Summary of Study ST003403
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002107. The data can be accessed directly via it's Project DOI: 10.21228/M88C05 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003403 |
| Study Title | The double-edged role of FASII regulator FabT in Streptococcus pyogenes infection - Metabolomics |
| Study Summary | In Streptococcus pyogenes, the type II fatty acid (FA) synthesis pathway FASII is feedback-controlled by the FabT repressor. A FabT mutation leads to bacterial growth defect in the presence of eukaryotic cells and their supernatant. We investigated a possible metabolic basis for the mFabT growth defect, which could reflect an incapacity to use cell-secreted products for growth. We used a metabolomics approach to assess metabolites that are differentially consumed by mFabT compared to the WT strain, cultivated in the presence of non-infected endometrial cells supernatants (HEC-1-A cells). HEC-1-A supernatants were inoculated or not with WT or mFabT strains during 8 or 16 h. The metabolite composition of these supernatants was analyzed by Proteigene (https://proteigene.com) using MxP® Quant 500 kit (Biocrates) by two analytical methods, LC-MS/MS for small molecules and FIA-MS/MS for lipids. This analysis was repeated on 3 independent series of supernatants. These analyses have a defined detection threshold (LOD) for each family of metabolite. You can found in attachment the raw folders and sample informations. |
| Institute | INSERM |
| Department | U1016 |
| Laboratory | Institut Cochin - Bacteria and perinatality team |
| Last Name | Lambert |
| First Name | Clara |
| Address | 24 rue Méchain 75014 Paris |
| clara.lambert@ibcp.fr | |
| Phone | +33134652168 |
| Submit Date | 2024-08-08 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-08-19 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002107 |
| Project DOI: | doi: 10.21228/M88C05 |
| Project Title: | The double-edged role of FASII regulator FabT in Streptococcus pyogenes infection |
| Project Type: | MS quantitative analysis |
| Project Summary: | In Streptococcus pyogenes, the type II fatty acid (FA) synthesis pathway FASII is feedback-controlled by the FabT repressor. A FabT mutation leads to FASII dysregulation, FAs membrane modification and bacterial growth defect in the presence of eukaryotic cells and their supernatant. We try to understand the consequences on bacteria metabolism. |
| Institute: | INSERM |
| Department: | U1016 |
| Laboratory: | Institut Cochin - Bacteria and perinatality team |
| Last Name: | Lambert |
| First Name: | Clara |
| Address: | 24 rue Méchain 75014 Paris |
| Email: | clara.lambert@ibcp.fr |
| Phone: | +33134652168 |
| Publications: | The double-edged role of FASII regulator FabT in Streptococcus pyogenes infection |
Subject:
| Subject ID: | SU003528 |
| Subject Type: | Bacteria |
| Subject Species: | Streptococcus pyogenes |
| Genotype Strain: | M28PF1 |
| Gender: | Not applicable |
Factors:
Subject type: Bacteria; Subject species: Streptococcus pyogenes (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Treatment |
|---|---|---|---|
| SA371913 | SN inoculated mFabT-16h_1 | Supernatants of HEC-1-A cancer cells | Inoculated with mFabT during 16h |
| SA371914 | SN inoculated mFabT-16h_2 | Supernatants of HEC-1-A cancer cells | Inoculated with mFabT during 16h |
| SA371915 | SN inoculated mFabT-16h_3 | Supernatants of HEC-1-A cancer cells | Inoculated with mFabT during 16h |
| SA371916 | SN inoculated mFabT-8h_1 | Supernatants of HEC-1-A cancer cells | Inoculated with mFabT during 8h |
| SA371917 | SN inoculated mFabT-8h_2 | Supernatants of HEC-1-A cancer cells | Inoculated with mFabT during 8h |
| SA371918 | SN inoculated mFabT-8h_3 | Supernatants of HEC-1-A cancer cells | Inoculated with mFabT during 8h |
| SA371907 | SN inoculated WT-16h_2 | Supernatants of HEC-1-A cancer cells | Inoculated with WT during 16h |
| SA371908 | SN inoculated WT-16h_3 | Supernatants of HEC-1-A cancer cells | Inoculated with WT during 16h |
