Summary of Study ST003722
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002311. The data can be accessed directly via it's Project DOI: 10.21228/M8X25V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003722 |
| Study Title | Metabolomic analysis of plasma from control, immobilized and FoxO1-Tg mice. |
| Study Summary | The Forkhead box O (FoxO) transcription factors have been reported to be critical factors for muscle atrophy in a variety of pathological and physiological conditions, and genetically induced FoxO1 overexpression resulted in severe muscle atrophy. To better understand the metabolic changes in plasma during immobilization and FoxO1 overexpression, we performed a metabolomic analysis of plasma from 11-week-old C57BL/6J male mice immobilized for 7 days and from age-matched FoxO1-Tg male mice and age-matched control male mice using CE-TOFMS. Our data show that immobilized mice were characterized by a reduction of many metabolites, including 2-oxoglutaric acid (α-ketoglutarate: α-KG), 2-hydroxyisobutyric acid, and guanidinosuccinic acid, in the plasma. |
| Institute | Kyoto Prefectural University |
| Last Name | Kamei |
| First Name | Yasutomi |
| Address | 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, Kyoto, 606-8522, Japan |
| oyabu0507@gmail.com | |
| Phone | +81-75-703-5661 |
| Submit Date | 2025-01-20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-07-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002311 |
| Project DOI: | doi: 10.21228/M8X25V |
| Project Title: | Metabolomic changes in plasma during immobilization and FoxO1 overexpression in mice. |
| Project Summary: | To better understand the metabolic changes in plasma during immobilization and FoxO1 overexpression, we performed a metabolomic analysis of plasma from 11-week-old C57BL/6J male mice immobilized for 7 days and from age-matched FoxO1-Tg male mice and age-matched control male mice using CE-TOFMS. |
| Institute: | Kyoto Prefectural University |
| Last Name: | Kamei |
| First Name: | Yasutomi |
| Address: | 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, Kyoto, 606-8522, Japan |
| Email: | oyabu0507@gmail.com |
| Phone: | +81-75-703-5661 |
Subject:
| Subject ID: | SU004167 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J and FOXO1-Tg |
| Age Or Age Range: | 11-week-old |
| Weight Or Weight Range: | 24.7 g, 23.0 g, 24.8 g, and 22.5 g for control mice (n = 4) 18.2 g, 22.4 g, 20.3 g, and 20.0 g for FoxO1-Tg mice (n = 4), and 21.4 g, 21.3 g, 22.5 g, and 23.6 g for immobilized mice (n = 4). |
| Gender: | Male |
| Animal Animal Supplier: | CLEA Japan (Tokyo, Japan) |
| Animal Light Cycle: | 12-h light-dark cycle |
| Animal Feed: | CE2 |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Group | Sample source | Genotype | Treatment | Time |
|---|---|---|---|---|---|---|
| SA464406 | C-1 | Control group | Plasma | wild-type | Control | - |
| SA464407 | C-3 | Control group | Plasma | wild-type | Control | - |
| SA464408 | C-4 | Control group | Plasma | wild-type | Control | - |
| SA464409 | C-5 | Control group | Plasma | wild-type | Control | - |
| SA464410 | Tg-1 | FOXO1-Tg group | Plasma | FOXO1-Tg | Control | - |
| SA464411 | Tg-2 | FOXO1-Tg group | Plasma | FOXO1-Tg | Control | - |
| SA464412 | Tg-3 | FOXO1-Tg group | Plasma | FOXO1-Tg | Control | - |
| SA464413 | Tg-5 | FOXO1-Tg group | Plasma | FOXO1-Tg | Control | - |
| SA464414 | P-4 | Plaster group | Plasma | wild-type | immobilization | 7 days |
| SA464415 | P-5 | Plaster group | Plasma | wild-type | immobilization | 7 days |
| SA464416 | P-7 | Plaster group | Plasma | wild-type | immobilization | 7 days |
| SA464417 | P-8 | Plaster group | Plasma | wild-type | immobilization | 7 days |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO004160 |
| Collection Summary: | Blood from 11-week-old C57BL/6J male mice immobilized for 7 days, age-matched FOXO1-Tg male mice, and age-matched control male mice was collected into Venoject II vacuum blood collection tube (blood collection tube) and plasma was separated by centrifugation at 800 × g for 5 min, rapidly frozen in liquid nitrogen, and stored at -80°C (n = 4 mice/group). |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR004176 |
| Treatment Summary: | The right hindlimb of C57BL/6J male mice was immobilized (unloaded) with plaster cast. This procedure prevented movement of the immobilized leg alone. Mice not exposed to atrophic conditions were used as controls. |
Sample Preparation:
| Sampleprep ID: | SP004173 |
| Sampleprep Summary: | 50 µL of plasma sample and 450 µL methanol solution (internal standard concentration: 10 μM) were added to each tube and stirred. To this, 500 μL chloroform and 200 μL Milli-Q water were added, stirred, and centrifuged (2,300 × g, 4°C, 5 min). After centrifugation, 200 μL × 2 of the aqueous phases was transferred to ultrafiltration tubes (MILLIPORE, Ultrafree MC UFC3 LCC centrifugal filter unit 5 KDa). This was centrifuged (9,100 × g, 4°C, 120 min) and subjected to ultrafiltration. The filtrate was dried and redissolved in 25 μL Milli-Q water for for metabolome analysis at Human Metabolome Technologies (HMT). |
Chromatography:
| Chromatography ID: | CH005046 |
| Chromatography Summary: | The column used has a model number TSP050375 from Polymicro Technologies. Specifically, this item was purchased and then cut and processed for use. |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | Fused silica capillary i.d. 50 μm x 80 cm |
| Column Temperature: | N/A |
| Flow Gradient: | N/A |
| Flow Rate: | N/A |
| Solvent A: | Cation Buffer Solution (p/n : H3301-1001) |
| Solvent B: | N/A |
| Chromatography Type: | CE |
| Chromatography ID: | CH005047 |
| Chromatography Summary: | The column used has a model number TSP050375 from Polymicro Technologies. Specifically, this item was purchased and then cut and processed for use. |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | Fused silica capillary i.d. 50 μm x 80 cm |
| Column Temperature: | N/A |
| Flow Gradient: | N/A |
| Flow Rate: | N/A |
| Solvent A: | Anion Buffer Solution (p/n : H3302-1021 for run buffer and p/n : H3302-1022 for rinse buffer) |
| Solvent B: | N/A |
| Chromatography Type: | CE |
Analysis:
| Analysis ID: | AN006640 |
| Analysis Type: | MS |
| Chromatography ID: | CH005046 |
| Num Factors: | 3 |
| Num Metabolites: | 102 |
| Units: | arbitrary unit |
| Analysis ID: | AN006641 |
| Analysis Type: | MS |
| Chromatography ID: | CH005047 |
| Num Factors: | 3 |
| Num Metabolites: | 40 |
| Units: | arbitrary unit |
