Summary of Study ST003633
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002246. The data can be accessed directly via it's Project DOI: 10.21228/M89J9M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003633 |
| Study Title | 1H-NMR-based Metabolomic Analysis of Hypersalinity-Induced Oviparity in Brine Shrimp |
| Study Summary | This study investigated the mechanisms by which high salinity conditions stimulate adult Artemia females to produce diapaused cysts. We used a 1H-NMR-based metabolomic approach to elucidate the metabolic regulation between ovoviviparity and oviparity in Artemia exposed to different salinities. At a salinity of 80 parts per thousand (ppt), 100% of females produced diapaused cysts, compared to 20% at 50 ppt. Metabolic profiling revealed significant alterations in a range of metabolites, including 5,6-dihydrouracil, betaine, and malate, in females undergoing oviparity at 80 ppt compared to ovoviviparity at 30 ppt. Multivariate statistical analyses indicated clear separation between the two reproductive strategies. The up-regulated metabolites in oviparity were involved in significant metabolic pathways, such as β-alanine metabolism and the citrate cycle, highlighting substantial metabolic differences between the two reproductive strategies. These identified metabolic pathways might play crucial roles in the maternal response to high salinity, facilitating embryo protection and enhancing the survival and reproductive success of brine shrimp. These findings provide a basis for further research into the molecular mechanisms underlying Artemia adaptation to high salinity environments. |
| Institute | National Kaohsiung University of Science and Technology |
| Department | Department and Graduate Institute of Aquaculture |
| Laboratory | Lab. of Live Feeds Aquaculture |
| Last Name | Chiu |
| First Name | Kuohsun |
| Address | 142 Hai-tuang Rd., Kaohsiung, Not USCanada, 811, Taiwan |
| kuohsun@nkust.edu.tw | |
| Phone | +886-7-3667141#23708 |
| Submit Date | 2024-12-15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | jdf |
| Analysis Type Detail | NMR |
| Release Date | 2025-01-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002246 |
| Project DOI: | doi: 10.21228/M89J9M |
| Project Title: | 1H-NMR-based Metabolomic Analysis of Hypersalinity-Induced Oviparity in Brine Shrimp |
| Project Summary: | This study investigates the metabolic adjustments in Artemia under hypersaline stress using 1H-NMR-based metabolomics. Hypersalinity is known to trigger diapause in brine shrimp, a reproductive strategy for survival in extreme environments. To elucidate the metabolic responses associated with hypersalinity-induced oviparity, we analyzed brine shrimp samples exposed to normal salinity (30 parts per thousand) and high salinity (80 parts per thousand) conditions. The extracted metabolites were analyzed using a 400 MHz NMR spectrometer, and metabolites were identified and quantified using Chenomx Profiler and the Human Metabolome Database (HMDB). |
| Institute: | National Kaohsiung University of Science and Technology |
| Department: | Department and Graduate Institute of Aquaculture |
| Last Name: | Chiu |
| First Name: | Kuohsun |
| Address: | 142 Hai-tuang Rd., Kaohsiung, Not USCanada, 811, Taiwan |
| Email: | kuohsun@nkust.edu.tw |
| Phone: | +886-7-3667141#23708 |
Subject:
| Subject ID: | SU003763 |
| Subject Type: | Invertebrate |
| Subject Species: | Artemia salina |
| Taxonomy ID: | 85549 |
Factors:
Subject type: Invertebrate; Subject species: Artemia salina (Factor headings shown in green)
| mb_sample_id | local_sample_id | Salinity | Sample source |
|---|---|---|---|
| SA393730 | L1 | 30 ppt | Brine shrimp |
| SA393731 | L2 | 30 ppt | Brine shrimp |
| SA393732 | L3 | 30 ppt | Brine shrimp |
| SA393733 | L4 | 30 ppt | Brine shrimp |