| SA371909 | SN inoculated WT-16h_1 | Supernatants of HEC-1-A cancer cells | Inoculated with WT during 16h |
| SA371910 | SN inoculated WT-8h_2 | Supernatants of HEC-1-A cancer cells | Inoculated with WT during 8h |
| SA371911 | SN inoculated WT-8h_3 | Supernatants of HEC-1-A cancer cells | Inoculated with WT during 8h |
| SA371912 | SN inoculated WT-8h_1 | Supernatants of HEC-1-A cancer cells | Inoculated with WT during 8h |
| SA371919 | SN_2 | Supernatants of HEC-1-A cancer cells | Non infected |
| SA371920 | SN_3 | Supernatants of HEC-1-A cancer cells | Non infected |
| SA371921 | SN_1 | Supernatants of HEC-1-A cancer cells | Non infected |
| SA371922 | RPMI_1 | Supernatants of HEC-1-A cancer cells | Non infected Non treated |
| SA371923 | RPMI_2 | Supernatants of HEC-1-A cancer cells | Non infected Non treated |
| SA371924 | RPMI_3 | Supernatants of HEC-1-A cancer cells | Non infected Non treated |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO003521 |
| Collection Summary: | HEC-1-A supernatants were inoculated or not with WT or mFabT strains during 8 or 16 h. The metabolite composition of these supernatants was analyzed by Proteigene (https://proteigene.com) using MxP® Quant 500 kit (Biocrates) by two analytical methods, LC-MS/MS for small molecules and FIA-MS/MS for lipids. This analysis was repeated on 3 independent series of supernatants. These analyses have a defined detection threshold (LOD) for each family of metabolite. You can found in attachment the raw folders and sample informations. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR003537 |
| Treatment Summary: | HEC-1-A supernatants were inoculated or not with WT or mFabT strains during 8 or 16 h. |
Sample Preparation:
| Sampleprep ID: | SP003535 |
| Sampleprep Summary: | HEC-1-A supernatants were inoculated or not with WT or mFabT strains during 8 or 16 h. The metabolite composition of these supernatants was analyzed by Proteigene (https://proteigene.com) using MxP® Quant 500 kit (Biocrates) by two analytical methods, LC-MS/MS for small molecules and FIA-MS/MS for lipids. This analysis was repeated on 3 independent series of supernatants. These analyses have a defined detection threshold (LOD) for each family of metabolite. |
Combined analysis:
| Analysis ID | AN005584 | AN005585 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | FIA |
| Chromatography system | Exion LC AD | None |
| Column | Biocrates MxP500 column (50 x 2.1 mm) | NA |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | QTRAP |
| MS instrument name | ABI Sciex 6500+ QTrap | Biocrates Kit |
| Ion Mode | UNSPECIFIED | UNSPECIFIED |
| Units | µM | µM |
Chromatography:
| Chromatography ID: | CH004244 |
| Instrument Name: | Exion LC AD |
| Column Name: | Biocrates MxP500 column (50 x 2.1 mm) |
| Column Temperature: | 50 |
| Flow Gradient: | 0.8ml/min: 0-0.25min: 0%B, 0.25-1.5min: 12%B, 1.5-2.7min: 17.5%B, 2.7-4min: 50%B, 4-4.5min: 100%B, 1ml/min: 4.5-4.7min: 100%B, 4.7-5.0min: 100%B, 5.0-5.1min: 100%B, 0.8ml/min: 5.1-5.8min: 100%B |
| Flow Rate: | 0.8 mL/min |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile; 0.2% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH004245 |
| Instrument Name: | None |
| Column Name: | NA |
| Column Temperature: | NA |
| Flow Gradient: | NA |
| Flow Rate: | NA |
| Solvent A: | NA |
| Solvent B: | NA |
| Chromatography Type: | FIA |
MS:
| MS ID: | MS005309 |
| Analysis ID: | AN005584 |
| Instrument Name: | ABI Sciex 6500+ QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | MS acquisition Comments:Ion Mode was Positive and Negative mode. Software : MetIDQ version Oxygen-DB110-3005 |
| Ion Mode: | UNSPECIFIED |
| MS ID: | MS005310 |
| Analysis ID: | AN005585 |
| Instrument Name: | Biocrates Kit |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | MS acquisition Comments:Ion Mode was Positive and Negative mode. Software : MetIDQ version Oxygen-DB110-3005 |
| Ion Mode: | UNSPECIFIED |
