Summary of Study ST003536
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002176. The data can be accessed directly via it's Project DOI: 10.21228/M8BV78 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003536 |
| Study Title | Probiotics and their metabolite spermidine enhance IFN-γ+CD4+ T cell immunity to inhibit hepatitis B virus |
| Study Summary | The therapeutic potential of commensal microbes and their metabolites is promising in the functional cure of chronic hepatitis B virus (HBV) infection, which is defined as HBsAg loss. Here, using both specific-pathogen-free and germ-free mice, we report that probiotics significantly promote the decline of HBsAg and inhibit HBV replication by enhancing intestinal homeostasis and provoking intrahepatic IFN-γ+CD4+ T cell immune response. Depletion of CD4+ T cells or blockage of IFN-γ abolishes probiotics-mediated HBV inhibition. Specifically, probiotics-derived spermidine accumulates in gut and transports to liver, where it exhibits a similar anti-HBV effect. Mechanistically, spermidine enhances IFN-γ+CD4+ T cell immunity by autophagy. Strikingly, administration of probiotics in HBV patients reveals a preliminary trend to accelerate the decline of serum HBsAg. In conclusion, probiotics and theirs derived spermidine promote HBV clearance via autophagy-enhanced IFN-γ+CD4+ T cell immunity, highlighting the therapeutic potential of probiotics and spermidine for the functional cure of HBV patients. |
| Institute | Shandong University |
| Last Name | Wang |
| First Name | Tixiao |
| Address | 44 Wenhua Xi Road, Jinan, Shandong |
| tixwang@163.com | |
| Phone | 18560082747 |
| Submit Date | 2024-10-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-11-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002176 |
| Project DOI: | doi: 10.21228/M8BV78 |
| Project Title: | Metabolomics data of feces from Control and BLE mice |
| Project Summary: | Untargeted metabolomic analysis showed that 38 metabolites were significantly different between control and BLE mice. Among them, oligosaccharides (1-kestose, raffinose, maltotriose), amino acids (N-Acetyl-L-leucine, glutamic acid) and polyamine (spermidine, SPD) were the most varied enriched metabolites. |
| Institute: | Shandong University |
| Last Name: | Wang |
| First Name: | Tixiao |
| Address: | 44# Wenhua Xi Road, Jinan, Shandong Province, 250012, China |
| Email: | tixwang@163.com |
| Phone: | 18560082747 |
Subject:
| Subject ID: | SU003665 |
| Subject Type: | Other organism |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
| Species Group: | Mammals |
Factors:
Subject type: Other organism; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA387535 | B2 | BLE |
| SA387536 | B10 | BLE |
| SA387537 | B9 | BLE |
| SA387538 | B8 | BLE |
| SA387539 | B7 | BLE |
| SA387540 | B6 | BLE |
| SA387541 | B5 | BLE |
| SA387542 | B4 | BLE |
| SA387543 | B1 | BLE |
| SA387544 | C3 | Control |
| SA387545 | C10 | Control |
| SA387546 | C9 | Control |
| SA387547 | C8 | Control |
| SA387548 | C7 | Control |
| SA387549 | C6 | Control |
| SA387550 | C5 | Control |
| SA387551 | C4 | Control |
| SA387552 | C2 | Control |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO003658 |
| Collection Summary: | Male five-week-old C57BL/6J mice were hydrodynamically injected with 6 μg of AAV/HBV1.2 plasmid and gavage fed with probiotics (BLE, PBS as control) daily starting at 3 day post of hydrodynamically injection. Feces collected from Control and BLE mice after 6 weeks. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR003674 |
| Treatment Summary: | Male five-week-old C57BL/6J mice were hydrodynamically injected with 6 μg of AAV/HBV1.2 plasmid and gavage fed with probiotics (BLEl) daily at 3 dpi of hydrodynamically injection. |
Sample Preparation:
| Sampleprep ID: | SP003672 |
| Sampleprep Summary: | Take 50±1mg sample into the 2mL EP tubes, extracted with 0.3mL extraction liquid (VMethanol: VChlorofrom = 3:1), add 20μL of L-2-Chlorophenylalanine (1mg/mL stock in dH2O) as internal standard, vortex mixing for 30s; Homogenized in ball mill for 4min at 45Hz, then ultrasound treated for 5min (incubated in ice water); Centrifuge for 15min at 12000rpm, 4℃; Transfer the supernatant (0.2mL) into a fresh 2mL GC/MS glass vial , take 30μL from each sample and pooling as QC sample. Dry completely in a vacuum concentrator without heating; Add 30μL Methoxy amination hydrochloride (20mg/mL in pyridine) incubated for 30min at 80℃; Add 40μL of the BSTFA regent (1% TMCS, v/v) to the sample aliquots, incubated for 1.5h at 70℃; Add 5μL FAMEs (Standard mixture of fatty acid methyl esters, C8-C16:1mg/mL; C18-C24:0.5mg/mL in chloroform) to the QC sample when cooling to the room temperature; All samples were analyzed by gas chromatograph system coupled with a Pegasus HT time-of-flight mass spectrometer (GC-TOF-MS). |
Chromatography:
| Chromatography ID: | CH004411 |
| Chromatography Summary: | GC-TOF-MS analysis was performed using an Agilent 7890 gas chromatograph system coupled with a Pegasus HT time-of-flight mass spectrometer. The system utilized a DB-5MS capillary column coated with 5% diphenyl cross-linked with 95% dimethylpolysiloxane (30m×250μm inner diameter, 0.25μm film thickness; J&W Scientific, Folsom, CA, USA). A 1μL aliquot of the analyte was injected in splitless mode. Helium was used as the carrier gas, the front inlet purge flow was 3mL min−1, and the gas flow rate through the column was 1mL min−1. The initial temperature was kept at 50°C for 1min, then raised to 310°C at a rate of 10°C min−1, then kept for 8min at 310°C. The injection, transfer line, and ion source temperatures were 280, 280, and 250°C, respectively. The energy was -70eV in electron impact mode. The mass spectrometry data were acquired in full-scan mode with the m/z range of 50-500 at a rate of 20 spectra per second after a solvent delay of 6.27min. |
| Instrument Name: | Agilent 7890B |
| Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| Column Temperature: | 50°C |
| Flow Gradient: | - |
| Flow Rate: | - |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | GC |
Analysis:
| Analysis ID: | AN005808 |
| Analysis Type: | MS |
| Chromatography ID: | CH004411 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST003536_AN005808_Results.txt |
| Units: | peak area |
