Summary of Study ST003414
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002113. The data can be accessed directly via it's Project DOI: 10.21228/M8GV5R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003414 |
| Study Title | Metabolic analysis of Anterior Cingulate Cortex in two Autism Spectrum Disorder mouse models |
| Study Summary | In this project, we performed metabolomic analysis of anterior cingulate cortex (ACC) of two autism mouse models (Shank3b-/- and FMR1-/- mice), in comparison with their corresponding wild type controls. The results showed abnormal metabolism of amino acids, especially sulfur containing amino acid, in both Shank3b-/- and FMR1-/- ACC. |
| Institute | Fourth Military Medical University |
| Last Name | Wang |
| First Name | Yazhou |
| Address | 169 Chang Le Xi Road, Xi'an, Shaanxi, 710032, China |
| yazhouw@fmmu.edu.cn | |
| Phone | +86-29-84774562 |
| Submit Date | 2024-08-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-06-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002113 |
| Project DOI: | doi: 10.21228/M8GV5R |
| Project Title: | Metabolic analysis of Anterior Cingulate Cortex in two Autism Spectrum Disorder mouse models |
| Project Summary: | In this project, we performed metabolomic analysis of anterior cingulate cortex (ACC) of two autism mouse models (Shank3b-/- and FMR1-/- mice), in comparison with their corresponding wild type controls. The results showed abnormal metabolism of amino acids, especially sulfur containing amino acid, in both Shank3b-/- and FMR1-/- ACC. |
| Institute: | Fourth Military Medical University |
| Last Name: | Wang |
| First Name: | Yazhou |
| Address: | 169 Chang Le Xi Road, Xi'an, Shaanxi, 710032, China |
| Email: | yazhouw@fmmu.edu.cn |
| Phone: | +86-29-84774562 |
Subject:
| Subject ID: | SU003540 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment | Genotype |
|---|---|---|---|
| SA376460 | FMR1M | KO | FMR1-KO |
| SA376461 | FMR4M | KO | FMR1-KO |
| SA376462 | FMR3M | KO | FMR1-KO |
| SA376463 | FMR2M | KO | FMR1-KO |
| SA376464 | shank1M | KO | Shank3b-KO |
| SA376465 | shank2M | KO | Shank3b-KO |
| SA376466 | shank3M | KO | Shank3b-KO |
| SA376467 | shank4M | KO | Shank3b-KO |
| SA376468 | c57-1M | WT | C57 |
| SA376469 | c57-2M | WT | C57 |
| SA376470 | c57-4M | WT | C57 |
| SA376471 | c57-3M | WT | C57 |
| SA376472 | FVB3M | WT | FVB129 |
| SA376473 | FVB4M | WT | FVB129 |
| SA376474 | FVB2M | WT | FVB129 |
| SA376475 | FVB1M | WT | FVB129 |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO003533 |
| Collection Summary: | Tissues were quickly isolated and individually grounded with liquid nitrogen. |
| Sample Type: | Brain cortex |
Treatment:
| Treatment ID: | TR003549 |
| Treatment Summary: | WT and KO mice were used. |
Sample Preparation:
| Sampleprep ID: | SP003547 |
| Sampleprep Summary: | Tissues were individually grounded with liquid nitrogen and the homogenate was resuspended with 500μL prechilled 80% methanol by well vortex. The samples were incubated on ice for 5 min and then were centrifuged at 15,000 g, 4°C for 20 min. Some of supernatant was diluted to final concentration containing 53% methanol by LC-MS grade water. The samples were subsequently transferred to a fresh Eppendorf tube and then were centrifuged at 15000 g, 4°C for 20 min. |
Chromatography:
| Chromatography ID: | CH004260 |
| Instrument Name: | Vanquish UHPLC |
| Column Name: | Thermo Hypersil GOLD aQ (100 x 2.1mm,1.9um) |
| Column Temperature: | 40° C |
| Flow Gradient: | 0 min, 1% B; 1 min, 1% B; 8 min, 99% B; 10 min, 99% B; 10.1 min, 1% B; 12 min, 1% B. |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN005608 |
| Analysis Type: | MS |
| Chromatography ID: | CH004260 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST003414_AN005608_Results.txt |
| Units: | Peak area |
| Analysis ID: | AN005609 |
| Analysis Type: | MS |
| Chromatography ID: | CH004260 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST003414_AN005609_Results.txt |
| Units: | Peak area |
