Summary of Study ST003478
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002137. The data can be accessed directly via it's Project DOI: 10.21228/M8CZ33 This work is supported by NIH grant, U2C- DK119886.
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| Study ID | ST003478 |
| Study Title | Fatty acid analysis of differently sized MC38 tumors harvested 3 weeks post implantation from control-diet- or high-fat-diet-fed C57BL/6 mice revealed an increase in tumoral oleic acid in high-fat-diet-fed mice. |
| Study Summary | MC38 tumors were harvested 3 weeks post implantation from control-diet- and high-fat-diet-fed mice. At this point, tumors from high-fat-diet-fed mice were larger than that of control-diet-fed mice. These tumors were subject to fatty acid analysis via GC-FID. The resulting data revealed an increase in oleic acid in tumors from high-fat-diet-fed mice. Oleic acid was the only fatty acid to exhibit this trend. |
| Institute | Stanford University |
| Last Name | Bagchi |
| First Name | Sreya |
| Address | 3373 Hillview Avenue |
| sbagchi@stanford.edu | |
| Phone | 4086214773 |
| Submit Date | 2024-09-19 |
| Num Groups | 2 |
| Total Subjects | 7 |
| Analysis Type Detail | GC-FID |
| Release Date | 2024-10-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002137 |
| Project DOI: | doi: 10.21228/M8CZ33 |
| Project Title: | Acid-sensing receptor, GPR65, on tumor macrophages drives accelerated tumor growth in obesity |
| Project Summary: | Multiple cancers, including colorectal cancer (CRC), are more frequent and often more aggressive in obese individuals. Here, we show that macrophages accumulate within tumors of obese CRC patients and in obese CRC mice and promote accelerated tumor growth. These changes are initiated by oleic acid accumulation and subsequent tumor cell-derived acid production, and driven by signaling through GPR65, an acid-sensing receptor on CRC-associated macrophages. We demonstrate a similar role for GPR65 in hepatocellular carcinoma (HCC) in obese mice. Tumors in obese patients with CRC or HCC also exhibit increased GPR65 expression, suggesting that the mechanism revealed here likely contributes to tumor growth in a range of obesity-associated cancers and represents a potential therapeutic target. |
| Institute: | Stanford University |
| Last Name: | Bagchi |
| First Name: | Sreya |
| Address: | 3373 Hillview Avenue |
| Email: | sbagchi@stanford.edu |
| Phone: | 4086214773 |
Subject:
| Subject ID: | SU003606 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 4-5 months |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Treatment |
|---|---|---|---|
| SA383728 | 1 | MC38 murine whole tumor | CD- same size tumor |
| SA383729 | 2 | MC38 murine whole tumor | CD- same size tumor |
| SA383730 | 3 | MC38 murine whole tumor | CD- same size tumor |
| SA383731 | 4 | MC38 murine whole tumor | HFD- same size tumor |
| SA383732 | 5 | MC38 murine whole tumor | HFD- same size tumor |
| SA383733 | 6 | MC38 murine whole tumor | HFD- same size tumor |
| SA383734 | 7 | MC38 murine whole tumor | HFD- same size tumor |
| Showing results 1 to 7 of 7 |
Collection:
| Collection ID: | CO003599 |
| Collection Summary: | MC38 tumors from control and high-fat diet fed mice were collected 7-10 days post tumor implantation. |
| Sample Type: | Whole tumor |
Treatment:
| Treatment ID: | TR003615 |
| Treatment Summary: | C57BL/6 mice were divided into two groups- control diet and high-fat diet. 2 month old mice were fed respective diets from 2 months, at which point MC38 tumors were implanted. |
Sample Preparation:
| Sampleprep ID: | SP003613 |
| Sampleprep Summary: | Whole tumors were resected and immediately frozen and sent for fatty acid analysis. |
| Sampleprep Protocol Filename: | MC38_fatty_acid_GC_protocol.pdf |
Combined analysis:
| Analysis ID | AN005713 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Agilent DB-23 (60m x 0.25mm, 0.25um) |
| MS Type | Other |
| MS instrument type | Other |
| MS instrument name | N/A (FID detection was used) |
| Ion Mode | UNSPECIFIED |
| Units | nmol/mg dry wt |
Chromatography:
| Chromatography ID: | CH004334 |
| Chromatography Summary: | GC-FID was performed on an Agilent 6890N GC coupled to a FID ( flame ionization detector). The FID detector was operated at 260 °C. The hydrogen flow to the detector was 30 mL/min and air flow was 400 mL/min. The sampling rate of the FID was 20 Hz. |
| Methods Filename: | MC38_fatty_acid_GC_protocol.pdf |
| Instrument Name: | Agilent 6890N |
| Column Name: | Agilent DB-23 (60m x 0.25mm, 0.25um) |
| Column Temperature: | 250 |
| Flow Gradient: | Temperature-programmed GC |
| Flow Rate: | 1.5 mL/min |
| Internal Standard: | pentadecanoic acid (15:0) |
| Solvent A: | 3 M methanolic hydrochloric acid |
| Solvent B: | 100% hexane |
| Chromatography Type: | GC |
MS:
| MS ID: | MS005436 |
| Analysis ID: | AN005713 |
| Instrument Name: | N/A (FID detection was used) |
| Instrument Type: | Other |
| MS Type: | Other |
| MS Comments: | No MS involved (FID detection was used) |
| Ion Mode: | UNSPECIFIED |
