Summary of Study ST003482

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002139. The data can be accessed directly via it's Project DOI: 10.21228/M84F91 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003482
Study Title	Tissue niche influences immune and metabolic profiles to Staphylococcus aureus biofilm infection (Intracellular data)
Study Summary	Infection is a devastating post-surgical complication, often requiring additional procedures and prolonged antibiotic therapy. This is especially relevant for craniotomy and prosthetic joint infections (PJI), both of which are characterized by biofilm formation on the bone or implant surface, respectively, with S. aureus representing a primary cause. The local tissue microenvironment likely has profound effects on immune attributes that can influence treatment efficacy, which becomes critical to consider when developing therapeutics for biofilm infections. However, the extent to which distinct tissue niches influence immune function during biofilm development remains relatively unknown. To address this, we compare the metabolomic, transcriptomic, and functional attributes of leukocytes in mouse models of S. aureus craniotomy and PJI complemented with patient samples from both infection modalities, which reveals profound tissue niche-dependent differences in nucleic acid, amino acid, and lipid metabolism with links to immune modulation. These signatures are both spatially and temporally distinct, differing not only between infection sites but evolving over time within a single model. Collectively, this demonstrates that biofilms elicit unique immune and metabolic responses that are heavily influenced by the local tissue microenvironment, which will likely have important implications when designing therapeutic approaches targeting these infections. This submission contains the intracellular metabolomic data for the larger project.
Institute	University of Nebraska Medical Center
Department	Department of pathology, microbiology and immunology
Last Name	Shinde
First Name	Dhananjay
Address	DRC 2, 7066, UNMC, Emily st
Email	dhananjay.shinde@unmc.edu
Phone	4025597623
Submit Date	2024-09-14
Raw Data Available	Yes
Raw Data File Type(s)	mzXML
Analysis Type Detail	LC-MS
Release Date	2024-09-24
Release Version	1

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Project:

Project ID:	PR002139
Project DOI:	doi: 10.21228/M84F91
Project Title:	Tissue niche influences immune and metabolic profiles to Staphylococcus aureus biofilm infection
Project Type:	Intracellular data
Project Summary:	Infection is a devastating post-surgical complication, often requiring additional procedures and prolonged antibiotic therapy. This is especially relevant for craniotomy and prosthetic joint infections (PJI), both of which are characterized by biofilm formation on the bone or implant surface, respectively, with S. aureus representing a primary cause. The local tissue microenvironment likely has profound effects on immune attributes that can influence treatment efficacy, which becomes critical to consider when developing therapeutics for biofilm infections. However, the extent to which distinct tissue niches influence immune function during biofilm development remains relatively unknown. To address this, we compare the metabolomic, transcriptomic, and functional attributes of leukocytes in mouse models of S. aureus craniotomy and PJI complemented with patient samples from both infection modalities, which reveals profound tissue niche-dependent differences in nucleic acid, amino acid, and lipid metabolism with links to immune modulation. These signatures are both spatially and temporally distinct, differing not only between infection sites but evolving over time within a single model. Collectively, this demonstrates that biofilms elicit unique immune and metabolic responses that are heavily influenced by the local tissue microenvironment, which will likely have important implications when designing therapeutic approaches targeting these infections.
Institute:	UNMC
Department:	Department of pathology, microbiology and immunology
Laboratory:	Pathology, Microbiology and Immunology
Last Name:	Dhananjay
First Name:	Shinde
Address:	DRC 2, 7066, UNMC, Emily st
Email:	dhananjay.shinde@unmc.edu
Phone:	4025597623

Subject:

Subject ID:	SU003610
Subject Type:	Mammal
Subject Species:	Mus musculus
Gender:	Male and female

