Summary of Study ST003776
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002354. The data can be accessed directly via it's Project DOI: 10.21228/M8BZ5J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
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| Study ID | ST003776 |
| Study Title | Investigation of Hepatic Lipid Alterations Following Micro- and Nanoplastic-Ingestion |
| Study Summary | To investigate the metabolic consequences of micro- and nanoplastic (MNP) ingestion, a comprehensive lipidomics analysis of liver tissue was performed. Mice were orally administered 50 nm (nanoplastic) or 500 nm (microplastic) polystyrene particles weekly for 12 weeks. Following the treatment period, liver samples were collected, and lipid profiles were analyzed using lipidomics techniques. The results were compared to a control group of mice that received phosphate-buffered saline (PBS) under identical conditions, allowing for a clear assessment of MNP-induced alterations in hepatic lipid metabolism Following MNP ingestion, lipid class analysis revealed alterations in abundance. While triacylglycerols (TAG) and odd-chain triacylglycerols (TAGodd) levels were reduced in MNP-exposed livers, phosphatidylethanolamine (PE), lysophosphatidylethanolamine (LPE), and sphingomyelin (SM) levels were increased. |
| Institute | University of Bonn |
| Department | Developmental Biology of the Immune System, The Life & Medical Sciences Institute (LIMES) |
| Laboratory | Mass Lab |
| Last Name | Makdissi |
| First Name | Nikola |
| Address | Carl Troll straße 31, Bonn, nordRhein westfalen, 53115, Germany |
| nmakdissi@uni-bonn.de | |
| Phone | 02 28 / 73 - 6 2794 |
| Submit Date | 2025-02-26 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-03-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002354 |
| Project DOI: | doi: 10.21228/M8BZ5J |
| Project Title: | Chronically ingested nano- and microplastics accumulate in macrophages and perturb their core functions |
| Project Summary: | Plastic pollution has emerged as a global environmental crisis of unprecedented scale. Micro- and nano-plastic (MNPS) pervade ecosystems, resulting in persistent exposure for all living organisms, including humans. MNPs have been detected in human blood, and animal studies indicate their ability to cross biological barriers and accumulate in organs such as the liver, spleen, and brain. However, the effects of plastic accumulation on tissue homeostasis and immune cell function remain poorly understood. This study investigates the consequences of chronic polystyrene particle ingestion using a mouse model. Following oral ingestion, MNPs breach tissue barriers and accumulate in multiple organs. Hepatic and splenic macrophages are pivotal in the uptake and retention of MNPs, leading to their long-term persistence in these tissues. This prolonged presence disrupts metabolic homeostasis in the liver and adipose tissues without inducing systemic inflammation. Moreover, MNPs ingestion exacerbates metabolic dysregulation under additional stress, such as a high-fat diet, worsening glucose intolerance. On a cellular level, MNPs accumulation in Kupffer cells, the liver-resident macrophages, impairs their phagocytic function, reducing their ability to clear circulating bacteria. Additionally, MNP-exposed mice exhibit signs of an autoimmune phenotype. Altogether, our results demonstrate the hazardous nature of MNPs ingestion on tissue homeostasis, metabolism, and macrophage functionality. These findings suggest that chronic exposure to plastic particles could contribute to the rising prevalence of environmentally linked metabolic and autoimmune diseases. |
| Institute: | University of Bonn |
| Department: | Developmental Biology of the Immune System, The Life & Medical Sciences Institute (LIMES) |
| Laboratory: | Mass Lab |
| Last Name: | Makdissi |
| First Name: | Nikola |
| Address: | Carl Troll straße 31, Bonn, nordRhein westfalen, 53115, Germany |
| Email: | nmakdissi@uni-bonn.de |
| Phone: | 02 28 / 73 - 6 2794 |
Subject:
| Subject ID: | SU003910 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | condition | Sample source |
|---|---|---|---|
| SA409952 | Sample76 | 500 nm | Liver |
| SA409953 | Sample77 | 500 nm | Liver |
| SA409954 | Sample79 | 500 nm | Liver |
| SA409955 | Sample80 | 500 nm | Liver |
