Summary of Study ST003480

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002137. The data can be accessed directly via it's Project DOI: 10.21228/M8CZ33 This work is supported by NIH grant, U2C- DK119886.

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Study IDST003480
Study TitleFatty acid analysis of human colorectal adenocarcinoma tumors of normal weight (BMI<25) and obese (BMI>30) patients revealed an increase in oleic acid in obese patients.
Study SummaryFrozen human colorectal adenocarcinoma tumors samples were acquired and analyzed for fatty acid composition via GC-FID. These data revealed an increase in several fatty acids, such as palmitic acid, stearic acid, oleic acid, etc., in obese patients. However, oleic acid was the most abundant fatty acid in tumors of obese patients and also the fatty acid that exhibited the most significant increase in obese patients compared to normal-weight patients.
Institute
Stanford University
Last NameBagchi
First NameSreya
Address3373 Hill Avenue, Palo Alto, CA, 94304, USA
Emailbagchipuja@gmail.com
Phone4086214773
Submit Date2024-08-12
Analysis Type DetailOther
Release Date2024-10-18
Release Version1
Sreya Bagchi Sreya Bagchi
https://dx.doi.org/10.21228/M8CZ33
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002137
Project DOI:doi: 10.21228/M8CZ33
Project Title:Acid-sensing receptor, GPR65, on tumor macrophages drives accelerated tumor growth in obesity
Project Summary:Multiple cancers, including colorectal cancer (CRC), are more frequent and often more aggressive in obese individuals. Here, we show that macrophages accumulate within tumors of obese CRC patients and in obese CRC mice and promote accelerated tumor growth. These changes are initiated by oleic acid accumulation and subsequent tumor cell-derived acid production, and driven by signaling through GPR65, an acid-sensing receptor on CRC-associated macrophages. We demonstrate a similar role for GPR65 in hepatocellular carcinoma (HCC) in obese mice. Tumors in obese patients with CRC or HCC also exhibit increased GPR65 expression, suggesting that the mechanism revealed here likely contributes to tumor growth in a range of obesity-associated cancers and represents a potential therapeutic target.
Institute:Stanford University
Last Name:Bagchi
First Name:Sreya
Address:3373 Hillview Avenue
Email:sbagchi@stanford.edu
Phone:4086214773

Subject:

Subject ID:SU003608
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Group
SA383752Sample 10 - NormalColorectal adenocarcinoma tumor Non-obese
SA383753Sample 2 - NormalColorectal adenocarcinoma tumor Non-obese
SA383754Sample 14 - NormalColorectal adenocarcinoma tumor Non-obese
SA383755Sample 13 - NormalColorectal adenocarcinoma tumor Non-obese
SA383756Sample 12 - NormalColorectal adenocarcinoma tumor Non-obese
SA383757Sample 11 - NormalColorectal adenocarcinoma tumor Non-obese
SA383758Sample 1 - NormalColorectal adenocarcinoma tumor Non-obese
SA383759Sample 9 - NormalColorectal adenocarcinoma tumor Non-obese
SA383760Sample 7 - NormalColorectal adenocarcinoma tumor Non-obese
SA383761Sample 6 - NormalColorectal adenocarcinoma tumor Non-obese
SA383762Sample 5 - NormalColorectal adenocarcinoma tumor Non-obese
SA383763Sample 4 - NormalColorectal adenocarcinoma tumor Non-obese
SA383764Sample 3 - NormalColorectal adenocarcinoma tumor Non-obese
SA383765Sample 8 - NormalColorectal adenocarcinoma tumor Non-obese
SA383766Sample 24 - ObeseColorectal adenocarcinoma tumor Obese
SA383767Sample 29 - ObeseColorectal adenocarcinoma tumor Obese
SA383768Sample 28 - ObeseColorectal adenocarcinoma tumor Obese
SA383769Sample 27 - ObeseColorectal adenocarcinoma tumor Obese
SA383770Sample 26 - ObeseColorectal adenocarcinoma tumor Obese
SA383771Sample 25 - ObeseColorectal adenocarcinoma tumor Obese
SA383772Sample 20 - ObeseColorectal adenocarcinoma tumor Obese
SA383773Sample 23 - ObeseColorectal adenocarcinoma tumor Obese
SA383774Sample 22 - ObeseColorectal adenocarcinoma tumor Obese
SA383775Sample 21 - ObeseColorectal adenocarcinoma tumor Obese
SA383776Sample 19 - ObeseColorectal adenocarcinoma tumor Obese
SA383777Sample 18 - ObeseColorectal adenocarcinoma tumor Obese
SA383778Sample 17 - ObeseColorectal adenocarcinoma tumor Obese
SA383779Sample 15 - ObeseColorectal adenocarcinoma tumor Obese
SA383780Sample 16 - ObeseColorectal adenocarcinoma tumor Obese
Showing results 1 to 29 of 29

Collection:

Collection ID:CO003601
Collection Summary:CRC tumor sample from patients were collected and frozen by the Stanford tissue bank. Samples were collected from non-obese and obese patients.
Sample Type:Colorectal adenocarcinoma tumors

Treatment:

Treatment ID:TR003617
Treatment Summary:Patients were treatment naive.

Sample Preparation:

Sampleprep ID:SP003615
Sampleprep Summary:Tumors were surgically removed and frozen until further use.
Sampleprep Protocol ID:CRC_fatty_acid_protocol.pdf

Combined analysis:

Analysis ID AN005715
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890N
Column Agilent DB-23 (60m x 0.25mm x 0.25um)
MS Type Other
MS instrument type Other
MS instrument name N/A (FID detection was used)
Ion Mode UNSPECIFIED
Units nmol/mg dry wt

Chromatography:

Chromatography ID:CH004336
Chromatography Summary:GC-FID was performed on an Agilent 6890N GC coupled to a FID ( flame ionization detector). The FID detector was operated at 260 °C. The hydrogen flow to the detector was 30 mL/min and air flow was 400 mL/min. The sampling rate of the FID was 20 Hz.
Methods Filename:CRC_fatty_acid_protocol.pdf
Instrument Name:Agilent 6890N
Column Name:Agilent DB-23 (60m x 0.25mm x 0.25um)
Column Temperature:250
Flow Gradient:Temperature-programmed GC
Flow Rate:1.5 mL/min
Internal Standard:pentadecanoic acid (15:0)
Solvent A:3 M methanolic hydrochloric acid
Solvent B:100% hexane
Chromatography Type:GC

MS:

MS ID:MS005438
Analysis ID:AN005715
Instrument Name:N/A (FID detection was used)
Instrument Type:Other
MS Type:Other
MS Comments:No MS involved (FID detection was used)
Ion Mode:UNSPECIFIED
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