Summary of Study ST003733
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002319. The data can be accessed directly via it's Project DOI: 10.21228/M8W245 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
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| Study ID | ST003733 |
| Study Title | Proteomics and metabolomics analysis of Mongolian acupuncture therapy for type 2 diabetes mellitus with atherosclerosis |
| Study Summary | The results of serum biochemical and H&E staining analysis suggest that acupuncture therapy can improve cellular inflammatory factors, kidney function, serum lipid function and upper carotid artery tissue lesions in rat of T2DM with atherosclerosis, which is an effective treatment method. In addition, we applied proteomics and metabolomics to comprehensively demonstrate that Mongolian acupuncture therapy in T2DM with atherosclerosis mainly through regulation of fatty acid metabolism. |
| Institute | Inner Mongolia Medical University |
| Last Name | Li |
| First Name | Xueyong |
| Address | Jinshan Campus, Chilechuan Dairy Development Zone, Hohhot, Inner Mongolia, China, 010110 |
| 1324612868@qq.com | |
| Phone | 18947196075 |
| Submit Date | 2025-02-07 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-03-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002319 |
| Project DOI: | doi: 10.21228/M8W245 |
| Project Title: | Proteomics and metabolomics analysis of Mongolian acupuncture therapy for type 2 diabetes mellitus with atherosclerosis |
| Project Type: | Metabolomics analysis |
| Project Summary: | The results of serum biochemical and H&E staining analysis suggest that acupuncture therapy can improve cellular inflammatory factors, kidney function, serum lipid function and upper carotid artery tissue lesions in rat of T2DM with atherosclerosis, which is an effective treatment method. In addition, we applied proteomics and metabolomics to comprehensively demonstrate that Mongolian acupuncture therapy in T2DM with atherosclerosis mainly through regulation of fatty acid metabolism. |
| Institute: | Inner Mongolia Medical University |
| Department: | School of Mongolian Medicine and Pharmacy |
| Last Name: | Li |
| First Name: | Xueyong |
| Address: | Jinshan Campus, Chilechuan Dairy Development Zone, Hohhot, Inner Mongolia, China, 010110 |
| Email: | 1324612868@qq.com |
| Phone: | 18947196075 |
Subject:
| Subject ID: | SU003865 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Gender: | Male |
Factors:
Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Factor |
|---|---|---|---|
| SA407458 | Acp5 | carotid artery tissue | Acupuncture |
| SA407459 | Acp4 | carotid artery tissue | Acupuncture |
| SA407460 | Acp3 | carotid artery tissue | Acupuncture |
| SA407461 | Acp2 | carotid artery tissue | Acupuncture |
| SA407462 | Acp1 | carotid artery tissue | Acupuncture |
| SA407463 | Control2 | carotid artery tissue | Control |
| SA407464 | Control1 | carotid artery tissue | Control |
| SA407465 | Control5 | carotid artery tissue | Control |
| SA407466 | Control4 | carotid artery tissue | Control |
| SA407467 | Control3 | carotid artery tissue | Control |
| SA407468 | Mol4 | carotid artery tissue | Model |
| SA407469 | Mol3 | carotid artery tissue | Model |
| SA407470 | Mol2 | carotid artery tissue | Model |
| SA407471 | Mol1 | carotid artery tissue | Model |
| SA407472 | Mol5 | carotid artery tissue | Model |
| SA407473 | QC1 | carotid artery tissue | QC |
| SA407474 | QC2 | carotid artery tissue | QC |
| SA407475 | QC3 | carotid artery tissue | QC |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO003858 |
