Summary of Study ST003807

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002381. The data can be accessed directly via it's Project DOI: 10.21228/M8VR88 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST003807
Study Title	A lipidomic exploration of the effects of high-intensity interval exercise in healthy men after metformin intake
Study Summary	Changes in the plasma levels of individual lipids could play an important role in the response to exercise under metformin intake. This study explores the changes in the plasma lipidome of nine healthy individuals for 12 h after metformin intake followed by either high-intensity interval exercise (HIIE) or rest, followed by food intake in both cases. We observed several variations in the lipid profiles due to HIIE. Most affected lipid classes are fatty acids, acyl carnitines, phosphatidylcholines, sphingomyelins, and triglycerides, of which most were increased upon HIIE, but only some (mainly fatty acids and triglycerides) upon food intake.
Institute	University CEU San Pablo
Department	Chemistry and Biochemistry
Laboratory	CEMBIO
Last Name	Neuhaus
First Name	René
Address	Urb. Montepríncipe, Alcorcón, Madrid, 28925, Spain
Email	rene.neuhaus@ceu.es
Phone	+34611042778
Submit Date	2025-03-14
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2025-03-21
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR002381
Project DOI:	doi: 10.21228/M8VR88
Project Title:	A lipidomic exploration of the effects of high-intensity interval exercise in healthy men after metformin intake
Project Type:	Lipidomics untargeted MS
Project Summary:	Changes in the plasma levels of individual lipids could play an important role in the response to exercise under metformin intake. This study explores the changes in the plasma lipidome of nine healthy individuals for 12 h after metformin intake followed by either high-intensity interval exercise (HIIE) or rest, followed by food intake in both cases. We observed several variations in the lipid profiles due to HIIE. Most affected lipid classes are fatty acids, acyl carnitines, phosphatidylcholines, sphingomyelins, and triglycerides, of which most were increased upon HIIE, but only some (mainly fatty acids and triglycerides) upon food intake.
Institute:	University CEU San Pablo
Department:	Chemistry and Biochemistry
Laboratory:	CEMBIO
Last Name:	Neuhaus
First Name:	René
Address:	Urb. Montepríncipe, Alcorcón, Madrid, 28925, Spain
Email:	rene.neuhaus@ceu.es
Phone:	+34611042778

Subject:

Subject ID:	SU003941
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Sample source	Session	Timepoint
SA417101	A7_0	Plasma	Exercise	0
SA417102	A2_0	Plasma	Exercise	0
SA417103	A9_0	Plasma	Exercise	0
SA417104	A8_0	Plasma	Exercise	0
SA417105	A1_0	Plasma	Exercise	0
SA417106	A6_0	Plasma	Exercise	0
SA417107	A5_0	Plasma	Exercise	0
SA417108	A3_0	Plasma	Exercise	0
SA417109	A4_0	Plasma	Exercise	0
SA417110	A1_1	Plasma	Exercise	1
SA417111	A7_1	Plasma	Exercise	1
SA417112	A2_1	Plasma	Exercise	1
SA417113	A8_1	Plasma	Exercise	1
SA417114	A9_1	Plasma	Exercise	1
SA417115	A6_1	Plasma	Exercise	1
SA417116	A5_1	Plasma	Exercise	1
SA417117	A4_1	Plasma	Exercise	1
SA417118	A3_1	Plasma	Exercise	1
SA417119	A3_10	Plasma	Exercise	10
SA417120	A4_10	Plasma	Exercise	10
SA417121	A5_10	Plasma	Exercise	10
SA417122	A6_10	Plasma	Exercise	10
SA417123	A7_10	Plasma	Exercise	10
SA417124	A8_10	Plasma	Exercise	10
SA417125	A9_10	Plasma	Exercise	10
SA417126	A2_10	Plasma	Exercise	10
SA417127	A1_10	Plasma	Exercise	10
SA417128	A9_11	Plasma	Exercise	11
SA417129	A3_11	Plasma	Exercise	11
SA417130	A8_11	Plasma	Exercise	11
SA417131	A2_11	Plasma	Exercise	11
SA417132	A1_11	Plasma	Exercise	11
SA417133	A4_11	Plasma	Exercise	11
SA417134	A5_11	Plasma	Exercise	11
SA417135	A6_11	Plasma	Exercise	11
SA417136	A7_11	Plasma	Exercise	11
SA417137	A3_12	Plasma	Exercise	12
SA417138	A4_12	Plasma	Exercise	12
SA417139	A6_12	Plasma	Exercise	12
SA417140	A7_12	Plasma	Exercise	12
SA417141	A8_12	Plasma	Exercise	12
SA417142	A9_12	Plasma	Exercise	12
SA417143	A1_12	Plasma	Exercise	12
SA417144	A5_12	Plasma	Exercise	12
SA417145	A2_12	Plasma	Exercise	12
SA417146	A9_13	Plasma	Exercise	13
SA417147	A8_13	Plasma	Exercise	13
SA417148	A7_13	Plasma	Exercise	13
SA417149	A6_13	Plasma	Exercise	13
SA417150	A5_13	Plasma	Exercise	13
SA417151	A4_13	Plasma	Exercise	13
SA417152	A3_13	Plasma	Exercise	13
SA417153	A2_13	Plasma	Exercise	13
SA417154	A1_13	Plasma	Exercise	13
SA417155	A5_2	Plasma	Exercise	2
SA417156	A9_2	Plasma	Exercise	2
SA417157	A7_2	Plasma	Exercise	2
SA417158	A6_2	Plasma	Exercise	2
SA417159	A8_2	Plasma	Exercise	2
SA417160	A4_2	Plasma	Exercise	2
SA417161	A2_2	Plasma	Exercise	2
SA417162	A1_2	Plasma	Exercise	2
SA417163	A3_2	Plasma	Exercise	2
SA417164	A5_3	Plasma	Exercise	3
SA417165	A9_3	Plasma	Exercise	3
SA417166	A8_3	Plasma	Exercise	3
SA417167	A7_3	Plasma	Exercise	3
SA417168	A6_3	Plasma	Exercise	3
SA417169	A4_3	Plasma	Exercise	3
SA417170	A3_3	Plasma	Exercise	3
SA417171	A2_3	Plasma	Exercise	3
SA417172	A1_3	Plasma	Exercise	3
SA417173	A9_4	Plasma	Exercise	4
SA417174	A1_4	Plasma	Exercise	4
SA417175	A2_4	Plasma	Exercise	4
SA417176	A3_4	Plasma	Exercise	4
SA417177	A4_4	Plasma	Exercise	4
SA417178	A5_4	Plasma	Exercise	4
SA417179	A6_4	Plasma	Exercise	4
SA417180	A7_4	Plasma	Exercise	4
SA417181	A8_4	Plasma	Exercise	4
SA417182	A2_5	Plasma	Exercise	5
SA417183	A1_5	Plasma	Exercise	5
SA417184	A5_5	Plasma	Exercise	5
SA417185	A3_5	Plasma	Exercise	5
SA417186	A4_5	Plasma	Exercise	5
SA417187	A6_5	Plasma	Exercise	5
SA417188	A7_5	Plasma	Exercise	5
SA417189	A8_5	Plasma	Exercise	5
SA417190	A9_5	Plasma	Exercise	5
SA417191	A1_6	Plasma	Exercise	6
SA417192	A3_6	Plasma	Exercise	6
SA417193	A4_6	Plasma	Exercise	6
SA417194	A5_6	Plasma	Exercise	6
SA417195	A6_6	Plasma	Exercise	6
SA417196	A7_6	Plasma	Exercise	6
SA417197	A8_6	Plasma	Exercise	6
SA417198	A9_6	Plasma	Exercise	6
SA417199	A2_6	Plasma	Exercise	6
SA417200	A2_7	Plasma	Exercise	7

