in general state every day and weighed once a week. After 3 weeks of breastfeeding, F1 mice were fed adaptive diet for 1 week, followed by high-fat diet for 8 weeks. Mouse feces were collected, and mouse heart, liver, pancreas, white adipose and brown adipose were collected. After sampling, the mice were quickly washed in normal saline. After cleaning, the mice were treated with liquid nitrogen and finally frozen in the refrigerator at -80 ℃.

Treatment ID:

TR003534

Treatment Summary:

Briefly,C57BL/6 pregnant mice were fed ordinary diet under constant temperature [(23±1) ℃] and constant humidity [(55±5)%], and followed a strict 12 h/12 h light/dark cycle.Animals were kept in polystyrene rat cages for the experiment. They were divided into normal saline solvent as control, low-dose BPS group (0.05 mg/kg/d) and high-dose BPS group (5 mg/kg/d). BPS exposure was performed by subcutaneous injection. The general state of pregnant mice was observed daily, and their body mass was weighed once a week. After delivery, pregnant rats were exposed to BPS in different groups during lactation period, and the mothers were killed after lactation period. After lactation, F1 mice were observed in general state every day and weighed once a week. After 3 weeks of breastfeeding, F1 mice were fed adaptive diet for 1 week, followed by high-fat diet for 8 weeks. Mouse feces were collected, and mouse heart, liver, pancreas, white adipose and brown adipose were collected. After sampling, the mice were quickly washed in normal saline. After cleaning, the mice were treated with liquid nitrogen and finally frozen in the refrigerator at -80 ℃.

Sample Preparation:

Sampleprep ID:	SP003532
Sampleprep Summary:	Each replicate of mouse (1 tissue, ~15 mg) was accurately weighted and transferred to an Eppendorf tube. Then, each sample was added 200 μL of Milli-Q water,followed by tissue homogenization using the bead-based homogenizer (Tissuelyser-24, Shanghai jingxin industrial development co., ltd, China) for 2 min at 65 Hz twice and the other samples including white adipose and brown adipose were homogenize for 2 min at 65 Hz four times. Next, each sample was added with 800 μL of precooled MeOH/ACN（1:1,v/v）containing standards (i.e., 0.49 μg/mL of Phenylalanine-d5(Phe-d5), 0.50 μg/mL of Tryptophan-d5(Trp-d5), 0.25 μg/mL of Carnitine C2-d3, 0.40 μg/mL of Cholic acid-d4(CA-d4), 0.24 μg/mL of Chenodeoxycholic acid-d4(CDCA-d4), 0.50 μg/mL of palmitic acid (fatty acid (FA) (16:0))-d3, and 0.5 μg/mL of stearic acid (FA(18:0))-d3)，the mixture was thoroughly vortexed for 30 s, use ultrasonic cleaning machine ice bath for 10 min, stand at -20 °C for 1 h, centrifugation at 15,000 g for 15 min at 4 ℃. The supernatant was collected and vacuum-dried in a refrigerated CentriVap concentrator (Labconco, USA), followed by storage at -80 °C until subsequent analysis.

Sampleprep ID:

SP003532

Sampleprep Summary:

Each replicate of mouse (1 tissue, ~15 mg) was accurately weighted and transferred to an Eppendorf tube. Then, each sample was added 200 μL of Milli-Q water,followed by tissue homogenization using the bead-based homogenizer (Tissuelyser-24, Shanghai jingxin industrial development co., ltd, China) for 2 min at 65 Hz twice and the other samples including white adipose and brown adipose were homogenize for 2 min at 65 Hz four times. Next, each sample was added with 800 μL of precooled MeOH/ACN（1:1,v/v）containing standards (i.e., 0.49 μg/mL of Phenylalanine-d5(Phe-d5), 0.50 μg/mL of Tryptophan-d5(Trp-d5), 0.25 μg/mL of Carnitine C2-d3, 0.40 μg/mL of Cholic acid-d4(CA-d4), 0.24 μg/mL of Chenodeoxycholic acid-d4(CDCA-d4), 0.50 μg/mL of palmitic acid (fatty acid (FA) (16:0))-d3, and 0.5 μg/mL of stearic acid (FA(18:0))-d3)，the mixture was thoroughly vortexed for 30 s, use ultrasonic cleaning machine ice bath for 10 min, stand at -20 °C for 1 h, centrifugation at 15,000 g for 15 min at 4 ℃. The supernatant was collected and vacuum-dried in a refrigerated CentriVap concentrator (Labconco, USA), followed by storage at -80 °C until subsequent analysis.

Combined analysis:

Analysis ID	AN005580	AN005581
Analysis type	MS	MS
Chromatography type	Reversed phase	Reversed phase
Chromatography system	Thermo Dionex Ultimate 3000	Thermo Dionex Ultimate 3000
Column	Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um)	ACE Excel 2 C18-PFP (100 x 2.1mm,2um)
MS Type	ESI	ESI
MS instrument type	Orbitrap	Orbitrap
MS instrument name	Thermo Q Exactive HF-X Orbitrap	Thermo Q Exactive HF-X Orbitrap
Ion Mode	POSITIVE	NEGATIVE
Units	Peak area	Peak area

Chromatography:

Chromatography ID:	CH004240
Chromatography Summary:	Positive
Instrument Name:	Thermo Dionex Ultimate 3000
Column Name:	Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um)
Column Temperature:	50
Flow Gradient:	First maintain 2% B for 0.5 minutes, then linearly increase from 0.5 to 12 minutes to 98% B, then rapidly decrease to 2% B at 0.1 minutes, maintain for 8.5 minutes,equilibrate until the next injection
Flow Rate:	0.4 mL/min
Solvent A:	100% water; 0.1% formic acid
Solvent B:	100% acetonitrile; 0.1% formic acid
Chromatography Type:	Reversed phase

Chromatography ID:	CH004241
Chromatography Summary:	Negative
Instrument Name:	Thermo Dionex Ultimate 3000
Column Name:	ACE Excel 2 C18-PFP (100 x 2.1mm,2um)
Column Temperature:	50
Flow Gradient:	First maintain 2% B for 0.5 minutes, then linearly increase from 0.5 to 12 minutes to 98% B, then rapidly decrease to 2% B at 0.1 minutes, maintain for 8.5 minutes,equilibrate until the next injection
Flow Rate:	0.4 mL/min
Solvent A:	100% water; 0.01% ammonia; 5mM ammonium bicarbonate
Solvent B:	50% Methanol/50% Acetonitrile; 0.01% ammonia; 5mM ammonium bicarbonate
Chromatography Type:	Reversed phase

MS:

MS ID:	MS005305
Analysis ID:	AN005580
Instrument Name:	Thermo Q Exactive HF-X Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Xcalibular
Ion Mode:	POSITIVE

MS ID:	MS005306
Analysis ID:	AN005581
Instrument Name:	Thermo Q Exactive HF-X Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Xcalibular
Ion Mode:	NEGATIVE