Summary of Study ST003705

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002300. The data can be accessed directly via it's Project DOI: 10.21228/M8B848 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST003705
Study TitleLipid Metabolite Profiling of nontuberculous mycobacterial pulmonary disease
Study SummaryIn this study, we conducted lipidomic analyses of NTM-PD using lung tissues and serum. First, we aimed to identify characteristic lipid profiles associated with diverse geospatial lesions, enabling correlations with specific pathological findings. Next, we examined how these lesion-specific lipidomic profiles are reflected in serum relative to disease severity. Through these analyses, we sought to deepen our understanding of the role of lipid profiles in the pathophysiology of NTM-PD.
Institute
Seoul National University College of Medicine and Hospital
Last NameJungeun
First NameKim
Address101 Daehak-ro, Jongno-gu, Seoul, Korea
Emailjeunk@snu.ac.kr
Phone+821026965910
Submit Date2025-01-30
Raw Data AvailableYes
Raw Data File Type(s)mzML, wiff
Analysis Type DetailLC-MS
Release Date2025-06-02
Release Version1
Kim Jungeun Kim Jungeun
https://dx.doi.org/10.21228/M8B848
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002300
Project DOI:doi: 10.21228/M8B848
Project Title:Lipid Metabolite Profiling of nontuberculous mycobacterial pulmonary disease
Project Summary:In this study, we conducted lipidomic analyses of NTM-PD using lung tissues and serum. First, we aimed to identify characteristic lipid profiles associated with diverse geospatial lesions, enabling correlations with specific pathological findings. Next, we examined how these lesion-specific lipidomic profiles are reflected in serum relative to disease severity. Through these analyses, we sought to deepen our understanding of the role of lipid profiles in the pathophysiology of NTM-PD.
Institute:Seoul National University College of Medicine and Hospital
Last Name:Jungeun
First Name:Kim
Address:101 Daehak-ro, Jongno-gu, Seoul, Korea
Email:jeunk@snu.ac.kr
Phone:+821026965910

Subject:

Subject ID:SU003837
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Factor Sample source
SA405444B_44Bronchiectasis Serum
SA405445B_49Bronchiectasis Serum
SA405446B_48Bronchiectasis Serum
SA405447B_47Bronchiectasis Serum
SA405448B_46Bronchiectasis Serum
SA405449B_45Bronchiectasis Serum
SA405450B_42Bronchiectasis Serum
SA405451B_43Bronchiectasis Serum
SA405452B_51Bronchiectasis Serum
SA405453B_41Bronchiectasis Serum
SA405454B_40Bronchiectasis Serum
SA405455B_39Bronchiectasis Serum
SA405456B_38Bronchiectasis Serum
SA405457B_37Bronchiectasis Serum
SA405458B_50Bronchiectasis Serum
SA405459B_52Bronchiectasis Serum
SA405460B_35Bronchiectasis Serum
SA405461B_53Bronchiectasis Serum
SA405462B_54Bronchiectasis Serum
SA405463B_55Bronchiectasis Serum
SA405464B_56Bronchiectasis Serum
SA405465B_57Bronchiectasis Serum
SA405466B_58Bronchiectasis Serum
SA405467B_59Bronchiectasis Serum
SA405468B_60Bronchiectasis Serum
SA405469B_61Bronchiectasis Serum
SA405470B_62Bronchiectasis Serum
SA405471B_63Bronchiectasis Serum
SA405472B_64Bronchiectasis Serum
SA405473B_65Bronchiectasis Serum
SA405474B_66Bronchiectasis Serum
SA405475B_36Bronchiectasis Serum
SA405476B_33Bronchiectasis Serum
SA405477B_34Bronchiectasis Serum
SA405478B_16Bronchiectasis Serum
SA405479B_2Bronchiectasis Serum
SA405480B_3Bronchiectasis Serum
SA405481B_4Bronchiectasis Serum
SA405482B_5Bronchiectasis Serum
SA405483B_6Bronchiectasis Serum
SA405484B_7Bronchiectasis Serum
SA405485B_8Bronchiectasis Serum
SA405486B_9Bronchiectasis Serum
SA405487B_10Bronchiectasis Serum
SA405488B_11Bronchiectasis Serum
SA405489B_12Bronchiectasis Serum
SA405490B_13Bronchiectasis Serum
SA405491B_14Bronchiectasis Serum
SA405492B_15Bronchiectasis Serum
SA405493B_17Bronchiectasis Serum
SA405494B_68Bronchiectasis Serum
SA405495B_26Bronchiectasis Serum
SA405496B_32Bronchiectasis Serum
SA405497B_31Bronchiectasis Serum
SA405498B_30Bronchiectasis Serum
SA405499B_29Bronchiectasis Serum
SA405500B_28Bronchiectasis Serum
SA405501B_27Bronchiectasis Serum
SA405502B_25Bronchiectasis Serum
SA405503B_18Bronchiectasis Serum
SA405504B_24Bronchiectasis Serum
SA405505B_23Bronchiectasis Serum
SA405506B_22Bronchiectasis Serum
SA405507B_21Bronchiectasis Serum
SA405508B_20Bronchiectasis Serum
SA405509B_19Bronchiectasis Serum
SA405510B_67Bronchiectasis Serum
SA405511B_70Bronchiectasis Serum
SA405512B_69Bronchiectasis Serum
SA405513B_120Bronchiectasis Serum
SA405514B_106Bronchiectasis Serum
SA405515B_107Bronchiectasis Serum
SA405516B_108Bronchiectasis Serum
SA405517B_109Bronchiectasis Serum
SA405518B_110Bronchiectasis Serum
SA405519B_111Bronchiectasis Serum
SA405520B_112Bronchiectasis Serum
SA405521B_113Bronchiectasis Serum
SA405522B_114Bronchiectasis Serum
SA405523B_115Bronchiectasis Serum
SA405524B_116Bronchiectasis Serum
SA405525B_117Bronchiectasis Serum
SA405526B_118Bronchiectasis Serum
SA405527B_119Bronchiectasis Serum
SA405528B_121Bronchiectasis Serum
SA405529B_104Bronchiectasis Serum
SA405530B_130Bronchiectasis Serum
SA405531B_136Bronchiectasis Serum
SA405532B_135Bronchiectasis Serum
SA405533B_134Bronchiectasis Serum
SA405534B_133Bronchiectasis Serum
SA405535B_132Bronchiectasis Serum
SA405536B_131Bronchiectasis Serum
SA405537B_129Bronchiectasis Serum
SA405538B_122Bronchiectasis Serum
SA405539B_128Bronchiectasis Serum
SA405540B_127Bronchiectasis Serum
SA405541B_126Bronchiectasis Serum
SA405542B_125Bronchiectasis Serum
SA405543B_124Bronchiectasis Serum
Showing page 1 of 4     Results:    1  2  3  4  Next     Showing results 1 to 100 of 333

Collection:

Collection ID:CO003830
Collection Summary:Serum was extracted from blood samples obtained when patients participated in the cohorts. The collected blood samples were incubated at 25°C for 15–30 minutes, then centrifuged at 13,756 g for 20 minutes. The isolated serum (500 μL) was stored at −80°C until further analysis.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR003846
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP003843
Sampleprep Summary:For lipid extraction from serum, the internal standard mixture was added to 20 µL of serum and tissue extracts. Lipids were further extracted using a solution of water: IPA: methyl tert-butyl ether followed by vortexing and centrifugation for 5 minutes at 18,341 xg and 4°C. The upper phase was collected into a new 1.5 mL Eppendorf tube. The collected upper phases were mixed and dried under a nitrogen (N2) gas stream. The dried residue was reconstituted with 0.2 mL of 95% acetonitrile (ACN) containing 10 mM ammonium acetate and transferred into an autosampler vial. Pooled sera and lung tissue extracts were used as quality control samples for signal drift correction.

Chromatography:

Chromatography ID:CH004616
Instrument Name:Waters Acquity
Column Name:Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
Column Temperature:45℃
Flow Gradient:% phase B at 0 minutes, held at 0% phase B for 2 minutes, increased to 80% phase B by 5 minutes, reduced to 0% phase B at 5.1 minutes, and equilibrated at 0% until 8 minutes.
Flow Rate:0.4 mL/min
Solvent A:95% Acetonitrile/5% Water
Solvent B:50% Acetonitrile/50% Water
Chromatography Type:HILIC

Analysis:

Analysis ID:AN006078
Analysis Type:MS
Chromatography ID:CH004616
Num Factors:4
Num Metabolites:463
Rt Units:Minutes
Units:relative intensity
  
Analysis ID:AN006079
Analysis Type:MS
Chromatography ID:CH004616
Num Factors:4
Num Metabolites:166
Rt Units:Minutes
Units:relative intensity
  logo