Showing page 1 of 3 Results: 1 2 3 Next Showing results 1 to 100 of 217

Collection:

Collection ID:	CO003934
Collection Summary:	Venous blood samples were collected in EDTA tubes, in the exercise session (A) before taking metformin (0 h, T0) and after taking metformin at 40 min (T1, before exercise), 1 h 25 min (T2, 40 min after start of exercise), 2 h 5 min (T3, 4 min after finishing exercise), 2 h 30 min (T4), 3 h (T5), 3 h 30 min (T6), 4 h (T7, after banana intake), 4 h 30 min (T8), 6 h (T9), 7 h (T10, after meal intake), 8 h (T11), 10 h (T12) and 12 h (T13). In the resting session (C), blood was taken before taking metformin (0 h, T0) and after taking metformin at 40 min (T1), 1 h 25 min (T2), 2 h 5 min (T3), 3 h (T5) and 6 h (T9).
Sample Type:	Blood (plasma)
Storage Conditions:	-80℃

Treatment:

Treatment ID:	TR003950
Treatment Summary:	Plasma was collected by centrifugation and stored at –80ºC

Sample Preparation:

Sampleprep ID:	SP003947
Sampleprep Summary:	Plasma samples were thawed on ice for approximately 1 h and vortex-mixed for 2 min. Then, 50 μL of each sample were transferred to an Eppendorf vial on ice. Subsequently, 175 μL of ice-cold methanol (stored at –20°C), containing 2.3 ppm of sphinganine (d17:0) and 4.6 ppm of palmitic acid-d31. After shaking the sample for 1 min, 175 μL of MTBE and 10 μL of LightSPLASH LIPIDOMIX Quantitative Mass Spec Primary Standard mix were added. Samples were vortex-mixed for 30 min and centrifuged for 15 min at 15°C and 16,100 x g. Finally, 100 μL of supernatants were added to chromatography vials, centrifuged for 5 min at 15°C and 2,000 x g, and then injected into the LC-MS system. Extraction solvents were used as a blank and followed the same procedure as the samples. Quality control (QC) samples were prepared by pooling equal volumes of all samples.

Sampleprep ID:

SP003947

Sampleprep Summary:

Plasma samples were thawed on ice for approximately 1 h and vortex-mixed for 2 min. Then, 50 μL of each sample were transferred to an Eppendorf vial on ice. Subsequently, 175 μL of ice-cold methanol (stored at –20°C), containing 2.3 ppm of sphinganine (d17:0) and 4.6 ppm of palmitic acid-d31. After shaking the sample for 1 min, 175 μL of MTBE and 10 μL of LightSPLASH LIPIDOMIX Quantitative Mass Spec Primary Standard mix were added. Samples were vortex-mixed for 30 min and centrifuged for 15 min at 15°C and 16,100 x g. Finally, 100 μL of supernatants were added to chromatography vials, centrifuged for 5 min at 15°C and 2,000 x g, and then injected into the LC-MS system. Extraction solvents were used as a blank and followed the same procedure as the samples. Quality control (QC) samples were prepared by pooling equal volumes of all samples.

Combined analysis:

Analysis ID	AN006259	AN006260
Analysis type	MS	MS
Chromatography type	Reversed phase	Reversed phase
Chromatography system	Agilent 1290 Infinity II	Agilent 1290 Infinity II
Column	Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3 mm, 2.7 µm)	Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3 mm, 2.7 µm)
MS Type	ESI	ESI
MS instrument type	QTOF	QTOF
MS instrument name	Agilent 6545 QTOF	Agilent 6545 QTOF
Ion Mode	POSITIVE	NEGATIVE
Units	Peak area	Peak area

Chromatography:

Chromatography ID:	CH004748
Chromatography Summary:	Samples were analyzed using an Agilent 1290 Infinity II UHPLC system coupled to an Agilent 6545 quadrupole time-of-flight (QTOF) mass spectrometer (Agilent, Santa Clara, CA, USA). An Agilent InfinityLab Poroshell 120 EC-C18 (3.0 × 100 mm, 2.7 µm) column, protected by a compatible guard column (Agilent InfinityLab Poroshell 120 EC-C18, 3.0 × 5 mm, 2.7 µm), was employed for the analysis. The column temperature was set at 50°C, while the autosampler’s temperature was set at 15°C. Data were acquired in both ionization modes in separate runs. For positive ionization (ESI+), the injection volume was set at 0.5 μL, while, for negative ionization (ESI-), at 1 μL. Elution was performed with a solvent system consisting of (A) 10 mM ammonium acetate, 0.2 mM ammonium fluoride in water/methanol (9:1, v/v) and (B) 10 mM ammonium acetate, 0.2 mM ammonium fluoride in acetonitrile/methanol/isopropanol (2:3:5, v/v/v). The elution program applied was 70% B at 0–1 min, linear gradient to 86% B until 3.5 min, held until 10 min, linear gradient to 100% B until 11 min, and held until 17 min. Then, the system returned to the initial condition for a total of 2 min. The total analysis time was 19 min.
Instrument Name:	Agilent 1290 Infinity II
Column Name:	Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3 mm, 2.7 µm)
Column Temperature:	50ºC
Flow Gradient:	0-1 min: 70% B; 1-3.5 min: linear increase until 86% B; 3.5-10 min: 86% B; 10-11 min: linear increase until 100% B; 11-17 min: 100% B. Then the equipment returned to the initial conditions in 0.1 min, which were held for 1.9 min for column reconditioning.
Flow Rate:	0.6 mL/min
Solvent A:	90% Water/10% Methanol; 10 mM Ammonium acetate; 0.2 mM Ammonium fluoride
Solvent B:	50% Isopropanol/30% Methanol/20% Acetonitrile; 10 mM ammonium acetate; 0.2 mM ammonium fluoride
Chromatography Type:	Reversed phase

MS:

MS ID:	MS005961
Analysis ID:	AN006259
Instrument Name:	Agilent 6545 QTOF
Instrument Type:	QTOF
MS Type:	ESI
MS Comments:	The QTOF mass spectrometer, equipped with a dual atmospheric jet stream electrospray ionization (ESI) ion source, was configured with the following parameters: 150 V fragmentor, 65 V skimmer, 3500 V capillary voltage, 750 V octupole radio frequency voltage, 10 L/min nebulizer gas flow, 200°C gas temperature, 50 psi nebulizer gas pressure, 12 L/min sheath gas flow, and 300°C sheath gas temperature. In full scan mode, operated from 50 to 1.800 m/z with a scan rate of 3 spectra/s, data were collected for both ionization modes. A solution of two reference mass compounds was used throughout the analysis: purine (C5H4N4) at m/z 121.0509 and HP-0921 (C18H18O6N3P3F24) at m/z 922.0098. These masses were continuously infused into the system to provide constant mass correction.
Ion Mode:	POSITIVE

MS ID:	MS005962
Analysis ID:	AN006260
Instrument Name:	Agilent 6545 QTOF
Instrument Type:	QTOF
MS Type:	ESI
MS Comments:	The QTOF mass spectrometer, equipped with a dual atmospheric jet stream electrospray ionization (ESI) ion source, was configured with the following parameters: 150 V fragmentor, 65 V skimmer, 3500 V capillary voltage, 750 V octopole radio frequency voltage, 10 L/min nebulizer gas flow, 200°C gas temperature, 50 psi nebulizer gas pressure, 12 L/min sheath gas flow, and 300°C sheath gas temperature. In full scan mode, operated from 50 to 1.800 m/z with a scan rate of 3 spectra/s, data were collected for both ionization modes. A solution of two reference mass compounds was used throughout the analysis: purine (C5H4N4) at m/z 119.0363 and HP-0921 (C18H18O6N3P3F24) at m/z 980.0163 (HP-0921 + acetate). These masses were continuously infused into the system to provide constant mass correction.
Ion Mode:	NEGATIVE