| SA393734 | L5 | 30 ppt | Brine shrimp |
| SA393735 | L6 | 30 ppt | Brine shrimp |
| SA393736 | H1 | 80 ppt | Brine shrimp |
| SA393737 | H2 | 80 ppt | Brine shrimp |
| SA393738 | H3 | 80 ppt | Brine shrimp |
| SA393739 | H4 | 80 ppt | Brine shrimp |
| SA393740 | H5 | 80 ppt | Brine shrimp |
| SA393741 | H6 | 80 ppt | Brine shrimp |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO003756 |
| Collection Summary: | Brine shrimp samples were collected to investigate the metabolic response under different salinity conditions. Two experimental groups were established: Control group (30 ppt): Samples (L1–L6) were maintained under normal salinity conditions (30 parts per thousand, ppt). High salinity group (80 ppt): Samples (H1–H6) were exposed to hypersaline conditions (80 ppt) to induce diapause-related metabolic changes. For each group, six biological replicates were collected. The brine shrimp were maintained in controlled laboratory conditions, and after exposure, samples were quickly harvested and processed as follows: Sample collection: Whole brine shrimp were homogenized in liquid nitrogen. Preservation: Homogenized samples were stored at -80°C until NMR analysis. The collected samples were subjected to 1H-NMR analysis to identify and quantify the metabolic changes under hypersalinity stress. The raw NMR data files are named as L1_Proton.jdf–L6_Proton.jdf (30 ppt) and H1_Proton.jdf–H6_Proton.jdf (80 ppt). |
| Sample Type: | Whole animals |
| Storage Conditions: | Described in summary |
Treatment:
| Treatment ID: | TR003772 |
| Treatment Summary: | Brine shrimp samples were divided into two treatment groups to investigate the metabolic responses under different salinity conditions: Control group (30 ppt): Brine shrimp were maintained under normal salinity conditions of 30 parts per thousand (ppt) as the control group. Hypersalinity group (80 ppt): Brine shrimp were exposed to hypersaline conditions of 80 parts per thousand (ppt) to induce a physiological and metabolic response related to diapause. |
Sample Preparation:
| Sampleprep ID: | SP003770 |
| Sampleprep Summary: | Brine shrimp samples were prepared for 1H-NMR-based metabolomics using the following procedures: Sample Collection and Treatment: Brine shrimp were exposed to two salinity conditions: 30 ppt (control group) for ovoviviparity. 80 ppt (hypersalinity group) for oviparity. After 5 days of treatment, adult female brine shrimp were collected, ensuring the presence of white granular eggs (30 ppt) and green stringy cysts (80 ppt). Sample Homogenization and Extraction: Ten females per group were homogenized in 1 mL of pre-chilled 50% methanol at 4°C to quench metabolic activity. The homogenates were centrifuged at 13,000 × g for 20 minutes at 4°C. The resulting supernatants were collected for further processing. Lyophilization and Reconstitution: The supernatants were lyophilized (freeze-dried) to remove water. Lyophilized samples were reconstituted in 600 µL of 100 mM sodium phosphate buffer prepared in D₂O (pH 7.0), containing 1 mM DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid) as the internal reference for NMR analysis. Sample Preparation for NMR Analysis: The reconstituted samples were transferred to 5-mm NMR tubes for analysis. Samples were stored at -80°C until NMR data acquisition. |
| Extract Storage: | Described in summary |
Analysis:
| Analysis ID: | AN005966 |
| Analysis Type: | NMR |
| Operator Name: | Bo-Hui Yu |
| Data Format: | jdf |
| Num Factors: | 2 |
| Num Metabolites: | 38 |
| Units: | uM |
NMR:
| NMR ID: | NM000299 |
| Analysis ID: | AN005966 |
| Instrument Name: | JEOL-ECZS400 |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Standard Concentration: | 100 mM |
| Spectrometer Frequency: | 400 MHz |
| NMR Solvent: | sodium phosphate buffer in D₂O |
| NMR Tube Size: | 5 mm |