| SA409956 | Sample93 | 500 nm | Liver |
| SA409957 | Sample92 | 500 nm | Liver |
| SA409958 | Sample91 | 500 nm | Liver |
| SA409959 | Sample94 | 500 nm | Liver |
| SA409960 | Sample95 | 500 nm | Liver |
| SA409961 | Sample78 | 500 nm | Liver |
| SA409943 | Sample75 | 50 nm | Liver |
| SA409944 | Sample86 | 50 nm | Liver |
| SA409945 | Sample89 | 50 nm | Liver |
| SA409946 | Sample90 | 50 nm | Liver |
| SA409947 | Sample88 | 50 nm | Liver |
| SA409948 | Sample87 | 50 nm | Liver |
| SA409949 | Sample74 | 50 nm | Liver |
| SA409950 | Sample73 | 50 nm | Liver |
| SA409951 | Sample72 | 50 nm | Liver |
| SA409962 | Sample68 | PBS | Liver |
| SA409963 | Sample85 | PBS | Liver |
| SA409964 | Sample84 | PBS | Liver |
| SA409965 | Sample83 | PBS | Liver |
| SA409966 | Sample69 | PBS | Liver |
| SA409967 | Sample81 | PBS | Liver |
| SA409968 | Sample71 | PBS | Liver |
| SA409969 | Sample70 | PBS | Liver |
| SA409970 | Sample82 | PBS | Liver |
| Showing results 1 to 28 of 28 |
Collection:
| Collection ID: | CO003903 |
| Collection Summary: | MNP ingested livers were collected in cold PBS, then snap frozen in Nitrogen. |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR003919 |
| Treatment Summary: | Mice were orally gavaged with carboxylated micro- (500 nm) and nanoplastic (50 nm) polystyrene particles weekly for 12 weeks. both were given in a concentration of 100mg/kg of body weight |
Sample Preparation:
| Sampleprep ID: | SP003916 |
| Sampleprep Summary: | Lipid extraction and tandem mass spectrometry: To evaluate differences in hepatic lipid metabolism tandem mass spectrometry of extracted lipids was performed. For this purpose, 10 mg liver tissue was homogenized in 500 μL ddH2O on ice. Then, 50 μL of the homogenate was transferred into a fresh Eppendorf tube and 500 μL Extraction Mix (CHCl3/MeOH 1/5 containing the following internal standards: 210 pmol PE(31:1), 396 pmol PC(31:1), 98 pmol PS(31:1), 84 pmol PI(34:0), 56 pmol PA(31:1), 51 pmol PG (28:0), 28 pmol CL(56:0), 39 pmol LPA (17:0), 35 pmol LPC(17:1), 38 pmol LPE (17:0), 32 pmol Cer(17:0), 99 pmol, SM(17:0), 55 pmol GlcCer(12:0), 14 pmol GM3 (18:0-D3), 339 pmol TG(50:1-d4), 111 pmol, CE(17:1), 64 pmol DG(31:1), 103 pmol MG(17:1), 724 pmol Chol(d6) and 45 pmol Car(15:0) was added. After 2 min of sonication in a bath sonicator, the samples were spun at 20,000 g for 2 min. The supernatant was collected in a new Eppendorf tube and 200 μL chloroform and 750 μL of 1 M Ammonium acetate (NH4Ac) in ddH2O were added. Following quick manual shaking, the samples were centrifuged at 20,000 g for 2 min again. The upper phase was carefully removed, and the lower phase was transferred into a new Eppendorf tube. The solvent was evaporated using a SpeedVac Vacuum Concentrator at 45 °C for 20 min. The dried lipids were dissolved in 500 μL Spray Buffer (Isopropanol, Methanol, ddH2O (all MS grade), 10 mM ammonium acetate, 0.1 % acetic acid by sonication for 5 min. Until measurement with a Thermo Q Exactive™ Plus (Thermo Scientific) using positive mode, the samples were stored at -20 °C. Before the acquisition, the samples were sonicated for 5 min. |
Combined analysis:
| Analysis ID | AN006202 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE |
| Units | arbitrary units |
Chromatography:
| Chromatography ID: | CH004705 |
| Instrument Name: | none |
| Column Name: | none |
| Column Temperature: | none |
| Flow Gradient: | none |
| Flow Rate: | none |
| Solvent A: | none |
| Solvent B: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS005906 |
| Analysis ID: | AN006202 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The samples were aquired in direct infusion lipidomics using ESI as an ion source in the positive mode For analysis, the raw spectrum files generated by the instrument were converted to .mzML format and imported into LipidXplorer software (192). The software calibrates the mass spectra using both the sample lipids and the previously added internal standard, allowing for mass-based discrimination of different lipid species. The concentration of each lipid species (pmol) was determined by normalizing its signal intensity to the peak intensity of the internal standard, considering the known concentration of the added internal standard in each sample NOTE: "0" values indicate that the metabolite wasn't detected, |
| Ion Mode: | POSITIVE |