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	Sample source	Experimental Variable
SA383799	d14-Crain_5	Galea	d14
SA383800	d14-Crain_4	Galea	d14
SA383801	d14-Crain_3	Galea	d14
SA383802	d14-Crain_2	Galea	d14
SA383803	d14-Crain_1	Galea	d14
SA383804	d3-Crain_2	Galea	d3
SA383805	d3-Crain_1	Galea	d3
SA383806	d3-Crain_5	Galea	d3
SA383807	d3-Crain_3	Galea	d3
SA383808	d3-Crain_4	Galea	d3
SA383809	d7-Crain_1	Galea	d7
SA383810	d7-Crain_2	Galea	d7
SA383811	d7-Crain_3	Galea	d7
SA383812	d7-Crain_4	Galea	d7
SA383813	d7-Crain_5	Galea	d7
SA383814	d14-PJI_1	Tissue (PJI)	d14
SA383815	d14-PJI_4	Tissue (PJI)	d14
SA383816	d14-PJI_3	Tissue (PJI)	d14
SA383817	d14-PJI_2	Tissue (PJI)	d14
SA383818	d14-PJI_5	Tissue (PJI)	d14
SA383819	d3-PJI_5	Tissue (PJI)	d3
SA383820	d3-PJI_2	Tissue (PJI)	d3
SA383821	d3-PJI_1	Tissue (PJI)	d3
SA383822	d3-PJI_3	Tissue (PJI)	d3
SA383823	d3-PJI_4	Tissue (PJI)	d3
SA383824	d7-PJI_5	Tissue (PJI)	d7
SA383825	d7-PJI_4	Tissue (PJI)	d7
SA383826	d7-PJI_3	Tissue (PJI)	d7
SA383827	d7-PJI_2	Tissue (PJI)	d7
SA383828	d7-PJI_1	Tissue (PJI)	d7

Showing results 1 to 30 of 30

Showing results 1 to 30 of 30

Collection:

Collection ID:	CO003603
Collection Summary:	The intracellular metabolome of granulocytes isolated from the galea and PJI tissue of mouse craniotomy and PJI models, respectively, were profiled by LC-HRMS. Fifteen mice were infected for each biofilm model (craniotomy and PJI) with single-cell suspensions prepared by pooling tissue homogenates from 3 mice into one sample, resulting in 5 biological replicates. Samples were then enriched for granulocytes using Ly6G magnetic beads (Miltenyi Biotec, Bergishch Gladbach, Germany). Magnetic beads were utilized for purification instead of FACS to limit alterations in cellular metabolism that may occur with extended processing times. Samples were washed twice in 1X PBS, resuspended in 80% MeOH, and frozen at -80°C for 15 min. Metabolomics was also performed on supernatants prepared from brain, galea, and PJI tissue homogenates at days 3, 7, and 14 post-infection. Similar to granulocyte intracellular metabolite collection, tissue supernatants were centrifuged at high speed to remove residual cellular material, combined with 100% MeOH to yield an 80% MeOH solution, and frozen at -80°C.
Sample Type:	Extracellular infection sites homogenate, intracellular granulocyte

Treatment:

Treatment ID:	TR003619
Treatment Summary:	All mice were group-housed at 21-23°C (22°C average) and 30-70% humidity (55% average) under 12 h light/dark cycle with free access to food (2019S Teklad Global 19% Protein Extruded Rodent Diet; Inotiv, West Lafayette, IN) and water. S. aureus craniotomy infection was established in male and female 8- to 10-week-old C57BL/6J mice (RRID:IMSR_JAX:000664). Briefly, ketamine and xylazine were administered to achieve a surgical plane of anesthesia before skin disinfection with betadine. A midline incision of the scalp was made, whereupon a bone flap was created using a high-speed pneumatic drill (Stryker Corporation, Kalamazoo, MI). The bone flap was incubated with 0.5 mL of S. aureus USA300 LAC13C diluted to 10^6/mL in brain-heart infusion broth at 37°C for 5 min and subsequently rinsed in PBS, blotted dry on a sterile field, and reimplanted. This procedure reliably produces an infectious inoculum of 10^3 CFUs per bone flap, which more accurately models surgical site infection that progresses to biofilm formation. The scalp incision was closed using 6-0 nylon suture and animals were continuously monitored until they regained ambulation, and daily thereafter. S. aureus PJI was established in male and female 8- to 10-week-old C57BL/6J mice. Mice were anesthetized with ketamine and xylazine prior to skin disinfection with betadine and initial incision of the quadriceps. A secondary incision was made to laterally displace the patellar tendon and a burr hole was created in the distal end of the femur with a 26-gauge needle. An orthopedic-grade nickel-titanium Kirschner wire (0.6 mm diameter; Custom Wire Technologies, Port Washington, WI) was inserted through the burr hole into the medullary canal and 10^3 CFUs of S. aureus USA300 LAC13C were inoculated at the tip of the wire and the incision was closed with nylon suture. Mice received buprenorphine SR to alleviate any post-surgical pain and were continuously monitored until ambulatory and daily thereafter.

