Summary of Study ST003481

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002138. The data can be accessed directly via it's Project DOI: 10.21228/M8882W This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003481
Study TitleTherapeutic potential of galactooligosachaccharides and Lactobacillus reuteri supplementation on dextran sulfate sodium-induced gut inflammation
Study SummaryDextran sulfate sodium (DSS)-induced inflammatory bowel disease characterized by intestinal inflammation, barrier dysfunction, and dysbiosis, with limited treatment options available. This study systematically investigates the therapeutic potential of a synbiotic composed of galactooligosaccharides (GOS) and Limosilactobacillus reuteri in a murine model of colitis, revealing that GOS and L. reuteri synergistically protect against intestinal inflammation and barrier dysfunction by promoting the synthesis of pentadecanoic acid, an odd-chain fatty acid, from Bacteroides acidifaciens (B. acidifaciens). Intestinal segments and feces from DSS-treated mice were collected for cytokines detection and metabolomics. Notably, the synbiotic, B. acidifaciens, and pentadecanoic acid are each capable of suppressing intestinal inflammation and enhancing tight junction by inhibiting NF-κB activation. Furthermore, similar reduction in B. acidifaciens and pentadecanoic acid levels are also observed in the feces from both human ulcerative colitis (UC) patients and lipopolysaccharide-induced intestinal inflammation in pigs. Our findings elucidate the protective mechanism of the synbiotic and highlight its therapeutic potential, along with B. acidifaciens and pentadecanoic acid, for intestinal inflammatory disorders.
Institute
China Agricultural University
Last NameWu
First NameYujun
AddressYumingyuan West Road
Emailyujun@cau.edu.cn
Phone18801068572
Submit Date2024-09-02
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailLC-MS
Release Date2024-10-03
Release Version1
Yujun Wu Yujun Wu
https://dx.doi.org/10.21228/M8882W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002138
Project DOI:doi: 10.21228/M8882W
Project Title:Synbiotic galactooligosachaccharides and Lactobacillus reuteri on gut inflammation
Project Summary:Galactooligosaccharides (GOS) and Lactobacillus reuteri are well-known prebiotic and probiotic, respectively, with great benefits in microbial balance and intestinal barrier function. L. reuteri can utilize GOS as a carbon resource, providing the foundation for synbiotic development.It is important to identify the intestinal bacteria and metabolites regulated by the synbiotic and then the beneficial effects of identified intestinal bacteria and metabolites will be evaluated in animals with gut inflammation via cytokines detection and metabolomics.
Institute:China Agricultrual University
Last Name:Wu
First Name:Yujun
Address:Yumingyuan West Road, Beijing, Beijing, 100193, China
Email:yujun@cau.edu.cn
Phone:18801068572

Subject:

Subject ID:SU003609
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Groups Sample source
SA383781A59CON feces
SA383782A431CON feces
SA383783A496CON feces
SA383784A53CON feces
SA383785A244CON feces
SA383786A75CON feces
SA383787A437DSS feces
SA383788A215DSS feces
SA383789A440DSS feces
SA383790A79DSS feces
SA383791A500DSS feces
SA383792A433DSS feces
SA383793A235GL feces
SA383794A20GL feces
SA383795A236GL feces
SA383796A24GL feces
SA383797A21GL feces
SA383798A12GL feces
Showing results 1 to 18 of 18

Collection:

Collection ID:CO003602
Collection Summary:Feces from DSS-treat mice in CON, DSS, and GL group were collected.
Sample Type:Feces
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003618
Treatment Summary:24 8-week male C57BL/6 mice were allocated into three groups: CON, DSS, and GL, respectively treated with PBS, 3%DSS in water supplemented with/without GOS or L.reuteri(CON = Treatment with PBS, DSS = Treatment with 3%DSS, GL = Treatment with 3%DSS+GOS+L.reuteri). After one-week treatment, feces from mice were collected for further metabolomics.

Sample Preparation:

Sampleprep ID:SP003616
Sampleprep Summary:5 mg feces were homogenized in 25 μL water, and the metabolites were extracted with 120 μL ice-cold methanol containing 306 internal standards. Following centrifugation at 18,000 × g for 20 min, 20 μL supernatant was derivatized with prepared derivative reagents on Eppendorf epMotion Workstation

Combined analysis:

Analysis ID AN005716
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity UPLC BEH C18 (100 x 2.1mm, 1.7um); Waters Acquity UPLC BEH C18 Vanguard pre-column (5 x 2.1mm, 1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Waters Xevo-TQ-S
Ion Mode NEGATIVE
Units nmol/g feces

Chromatography:

Chromatography ID:CH004337
Chromatography Summary:ACQUITY UPLC BEH C18 1.7 µM VanGuard pre-column (2.1×5 mm) and ACQUITY UPLC BEH C18 1.7 µM analytical column (2.1 × 100 mm)
Instrument Name:Waters Acquity
Column Name:Waters Acquity UPLC BEH C18 (100 x 2.1mm, 1.7um); Waters Acquity UPLC BEH C18 Vanguard pre-column (5 x 2.1mm, 1.7um)
Column Temperature:40
Flow Gradient:0-1 min (5% B), 1-11min (5-78% B), 11-13.5 min (78-95% B), 13.5-14 min (95-100% B), 14-16 min (100% B)， 16-16.1 min (100-5% B)， 16.1-18 min (5% B)
Flow Rate:0.40 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:70% acetonitrile/30% Isopropanol
Chromatography Type:Reversed phase

MS:

MS ID:MS005439
Analysis ID:AN005716
Instrument Name:Waters Xevo-TQ-S
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:MS settings: Capillary (Kv),1.5 (ESI+), 2.0 (ESI-); Source Temperature, 150℃; Desolvation Temperature, 550℃; Desolvation Gas Flow, 1000 L/Hr. The raw data files generated by UPLC-MS/MS were processed using the TMBQ software (v1.0, Metabo-Profile, Shanghai, China) to perform peak integration, calibration, and quantitation for each metabolite. The self-developed platform iMAP（v1.0, Metabo-Profile, Shanghai, China） was used for statistical analyses, including PCA, OPLS-DA, univariate analysis and pathway analysis.
Ion Mode:NEGATIVE
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