| Collection Summary: | SD male rats (SPF, 130-150 g) were randomly selected as diabetic modals of DM (fed high-sugar and high-fat diet). After 6 weeks of induction of insulin resistance, STZ injection was diluted with sterile sodium citrate buffer (0.1 mol L-1, pH 4.2) and injected into mice at a subpathogenic dose (30 mg kg-1) tail vein to induce the occurrence of diabetes. After injection, caudal vein blood was collected on day 3, day 5 and day 7 respectively, and blood glucose exceeding 16.7 mmol L-1 was successful in the modeling of diabetic rats, and unsuccessful 15 rats were excluded. Then vitamin D3 injection was given to diabetic model rats with a total dose of 6×105 U kg-1 for 3 consecutive days, which damaged the integrity of the arterial wall of the rats, increased endothelial permeability, promoted the immersion and deposition of lipids and calcium in the vascular wall, caused the overload of arterial calcium, and promoted the degeneration and calcification of smooth muscle cells. Rats in the diabetic model group are continued to receive a high-fat diet for 4 weeks. At the end of the experiment, all rats were sacrificed, and the upper carotid artery tissue was sent for examination. |
| Collection Protocol Filename: | Experimental_Protocol_for_Harvesting_Carotid_Artery_from_Rats.pdf |
| Sample Type: | carotid artery tissue |
Treatment:
| Treatment ID: | TR003874 |
| Treatment Summary: | the upper carotid artery tissue of about 1 cm long was quickly taken, rinsed with normal saline, frozen with liquid nitrogen and stored at -80 ℃ until metabolite detection |
Sample Preparation:
| Sampleprep ID: | SP003871 |
| Sampleprep Summary: | Samples were weighed before the extraction of metabolites and dried lyophilized were ground in a 2 mL Eppendorf tube containing a 5 mm tungsten bead for 1 min at 65 Hz in a Grinding Mill. Metabolites were extracted using 1 mL precooled mixtures of methanol and water (v/v,4:1) and then placed for 1 h ultrasonic shaking in ice baths. Subsequently, the mixture was placed at -20°C for 1 h and centrifuged at 14,000 g for 20 min at 4°C. The supernatants were recovered and concentrated to dryness in vacuum. |
Combined analysis:
| Analysis ID | AN006124 | AN006125 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1 mm, 1.8 μm) | Waters ACQUITY UPLC HSS T3 (100 x 2.1 mm, 1.8 μm) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH004651 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1 mm, 1.8 μm) |
| Column Temperature: | 40℃ |
| Flow Gradient: | The gradient was 0% buffer B for 2 min and was linearly increase to 48% in 4 min, and then up to 100% in 4 min and maintained for 2 min, and then decreased to 0% buffer B in 0.1 min, with 3 min re-equilibration period employed. |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% Water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile (ACN) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS005830 |
| Analysis ID: | AN006124 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The electrospray ionization (ESI) with positive-mode and negative mode were applied for MS data acquisition separately. The instrument was set to acquire over the m/z range 70-1050 Da for full MS. The full MS scans were acquired at a resolution of 70,000 at m/z 200, and 17,500 at m/z 200 for MS/MS scan. The maximum injection time was set to for 100 ms for MS and 50 ms for MS/MS. The isolation window for MS2 was set to 2 m/z and the normalized collision energy (stepped) was set as 20, 30 and 40 for fragmentation. The raw MS data were processed using MS-DIAL for peak alignment, retention time correction and peak area extraction. |
| Ion Mode: | POSITIVE |
| MS ID: | MS005831 |
| Analysis ID: | AN006125 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The electrospray ionization (ESI) with positive-mode and negative mode were applied for MS data acquisition separately. The instrument was set to acquire over the m/z range 70-1050 Da for full MS. The full MS scans were acquired at a resolution of 70,000 at m/z 200, and 17,500 at m/z 200 for MS/MS scan. The maximum injection time was set to for 100 ms for MS and 50 ms for MS/MS. The isolation window for MS2 was set to 2 m/z and the normalized collision energy (stepped) was set as 20, 30 and 40 for fragmentation. The raw MS data were processed using MS-DIAL for peak alignment, retention time correction and peak area extraction. |
| Ion Mode: | NEGATIVE |