Treatment ID:

TR003619

Treatment Summary:

All mice were group-housed at 21-23°C (22°C average) and 30-70% humidity (55% average) under 12 h light/dark cycle with free access to food (2019S Teklad Global 19% Protein Extruded Rodent Diet; Inotiv, West Lafayette, IN) and water. S. aureus craniotomy infection was established in male and female 8- to 10-week-old C57BL/6J mice (RRID:IMSR_JAX:000664). Briefly, ketamine and xylazine were administered to achieve a surgical plane of anesthesia before skin disinfection with betadine. A midline incision of the scalp was made, whereupon a bone flap was created using a high-speed pneumatic drill (Stryker Corporation, Kalamazoo, MI). The bone flap was incubated with 0.5 mL of S. aureus USA300 LAC13C diluted to 10^6/mL in brain-heart infusion broth at 37°C for 5 min and subsequently rinsed in PBS, blotted dry on a sterile field, and reimplanted. This procedure reliably produces an infectious inoculum of 10^3 CFUs per bone flap, which more accurately models surgical site infection that progresses to biofilm formation. The scalp incision was closed using 6-0 nylon suture and animals were continuously monitored until they regained ambulation, and daily thereafter. S. aureus PJI was established in male and female 8- to 10-week-old C57BL/6J mice. Mice were anesthetized with ketamine and xylazine prior to skin disinfection with betadine and initial incision of the quadriceps. A secondary incision was made to laterally displace the patellar tendon and a burr hole was created in the distal end of the femur with a 26-gauge needle. An orthopedic-grade nickel-titanium Kirschner wire (0.6 mm diameter; Custom Wire Technologies, Port Washington, WI) was inserted through the burr hole into the medullary canal and 10^3 CFUs of S. aureus USA300 LAC13C were inoculated at the tip of the wire and the incision was closed with nylon suture. Mice received buprenorphine SR to alleviate any post-surgical pain and were continuously monitored until ambulatory and daily thereafter.

Sample Preparation:

Sampleprep ID:	SP003617
Sampleprep Summary:	A 13C15N-labeled canonical amino acid (13C15N-CAA) mix (Cambridge Isotope Laboratories, Andover, MA) was added to the extraction buffer comprised of 80% MeOH as an internal standard. Samples were then pelleted, and supernatants were dried by vacuum centrifuge for storage at -80°C. Cell pellets were stored for protein quantification to normalize metabolite peak areas. Prior to LC-HRMS, samples were reconstituted in 100 µL of 50% MeOH, pelleted, and the resulting supernatant was analyzed.

Sampleprep ID:

SP003617

Sampleprep Summary:

A 13C15N-labeled canonical amino acid (13C15N-CAA) mix (Cambridge Isotope Laboratories, Andover, MA) was added to the extraction buffer comprised of 80% MeOH as an internal standard. Samples were then pelleted, and supernatants were dried by vacuum centrifuge for storage at -80°C. Cell pellets were stored for protein quantification to normalize metabolite peak areas. Prior to LC-HRMS, samples were reconstituted in 100 µL of 50% MeOH, pelleted, and the resulting supernatant was analyzed.

Combined analysis:

Analysis ID	AN005717	AN005718
Analysis type	MS	MS
Chromatography type	HILIC	HILIC
Chromatography system	Thermo Vanquish	Thermo Vanquish
Column	Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)	Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
MS Type	ESI	ESI
MS instrument type	Orbitrap	Orbitrap
MS instrument name	Thermo Orbitrap Exploris 480	Thermo Orbitrap Exploris 480
Ion Mode	NEGATIVE	POSITIVE
Units	Relative intensities	Relative intensities

Chromatography:

Chromatography ID:	CH004338
Chromatography Summary:	Metabolite separation was performed by liquid chromatography using a XBridge Amide analytical column (150 × 2.1mm ID; 1.7µm particle size; Waters Corporation, Milford, MA) and a binary solvent system infused at a flow rate of 0.3 mL/min. A guard XBridge Amide column (20 × 2.1mm ID; 3.5µm particle size; Waters Corporation) was connected in front of the analytical column. Mobile phase A was composed of ammonium acetate and ammonium hydroxide (10 mM each) containing 5% acetonitrile in LC-MS grade water; mobile phase B was 100% LC-MS grade acetonitrile. The pH of mobile phase A was adjusted to 8.0 using glacial acetic acid. The UHPLC pumps were operated in gradient mode. The amide column was maintained at 40°C, and the autosampler temperature was held at 5°C throughout data acquisition. The injection volume for all samples was 5 µl with the 13C15N-CAA mix as the internal standard.
Instrument Name:	Thermo Vanquish
Column Name:	Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:	40°C
Flow Gradient:	Started at 85% of B at 0-0.1 min, 84% B at 0.1-3.0 min; 65% B at 3.0-7.3 min, 60% B at 7.3-12.0 min, 55% B at 12.0-15.0 min, 50% B at 15.0-17.0 min, 50% B at 17.0-20.5 min, 70% B at 20.5-22.0 min. The initial conditions were recovered by 22.0 min to equilibrate the column. Total run time was 30 min.
Flow Rate:	0.3 mL/min
Solvent A:	95% Water/5% Acetonitrile; 10mM Ammonium acetate and 10 mM Ammonium hydroxide
Solvent B:	100% Acetonitrile
Chromatography Type:	HILIC

MS:

MS ID:	MS005440
Analysis ID:	AN005717
Instrument Name:	Thermo Orbitrap Exploris 480
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	A HRMS Orbitrap (Exploris 480; Thermo Fisher Scientific) was operated in polarity switching mode and was used for untargeted metabolomics in a data-dependent MS/MS acquisition mode (DDA). Electrospray ionization (ESI) parameters were optimized as follows: electrospray ion voltage of -2700V and 3500V in negative and positive mode respectively, ion transfer tube temperature was maintained at 400°C, and m/z scan range was 70-1050 Da. Orbitrap resolution for precursor ion scans were maintained at 240,000 for intracellular and 120,000 for extracellular whereas fragment ion scans were maintained at 120,000 and 60,000, for intracellular and extracellular respectively. Normalized collision energies at 30, 50, and 150% were used for fragmentation and data was acquired in profile mode.
Ion Mode:	NEGATIVE

MS ID:	MS005441
Analysis ID:	AN005718
Instrument Name:	Thermo Orbitrap Exploris 480
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	A HRMS Orbitrap (Exploris 480; Thermo Fisher Scientific) was operated in polarity switching mode and was used for untargeted metabolomics in a data-dependent MS/MS acquisition mode (DDA). Electrospray ionization (ESI) parameters were optimized as follows: electrospray ion voltage of -2700V and 3500V in negative and positive mode respectively, ion transfer tube temperature was maintained at 400°C, and m/z scan range was 70-1050 Da. Orbitrap resolution for precursor ion scans were maintained at 240,000 for intracellular and 120,000 for extracellular whereas fragment ion scans were maintained at 120,000 and 60,000, for intracellular and extracellular respectively. Normalized collision energies at 30, 50, and 150% were used for fragmentation and data was acquired in profile mode.
Ion Mode:	POSITIVE